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Citations for Stemcell Technologies :
501 - 550 of 4606 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Human T cells from anonymous donors sourced by STEMCELL Technologies were isolated by negative selection for CD3+ populations as per manufacturer’s instruction (EasySep ...
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bioRxiv - Immunology 2023Quote: ... or ImmunoCult Human CD3/CD28 T cell activator (Stemcell Technologies) was added at a dilution of 1:500.
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bioRxiv - Molecular Biology 2024Quote: ... Pan-T cells were obtained from StemCell Technologies (Catalog: 70024).
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Microbiology 2020Quote: ... and RosetteSep Human CD8 Depletion Cocktail (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or total T cells using EasySep isolation kits (Catalog Nos. 19765, 19853, and 19851; STEMCELL Technologies Inc., Vancouver, BC, Canada). Negative selection was confirmed by flow cytometry analysis to > 90% purity ...
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bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... coated surface in the absence of feeders and were cultured in mTESR2 (Stem cell technologies) media ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated at 1 × 106 cells/ml in T cell growth media (StemCell Technology, # 10981) and activated using ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (StemCell Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... and activated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Stem Cell Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CD4 T cells (non-irradiated) were isolated by magnetic bead selection (Cell Stemcell Technologies). Cells were cultured for 72 hours and flow cytometric analysis used to assess proliferation as measured by CFSE dilution.
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bioRxiv - Cancer Biology 2022Quote: Primary T cells were isolated from LeukoPaks obtained from Stem Cell Technologies (200-0092). CD8 and CD4 cells were isolated separately using their EasySep™ Human CD8+/CD4+ T Cell Isolation Kit as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated CD4+ T cells were then subjected to a CD25+ cell isolation (STEMCELl Technologies) using releasable magnetic spheres (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Beta Tubulin (STEMCELL Technologies; 1:500); Rabbit anti-Olig2 (PhosphoSolutions ...
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bioRxiv - Systems Biology 2024Quote: ... Mouse and Rat proliferation supplement 10% (Stem Cell Technologies #05701), Recombinant Human FGF (20 ng/mL ...
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bioRxiv - Microbiology 2024Quote: ... Bulk CD4+ T cells were subsequently isolated from PBMCs by magnetic negative selection using an EasySep Human CD4+ T cell isolation kit (STEMCELL Technologies; per the manufacturer’s instructions). Isolated CD4+ T cells were suspended in RPMI 1640 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 24 mM [U-13C]glucose ...
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bioRxiv - Bioengineering 2021Quote: Pan T cells were isolated from donor PBMC’s (#70025, StemCell Technologies) using EasySep™ human T cell isolation kit (#17951 ...
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bioRxiv - Bioengineering 2019Quote: ... and cultured in ImmunoCult-XF T cell Expansion Medium (StemCell Technologies). Approximately 24 hours post-isolation ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using negative selection (STEMCELL Technologies #17952) and were immediately cultured in complete RPMI media (RPMI 1640 (Gibco #22400089 ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... ImmunoCult™ human CD3/CD28/CD2 T-cell activator (StemCell Technologies) was used to activate isolated T cells in vitro for 2 days following the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2024Quote: ... ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada), CD3Ab OKT3 (Sino Biological ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were suspended in CryoStor 10 (StemCell Technologies, Seattle, WA), aliquoted ...
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bioRxiv - Genomics 2024Quote: ... CD3+ T cells were isolated by magnetic bead separation (StemCell Technology) from PBMCs of three leukopaks (Cambridge Biosciences ...
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bioRxiv - Genomics 2024Quote: CD3+ T cells were isolated from healthy donor leukopaks (Stemcell Technologies) using bead separation (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: Pan T cells were isolated (Miltenyi) from PBMCs (Leukopak, StemCell Technologies) and plated in TexMACS or ImmunoCult at 7x105 cells/ml in a 6-well plate ...
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bioRxiv - Cancer Biology 2019Quote: Mouse CD3 positive T cells were isolated from spleens collected from CEA transgenic mice using negative selection kit (Stemcell Technologies, Canada) and plated for activation on 6 well plates previously coated with αCD3 antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... primary naïve CD4+ T cells were isolated from the spleen of OT-II mice (B6.Cg-Tg(TcraTcrb)425Cbn/J) using EasySep Mouse CD4+ T cell isolation kit (Stemcell Technologies) after homogenization with a 70 µm cell strainer and resuspending the cells in PBS supplemented with 2 % FCS and 1 mM EDTA ...
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bioRxiv - Bioengineering 2022Quote: T cells were isolated from human donor whole blood samples by negative selection using the RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies). Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: Primary human CD4+ T cells were isolated from PBMCs using a Human resting CD4+ T Cells Isolation Kit I according to the manufacturer instructions (STEMCELL Technologies). Briefly ...
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bioRxiv - Immunology 2019Quote: ... cryopreserved PBMCs were thawed (as above) and CD4 T cells were isolated by negative selection using the EasySep Human CD4 Enrichment Kit (Stemcell Technologies) before DNA extraction with the QiaAMP Blood Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... Resting CD4+ T cells (rCD4s) were isolated from PBMC by magnetic negative selection using the EasySep Human Resting CD4+ T cell Isolation Kit (StemCell Technologies). In some experiments ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Immunology 2023Quote: ... ETRAMP-specific CD4+ T cells were enriched using APC- and PE-conjugated tetramers and EasySep APC and PE Positive Selection Kits (STEMCELL Technologies). Cells were then stained with fluorescent dye-conjugated antibodies (see Table I ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from peripheral lymph nodes (inguinal, axillary, brachial and superficial cervical) by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies). CD4+ T cells were stimulated in 96 well flat-bottomed plates coated with 20mg/ml αCD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... Cells were left alone or activated using Immunocult human CD3/CD28 T Cell Activator (Stem Cell Technologies) for 15 min ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using the EasySep Biotin Positive Selection Kit (Stemcell Technologies) with anti-human/mouse β7 integrin antibody (FIB504 ...
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bioRxiv - Immunology 2023Quote: ... one piece (∼3g) was used for CD25+CD8- Treg enrichment using a custom thymic Treg selection kit (STEMCELL Technologies)102 ...
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bioRxiv - Cancer Biology 2021Quote: Human Peripheral Blood Pan-T Cells was purchased from Stem Cell Technologies (Vancouver, BC, Canada) and Panc-1 cells were obtained from the Kansas University Medical Center ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were activated with 1X ImmunoCult Human CD3/CD28 T Cell Activator (#10971, Stemcell Technologies) and cultured for 4 days ...
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bioRxiv - Genomics 2022Quote: ... The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies, cat #07930) at −80 °C until use (50,000 cells per tube) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Stimulation was done using ImmunoCult human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies) in non-polarizing conditions using anti-IL-4 (5 μg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Immunology 2022Quote: ... CAR T cells were positively selected for truncated CD19 using the EasySep™ CD19 Positive Enrichment Kit I or II (StemCell Technologies) (for PSCA-CAR T cells ...