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Citations for Macherey-Nagel GmbH :
701 - 750 of 804 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from cells at the indicated time points post stimulation and/or infection using the NucleoSpin RNA extraction kit (Macherey-Nagel) as directed by manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... the targeted one was purified from a gel using the NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL, Düren, Germany) or the Monarch® DNA Gel Extraction Kit (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from different tissues (leaf, xylem, phloem, and root) using a commercial RNA isolation kit (Macherey-Nagel, Germany). According to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were run on a 2% agarose gel and purified from gel using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The DNA concentration of the samples was measured and samples were diluted to 1 ng/μL in 10 μL ...
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bioRxiv - Plant Biology 2024Quote: Root exudates were collected from all cartridges of split and non-split-root plants grown in SPE tubes using an SPE vacuum manifold (Macherey-Nagel) connected to a vacuum pump (V-300 ...
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bioRxiv - Molecular Biology 2024Quote: The crudes of the coupling reactions were analyzed by RP-HPLC using an EC 250/4 Nucleodur 100-3 C18ec column from Macherey-Nagel. Buffers ...
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bioRxiv - Microbiology 2024Quote: ... followed by capillary sequencing to identify positive clones from which plasmid DNA was purified in preparation for transfections using a midi prep kit (Macherey-Nagel).
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from ∼20 mg of dry herbarium plant material using the NucleoSpinⓇ Plant II kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s manual with slight modifications ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from MCF7 cells was obtained with a Macherey-Nagel™ Total RNA Isolation kit (Macherey-Nagel, Germany, Cat # 740955.50) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Amplified DNA was excised and purified from a 1% agarose gel using the NucleoSpin Gel & PCR Clean-up Mini kit (Macherey-Nagel). The target vector (pCW57-GFP-P2A-MCS ...
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bioRxiv - Genomics 2024Quote: RNA from muscle and bone marrow samples collected in Brussels was extracted separately using the Nucleospin® RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... DNA expression plasmids were extracted and were purified from bacterial cells with NucleoBond® Extra Midi Plus kit (Macherey-Nagel, Germany) and they were stored at -20°C until their use for transient transfection into mammalian cells.
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bioRxiv - Cell Biology 2024Quote: ... The cloned gRNA + pSpCas9(BB)-2A-Puro V2.0 constructs were then isolated from the bacterial culture using a plasmid isolation kit (Macherey-Nagel, #740588.50). Following isolation ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Neuroscience 2022Quote: RNA from purified astrocytes or brain cell suspension after enzymatic digestion was extracted using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250), genomic DNA was eliminated and cDNA was synthetised (Thermo Fisher Scientific #K1672) ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid DNA was purified from the remaining cultures using a NucleoSpin Plasmid (NoLid) high-pure plasmid mini prep kit (cat. no. 740499.50, Macherey-Nagel, Bethlehem, PA) according to vendor instructions and quantified using the Qubit dsDNA BR Assay (cat ...
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bioRxiv - Microbiology 2021Quote: ... mitochondrial and nuclear genomes we extracted genomic DNA from Pb-GFP infected hepatocytes treated or not with infusion using a NuceolSpin Tissue kit (Macherey-Nagel, Germany) according to manufacturer’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... All performed thin layer chromatography (TLC) was done with silica gel on aluminum foils (60 Å pore diameter) obtained from Macherey-Nagel and visualized with ultraviolet light (λ = 254 and 365 nm) ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated with Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany) for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.
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bioRxiv - Microbiology 2022Quote: ... Viral genome equivalents (vge) were determined by qPCR of DNA isolated from encapsidated virions isolated with NucleoSpin Blood kit QuickPure (Macherey-Nagel; 740569.250).
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bioRxiv - Microbiology 2020Quote: ... lung left inferior lobe from hamsters were cut into pieces and lysed with RA1 lysis buffer provided with the NucleoSpin® RNA Plus kit (Macherey-nagel), RNA extraction was performed according the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: gDNA from 6 male and 5 female pupae (sexed by eye) was extracted using the NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany), pooled by sex and used as template for subsequent PCRs ...
