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Citations for Stemcell Technologies :
51 - 100 of 9441 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... CD138 positive plasma cells were purified from available matching BM aspirates using immunomagnetic bead selection (EasySep™; Stem Cell Technologies). Total RNA from 7 of these patients was extracted from CD138+ cells using the miRNeasy Mini Kit (Qiagen GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... Then cells were seeded at 70% confluency on dishes pre-coated with Matrigel and cultured in StemMACS iPS-Brew XF + 5 μM Y-27632 (STEMCELL Technologies 72304). On D1 ...
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bioRxiv - Cell Biology 2024Quote: ... the media was replaced with Neural Induction Media (NIM) comprising the Neural Media (NM) + 0.5 μM LDN193189 (STEMCELL Technologies 72147) + 4.25 μM CHIR99021 (Miltenyi Biotec 130106539) ...
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bioRxiv - Cell Biology 2024Quote: ... All iPSC lines were nurtured in mTeSR Plus medium (STEMCELL Technologies). The iPSCs were differentiated into mesoderm-derived vascular smooth muscle cells (SMCs ...
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bioRxiv - Cell Biology 2024Quote: ... and c-Kit/CD117 antibodies (STEMCELL Technologies) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... iPSCs were maintained in mTeSR Plus (STEMCELL Technologies) under feeder-free conditions on hESC-qualified Matrigel (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... and EasySep Magnet (cat. no. #18000, StemCell Technologies) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... hiPSCs were maintained in mTeSR Plus (STEMCELL Technologies, 100-0276). For passaging ...
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bioRxiv - Developmental Biology 2024Quote: ... SSI and SSII media are parts of a 2-step suspension culture system with a base medium consisting of StemSpan Serum-Free Expansion Medium II (StemCell technologies), 2 mM of L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... coated dishes with mTeSR1 media (Stem Cell Technologies) and passaged every week by disassociating with 0.83 U/mL Dispase (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... and passaged every week by disassociating with 0.83 U/mL Dispase (Stem Cell Technologies). All experiments including hESCs were approved by Yale Embryonic Stem Cell Research Oversight (ESCRO).
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bioRxiv - Genetics 2024Quote: ... containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (cat.: 04444; cat: 04544.; STEMCELL Technologies, Vancouver, Canada). After 14 days ...
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bioRxiv - Developmental Biology 2024Quote: ... Reporter lines were screened for common karyotypic abnormalities using a qPCR kit (Stemcell Technologies) followed by verification via Thermo Fisher Cell ID + Karyostat and Pluritest services ...
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bioRxiv - Biophysics 2024Quote: ... ovaries were treated with collagenase (2 mg/mL; StemCell Technologies) and incubated in Modified Bath Saline (MBS ...
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bioRxiv - Bioengineering 2024Quote: ... Iscove’s Modified Dulbecco Medium (IMDM) containing 20% BIT 9500 serum substitute (STEMCELL Technologies), 1% 200 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... Freshly thawed CD34+-enriched HSCs isolated from BM (StemCell Technologies) were used as a positive control for engraftment (n=2 mice) ...
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bioRxiv - Bioengineering 2024Quote: ... Cultures were then maintained for 6 weeks in MyeloCult™ (StemCell Technologies, 05150) supplemented with 1x10-6 M hydrocortisone (StemCell Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 1x10-6 M hydrocortisone (StemCell Technologies, 74142), with half media exchanges twice per week.
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested and resuspended in MethoCult™ (StemCell Technologies, 04435) from LTC-IC assays after 6 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days by dissociating them using Accutase (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Cell Biology 2024Quote: ... in mTeSR Plus media (STEMCELL Technologies). The cells were kept at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Directed differentiation was performed using the STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... with base media consisting of Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 2% human blood plasma (STEMCELL Technologies, #70039), 3% human AB serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Medium was replaced on the basolateral side with PneumaCult medium (STEMCELL Technologies) which was prepared as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... HSPCs were thawed and cultured at a concentration of 5×105 cells/ml in the “HSPC medium” containing StemSpan (STEMCELL Technologies) supplemented with penicillin/streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... 250 nM StemRegenin1 (STEMCELL Technologies), 20 nM UM171 (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were cultured in StemSpan™ Serum-Free Expansion Medium version II (SFEM II; STEMCELL Technologies) containing 50 ng/mL each of recombinant human stem cell factor (SCF ...
