Labshake search
Citations for Stemcell Technologies :
4951 - 5000 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... hPSCs were maintained and passaged in mTeSR1 medium (STEMCELL Technologies, Vancouver, BC, Canada) in cell culture plates coated with Matrigel (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T-cells were negatively isolated from PBMC by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies; average purity 93%, n=16).
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bioRxiv - Immunology 2022Quote: ... RBCs were lysed with ammonium chloride (STEMCELL Technologies). Trypan blue (Amresco)–negative cells were counted as live cells.
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bioRxiv - Immunology 2022Quote: ... Approximately 45mg of each frozen liver sample was placed in a 6-well tissue culture plate (Stem Cell Technologies) with 1 ml TST (2 mL of 2X ST buffer + 120 µL of 1% Tween-20 + 20 µL of 2% BSA brought up to 4 ml with nuclease-free water) ...
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bioRxiv - Immunology 2022Quote: ... NK cells isolated from buffy coats using an EasySep human NK cell enrichment kit (StemCell Technologies) were added to the mixed target population at a 1:1 effector:target ratio ...
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bioRxiv - Genomics 2022Quote: ... 6% v/v lymphoprep (STEMCELL Technologies, Canada), 1M betaine (#B0300-1VL ...
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bioRxiv - Immunology 2022Quote: Peripheral blood or the full contents of ABS:hematopoietic progenitor co-culture 24- wells were plated into complete methylcellulose media (Stem Cell Technologies, M3434). Colonies were scored 7-14 days after plating.
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bioRxiv - Developmental Biology 2022Quote: ... and passaged with Accutase (Stemcell Technologies). Mycoplasma testing was performed by PCR every 3 months ...
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bioRxiv - Genetics 2022Quote: ... Neutrophils were collected with EasySep Direct Human Neutrophil Isolation Kits (STEMCELL Technologies) or MACSxpress Neutrophil Isolation Kits human (Miltenyi Biotec ...
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bioRxiv - Genetics 2022Quote: Neural cells were induced using STEMdiff™ SMADi Neural Induction Kit (Stemcell Technologies, 08581) following monolayer cell culture protocol ...
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bioRxiv - Genetics 2022Quote: ... serum-free mTeSR plus medium (Stemcell Tech, 100-0276) according to manufacturer’s instructions and detached using 5mM EDTA/PBS or accutase ...
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bioRxiv - Genetics 2022Quote: ... and the differentiated neural progenitor cells were maintained in STEMdiff Neural Progenitor Medium (StemCell Technologies). All cells were grown at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of StemSpan serum-free expansion medium II(SFEM II; Stem Cell Technologies) with 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Human ESCs (wild type and ADAR1 KO, gifts from Dr. C. Rice, the Rockefeller University) were maintained in complete mTeSR1 (StemCell Technologies) and the differentiated neural progenitor cells were maintained in STEMdiff Neural Progenitor Medium (StemCell Technologies) ...
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bioRxiv - Genomics 2022Quote: ... rehydrated tissue was placed into a well of a 6-well plate (Stem Cell Technologies #38015) with 1 mL of ice-cold CST lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... blood samples were diluted with an equal amount of phosphate buffer saline solution (PBS) and slowly layered on top of the same volume of Lymphoprep density gradient medium (Stemcell Technologies Inc.). After that ...
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bioRxiv - Immunology 2022Quote: ... and 20 uL StemCell Heparin (07980, STEMCELL Technologies). After 21 days ...
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bioRxiv - Immunology 2022Quote: ... ALI medium components were as follows: ALI Complete Base Medium (05001, STEMCELL Technologies) containing 100uL of PneumaCult™-Ex Plus 50X Supplement ...
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bioRxiv - Genetics 2022Quote: ... the medium consisted of SFEM (Stem Cell Technologies) with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: Splenic naïve CD4+ T cells were negatively selected using an EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies). Naïve T cells were resuspended in supplemented RPMI 1640 medium with penicillin (100 U/ml) ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies, Canada). Cd4-/- mice were adoptively co-transferred intravenously (i.v. ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were negatively selected using an EasySep Mouse™ CD4+ T Cell Isolation Kit (Stem Cell Technologies). CD4+ T cells (4.0 × 105/well ...
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bioRxiv - Immunology 2022Quote: ... 50uL StemCell Hydrocortisone (07925, STEMCELL Technologies), and 20 uL StemCell Heparin (07980 ...
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bioRxiv - Immunology 2022Quote: ... whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies). The purity of the B cells used in these experiments was > 95% ...
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Immunology 2022Quote: ... The bone was thereafter finely cut and digested with 0.25% Collagenase I (Stem Cell Technologies) and DNAse I (25 Units/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... in mTeSR Plus medium (STEMCELL Technologies) and passaged by 5 min incubation at room temperature with 0.5 mM EDTA in PBS.