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bioRxiv - Genetics 2022Quote: RNA extraction was performed from frozen tissue in culture plates (P6 well plate, Falcon®) by using the NucleoSpin RNA kit (Macherey-Nagel). RNA concentration was measured with NanoDrop1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Zoology 2020Quote: Approximately 50-100 mg of tissue (from the second pereopod) was used for DNA extraction using the NucleoSpin® Tissue Kit (Macherey-Nagel) following the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: Viral biodistribution analysis - Genomic DNA (gDNA) was extracted from the brain region of interest with the NucleoSpin®Tissue Kit (Macherey-Nagel) extraction kit and 100 ng of gDNA was used as a qPCR template ...
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bioRxiv - Microbiology 2020Quote: We extracted DNA from the biomass pellets stored at −20°C using a NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Deutschland), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... The amplified PCR product is then purified from gel using the “Nucleospin Gel and PCR Clean-up” kit (Macherey-Nagel, Reference: 740609.250).
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from 2 × 105 cells untreated or butyrate-conditioned BMDMs using the NucleoSpin RNA extraction kit (Macherey-Nagel, 740902.50) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... MuSCs from the gaskin muscles were collected by FACS and then subjected to extract RNA with NucleoSpin® RNA XS kit from Macherey-Nagel. 1 ng high-quality RNA per sample were reverse transcribed and amplified into cDNA following Smart-seq2 library preparation protocol (Picelli et al. ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA from the LAB strains was extracted using a NucleoBond AXG column and Buffer Set III (U0544A & U0603A, MACHEREY-NAGEL, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from mock- and KSHV-infected blood or lymphatic ECFCs using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). RNA was further concentrated and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Plant Biology 2022Quote: ... O23 and d44a genomic DNA was extracted from liquid-cultured aerial hyphae using the NucleoBond high-molecular-weight DNA kit (MACHEREY-NAGEL, Germany). The genomic DNA was processed through the short-read eliminator kit XL (Circulomics) ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... RNA bands according to the size of the 18s and 28s rRNA were cut from the agarose gel and extraction was performed using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel). The extraction was performed according to the manufactureŕs protocol for RNA extractions from agarose gels using the buffer NTC (Macherey Nagel).
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bioRxiv - Zoology 2024Quote: ... total RNA was extracted from the second pooled sample of 60 bees using a NucleoSpin®RNA kit (Macherey-Nagel Gmbh & Co.), cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... The messenger RNA was purified from the total RNA of the spleen and bone marrow cells of immunized rabbits using the NucleoTrap mRNA kit (Macherey-Nagel, 740655) and according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... six samples each containing 5 whole larvae or 5 larval dissected tissues (central neuronal systems or body carcasses) were disrupted in 350 μl lysis buffer from the NucleoSpin RNA kit (Macherey-Nagel, #740955) containing 1% beta-mercaptoethanol ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from microglia after CD11b microbead isolation from P7 neonates born to and nursed by WT and Slc2a5−/− dams using the NucleoSpin RNA kit (Macherey-Nagel, 740955.250). The QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from tissues including apical notches in 4-day-old gemmalings using NucleoSpin RNA Plant (Macherey-Nagel, Duren, Germany) according to manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: Genomic DNA of the 54 genotypes was extracted from the leaves using a mixed alkyltrimethylammonium bromide buffer and the kit NucleoMag Plant (Macherey-Nagel, Germany). DNA samples were quantified using ThermoFisher Scientific fluorometry Qubit ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from INS-1 832/13 cells 48h after transfection using the NucleoSpin extraction kit (Macherey-Nagel, Düren, Germany). The amount of isolated RNA was measured using the NanoDrop system ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from 8–12 mg of dried tissue using the NucleoSpin 96 Plant II kit (Macherey-Nagel GmbH & Co. KG) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the medium in the reservoirs was collected and the cells from the chambers were lysed with 350 µl Lysis Buffer RA1 (Macherey-Nagel, Germany) supplemented with 3.5 µl ß-mercaptoethanol (Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... 700ng of the plasmid library was isolated from the liquid culture using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Genomics 2023Quote: Perca fluviatilis gDNA samples from 35 males and 35 females were extracted with the NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA from CD11B+ cells was extracted using an RNA XS Plus extraction kit and that from brains using a NucleoSpin RNA extraction kit according to the manufacturers’ protocol (Macherey-Nagel®). Reverse transcription was performed using 350 ng (CD11B+ ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Neuroscience 2024Quote: ... restriction sites for three hours at 37C and the linearized vector was purified from an agarose gel and cleaned using Nucleospin columns (Macherey-Nagel 740609.50). The C1(1-29)-TurboID ...