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Citations for Macherey-Nagel GmbH :
2201 - 2250 of 2266 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: Leaf material of soil grown plants was collected and homogenized in liquid nitrogen prior to extraction of RNA with the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s advice ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... Correct clones were determined by sequencing and cultured for subsequent plasmid preparation using NucleoBond Xtra Midi/Maxi kits (Macherey-Nagel GmbH & Co. KG). For scaled CFPS reactions ...
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bioRxiv - Genomics 2022Quote: DNA extraction was performed from a single female 4th instar larva (ToLID ilPecGoss1) using the NucleoMag Tissue kit (Macherey-Nagel, Allentown, PA, USA). The high molecular weight DNA was prepared for PacBio sequencing using the SMRTbell Express Template Prep Kit 2.0 for Continuous Long Read (CLR ...
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bioRxiv - Microbiology 2022Quote: ... The total genomic DNA of the insect individual and its symbionts was extracted using the NucleoSpin Tissue XS Kit (MACHEREY-NAGEL, Duren, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cat#M0491S/L) and gel extractions were done with the Macherey-Nagel NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Cat#740609). DNA assemblies were carried out by In-Fusion Cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 x 109 cells were used for extraction of genomic DNA using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). PCR-free libraries (microbial short insert libraries ...
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bioRxiv - Plant Biology 2022Quote: ... the rhizosphere and root subpools were purified on an agarose gel (amplicon ∼450 bp) using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), whereas all other subpools were purified with CleanNGS beads ...
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bioRxiv - Bioengineering 2022Quote: ... 8×104 661W cells were seeded in a 24-well culture plate with DMEM and transfected with the plasmids containing guides using Lipofectamine™ Cells were harvested 72 hours following the transfection and Genomic DNA was then extracted using NucleoSpin® Tissue kit (Macherey-Nagel) following the manufacturer’s recommendation.
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bioRxiv - Bioengineering 2022Quote: Cultured cells were lysed in Macherey-Nagel RA1 buffer containing 1% β-mercaptoethanol and total RNAs were isolated using NucleoSpin RNA isolation kit (Macherey-Nagel 740955). Reverse transcription and PCR amplification were performed as described before21 ...
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bioRxiv - Plant Biology 2023Quote: ... and the resultant powder was transferred and redissolved in lysis buffer provided by the Nucleospin Plant II kit (Macherey-Nagel, Clonetech, CA, USA). Genome isolation was performed according to the manufacturer’s instructions ...
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Chromosome-level assembly of Cucumis sativus cv. ‘Tokiwa’ as a reference genome of Japanese cucumberbioRxiv - Genomics 2024Quote: We also extracted RNA from the leaves for gene annotation used by NucleoSpin RNA Plant kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany).
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bioRxiv - Genomics 2024Quote: The RNA from 3 independent replicates (100mg/replicate) per regime were extracted using the Macherey-Nagel Mini kit for RNA purification (Macherey-Nagel, Allentown, PA). Sample purity was assessed using the NanoDrop ND-1000 Spectrophotometer (NanoDrop ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from the interphase of extracted cells as described before [20] by using the NucleoSpin® RNA kit (MACHEREY-NAGEL, 740955.50). Isolated RNA was converted to cDNA by using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™ ...
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bioRxiv - Microbiology 2024Quote: Total DNA was extracted from baseline and post-conditioning soil sub-samples using the DNA Set for NucleoBond RNA Soil Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... Purified DNA for the Vitis vinifera high-coverage sample and Actinidia melanandra were extracted using the Nucleobond High Molecular Weight DNA kit (Macherey-Nagel, Düren, Germany). Nuclei isolation was performed prior to DNA extraction for the Actinidia sample using the PacBio protocol (Pacific Biosciences ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 50 mg of frozen and fine ground rosette leaf material from four biological replicates per genotype and growth condition using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products (with approximately 500-600 bp in length) were then purified with NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, MN) or with NZYGelpure kit (NZYtech ...
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bioRxiv - Genetics 2024Quote: ... the supernatant was removed and the remaining RNA pellet was dissolved in MQ water and further purified and DNAse treated using the Nucleospin RNA Clean-up Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and small (<200 nt) and large RNA (>200 nt) were extracted in separate fractions using the NucleoSpin® miRNA kit (Macherey-Nagel; Düren, Germany) following the manufacturer’s recommended protocols ...
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bioRxiv - Microbiology 2022Quote: ... A sample of 100 mg of the ground powder (or as much as was available) was subject to DNA extraction using a NucleoSpin™ DNA Stool Kit (Macherey-Nagel, Oensingen, Switzerland). We found only one sample of overwintered leaves hanging in a tree ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissue using a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers suggested by the stock facility (forward ...
