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Citations for Macherey-Nagel GmbH :
1801 - 1850 of 2266 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Unincorporated PCR primers and dNTPs were removed from the PCR products using the Nucleospin PCR clean-up kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from sorted E14.5 fetal liver hematopoietic progenitor cells (KL; CD45+/Lineage−/cKit+/Sca1−) using the NucleoSpin RNA Plus XS kit (Macherey-Nagel). The RNA-sequencing libraries were prepared using KAPA RNA Hyper Prep Kit with RiboErase ...
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bioRxiv - Genetics 2021Quote: ... Each tissue sample was sonicated for a few seconds with the buffer LBP1 from NucleoSpin RNA Plus kits (MACHEREY-NAGEL) and divided into two tubes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
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bioRxiv - Microbiology 2021Quote: ... as well as cKD TgISU1 (incubated with or without ATc at 1.5 μg/mL for 3 days) were extracted using Nucleospin RNA II Kit (Macherey-Nagel). The cDNAs were synthesized with 450 ng of total RNA per RT-PCR reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 16 h after transfection and total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2021Quote: ... cells were stimulated with 15% FBS for 45 min and total RNA was extracted using Nucleospin RNA II kit according to the manufacturer’s protocol (Macherey-Nagel). Five hundred micrograms of total RNA wasused for complementary DNA synthesis using Thermo Scientific RT–PCR kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... After 16 hours cells were lysed and total RNA was isolated using NucleoSpin® RNA Plus Mini Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted by using the Macherey-Nagel Nucleospin® RNA Clean-up XS kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... and purified directly from the PCR mix or from an agarose gel (Gel and PCR clean-up kit, Macherey-Nagel); the two fragments were fused together thanks to a primer-encoded 20-30bps overlap during a second PCR amplification round and the product cloned into the pEMG suicide vector [20] using EcoRI and BamHI ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were subjected to a final gel purification using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).
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bioRxiv - Genomics 2022Quote: ... Midipreps for all constructs were prepared at final concentration >1mg/mL using the NucleoBond Xtra Midi Plus kit (Macherey-Nagel).
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bioRxiv - Developmental Biology 2022Quote: Total RNA was prepared from 100 mg of 11-day-old seedlings with the NucleoSpin RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... PBS was removed by aspiration after spinning down and continued to RNA extraction with Nucleospin RNA kit (#740955.50, Macherey-Nagel) (incubated in the first buffer for 15 min after vortexing) ...
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bioRxiv - Microbiology 2020Quote: RNAs from mock infected or infected (MOI=0.001) cell culture supernatant were extracted using the Nucleospin Dx Virus RNA purification kit (Macherey-Nagel). Then qRT-PCR was performed in triplicate as described20 ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: DNA from monolayer cultures as well as from rafts was isolated using the NucleoSpin Blood QuickPure kit (740569.250; Macherey-Nagel) according to the manufacturer’s instructions with some modifications for raft tissues ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were collected from the plate and plasmid pools were extracted using the NucleoBond Xtra Midi Kit (Macherey-Nagel) according to the manufacturer’s recommendation.
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bioRxiv - Immunology 2020Quote: ... Positive clones were identified by colony PCR and the plasmid was purified using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel). The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
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bioRxiv - Pathology 2021Quote: RNA was isolated from liver tissue using the NucleoSpin® RNA Plus Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bacterial DNA extraction was done after 48h of fermentation using Nucleospin DNA Stool Genomic DNA Purification Kit (Macherey-Nagel, Germany) and relative abundance of selected gut bacteria was determined by qRT-PCR.
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bioRxiv - Genetics 2021Quote: DNA extraction from fresh and scale connective tissues was performed using the NucleoSpin® Tissue Kit (Macherey-Nagel, Hoerdt, France), following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from frozen sections of breast tumor samples using the Nucleospin RNA set for Nucleozol kit according to the manufacturer’s protocol (Macherey-Nagel). Libraries were prepared using a modified version of the Takara SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian kit (Hendricks et al. ...
