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Citations for Stemcell Technologies :
151 - 200 of 9441 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Depending on the availability100 mm3-2000 mm3 cm tumor tissue piece was minced into smaller pieces and digested with 1x Collagenase & Hyaluronidase (# 7912, Stem Cell Technologies), or 1U/mL Dispase II (# D4693 ...
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bioRxiv - Cancer Biology 2024Quote: The EasySep™ Direct Human CTC Enrichment Kit (Catalog #19657, StemCell Technologies) along with the “Big Easy” Magnet (Catalog #18001 ...
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bioRxiv - Cancer Biology 2024Quote: ... was dispensed to a 50 mL SepMate™ (#85450, Stemcell Technologies,) tube ...
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bioRxiv - Cancer Biology 2024Quote: ... along with the “Big Easy” Magnet (Catalog #18001, StemCell Technologies) was used in this study to enrich cancer cells spiked into whole blood ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells were removed from single cell suspension using ACK lysis buffer and mononuclear cells were isolated by density gradient using SepMate Tubes (StemCell Technologies) and Lymphoprep density gradient media (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood mononuclear cells (PMBCs) were isolated from patient blood using Lymphoprep™ density gradient centrifugation following manufacturer protocols (STEMCELL Tech; Vancouver, Canada) and confirmed to contain ≥ 90% CD19+/CD5+ cells before use ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were enzymatically digested using Accutase (StemCell Technologies, Vancouver, BC, Canada Cat#07920) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... gliomaspheres were enzymatically digested using Accutase (StemCell Technologies, Vancouver, BC, Canada Cat#07920) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured at 37□°C in the presence of 5% CO2 in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Cancer Biology 2024Quote: ... spleen-derived B-cells were selected using the EasySep™ Mouse Pan-B Cell Isolation Kit (STEMCELL Tech).
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bioRxiv - Cancer Biology 2024Quote: Human whole leukocytes from anonymous healthy donors were isolated from apheresis cones from ARUP using RBC sedimentation with HetaSep (STEMCELL Technologies, Vancouver, British Columbia). Freshly isolated whole leukocytes were used fresh for in vitro culture.
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bioRxiv - Cancer Biology 2024Quote: ... and Lymphoprep density gradient media (StemCell Technologies). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: The RosetteSep™ Human CD45 Depletion Cocktail (Catalog #15112, StemCell Technologies) was used in this study to enrich cancer cells spiked into whole blood ...
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bioRxiv - Cell Biology 2024Quote: ... 25 μl accutase solution (Stemcell Technologies #07922) was added and left to incubate at room temperature for 10 min with gentle shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1% of heparin (StemCell Technologies), and of EGF ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Cancer Biology 2024Quote: ... single cell suspension was thawed into ImmunoCult-XF media (Stemcell Technologies) and incubated at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... in ImmunoCult-XF media (Stemcell Technologies, 10981), and expanded for 2-3 weeks until reaching the appropriate amount of T cells (usually >10 x 106 cells) ...
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bioRxiv - Cancer Biology 2024Quote: ... cultured cells were transformed into a hypoxic chamber (Stemcell Technologies; Cat# 27310) filled with a pre-mixed gas of 2% O2 ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in PBS (Hyclone, SH30256.01) supplementing with 0.005% DNase I (STEMCELL Technologies, 07900) at a concentration of 1×105 cells/μl ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were then treated with 2 ml pre-warmed Accutase (AT104, Stem Cell Technologies) and the vessel was incubated at 37ºC for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 0.5-1 x 107 cells/ml in “phase 1 expansion medium” consisting of Stemspan SFEM (Stem Cell Technologies) supplemented with Stem Cell Factor (SCF ...
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bioRxiv - Cell Biology 2024Quote: ... and 20% BIT (Stem Cell Technologies). The SCF was removed after day 3 of maturation and cells were cultured at 2X106 cells/ml from maturation day 4 to 7.
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bioRxiv - Cell Biology 2024Quote: ... were washed three times with PBS and incubated for 30 minutes at 37 °C with 60 ng/mL DNAse I (Stemcell technologies) in DNAse buffer solution (Table 1) ...
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Cell Biology 2024Quote: B cells were isolated from mouse spleens using EasySep Mouse B Cell Isolation Kit (Stem Cell Technologies 19854) and attached to poly-D-lysine coated coverslips at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: Hypoxic incubator chamber (Cat# 27310) and a single flow meter (Cat# 27311) were purchased from Stemcell Technologies. Pre-mixed gas of 2% O2 ...
