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Citations for Stemcell Technologies :
1101 - 1150 of 1523 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Cells were pre-treated with 10 µM Y-27632 (STEMCELL technologies) for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... plus 10 μM rock-inhibitor (Y-27632) (Stem Cell Technologies Canada) for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... 1% antibiotic-antimycotic supplemented with 10 ng/mL BDNF (StemCell Technologies), 10 ng/mL GDNF (StemCell Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% fetal bovine serum (FBS) (Stemcell Technologies, Vancouver, Canada) and 1X antibiotic/antimycotic solution (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and 10 µM all-trans retinoic acid (ATRA, Stemcell Technologies, 72264) (Differentiation Medium 1) ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were suspended in CryoStor 10 (StemCell Technologies, Seattle, WA), aliquoted ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). 24 h after transfection ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼430-fold coverage of the gRNA library ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10 μM Rock Inhibitor (Y-27632, Stemcell Tech, 72304). Cells were transduced at a MOI of 0.2 to obtain one gRNA per cell and ∼580-fold coverage of the gRNA sub-library ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 ng/mL recombinant murine SCF (STEMCELL Technologies; cat. #78064) for 7 days ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with 1:10 Hank’s buffer (STEMCELL Technologies 37150) supplemented with 10 µM Y-27632 (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... and 10 μM of rock inhibitor (Y-27632, STEMCELL Technologies, 72304) was added for the first 24 hours after seeding ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM ROCK inhibitor (Y-27632; STEMCELL Technologies, Catalog no. 72302) was added to replated cultures to help with adherence and to prevent differentiation ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... 2% BSA (Miltenyi) and 10 uM ROCK inhibitor (StemCell technologies 72302). For all wash steps ...
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bioRxiv - Cancer Biology 2022Quote: ... 1x B27 (Themofisher Scientific) and 10 µM Y-27632(StemCell Technologies). The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... EGF (20 ng/mL) and bFGF (10 ng/mL) (StemCell Technologies). All lines were cultured at 37°C and 5% carbon dioxide ...
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bioRxiv - Cell Biology 2024Quote: ... with 10 µM Rock Inhibitor (Stem Cell Technologies. Catalog No. 72304). The resuspended cells (normally > 500k cells ...
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bioRxiv - Neuroscience 2024Quote: ... EBs were treated with 10 μM SB431542 (ref.72234, Stemcell Technologies) from day 0 to day 7 and with 100 nM LDN193189 (ref.72147 ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from human bone marrow aspirates or PBMC derived CECs culture using EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
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bioRxiv - Immunology 2019Quote: Total CD8+ T cells were isolated from the peripheral blood of 2 healthy donors using EasySep™ Human CD8+ T Cell Enrichment Kit (StemCell Technologies). Isolated CD8+T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 beads (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF human iPSCs were seeded in a Matrigel-coated T25 flask in mTeSR1 medium (Stem Cell Technologies, #85870) with ROCK inhibitor ...
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bioRxiv - Bioengineering 2021Quote: ... CFU-E and CFU-GEMM Colony identification was performed 14 days after seeding in MethoCult media according to the Atlas of Human Hematopoietic Colonies (StemCell Technologies, 28700).
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coat (Research Blood Components) using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2021Quote: CD138+ myeloma cells were isolated from patient peripheral blood using a magnetic bead-based approach (EasySep Human CD138 Positive Selection Kit, StemCell Technologies 17877). The CD138+-enriched sample was titrated into 2.5e7 ...
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bioRxiv - Immunology 2022Quote: ... the Negative Selection EasySep CD4+ T cell kit (17951) or RosetteSep Human CD4+ Cell Isolation Kit (15062) from Stemcell Technologies (Vancouver, Canada) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The crypts were embedded in 30 μl of Matrigel and cultured with Human IntestiCult™ Organoid Growth Medium (OGM) (Basal Medium and Organoid Supplement, Stemcell Technologies) supplemented with 100 IU Penicillin and 100 μg/ml Streptomycin (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... mobilized peripheral blood mononuclear cells (PBMCs) and cord blood) using magnetic beads according to manufacturer’s protocol (EasySepTM Human CD3 Positive Selection Kit II, StemCell Technologies, Vancouver, Canada)
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bioRxiv - Cancer Biology 2022Quote: Differentiated human and murine H3K27M glioma cells were generated by dissociating the respective gliomaspheres into single cells with Accutase (Stemcell Technologies; # 07920) and subsequently cultured and maintained in the respective base media supplemented with 10% FBS for 14 days to generate a monolayer adherent culture ...
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bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... CD138+ primary myeloma cells were purified from fresh bone marrow aspirates of multiple myeloma patients using EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: CD4 T cells were isolated from PBMC by negative magnetic selection using the EasySep Human CD4 T Cell Enrichment Kit (StemCell Technology, 19052). Purity was typically >98%.
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bioRxiv - Immunology 2022Quote: ... Primary human CD8+ were isolated from healthy donor blood with commercially available negative selection kits (RosetteSep Human CD8+ T cell Enrichment Cocktail, STEMCELL technologies, UK) following the manufacturers instruction ...
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bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
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bioRxiv - Bioengineering 2019Quote: ... EGFP-transfected) cells were cultured on laminin-coated tissue culture flask in NeuroCult™ NS-A proliferation media for human (STEMCELL Technologies). Culture media was changed every two days ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then enriched for B cells by using the EasySep Human Pan-B cell Enrichment Kit (Stemcell Technologies, Vancouver, BC, Canada). PBMCs were incubated with an antibody cocktail containing CD2 ...
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bioRxiv - Immunology 2020Quote: ... Primary human airway epithelial cells isolated via bronchial brushings from consented healthy individuals were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...