Labshake search
Citations for Stemcell Technologies :
101 - 150 of 8180 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Aliquoted CD34+ cell-enriched samples were cryopreserved in FBS (STEMCELL Technologies) containing 10% DMSO (Origen Biomedical) ...
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bioRxiv - Immunology 2024Quote: ... Positive selection of CD34+ cells was then performed on the low-density cells using the EasySep™ kit from STEMCELL Technologies) to obtain final CD34+ purities ...
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bioRxiv - Immunology 2024Quote: ... cells were rapidly thawed at 37 °C in a water bath and added dropwise into IMDM (STEMCELL Technologies) containing 10% FBS and 10 μg/mL DNase (MilliporeSigma) ...
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bioRxiv - Genomics 2024Quote: ... 3 x 106 cells were dissociated with Accutase (Stemcell Tech, 7920) and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). 24 h after transfection ...
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bioRxiv - Genomics 2024Quote: ... 24 x 106 SNRPN-2A-GFP VP64dCas9VP64 (maternal) or dCas9KRAB (paternal) iPSCs were dissociated using Accutase (Stemcell Tech, 7920) and transduced in suspension across five matrigel-coated 15-cm dishes in mTesR (Stemcell Tech 85850 ...
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bioRxiv - Genomics 2024Quote: ... 1.7 x 106 matSNRPN-2A-GFP Tet1cdCas9 or VP64dCas9VP64 iPSCs were dissociated using Accutase (Stemcell Tech, 7920) and transduced in suspension in a matrigel-coated 10-cm dish in mTesR (Stemcell Tech 85850 ...
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bioRxiv - Genomics 2024Quote: ... and transduced in suspension across five matrigel-coated 15-cm dishes in mTesR (Stemcell Tech 85850) supplemented with 10 μM Rock Inhibitor (Y-27632 ...
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bioRxiv - Genomics 2024Quote: ... and transduced in suspension in a matrigel-coated 10-cm dish in mTesR (Stemcell Tech 85850) supplemented with 10 μM Rock Inhibitor (Y-27632 ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼430-fold coverage of the gRNA library ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼580-fold coverage of the gRNA sub-library ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
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bioRxiv - Genomics 2024Quote: ... 250 µL of ImmunoCult Human CD3/CD28 T Cell Activator Mix (StemCell Technology) was added to the cells and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: ... Cells were stimulated with ImmunoCult Human CD3/CD28 T Cell Activator Mix (1:80; StemCell Technology) and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: CD3+ T cells were isolated from healthy donor leukopaks (Stemcell Technologies) using bead separation (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: PBMCs were isolated from HLA A2:01 specific healthy donor leukopaks (Stemcell Technologies) using a Stemcell isolation kit and cryopreserved ...
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bioRxiv - Genomics 2024Quote: ... Live tetramer positive cells were bulk sorted into CryoStor freezing medium (07959, STEMCELL Technologies) on a Sony SH800 sorter using a 100µm nozzle and frozen at –80 °C for single cell sequencing.
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bioRxiv - Genetics 2024Quote: Cryopreserved PBMCs were obtained from StemCell Technologies (Catalog # 70025). PBMCs were thawed and CD3+ T cells were isolated immediately by Pan T Cell Isolation Kit (Miltenyi # 130-096-535) ...
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bioRxiv - Genomics 2024Quote: ... CD3+ T cells were isolated by magnetic bead separation (StemCell Technology) from PBMCs of three leukopaks (Cambridge Biosciences ...
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bioRxiv - Genomics 2024Quote: ... Approximately 45 mg of flash-frozen skin or/fin was placed in a 6-well tissue culture plate (Stem Cell Technologies, cat. no. 38015) with 1 mL of TST buffer composed of 2 mL of 2X ST buffer ...
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bioRxiv - Genomics 2024Quote: ... using bead separation (Stemcell Technologies) and cryopreserved ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were isolated from pooled splenic suspensions by magnetic negative selection using EasySep Mouse CD4+ T Cell Isolation Kit (StemCell Technologies) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... cells were grown in Neurocult NS-A Proliferation Media (StemCell) supplemented with 0.0002% heparin (StemCell Technologies), EGF (20 ng/ml) ...