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bioRxiv - Neuroscience 2024Quote: ... with mTeSR1 media (Stem Cell Technologies) and passaged using Accutase (Innovative Cell Technologies) ...
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bioRxiv - Cell Biology 2024Quote: FACS-sorted GFPhi MAPs from mammary glands of PdgfrαH2B-eGFP mice were seeded onto collagen-coated wells of a 24-well plate at 20,000 cells per well and cultured in DMEM media with 10% FBS and 10ng/ml bFGF (STEMCELL Technologies). Following 5 days of culture ...
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bioRxiv - Cell Biology 2024Quote: ... was added alone or with EGF (20 ng/ml; STEMCELL Technologies) or HB-EGF (20 ng/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... mSIOs were cryopreserved in CryoStor CS10 (Stem Cell Technologies, cat.# 07931) and stored in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... using StemFlex media supplemented with 10 μM ROCK inhibitor (Stemcell Technologies 72304). We cultured the cells to 75% confluence ...
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bioRxiv - Molecular Biology 2024Quote: ... Definitive hindgut endoderm was generated as reported.8 Induced cells were harvested and seeded on AggreWellTM400 Microwell Plate (StemCell Technologies Cambridge, MA) at 3.6 million cells in each well for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the remaining fresh murine BM cells were purified using human CD45 MicroBeads (Miltenyi Biotech) and cryopreserved in CryoStor® CS5 (STEMCELL Technologies, Cambridge, MA, USA). For secondary transplantation ...
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bioRxiv - Cell Biology 2024Quote: ... Each well contained 100 uL of StemSpan SFEM II medium (STEMCELL Technologies) supplemented with 1% penicillin-streptomycin (GIBCO) ...
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bioRxiv - Developmental Biology 2024Quote: ... 500µl of anti-adherence solution (STEMCELL Technologies) was added to each well ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was changed every second day and serial dilutions were performed in mTeSR+ medium containing Rock-inhibitor Y-27632 and CloneR (1:1000, Stem Cell Technologies #05889). Rock-inhibitor was removed after two days and the medium containing CloneR was changed every second day until colonies were ready to be picked ...
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bioRxiv - Molecular Biology 2024Quote: ... Pan-T cells were obtained from StemCell Technologies (Catalog: 70024).
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bioRxiv - Molecular Biology 2024Quote: ... HSPCs were obtained from StemCell Technologies (Catalog: 70060.1) and the Hematopoietic Cell Procurement and Resource Development Center at The Fred Hutchison Cancer Center ...
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bioRxiv - Molecular Biology 2024Quote: Airway cells: PneumaCult Ex Plus medium (StemCell Technologies #05040) was used for culturing ABCs ...
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bioRxiv - Molecular Biology 2024Quote: Primary T-cells were purchased from StemCell Technologies (#70024). Cells were cultured in X-Vivo 15 media supplemented with 5% human serum and 10 ng/mL of IL-2 ...
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bioRxiv - Molecular Biology 2024Quote: G-CSF Mobilized Human Peripheral Blood CD34+ Cells were purchased from StemCell Technologies (Catalog: 70060.1). Cells were thawed by resuspending in 10 mL of RPMI without FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Pneumacult Ex Plus (StemCell Technologies #05040), ROCK inhibitor 10 µM (Y-27632 ...
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bioRxiv - Molecular Biology 2024Quote: Human ABCs were cultured in PneumaCult Ex Plus medium (StemCell Technologies #05040) with addition of ROCK inhibitor 10 µM (Y-27632 ...
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bioRxiv - Molecular Biology 2024Quote: ... HSPC: StemSpan™ SFEM II supplemented with StemSpan™ CD34+ Expansion Supplement (10X) (StemCell Technologies, Catalog # 09720). IPSC ...
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bioRxiv - Cell Biology 2024Quote: ... BrainPhys media [Brainphys medium (StemCell Technologies, #05790) supplemented with NeuroCult SM1 (without vitamin A ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with NeuroCult SM1 (without vitamin A; StemCell Technologies, #05731) and N2 supplement-A (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... cells were cultured in BrainPhys Media (StemCell Technologies) with 20 ng/ml BDNF (PeproTech) ...