Labshake search
Citations for Stemcell Technologies :
1201 - 1250 of 1523 citations for IL 10 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Cells were passaged ∼1:10 at 80% confluency using ReLeSR (STEMCELL Technologies) and small cell clusters (50–200 cells ...
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bioRxiv - Genetics 2019Quote: ... at a final concentration of 10 μM and CHIR99021 (StemCell Technologies #72052) at a final concentration of 3,3 μM ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged ∼1:10 at 80% confluency using ReLeSR (STEMCELL Technologies) and small cell clusters (50–200 cells ...
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bioRxiv - Bioengineering 2022Quote: ... and cultured in NIM supplemented with 10 μM Y-27683 (Stemcell Technologies). On DIV8 ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng/mL of FGF-10 (all from Stem Cell Technologies), and HAO medium was replaced every 3–4 days.
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bioRxiv - Cell Biology 2024Quote: ... using StemFlex media supplemented with 10 μM ROCK inhibitor (Stemcell Technologies 72304). We cultured the cells to 75% confluence ...
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bioRxiv - Developmental Biology 2023Quote: ... and 10 µM ROCK-Inhibitor (Catalog no. Y-27632, Stem Cell Technologies). Organoids were maintained by hard splitting every week ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS (Cytiva, USA) and DNase I (Stemcell Technology, Canada) (1 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were cultured in StemFlexTM medium supplemented with CloneR (10×) (STEMCELL Technologies) and antibiotic-antimycotic (100× ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies cat #72304). Plates were coated with Gibco Geltrex Growth Factor Reduced Basement Membrane (Thermo catalog # A1413202) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FBS (Cytiva, USA) and DNase I (Stemcell Technology, Canada) (1 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... in mTeSR1 medium containing 10 μM Y-27632 ROCK inhibitor (STEMCELL Technologies) for the formation of embryoid bodies ...
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bioRxiv - Neuroscience 2024Quote: ... the SMAD inhibitor SB-431542 (SB, 10 µM, cat #72234, StemCell Technologies), the BMP inhibitor Dorsomorphin (DM ...
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bioRxiv - Immunology 2024Quote: ... and exposed to the RHO/ROCK pathway inhibitor (10 μM, StemCell Technologies) for 24 hours in incubator at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... Human neutrophils were isolated from whole blood of healthy donors by immunomagnetic negative selection using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Isolation began from an initial blood volume of 0.5-1 ml ...
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bioRxiv - Bioengineering 2021Quote: Primary testicular cells were isolated from human testicular biopsies as previously described.[41] Testicular biopsies were transported to the lab on ice in Hypothermosol® FRS (STEMCELL Technologies, 07935) and processed within 1-2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Bioengineering 2021Quote: ... the apical channel medium was removed from the human Lung Airway Chip and the basal medium was replaced with PneumaCult-ALI medium (PneumaCult™-ALI Medium, StemCell Technologies, 05001) supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Cancer Biology 2020Quote: Clonogenic assay was realized with cells seeded into 35mm petri dish in semi-solid methylcellulose medium (Methocult™ M3231 for murin cells or Methocult™ H4230 for human cells, Stemcell Technologies). Cells were treated with the drugs for 72 hrs and centrifugated ...
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bioRxiv - Cell Biology 2020Quote: Primary human airway epithelial cells isolated via bronchial brushings from consented healthy or asthmatic subjects were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×107/ml of PBMCs were used for isolation of CD4+ CD127low CD25+ Regulatory T cells using the EasySep™ CD4+ CD127low CD25+ Human Regulatory T Cell Isolation Kit (STEMCELL Technologies, MA) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were first enriched for CD8 T cells using EasySep™ human total or naïve CD8 T Cell enrichment kits (STEMCELL Technologies, Vancouver, Canada). CD56− and CD56+ PBMC fractions were sorted by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Immunology 2020Quote: ... Primary T cells were isolated from peripheral blood of healthy donors by negative selection using RosetteSept™ Human T Cell Enrichment Cocktail (STEMCELL Technologies, 15061) and activated with Dynabeads® Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: Peripheral blood was collected from Saudi patients into an EDTA-containing blood collection tube and treated with RosetteSepTM Human Progenitor Cell Basic Pre-Enrichment antibody cocktail according to the manufacturer’s instructions (Stem Cell Technologies Catalog#15226). After PBMCs separation and isolation ...
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bioRxiv - Immunology 2022Quote: T cells were directly isolated from peripheral or cord blood using the RosetteSep Human T-cell Enrichment Cocktail according to the manufacturer’s protocol (Stem Cell Technologies cat#15061). T cells were cryopreserved in 90% fetal bovine serum+10% DMSO and stored in vapor phase liquid nitrogen following isolation ...
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2023Quote: ... Primary CD8+ and CD4+ T cells were isolated by negative selection through either RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) or RosetteSepTM Human CD4+ T Cell Enrichment Cocktail (StemCell technologies ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were immediately processed to isolate CD4+ T cells by negative selection using the EasySep Human CD4+ T Cell Isolation Cocktail (StemCell Technologies, Cat. #17952) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated from fresh or frozen PBMCs by negative selection using the EasySep™ human CD4+ T cell isolation kit according to the manufacturer’s instructions (Stem Cell Technology, USA), and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... B cells were purified from PBMC by negative selection using the StemSep™ Human B Cell Enrichment Kit (STEMCELL Technologies Inc., Vancouver, Canada) following the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Primary NK cells were isolated from PBMCs by immunomagnetic negative selection using EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies Canada, Inc.) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2023Quote: Mononuclear cells from healthy human whole blood and CLL patients were isolated by density centrifugation using SepMate-50 tubes (STEMCELL Technologies, Vancouver, Canada). In short ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... assay done by culturing 100-sorted cells LIN-CD34+ CD38- CD9+ or CD9- in to MethoCult™ H4434 with human cytokine (Stem Cell Technologies) and colony formation score at day 12-14 of incubation in 37°C.
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bioRxiv - Molecular Biology 2023Quote: The human ES cell-lines (WA17 and RUES2; WiCell Research Institute, Madison, WI) were maintained in mTeSR1 Plus medium (STEMCELL Technologies, Vancouver, Canada) on plates coated with Matrigel (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were isolated from frozen PBMC tubes using the EasySep™ Human CD4+ T Cell Isolation Kit (Stem Cell Technologies, #17952) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10-M RHO/ROCK pathway inhibitor Y-276932 (STEMCELL Technologies Cat# 72304) were used to dissociate iPSCs to single cells during passaging ...
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bioRxiv - Genomics 2020Quote: ... cells were treated with 10 μM ROCK Inhibitor (Y-27632) (Stem Cell Technologies) in Essential 8 (E8 ...