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bioRxiv - Neuroscience 2021Quote: Cell lysis and RNA extraction were performed on macrophage samples 24h post-activation using Nucleospin RNA extraction kit (Macherey-Nagel). Quality of RNA was confirmed on Agilent TapeStation (RINe>8) ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNAs have been extracted on an agarose gel and purified (using the PCR clean-up gel extraction kit #740609.50, Macherey-Nagel) and the sanger sequencings have been performed by Eurofins ...
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bioRxiv - Microbiology 2021Quote: ... and lysed using LBP lysis buffer for RNA extraction following manufacturer’s instructions (NucleoSpin® RNA plus extraction kit, Macherey-Nagel). Reverse transcription was then performed on 10 μL of RNA using High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Genetics 2020Quote: ... Obtained libraries were pooled in equimolar amounts and purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), followed by a second purification step of gel extraction and size exclusion with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... all isolates were grown overnight at 20 °C in liquid Zobell medium and bacterial DNA was extracted using the Nucleospin tissue kit following the manufacturer’s instructions (Macherey-Nagel).
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA of all mcr-positive isolates was extracted using NucleoSpin® Tissue kit (Macherey-Nagel GmbH & Co, Duren, Germany). The libraries were prepared using Nextera XT DNA Sample Preparation Kit and sequenced on MiSeq or NovaSeq 6000 platform (Illumina ...
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bioRxiv - Neuroscience 2023Quote: We generated high-quality DNA of the expression constructs for electroporations using the NucleoBond Xtra Midi kit (Macherey-Nagel 740410.50)
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were purified using the NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, catalog no. 740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was extracted from the heads of 30 male flies using the NucleoZol one phase RNA purification kit (Macherey-Nagel). Library preparation and sequencing was performed by BGI (Wuhan ...
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bioRxiv - Cell Biology 2023Quote: Dissected thyroids were immediately placed in RNAlater solution and total RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel) according to the manufacturer’ s instructions ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted from larvae or pupae of the selected lines using NucleoSpin Tissue gDNA extraction kit (Macherey-Nagel).
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bioRxiv - Bioengineering 2023Quote: We extracted RNA from live cells for bulk RNA-sequencing using the NucleoSpin totalRNA FFPE micro kit (Macherey-Nagel #740969.50) ...
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bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The 140-bp UPTAG and DNTAG libraries were purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel), quantified using the Qubit dsDNA HS kit (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from the final pellets using the NucleoBond High Molecular Weight DNA Kit according to the manufacturer’s instruction (Macherey-Nagel).
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 200 mg of stool sample using a modified NucleoSpin® DNA Stool kit (Macherey-Nagel, Germany) via physical ...
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bioRxiv - Genomics 2023Quote: Total RNAs were extracted from blood of 137 ewes with the Nucleospin® RNA Blood Kit (Macherey-Nagel, #Ref 740200.50) according to the manufacturer’s protocol starting with 800µL of whole blood with a DNAseI digestion treatment to eliminate contaminating genomic DNA ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from 100μl of the homogenate of crushed ticks using the NucleoSpin RNA kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 6-days-old seedlings grown in sterile culture using the RNA NucleoSpin Plant kit (Macherey-Nagel) according to the manufacturer’s instructions with on-column DNase treatment ...
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bioRxiv - Plant Biology 2024Quote: DNA-free RNA was isolated with NucleoSpin® RNA Plant and Fungi Kit as recommended by the manufacturer (MACHEREY-NAGEL) and used as template for reverse transcription as previously described (Lemaire-Chamley et al. ...
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bioRxiv - Cell Biology 2024Quote: ... A large amount of these plasmid vectors was prepared using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplicons were subjected to agarose gel size selection using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Amplicons were cloned into BsmBIv2-digested (New England BioLabs ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
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bioRxiv - Physiology 2023Quote: ... We prepared a large amount of these plasmid vectors using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA5/FRT-SUGCT and pOG44 plasmid DNA was purified by NucleoBond Xtra Midi Plus EF DNA purification Kit (Macherey-Nagel). Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen ...