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Citations for Stemcell Technologies :
1251 - 1300 of 1523 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... with or without 10 ng/mL ROCK inhibitor Y-27632 (STEMCELL Technologies, 72302). The condition which promoted the best viability and propagation was then applied to primary SSC cultures for 3 passages at 37°C and 5% CO2 and 2 freeze-thaw cycles ...
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bioRxiv - Bioengineering 2020Quote: ... the hiPSCs were treated with 10 μM ROCK inhibitor Y-27632 (STEMCELL Technologies). The cells were washed using Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Molecular Biology 2022Quote: ... About 10 million hPSCs were dissociated into single cells with Accutase (Stemcell Technologies), filtered with a 40 μm cell strainer (Corning) ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10 μM ROCK inhibitor Y-27632 (RI; 05990, STEMCELL Technologies, Canada) for the first 24 h after thawing or passaging ...
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bioRxiv - Cancer Biology 2022Quote: ... DMEM with 10% FBS and 1 mg/ml collagenase IV (STEMCELL Technologies, 07427) for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... pre-warmed media containing 10 ng/ml beta-interferon (STEMCELL Technologies, Cat #78113) was added to cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Single-cell passaging medium by adding Y-27632 (10 μM, Stem Cell Technologies) to mTeSR1 was used for the 1st day of feeder-free culture (Watanabe et al. ...
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bioRxiv - Immunology 2021Quote: ... counted and treated with 10 U mL−1 DNase I (STEMCELL Technologies, #07900) for 15 min at RT to prevent cell clumping ...
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bioRxiv - Immunology 2024Quote: ... and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed) (STEMCELL Technologies) for 1 hour on ice ...
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bioRxiv - Genomics 2024Quote: ... A specialized medium was created by mixing 10% CloneR™ (StemCell Technologies #05888) and 10 ng/l Pifithrin-α in mTeSR™ medium ...
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bioRxiv - Cell Biology 2023Quote: ... and resuspended in media supplemented with 10$M ROCK inhibitor Y27632 (STEMCELL Technologies). Cells were then seeded onto Geltrex-coated plates (12-well ...
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bioRxiv - Immunology 2023Quote: ... moDCs were treated with 10 μM RALDH inhibitor diethylaminobenzaldehyde (DEAB) (Stem Cell Technologies), 10 μM of PPARγ inhibitor (GW9662) ...
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bioRxiv - Cancer Biology 2023Quote: ... DMEM with 10% FBS and 1 mg/ml collagenase IV (STEMCELL Technologies, 07427) for 1 h ...
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bioRxiv - Genomics 2023Quote: H9 cells were treated with 10 uM Y-27632 (Stem Cell Technologies, 72304) for at least 2 h prior to nucleofection ...
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bioRxiv - Neuroscience 2023Quote: ... 3 × 10^6 iPSCs were seeded into an Aggrewell 800 well (STEMCELL Technologies) to form embryoid bodies (EBs) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% FBS and 1:10 dilution of collagenase/hyaluronidase (STEMCELL Technologies) at 37°C for 3 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1:10 dilution of 1 mg/ml DNase I (STEMCELL Technologies), triturated vigorously for 2 min ...
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bioRxiv - Biophysics 2023Quote: ... cleaned coverslips were incubated with 10 μg/mL of fibronectin (Stemcell Technologies, 07159)) at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 cm dishes were coated with Anti-adherence rinsing solution (Stemcell technologies, 7010) during 2 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 ng/ml basic fibroblast growth factor (bFGF) (STEMCELL Technologies; Cat #78003).
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bioRxiv - Developmental Biology 2023Quote: ... immersed in mTeSR1 media with 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies) to prevent dissociation- induced apoptosis and transferred to an incubator at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/ml basic fibroblast growth factor (bFGF) (STEMCELL Technologies; Cat #78003). For S7 cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged ∼1:10 at 80% confluency using 0.5mM EDTA (STEMCELL Technologies) and small cell clusters (50–200 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml SCF and 10 ng/ml Flt3-L (Stem Cell Technologies). 1 x 106 transduced HSPCs were resuspended in PBS and injected into the tail vein of 6-week-old female Balb/c mice ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... and Valproic Acid (VPA, 10 μM final concentration; #72292, Stem Cell Technologies, Canada) to enrich organoids for stem cells.82 After approximately 5 – 7 days ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were passaged 1:10 every 3 days using ReLeSR (StemCell Technologies #05873). Cells were maintained in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2024Quote: ... in Essential 8 Medium (Thermo) with 10 μM Y-27632 compound (StemCell Technologies). The media was replaced with Essential 8 Medium the following day and cultured until cells reached ∼90% confluence ...
