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Citations for Stemcell Technologies :
1451 - 1500 of 1523 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... After nucleofection cells were plated onto a 10 cm dish coated with Synthemax (5 μg/cm2) with TeSR-E8 supplemented with CloneR (StemCell Technologies). Cells were cultured to 80% confluency ...
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bioRxiv - Neuroscience 2024Quote: ... and seeded on Geltrex-coated plates in STEMdiff Neural Induction Medium supplemented with SMADi and 10 nM Y-27632 (a ROCK inhibitor) (all reagents from STEMCELL Technologies). For further cultivation ...
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bioRxiv - Bioengineering 2024Quote: ... and reseeded as a single cell suspension at a density of 1.5×104 cells/cm in mTeSR™ Plus supplemented with 10 μM Y-27632 RHO/ROCK pathway inhibitor (72304, STEMCELL Technologies). Media was replaced with Y-27632 free mTeSR™ Plus after 24 hours.
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bioRxiv - Neuroscience 2024Quote: ... A 10 µM 2X working solution of CalbryteTM AM dye was prepared in sterile BrainPhysTM Imaging Optimized Medium (BPI, STEMCELL Technologies) containing 0.08% pluronic F-127 (#20050 ...
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bioRxiv - Cell Biology 2024Quote: ... IL-3/IL-6 and SCF cultures:3 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... For ECM concentration experiments and mechanical actuation experiments on Mechanoculture FX2 plates fibronectin coating (1 µg/mL and 10 µg/mL, in PBS StemCell Technologies) at 37°C for 1 hour were used.
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bioRxiv - Cell Biology 2024Quote: ... IL-11 and SCF cultures:2 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions harvested from cell culture or isolated from spleen or mediastinal lymph nodes were incubated with 10% Rat serum (STEMCELL Technologies), Fixable Viability Dye (ebioscience ...
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bioRxiv - Genetics 2019Quote: 1×10^5 cells from the RF of xenotransplanted mice were transferred to 1ml of MethoCult H4034 Optimum methylcellulose medium (StemCell Technologies, 04034) and plated onto a 35mm dish for human specific colony formation ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
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bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific, Waltham, MA] supplemented with 10% fetal bovine serum [Stemcell Technologies, Vancouver ...
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bioRxiv - Cancer Biology 2019Quote: Patient-derived PB and/or BM MNC were plated at 4 x 104 cells per well in 96-well plates in 200 µL of RPMI supplemented with 10% fetal bovine serum and 10X StemSpan (StemCell Technologies #02691) (Pardanani et al. ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were plated 48 hrs later at very low density (10 cells/cm2) on Laminin-521 with CloneR supplement (Stem Cell Technology) for clonal selection ...
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bioRxiv - Cell Biology 2019Quote: ... cells were harvested with GCDR and plated in Essential-E8 supplemented with 10 uM Rho-kinase inhibitor Y27632 (Stem Cell Technologies, 72304) on VTN coated 6-well culture dish to yield approximately 80% confluence next day ...
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bioRxiv - Neuroscience 2019Quote: ... 8 x 105 cells were resuspended in 100 μl Ingenio-solution (Mirrus) supplemented with Rock inhibitor (10 μM, Y-27632, Stem Cell Technologies) and 2.5 μg of CRISPR:hSpCas9 plasmid and 2.5 μg linearized DNA targeting plasmid and immediately pulse with program DC100 (using a 4D-Nucleofector ...
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bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated into 96 well flat bottom plates in growth medium supplemented with 10 µM of ROCK inhibitor (Stem Cell Technologies). Cells were plated at low density (2,500 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Neuroscience 2024Quote: ... Embryoid body media (EBM) was used for days 2 to 11 (Supplementary Table 2) and supplemented with 10 µM SB431542 (Stemcell Technologies, #72234), 0.1 µM LDN193189 (MedChemExpress ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested by centrifugation at 380 x g for 10 min at 4°C and resuspended in EasySepT Buffer (Stem Cell Technologies). HSPCs were purified by negative selection using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stem Cell Technologies) ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Alomone Labs), 10 ng/mL brain-derived neurotrophic factor (167450-02-STD, tebu-bio) and 500 µM dibutyryl-cAMP (Stemcell Technologies, 73886) and motoneurons were maintained for 25 DIV.
