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Citations for Macherey-Nagel GmbH :
1751 - 1800 of 2266 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: RNA isolation for multiple tissues was achieved by using the NucleoZOL one phase RNA purification kit (Macherey-Nagel #740404.200) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All constructs were verified by sequencing and purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Total RNA extraction and complementary DNA (cDNA) synthesis were performed using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany) and High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from TGF-β1-treated cells (as described above) using NucleoSpin® RNA kit (Macherey-Nagel) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: All expression constructs generated in this work were then midi-prepped using NucleoBond Xtra Midi kit (Macherey-Nagel 740410.50).
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bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
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bioRxiv - Genetics 2023Quote: ... except for genomic DNA for whole-genome sequencing that was extracted using NucleoSpin DNA purification kit (MACHEREY-NAGEL®).
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bioRxiv - Cancer Biology 2023Quote: RNA from sorted blasts or ex vivo-cultured cells was isolated using NucleoSpin RNA XS kit (#740902 Macherey-Nagel) or RNeasy Mini Kit (#74104 Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then lysed in Buffer RA1 and RNA was extracted with NucleoSpin RNA kit (Macherey-Nagel ref 740902.250). Total RNAs were then retrotranscribed to cDNA using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent ref 200436 ...
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bioRxiv - Immunology 2023Quote: ... The excised DNA fragments were further refined using nucleospin gel and by using PCR clean-up kit (Macherey-Nagel) and quantified for determining the purity and concentration ...
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bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from 50µm cryostat sections of left ventricular tissue samples with NucleoMag Tissue Kit (Macherey-Nagel, Germany) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: DNA was extracted from 20 mg of dried leaf tissue using the NucleoSpin Plant II kit (Macherey-Nagel, Denmark). DNA was extracted according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Plant Biology 2023Quote: RNA was isolated from 6-days-old seedlings using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted genomic DNA from faeces using the NucleoSpin®Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted from various mouse organs using the NucleoSpin Tissue kit (Macherey-Nagel Inc., Allentown, PA, USA). The DNAs were used for species-specific PCR with primers against human α-satellite and mouse plakoglobin genomic regions [35] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting digested fragment was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, 740609.250). Guide RNAs were designed using the CRISPOR tool (http://crispor.gi.ucsc.edu/ ...
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bioRxiv - Microbiology 2024Quote: Total RNA from lung tissue cells for hamsters was isolated using NucleoSpin RNA Kit (740955.250, MACHEREY-NAGEL, Duren, Germany). cDNA libraries were prepared by KAPA mRNA HyperPrep Kit following the standard protocol ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was extracted from the pellet using the instructions provided by a commercial kit (Macherey-Nagel GmbH & Co.). Each reaction consisted of 500 nM of primer FWD and REV (Table S3 ...
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bioRxiv - Biochemistry 2024Quote: RNA from 10 mL yeast culture was isolated using a mini kit for RNA isolation (Macherey-NAGEL, REF 740933.50). Next ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from 8-10 pooled organoids per sample using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) following the manufacturer9s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The gRNA cassette was isolated by gel electrophoresis with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and the DNA was then amplified by PCR using primers with Illumina sequencing adapters ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated by agarose gel electrophoresis and purified from agarose gels using the NucleoSpin kit (Macherey-Nagel). Purified PCR products were subcloned into linearized plasmid vectors of interest using the In-fusion cloning system following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were isolated from the grounded whole seedling using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, Germany) using the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... Target bands were excised and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel GmbH & Co. KG). The purified DNA was subsequently sequenced using the Sanger method to verify the specificity of the amplified fragments.
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bioRxiv - Plant Biology 2024Quote: ... Poly(A) tail PCR amplicons were purified with a NucleoSpin Gel and PCR Clean-up kit (740609, Macherey-Nagel), separated on 1% (w/v ...
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bioRxiv - Genetics 2024Quote: PCR products from nematode specimens were purified using a NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Germany). Nucleotide sequences were determined by direct sequencing using a BigDye Terminator Kit ver ...
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bioRxiv - Immunology 2024Quote: Purification of total RNA from cell lysates was done using the Nucleospin™ RNA plus kit (Macherey-Nagel™) and reverse transcribed using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: All plasmids were propagated in NEB 10β cells and midi-prepped using a Nucleobond Xtra midi kit (Macherey-Nagel). They were ethanol precipitated before reconstituting in either nuclease free water or filter sterilised 5 mM phosphate/5 mM KCl injection buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Whole genomic DNA was extracted from muscle tissue or fin clips using commercial tissue kits (Qiagen and Macherey-Nagel). Extraction quality was checked on agarose gel for the presence of high molecular weight DNA ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from cultured cells using the NucleoSpin RNA kit for RNA purification (MACHEREY-NAGEL, Ref 22740955.250) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: The extracted RPE or retina tissue was sonicated with lysis buffer from NucleoSpin RNA Plus Kits (MACHEREY-NAGEL, 740,984.250). Then ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Dü ren, Germany) and send to Eurofins Genomics for Sanger sequencing of both strands ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from 100 mg of ripe fruits using the Nucleo-spin RNA Plant mini kit (Macherey-Nagel). Library preparation and Nanopore sequencing were performed at the Ecole normale superieure genomic core facility (Paris ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated as indicated for 72 hours prior to harvesting of RNA using the Nucleospin RNA kit (Macherey-Nagel). First-strand cDNA synthesis was performed using iScript Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: RNA was purified from cerebellum samples from Cockayne patients and age- and gender-matched healthy individuals by using the Nucleospin RNA isolation kit (catalog no. 740955.250; Macherey-Nagel) with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from total thymocytes and from sorted T-cell subsets from MCMV-infected and uninfected mice according to the manufacturer’s instructions using the NucleoSpin Blood QuickPure kit (MACHEREY-NAGEL), and stored at −20°C until further analysis.
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bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... treatment with 100µg/ml RNase A (37° for 15 minutes) and 50µg/ml proteinase K and purification using the PCR cleanup kit (Macherey-Nagel).
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin® RNA II kit according to the manufacturer's protocol (Macherey-Nagel). Sixty nanograms of total RNA were reverse transcribed by using the RevertAid H Minus First Strands cDNA Synthesis Kit with oligo(dT ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted using the NucleoSpin Plant II Mini kit according to the manufacturer’s instructions (Macherey-Nagel, Hoerdt, France) with the following exception for the lysis step ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted DNA from recently collected field and greenhouse samples using NucleoSpin Plant Mini Kit II (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sequenced using Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sent for Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Biochemistry 2020Quote: ... Genomic DNA was isolated from the pellet of trophozoite/schizont stage parasites using the Nucleospin® tissue kit (Macherey-Nagel) as instructed by the manufacturer.