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bioRxiv - Systems Biology 2020Quote: ... with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Plasmids were constructed from PCR-generated fragments and pET16b vector cut with restriction enzymes using the isothermal DNA assembly method (48) ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissues with a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers (forward 5’ to 3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from 100 freshly harvested seeds in three biological replicates using the NucleoSpin® RNA Plant and Fungi kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from a total of 24 samples of male brain (four vgll3*EE and four vgll3*LL individuals for each of the three time points) using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The collected samples were transferred to tubes containing 1.4 mm ceramic beads from Omni International ...
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bioRxiv - Microbiology 2023Quote: ... inoculated seeds and seedlings of the SynCom conditions (control, 6-strain, 8-strain, 12-strain SynComs) with the NucleoSpin® 96 Food kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions.
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bioRxiv - Zoology 2024Quote: ... Genomic (g)DNA was extracted and purified using the NucleoSpin®Tissue kit following the manufacturer’s instructions (Macherey-Nagel Gmbh & Co. KG, Düren, Germany). Primers used for the detection of all pathogens are found in Supplementary Data 3.
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bioRxiv - Cell Biology 2024Quote: ... RNAlater was removed then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenised with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Synthetic Biology 2023Quote: ... Base-editing efficiency when targeting a plasmid-borne gene was estimated by isolating plasmid DNA upon 24-h editing treatment by using the NucleoSpin™ plasmid EasyPure kit (Macherey-Nagel, Dueren, Germany). Either the isolated plasmids or the purified PCR products from independent samples were Sanger-sequenced using the Mix2Seq™ kit (Eurofins Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The pellet was washed once with sterile H2O and then each library was purified over two columns of the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). A total of 17 Mio were harvested ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and small (<200 nucleotides) and large (>200 nucleotides) RNA were extracted separately for each tissue with the NucleoSpin® miRNA kit (Macherey-Nagel; Düren, Germany). RNA quality and quantity were determined using a NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from tobacco leaf disks sampled from T1 plants and quickly frozen in liquid nitrogen using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized using 1 µg of total RNA according to the protocol in the RevertAid Reverse Transcriptase kit (Fermentas ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and bands of the correct size were excised and cleaned up with a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Cat. No. 740609.5) followed by cleanup with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2024Quote: ... tissues were immediately frozen in liquid nitrogen and stored at -80°C prior to total RNA isolation with NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel Inc., PA, US). RNA libraries for RNA-Seq were prepared using the Illumina TruSeq Stranded Total RNA Ribo-Zero Plant kit (Illumina ...
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bioRxiv - Genetics 2024Quote: ... using Ion Xpress™ Plus Fragment Library Kit (Thermo Scientifc, USA) and NucleoMag® NGS Clean-up and Size Select beads (Macherey-Nagel, Germany) following manufacturers’ instructions ...
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bioRxiv - Genetics 2024Quote: ... PCRs for loci of interest were run using Amplicon-EZ (Genewiz) partial IlluminaÒ adapters and amplicons were processed using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Catalog no. 740609.250). Paired end (2 x 250 bp ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA extractions were either performed with a modified CTAB 2%/PVPP 2% protocol (see Jolly et al. 2003) or the NucleoSpin®Tissue 96 kit (Macherey-Nagel, Karlsruhe, Germany).
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bioRxiv - Microbiology 2024Quote: The PCR product was run on a 0.8% agarose gel and a fragment of the correct size was excised for clean up using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Ref. No. 740609.5) followed by Ampure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were isolated and purified by the Gel clean up guidelines of the NucleoSpin Gel and PCR clean-up Kit (Macherey-Nagel, Ref. No. 740609.50).
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bioRxiv - Plant Biology 2024Quote: ... Maize leaf discs sampled at 4 dpi were homogenized using metal beads in a Retsch mixer mill and fungal genomic DNA was extracted with NucleoSpin® Plant II Kit (Macherey-Nagel, Dueren, Germany) as explained before ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gel purifications were performed using the Freeze N Squeeze DNA Gel Extraction Spin Columns (BIO-RAD, CAT#: 4106139 and NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL, CAT#: 740609.250).
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bioRxiv - Cell Biology 2024Quote: ... then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenized with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...
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bioRxiv - Developmental Biology 2024Quote: ... dsDNA repair templates were amplified by PCR using primers with containing 5′ SP9 modifications (IDT) and purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.250) prior to injections ...