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Immunology 2023Quote: ... were then seeded overnight on mIL-1β capture antibody coated-coverslips in cRPMI medium supplemented with 10 ng/mL hrM-CSF (Stem Cell Technologies). 30 min before harvesting Mitotracker DeepRed (MTDR ...
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bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...
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bioRxiv - Bioengineering 2023Quote: ... Single cells were centrifuged at 200g for 5 minutes and resuspended in StemFlex supplemented with 10 µM Y-27632 (StemCell Technologies Inc.). We seeded 5.5×106 cells per 5.5 mL of mTeSR+ in each well of a six-well suspension plate ...
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bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were replated in mTeSR1 supplemented with 1% P/S and 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies, Vancouver, BC, Canada) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF iPSCs were plated in a T25 flask in the mTeSR1 medium with ROCK Inhibitor Y-27632 (10 µM, Stem cell Technologies, #72304). After 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were plated on Matrigel (In Vitro Technologies, cat. # FAL354277) coated plates in StemFlex medium supplemented with 10 μM ROCK inhibitor (StemCell Technologies, cat. #. 72304). Three days after plating ...
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bioRxiv - Cell Biology 2021Quote: ... YTS cells were cultured in RPMI media supplemented with 10% fetal bovine serum and NK92 cells were cultured in Myelocult (Stemcell Technologies, catalogue H5100) supplemented with 200 U/ml of IL-2 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Developmental Biology 2022Quote: ... and 105 cell/well were plated as small colonies (10-20 cell/colony) on Vitronectin-coated 12-well plates using mTeSR1 media (STEMCELL Technologies, Catalog# 12604013) supplemented with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in serum-free conditions in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753) supplemented with 10 ng/ml basic fibroblast growth factor and 20 ng/ml human epidermal growth factor.
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bioRxiv - Cancer Biology 2022Quote: H1 human ESCs were dissociated using accutase and plated as single cells in mTeSR™1 medium supplemented with ROCK inhibitor Y-27632 (10 μM, StemCell Technologies, Inc.) on matrigel to a final density of 20,000-30,000 cells per cm2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The epidermis was separated from the dermis by placing the strips in tubes containing 10 ml of 5U/ml Dispase II (Stem Cell Technologies, USA) and incubated at 4°C overnight and at 37°C for 2-3 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
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bioRxiv - Microbiology 2019Quote: CD34+ HPCs isolated from human bone marrow were infected at an MOI of 2 for 20h in IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies, Canada), 2 mM L-Glutamine ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...
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bioRxiv - Cancer Biology 2021Quote: Metastatic PDX tissues that had been frozen down in either FBS containing 10% dimethyl sulfoxide (DMSO) or primary PDX tissues that were frozen in neuronal stem cell medium (NSC medium, Stemcell technologies, Köln, Germany) containing 10% DMSO were thawed by brief incubation at 37°C and were transferred to basal NSC medium ...
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bioRxiv - Bioengineering 2022Quote: ... until a final volume of 200 ml and layered per 25 ml on top of 10 ml LymphoprepTM (07851, StemCell technologies, Köln, Germany) in 50 ml centrifugal tubes ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307, StemCell Technologies Inc.) followed by 72h incubation with daily media change ...
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bioRxiv - Cancer Biology 2024Quote: ... Corning 10-092-CV; 20% fetal bovine serum [FBS], Sigma-Aldrich F4135; and 0.1 mg ml-1 DNase I, Stem Cell Technologies 07900). Cell suspension was placed in a new tube ...
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bioRxiv - Neuroscience 2024Quote: ... media was replaced every other day with neuronal differentiation media prepared based on the Stem Cell Technology protocol: 10 ml of the BrainPhys™ Neuronal Medium (#05790; Stem Cell Technology) supplemented with 200 µl of NeuroCultTM SM1 Neuronal Supplement (#05711 ...