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bioRxiv - Bioengineering 2024Quote: ... genomic DNA was extracted from primary isolated CD34+ cells (STEMCELL Technologies, Vancouver, BC, Canada) using a PureLink® Genomic DNA Minikit (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: Tumor spheres were assembled using AggreWell Microwell Plates (Stemcell Technologies). Briefly ...
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bioRxiv - Bioengineering 2024Quote: HSPCs were plated at a concentration of 1 × 103 cells/ml in a methylcellulose-containing medium (GFH4435, STEMCELL Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were passaged using Accutase (StemCell Technologies, 07920) and plated at a density of 100k cells/well ...
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bioRxiv - Bioengineering 2024Quote: ... medium from the upper chamber was removed and replaced with ATDC5 medium containing 0 or 0.1 ng/ml IL-1α (STEMCELL Technologies). Following two additional days of incubation ...
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bioRxiv - Bioengineering 2024Quote: ... using the EasySep™ Naïve CD4+ T cell isolation kit (STEMCELL Technologies) according to isolation procedures reported previously.10 Cells were cultured in serum-free AIM V media (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... ImmunoCult™-XF T Cell Expansion Medium (StemCell Technologies, Vancouver, Canada), CD3Ab OKT3 (Sino Biological ...
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bioRxiv - Bioengineering 2024Quote: ... Dissociation was performed by incubation in dissociation solution (1:1 dilution of 2.5% Trypsin (GibcoTM, Cat#: 15090046) and 1 U/ml Dispase (Stemcell Technologies, Cat#: 15569185)) for 10 min under gentle shaking and occasional gentle pipetting at 28°C ...
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bioRxiv - Bioengineering 2024Quote: ... coated dishes using mTeSRTM Plus medium (Stemcell Technologies; 100-0276). When healthy colonies of cells were formed ...
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bioRxiv - Bioengineering 2024Quote: ... we culture HBECs using PneumaCult™-Ex Plus Medium (STEMCELL Technologies, Cat. No. 05040). We passage HBECs using AccutaseTM Cell Detachment Solution (Innovative Cell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Passaging was with ReLeSR passaging reagent (Stem Cell Technologies). Due to their highly adhesive characteristics ...
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bioRxiv - Cancer Biology 2024Quote: Both WT and MT iPS cells were maintained in mTeSRTM1 medium with 1x Supplement media (Stem Cell Technologies, Catalog #85850) and supplemented with 10 μM Y27632 (Stemgent ...
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bioRxiv - Cancer Biology 2024Quote: ... mesoderm or ectoderm using the STEMdiff Trilineage Differentiation Kit Protocol (Stem Cell Technologies). After differentiation ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were suspended in CryoStor 10 (StemCell Technologies, Seattle, WA), aliquoted ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were isolated using the EasySep human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (StemCell Tech.) and cultured in X-Vivo15 ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
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bioRxiv - Bioengineering 2024Quote: ... 600000 single stem cells were seeded per well (500 cells/microwell) of AggreWell™400 (STEMCELL Technologies, Vancouver, Canada) plates in aggregation media with 50 μM Y27632 (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... 200-500 µm EBs were picked under a dissecting microscope and transferred onto adherent tissue culture plates (2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL2 (Stem Cell Tech 05270), with 0.5 % Protein free hybridoma medium (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... ROCK inhibitor (STEMCELL Technologies, 72304) and blebbistatin (Tocris ...
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bioRxiv - Bioengineering 2024Quote: ... We transition the cultures to ALI by removing the apical medium and replacing PneumaCult™- Ex Plus Medium with PneumaCult™-ALI Medium (STEMCELL Technologies, Cat. No. 05001). The medium is changed every other day ...
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bioRxiv - Bioengineering 2024Quote: ... at the density of 4.2×104 cells/well and add PneumaCult™-Ex Plus Medium (STEMCELL Technologies, Cat. No. 05040) to both the basal and apical chambers ...