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bioRxiv - Genetics 2022Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated using Ficoll Paque Plus solution and density gradient centrifugation using SepMate tubes (StemCell Technologies). All samples were stored in vapor-phase liquid nitrogen after cryopreservation with 10% dimethyl sulfoxide until analysis ...
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bioRxiv - Genetics 2022Quote: ... Healthy donor bone marrow mononuclear cells were obtained from StemCell Technologies. Healthy adult CD34+ hematopoietic stem and progenitor cells (HSPCs ...
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bioRxiv - Developmental Biology 2022Quote: ... stem cell media is replaced with ameloblast base media consisted of either EpiCult-C media (StemCell Technologies, #05630) or RPMI 1640 Medium (Thermo ...
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bioRxiv - Developmental Biology 2022Quote: ... and cultured in mTeSR1 stem cell medium (StemCell Technologies, #85850) until cells reach confluency with medium changes daily ...
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bioRxiv - Developmental Biology 2022Quote: hiPSCs were cultured in mTESR Plus medium (Stemcell Technologies) on standard polystyrene plates coated with hESC-qualified Matrigel (Corning) ...
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bioRxiv - Immunology 2022Quote: ... or GZB-cre Zbtb20-fl/fl CD45.1 OT-I mice (KO) and naive CD8 T cells purified using EasySep Mouse Naive CD8 T Cell Isolation Kits (catalog no. 19858A; STEMCELL Technologies). Fifty thousand naive OT-I cells were retro-orbitally injected into congenic B6 recipient mice ...
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bioRxiv - Immunology 2022Quote: ... CD3+ cells were depleted using the negative selection cocktail RosetteSep™ (STEMCELL Technologies Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and naïve B cells were isolated by negative selection (StemCell Technology, Catalog #19254). B cells were seeded in 24 well flat-bottom plates at a density of 1 × 105 per well and stimulated in 1 ml of B cells complete medium ...
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bioRxiv - Immunology 2022Quote: ... contained within either a 50 mL SepMate™ (STEMCELL Technologies) or a 50 mL conical tube ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12 (Stemcell Technologies, Vancouver, Canada), IL-15 (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... A single-step density gradient centrifugation was performed to isolate peripheral blood mononuclear cells (PBMCs) by slowly layering diluted blood (30 mL) over 15 mL of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) contained within either a 50 mL SepMate™ (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... T cells from were then activated with Miltenyi MACS GMP T cell TransAct™ CD3/CD28 beads and seeded 1×106 T cells/ml in serum-free StemCell Immunocult™-XF media (Cat#10981, StemCell Technologies, Vancouver, Canada) with 20U/ml clinical grade human IL-2 (Novartis) ...
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
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bioRxiv - Microbiology 2022Quote: ... The sheared enteroids were then diluted to the appropriate concentration with IntestiCult™ Organoid Growth Medium (STEMCELL Technologies) supplemented with 10μM ROCK pathway inhibitor (Cayman chemicals) ...
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bioRxiv - Microbiology 2022Quote: ... and cultured with 500 μl/well murine IntestiCult™ Organoid Growth Medium (OGM, Stemcell Technologies, Cologne, Germany). Medium was changed every 2-3 days ...
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bioRxiv - Microbiology 2022Quote: ... plated in matrigel and cultured with 500 μl/well human IntestiCult™ OGM (Stemcell Technologies). Medium was changed every 2-3 days and organoids were passaged once a week ...
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bioRxiv - Microbiology 2022Quote: ... Loosely adherent immature BMDCs were collected and then CD11c+ DCs were enriched using CD11c positive selection kit (Stemcell Technologies, Cambridge, MA). Twenty-four hours after culture of purified subsets ...
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bioRxiv - Immunology 2022Quote: ... and incubated with NK cells isolated using the mouse NK cell isolation kit (STEMCELL Technologies cat #19855) from tumor-free wild type B6 mice in the indicated ratios in the presence of IL-2 as described previously (72) ...
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bioRxiv - Immunology 2022Quote: ... the tetramer-bound fraction was enriched via an adapted protocol of the EasySep PE Selection Kit II (StemCell Technologies, Inc.) developed by Marc Jenkins’ lab ...
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bioRxiv - Immunology 2022Quote: ... Gr1+ cells from MC38 tumors were isolated using the CD11b+ Gr1+ magnetic isolation kit (STEMCELL Technologies cat #19867) and incubated with NK cells isolated using the mouse NK cell isolation kit (STEMCELL Technologies cat #19855 ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from tumor-free wild type B6 mice using the mouse CD8 T cell isolation kit (STEMCELL Technologies cat #19853), labeled with Cell Trace Violet (Thermo Fisher cat #C34557 ...
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bioRxiv - Physiology 2022Quote: ... Selected cells (1 × 105 cells/ml) were expanded in StemSpan H3000 media (StemCell Technologies Inc) supplemented with StemSpam CC100 (StemCell Technologies Inc ...