Labshake search
Citations for Macherey-Nagel GmbH :
551 - 600 of 804 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and Agilent microarray analyses were performed from isolated total RNA using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250). Per reaction 200 ng RNA was amplified and Cy3-labeled using the LIRAK kit (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: RNA from nasal washings and organ samples was extracted using the NucleoMag® VET Kit (Macherey-Nagel, Düren, Germany) in combination with a Biosprint 96 platform (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood using NucleoSpin Blood Quick Pure kit (Macherey-Nagel, Duren, Germany) and was stored in the gene bank run by Azabu University (Kanagawa ...
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bioRxiv - Microbiology 2023Quote: ... Small RNA was isolated from AW-EVs or ML-EVs pellets using NucleoSpin® miRNA (MACHEREY-NAGEL, Duren, Germany). The small RNA library was prepared using NEBNext Small RNA Library Prep Set for Illumina (New England Biolaps ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from TGF-β1-treated cells (as described above) using NucleoSpin® RNA kit (Macherey-Nagel) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
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bioRxiv - Cancer Biology 2023Quote: RNA from sorted blasts or ex vivo-cultured cells was isolated using NucleoSpin RNA XS kit (#740902 Macherey-Nagel) or RNeasy Mini Kit (#74104 Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from 50µm cryostat sections of left ventricular tissue samples with NucleoMag Tissue Kit (Macherey-Nagel, Germany) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: DNA was extracted from 20 mg of dried leaf tissue using the NucleoSpin Plant II kit (Macherey-Nagel, Denmark). DNA was extracted according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Plant Biology 2023Quote: RNA was isolated from 6-days-old seedlings using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted genomic DNA from faeces using the NucleoSpin®Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted from various mouse organs using the NucleoSpin Tissue kit (Macherey-Nagel Inc., Allentown, PA, USA). The DNAs were used for species-specific PCR with primers against human α-satellite and mouse plakoglobin genomic regions [35] ...
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bioRxiv - Microbiology 2023Quote: The nucleic acids were extracted from the FFPE tissue sections using the Nucleospin total RNA FFPE XS (Macherey-Nagel). RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Microbiology 2024Quote: Total RNA from lung tissue cells for hamsters was isolated using NucleoSpin RNA Kit (740955.250, MACHEREY-NAGEL, Duren, Germany). cDNA libraries were prepared by KAPA mRNA HyperPrep Kit following the standard protocol ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was extracted from the pellet using the instructions provided by a commercial kit (Macherey-Nagel GmbH & Co.). Each reaction consisted of 500 nM of primer FWD and REV (Table S3 ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from 8-10 pooled organoids per sample using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) following the manufacturer9s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... lupulus was isolated from young leaves using the NucleoSpin Plant II (740770-50; Macherey-Nagel GmbH and Co. KG.), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated by agarose gel electrophoresis and purified from agarose gels using the NucleoSpin kit (Macherey-Nagel). Purified PCR products were subcloned into linearized plasmid vectors of interest using the In-fusion cloning system following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were isolated from the grounded whole seedling using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, Germany) using the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from both parents and W/M-bulks using the NucleoZol reagent (Macherey-Nagel, Düren, Germany) and purified with the Direct-zol RNA MiniPrep Plus Kit (Zymo Research ...
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bioRxiv - Genetics 2024Quote: PCR products from nematode specimens were purified using a NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Germany). Nucleotide sequences were determined by direct sequencing using a BigDye Terminator Kit ver ...
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bioRxiv - Immunology 2024Quote: Purification of total RNA from cell lysates was done using the Nucleospin™ RNA plus kit (Macherey-Nagel™) and reverse transcribed using SuperScript™ III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Whole genomic DNA was extracted from muscle tissue or fin clips using commercial tissue kits (Qiagen and Macherey-Nagel). Extraction quality was checked on agarose gel for the presence of high molecular weight DNA ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from cultured cells using the NucleoSpin RNA kit for RNA purification (MACHEREY-NAGEL, Ref 22740955.250) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: The extracted RPE or retina tissue was sonicated with lysis buffer from NucleoSpin RNA Plus Kits (MACHEREY-NAGEL, 740,984.250). Then ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from 100 mg of ripe fruits using the Nucleo-spin RNA Plant mini kit (Macherey-Nagel). Library preparation and Nanopore sequencing were performed at the Ecole normale superieure genomic core facility (Paris ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from total thymocytes and from sorted T-cell subsets from MCMV-infected and uninfected mice according to the manufacturer’s instructions using the NucleoSpin Blood QuickPure kit (MACHEREY-NAGEL), and stored at −20°C until further analysis.
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin® RNA II kit according to the manufacturer's protocol (Macherey-Nagel). Sixty nanograms of total RNA were reverse transcribed by using the RevertAid H Minus First Strands cDNA Synthesis Kit with oligo(dT ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted DNA from recently collected field and greenhouse samples using NucleoSpin Plant Mini Kit II (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from 1 ml of crude homogenate containing 6 μl of proteinase K (22 mg/ml, Macherey-Nagel), incubated overnight at 55°C.
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bioRxiv - Biochemistry 2020Quote: ... Genomic DNA was isolated from the pellet of trophozoite/schizont stage parasites using the Nucleospin® tissue kit (Macherey-Nagel) as instructed by the manufacturer.
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bioRxiv - Bioengineering 2021Quote: ... Unincorporated PCR primers and dNTPs were removed from the PCR products using the Nucleospin PCR clean-up kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Each tissue sample was sonicated for a few seconds with the buffer LBP1 from NucleoSpin RNA Plus kits (MACHEREY-NAGEL) and divided into two tubes ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was prepared from 100 mg of 11-day-old seedlings with the NucleoSpin RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were collected from the plate and plasmid pools were extracted using the NucleoBond Xtra Midi Kit (Macherey-Nagel) according to the manufacturer’s recommendation.
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bioRxiv - Pathology 2021Quote: RNA was isolated from liver tissue using the NucleoSpin® RNA Plus Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... His6-BsrA was purified from the cell lysate by chromatography on Ni-agarose columns (Protino Ni-TED 1000, Macherey-Nagel) with 300 mM imidazole in phosphate buffer used for elution ...
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bioRxiv - Genetics 2021Quote: DNA extraction from fresh and scale connective tissues was performed using the NucleoSpin® Tissue Kit (Macherey-Nagel, Hoerdt, France), following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... Formed [32P]ATP was separated from the remaining [32P]PPi by thin-layer chromatography (TLC) on polyethyleneimine plates (Macherey-Nagel) in 4 M urea and 750 mM KH2PO4 pH 3.5 ...
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bioRxiv - Biophysics 2022Quote: ... The product was then purified from 1% agarose gel and extracted using the NucleoSpin clean up kit (Macherey-Nagel, 740588.50).
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bioRxiv - Cell Biology 2022Quote: Total RNA from ΔbolA and control cell line was isolated independently six times using NucleoSpinTM RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from the rotten food waste with NucleoSpin DNA Stool kits (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and quality checked by using a NanoDrop (Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2023Quote: DNA extraction from dried filters was done using NucleoSpin™ Forensic Filter kit (Macherey-Nagel GmbH & Co .KG, Düren – Germany). Filter parts were folded and incubated in 600 μL of lysis buffer T1 at 56 °C for two hours with tube horizontally agitated and then centrifugated 1 min 30 sec at 11,000 g ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 6-days-old seedlings grown in sterile culture using the RNA NucleoSpin Plant kit (Macherey-Nagel) according to the manufacturer’s instructions with on-column DNase treatment ...