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Citations for Stemcell Technologies :
351 - 400 of 4606 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... 5.0 × 106 cryopreserved T cells were expanded using CD3/CD28 T cell activator (StemCell Technologies) and standard conditions for 16 days to achieve sufficient cell numbers for the experimental workflow ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were activated for 72 hours with ImmunoCult T cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Leukopak-derived peripheral blood mononuclear cells (PBMCs) purchased from LifeSouth Community Blood Centers were debulked with RosetteSep CD8+ enrichment kits (Stem Cell Technologies), and then separated into an enriched fraction of PBMC using a Ficoll gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were then isolated using EasySep™ Human T Cell Isolation Kit and EasySep™ Magnet (Stemcell Technologies, Cat. No. 17951 and Cat. No. 18000) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CD8- thymocytes were obtained by negative selection using the EasySep Mouse Streptavidin Rapid Spheres Isolation Kit (Stem Cell Technologies), then subjected to cell sorting ...
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bioRxiv - Cancer Biology 2020Quote: Both CD4+ and CD8+ cells were isolated from normal mouse splenocytes by immune-magnetic negative selection kit (Stemcell Technology, Vancouver ...
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bioRxiv - Immunology 2021Quote: ... followed by enrichment of either effector/memory T cells (Human Memory CD4 T cell Enrichment kit; EasySep) or Treg cells (Human CD4+CD127loCD49d− Treg Enrichment Kit; EasySep) (all from StemCell Technologies).
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bioRxiv - Microbiology 2021Quote: ... Negative selection was performed using EasySep Human CD4+ T Cell Enrichment Kit (Stemcell Technologies) after Lymphoprep density gradient (Stemcell Technologies ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T-cells were isolated using a RosetteSep(tm) kit (Stemcell Technologies, Vancouver, BC) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were obtained using a negative CD4 magnetic selection kit (Stemcell Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequent isolation of CD3+ T cells using negative selection isolation kits (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... HIV-negative donors using the RosetteSep Human CD4 T cell enrichment kit (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using ImmunoCult™ Human CD3/CD28 T Cell Activator antibodies (StemCell Technologies).
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bioRxiv - Immunology 2021Quote: ... CD3+ T cells or CD4+ T cells were isolated via Lymphoprep and RosetteSep (both STEMCELL Technologies). For Treg isolation ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were purified by negative selection using RosetteSepTM Human T cell enrichment cocktail (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Viable T cells were enriched using the Easy Sep T cell isolation Kit followed by the Easy Sep Dead Cell removal Kit (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies) or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... T cells were plated at 1 million cells/mL in either ImmunoCult XF T cell Expansion Medium (STEMCELL Technologies) supplemented with 200U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... the Negative Selection EasySep CD4+ T cell kit (17951) or RosetteSep Human CD4+ Cell Isolation Kit (15062) from Stemcell Technologies (Vancouver, Canada) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... CD8+ Tn or Teff cells isolated from LmOVA-infected mice by Thy1.1 positive selection kit (StemCell Technologies, Vancouver, BC, Canada) and were cultured immediately ex vivo in Seahorse XF medium (non-buffered DMEM 1640 containing 25mM glucose ...
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bioRxiv - Molecular Biology 2019Quote: ... CD4+ T-cells were isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2020Quote: Activation of CD4+ T-cell Targets: CD4+ T-cells were enriched by negative selection (Easysep, Stemcell Technologies), typically starting from 200×106 PBMCs per study participant ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were enriched for CD4+ cells using either the EasySep mouse CD4+ T cell enrichment kit (StemCell Technologies), or MojoSortTM CD4 T cell isolation kit (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified by negative selection using the EasySep™ CD4 enrichment kit (Stem cell Technologies), and further enriched for memory cells by depletion of naïve T cells using CD45RA beads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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bioRxiv - Immunology 2019Quote: ... Isolated T cells were cryopreserved at 2×107 T cells per vial in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies # 15061) or the EasySepTM Human Naïve CD4+ T cell Isolation Kit (Stem Cell Technologies #17953 ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated using the CD4+ Negative Selection Kit (StemCell Technologies, Cat# 19752) followed by fluorescence-activated cell sorting ...
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bioRxiv - Immunology 2021Quote: ... CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies, Vancouver, Canada) per manufacturer’s instructions and confirmed >90% pure by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were isolated from PBMCs using the EasySep positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Immunocult-XF T cell expansion media (Stem Cell Technologies) was supplemented with 1% penicillin/streptomycin/amphotericin B and optionally with 10% FBS ...
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bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells or CD56+CD3-NK cells were isolated from the PBMC population using the EasySep Human T Cell Isolation Kit or EasySep Human NK Cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 1-2 × 107 cells/mL and NK cells were frozen at 5 × 106 cells/mL in CryoStor CS10 (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies) at 1 × 106 cells/mL with 2 μL/mL ImmunoCult™ human CD3/CD28 T cell activator ...
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bioRxiv - Biophysics 2020Quote: ... ImmunoCult™ Human CD3/CD28/CD2 T cell activator (short T-Ac) (Stem cell Technologies, Vancouver, BC, Canada) was added to the cell culture medium (25 μL per 1 mL of cell suspension) ...
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bioRxiv - Immunology 2021Quote: ... T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies cat 10990) with 6.25μl per ml culture media at 2e6cells/ml ...
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by Lymphoprep and RosetteSep Human CD4+ T Cell Enrichment Cocktail (both STEMCELL Technologies). CD4-enriched cells were then CD25-enriched using CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies, 15023 and 15022) and EasySep Human CD4 T Cell Iso Kit (Stemcell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... coated tissue culture surfaces in mTeSR1 media (Stem Cell Technologies, 85850) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... coated tissue culture surfaces in mTeSR1 media (Stem Cell Technologies, 85850) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... T cells were extracted from spleen and CD4+ T cells were then purified using EasySep immunomagnetic negative selection (Stem Cell Technologies). Cells were then activated on plates as mentioned ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Microbiology 2019Quote: ... or the EasySep Human CD4+ T Cell Isolation Kit (STEMCELL Technologies, Vancouver, BC, Canada, Cat. #17952) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Monocytes were enriched from the same leukopoiesis products as T-cells using RosetteSep kits (Stemcell Technologies), and were then cultured at 1-2×106/ml in 12 well-plates with differentiation media containing 50 ng/ml Interleukin 4 (IL-4 ...
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bioRxiv - Immunology 2021Quote: ... T-cells were isolated with an EasySep CD3+ enrichment kit from StemCell Technologies (Vancouver, BC Canada), per the manufacturer’s protocol ...