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bioRxiv - Microbiology 2024Quote: ... in medium supplemented with 10 μM Rho kinase inhibitor (Y-27632; STEMCELL Technologies) at 1.35×106 cells/cm2 to achieve 95% confluence after 48h culture (Differentiation Day 0) ...
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bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Isolated PBMCs were used to isolate CD4+ T-cells by negative selection using the EasySep ™ Human CD4+ T-cells isolation kit (Stem Cell Technologies, Canada). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Neutrophils were freshly isolated directly from whole blood by negative selection immunomagnetic purification with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies; cat. no. 19666).
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bioRxiv - Immunology 2020Quote: ... were depleted that CD4+ T cells were negative selection and CD25+ cells were positive selection using the Easy Sep™ Human CD4+CD25+ T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada). We confirmed the removal of about 90 % of T-regs by FCM (S5 ...
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bioRxiv - Immunology 2021Quote: ... CD34+ peripheral stem cells were isolated from peripheral blood and expanded using the Complete Kit for Human Whole Blood CD34+ Cells (Stem Cell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Human whole leukocytes from anonymous healthy donors were isolated from apheresis cones from ARUP using RBC sedimentation with HetaSep (STEMCELL Technologies, Vancouver, British Columbia). Freshly isolated whole leukocytes were used fresh for in vitro culture.
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bioRxiv - Cell Biology 2024Quote: ... we performed a Cut and Run/Q-PCR (C&R/Q-PCR) experiment of 5×105 untreated cells and 5×105 cells treated for 30 minutes with recombinant human INFγ (STEMCELL Technologies, Vancouver, BC, Canada). For the C&R procedure ...
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bioRxiv - Systems Biology 2024Quote: ... mouse NGN2 at the AAVS1 locus and pC13N-dCas9-BFP- KRAB at human CLYBL intragenic safe harbor locus (between exons 2 and 3) were cultured in mTeSR Medium (Stemcell Technologies; Cat. No. 85850) on Corning Tissue Culture Treated Dishes (VWR International LLC ...
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bioRxiv - Microbiology 2024Quote: ... Monolayer cultures were seeded into 96-well plates using a 1:1 ratio of IntestiCult™ Organoid Growth Medium Human Basal Medium (StemCell Technologies, 100-0190) and Organoid Supplement (StemCell Technologies ...
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bioRxiv - Genomics 2024Quote: ... followed by buffer exchange and B cell selection using the EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (cat # 17963) from Stemcell Technologies (Vancouver, BC). Following B cell selection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were isolated from healthy donor-derived PBMCs using the EasySep™ Human CD8+ T Cell Isolation Kit (STEMCELL Technologies, Cat. # 17953). CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Cancer Biology 2024Quote: Primary human CD4 and CD8 T cells were isolated from PBMCs by negative selection using STEMCELL EasySep isolation kits (Stemcell Technologies, 17952 and 17953), and mixed 1:1 ...
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bioRxiv - Biochemistry 2024Quote: ... PBMCs were isolated from the blood of healthy volunteer and enriched for CD3+ Tcells using EasySep™ human T-cell isolation kit (Stemcell Technologies, Vancouver,Canada) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: CD4+ T cells were enriched from PBMCs by negative MACS selection using the EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies SARL, Grenoble, France), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: Flow cytometer-sorted murine or human HSCs were resuspended in 4 mL complete MethoCult GF M3434 medium or MethoCult™ H4435 Enriched (STEMCELL Technologies, 03434 and 04435) thawed at RT and 1 mL of the suspension was placed into 35 mm dishes in triplicates ...
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bioRxiv - Immunology 2022Quote: ... successfully transduced cells were enriched based on CD19t expression on day 11-15 using EasySep™ Human CD19 Positive Selection Kit II (Stemcell Technologies, Inc., Cat#17854), and the resulting CAR+ T cells were cryopreserved at day 15 or 17 ...
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bioRxiv - Immunology 2021Quote: ... Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies, Cat#: 19157 or 19159), or isolated from purified CD4+ or CD8+ T cells by CD45RO microbeads (see Suppl ...
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bioRxiv - Immunology 2021Quote: Untouched total T or isolated CD4+ or CD8+ T cells were purified from buffy coats or whole blood with RosetteSep Human T or CD4+ or CD8+ T cell enrichment cocktails (STEMCELL Technologies, Cat#: 15061, 15062, 15063). Memory CD4+ or CD8+ T cells were either isolated from PBMCs by EasySep Human Memory CD4+ or CD8+ T cell enrichment kit (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... the remaining fresh murine BM cells were purified using human CD45 MicroBeads (Miltenyi Biotech) and cryopreserved in CryoStor® CS5 (STEMCELL Technologies, Cambridge, MA, USA). For secondary transplantation ...