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bioRxiv - Genetics 2024Quote: ... coated plastic dishes and maintained in mTeSR1 medium (Stemcell Technologies, Canada, #05851), which was exchanged daily.
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells from healthy donors were purified from leukopaks purchased from Stem Cell Technologies using Ficoll EasySep Direct Human PBMC Isolation Kits (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human FGF10 (Stemcell Technologies) + 25 ng/ml human Noggin (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 10 vol% conditioned medium containing Rspondin1 (produced from HA-R-Spondin1-Fc HEK293T cell line ...
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bioRxiv - Immunology 2024Quote: ... + 25 ng/ml human Noggin (Stemcell Technologies) + 3 µM CHIR9902 (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... and 1 x CC100 (StemCell Technologies) consisting of cytokines SCF ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 20% BITS 9500 (StemCell Technologies), 2 mM l-glutamine (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... per manufacturer’s instructions (STEMCELL Technologies, Vancouver). Purity (typically >85% ...
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Microbiology 2024Quote: ... first ultracentrifugation was followed by a density gradient ultracentrifugation using OptiPrep (Iodixanol, Stemcell technologies, cat# 07820). OMV were resuspended in 50% OptiPrep and OptiPrep gradient (10% ...
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bioRxiv - Immunology 2024Quote: Esophageal biopsies were dissociated (27) and CD45+ cells were removed using positive isolation beads (STEMCELL TECHNOLOGIES). The untouched epithelial-enriched cell fraction was washed with PBS ...
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bioRxiv - Microbiology 2024Quote: ... and IntestiCult™ Organoid Growth Medium (OGM, StemCell Technologies), that was replaced every other day ...
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bioRxiv - Microbiology 2024Quote: ... Differentiation was triggered by Wnt3a removal with IntestiCult™ Organoid Differentiation Medium (ODM, StemCell Technologies) for 5 days with medium changes every other day.
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bioRxiv - Microbiology 2024Quote: ... 5 µM CHIR99021 (StemCell technologies) and 1.25% V/V DMSO) ...
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bioRxiv - Microbiology 2024Quote: ... and 10 µM Y-27632 (StemCell Technologies) and inoculated with HEV or mock infected ...
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bioRxiv - Microbiology 2024Quote: ... medium was replaced by ODM supplemented with 5 µM CHIR99021 (StemCell technologies) and 10 µM Y-27632 ...
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bioRxiv - Immunology 2024Quote: Splenic B cells were isolated by magnetic bead negative selection using EasySep mouse B cell isolation kit (Stem Cell Technology cat #19854). Cells were resuspended 2×106 cells/mL in media containing activating factors as described above and cultured at 200uL per well in 96 well flat bottom plates for CCK8 assay or round-bottom plates for CFSE dilution and antibody secretion assays ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic B cells were isolated by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies, #19854A) as specified by the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were isolated by EasySep™ Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies, 19258). All isolated T cells were activated by CD3/CD28 Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... cord blood mononuclear cells were ordered from STEMCELL Technologies (STEMCELL Technologies, 70007.1 or 70007.2). NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... cord blood mononuclear cells were ordered from STEMCELL Technologies (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies, 17955) and activated by 100 Gy irradiated K562 Clone 9 feeder cells ...
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bioRxiv - Immunology 2024Quote: Human pDC isolation was performed using an EasySep™ Human Plasmacytoid DC Enrichment kit (STEMCELL Technologies) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Natural Killer (NK) cells were isolated from Leukopaks (Stemcell Technologies) using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies) ...
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bioRxiv - Immunology 2024Quote: ... using EasySep™ Human NK Cell Isolation Kit (Stemcell Technologies). The isolated NK cells were incubated overnight at 37°C 5% CO2 in R10 (RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and subjected to a CD8+ T cell negative selection with the EasySepTM Mouse T cell isolation kit (STEMCELL Technologies cat#: 19853), according to the manufacturer’s protocol ...