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Citations for Macherey-Nagel GmbH :
451 - 500 of 804 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Silica gel-coated aluminum backed thin-layer chromatography (TLC) plates were purchased from Macherey-Nagel (Oensingen, Switzerland). The tritium screens used for autoradiography were obtained from Fujifilm (Dielsdorf ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted from the bacterial strains using the NucleoSpin@ Microbial DNA kit (Macherey-Nagel, Germany). Whole-genome sequence analyses were performed at Bioengineering Lab ...
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bioRxiv - Cell Biology 2024Quote: Total cellular RNA was extracted from near-confluent cells in 12-well plates using NucleoZOL (Macherey-Nagel), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA from cells was isolated using NucleoSpin RNA Kit according to the manufacturer’s protocol (Macherey-Nagel). To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... The band was later eluted from the gel using NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel). The eluted product was cleaned using ExoSAP-IT™ (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from cells using the Macherey-Nagel Nucleospin RNA purification kit (catalogue#: 740955.50, Macherey-Nagel) following the manufacturers methods ...
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bioRxiv - Evolutionary Biology 2024Quote: Genomic DNA was extracted from individual frozen tissue samples using the NucleoSpin tissue kit (Macherey-Nagel®) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: RNA from cell culture supernatant was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative PCR (RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was collected from the DT40 cells using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co., Germany) and subjected to complementary DNA synthesis and quantitative PCR using a ReverTra Ace qPCR RT Kit (Toyobo Life Science ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA from individual embryos was isolated with the NucleoSpin® Tissue XS kit (Cat#740901, Macherey-Nagel). A PCR reaction was performed using the isolated genomic DNA with primers flanking the CAS9 target site ...
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bioRxiv - Developmental Biology 2020Quote: Total mRNA was extracted from pools of 30 embryos using NucleoSpin RNA kit (Macherey-Nagel, Bethlehem, PA, USA). Complementary DNA (cDNA ...
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bioRxiv - Genetics 2021Quote: Genomic DNA from 5 males was extracted using the NucleoSpin Tissue XS kit (Macherey-Nagel, ref #740 901). To amplify the Rsp satDNA region ...
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bioRxiv - Plant Biology 2020Quote: ... DNA recovered after ChIP and DNA from input chromatin was extracted by DNA isolation kit (Macherey-Nagel, Germany) and then analysed by qPCR ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from N/Tert or HFK cells using the NucleoSpin RNA extraction kit (Macherey-Nagel). RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted DNA from fresh spores stored at -20°C using the Nucleospin Soil Kit (Macherey-Nagel, Germany). Genomes were sequenced using Illumina 150 bp paired-end sequencing technology at 46X coverage on average ...
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bioRxiv - Microbiology 2021Quote: DNA and RNA extractions were performed using the RNA x DNA from soil kit (740143, Macherey-Nagel, Germany). The RNA sample was treated with Turbo DNA-free Kit (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from GYP treated GLUTag cells using the NucleoSpin® RNA kit (Macherey-Nagel, UK) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: mRNA from encysting and non-encysting KO strains was isolated by NucleoTrap mRNA Mini Column kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA was extracted using the NucleoSpin tissue mini kit for DNA from cells and tissue (Macherey-Nagel). DNA integrity was assessed by TapeStation (Agilent ...
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bioRxiv - Pathology 2022Quote: We extracted total RNA from 10 mg liver tissue using NucleoZOL reagent (Macherey-Nagel GmbH & Co. KG, Germany). First ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids from selected positive clones were extracted and purified on silica columns (NucleoSpin® Plasmid, Macherey-Nagel™), then sequenced with their specific oligonucleotides (Eurofins Genomics) ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA (gDNA) was isolated from sorted and unsorted cells using the Nucleospin Tissue Mini Kit (Macherey-Nagel) and sgRNA sequences were retrieved by a nested PCR approach that pre-amplified sgRNAs in a first round with primers specific to the lentiGuide-Puro construct (5’-AGAGGGCCTATTTCCCATGA-3’ ...
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bioRxiv - Immunology 2022Quote: ... The plasmid DNA was extracted from the bacterial cultures using NucleoBond™ Xtra Midi Plus (Macherey-Nagel, 740412.10) according to the manufacturer’s guidelines ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... Cells were washed with PBS once and RNA isolated from lysed cells Nucleospin RNA kit (#740955.50, Macherey-Nagel) according to manufacturer’s instructions including rDNAse treatment ...
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bioRxiv - Neuroscience 2021Quote: ... Endotoxin-free plasmid DNA was purified from clones with correct sequences (Nucleobond Xtra Maxi EF, Macherey-Nagel Inc.), and re-suspended in endotoxin-free water.
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from infected and non-infected HCT8 cells using NucleoSpin RNA Kit (Macherey-Nagel, Germany). An on-column DNase digestion with a RNase-free DNase was included in the process described by the fabricant to remove any genomic DNA contamination in RNA samples ...
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bioRxiv - Immunology 2022Quote: Total RNA from one hundred thousand TILs was extracted using a commercially available RNA isolation kit (Macherey-Nagel) following the manufacturer’s recommendations and ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products from the first reaction were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Germany) and used (1 µL ...
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bioRxiv - Genetics 2020Quote: ... the single band was purified from a gel with a NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and its concentration determined ...
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bioRxiv - Genetics 2020Quote: ... Samples were separated on a C18 column (EC 100/2 Nucleoshell RP-18, 2.7 μm, from Macherey-Nagel) using a gradient of 0.1% formic acid / isopropanol over 0.1% formic acid / water ...
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bioRxiv - Genetics 2020Quote: DNA was extracted from blood samples using the NucleoSpin Blood kit (Macherey-Nagel, GmbH & Co. KG, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified from agarose gels using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... The final plasmid library DNA was isolated from the culture using the Nucleobond Xtra Maxi kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was extracted from the samples using the NucleoSpin® DNA Food kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel, Hoerdt, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: To clone the NERCLIN cDNA we extracted total RNA from 143B cells using Mini spin kit (Macherey-Nagel). Then RNA was reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher scientific) ...
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bioRxiv - Genetics 2020Quote: ... RNA and protein were simultaneously extracted from pellets using the NucleoSpin TriPrep Kit (Macherey-Nagel, supplied by ThermoFisher). Deletion of the target region was confirmed by end-point of DNA using primers flanking the guides (primers 5’-AGGGGTTGAGCTGGATTCC-3’ and 5’-CCAGCACCCACTCTGTAGAT-3’ ...
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bioRxiv - Microbiology 2023Quote: ... Its DNA was extracted from mycelium and conidia with the NucleoBond High Molecular Weight DNA kit (Macherey-Nagel), with the mechanical disruption of about 30 mg of lyophilized mycelium with beads for 5 min at 30 Hz ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA were extracted from GBM control and EMP3 KO cells using the Nucleospin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: DNA samples was extracted from different salmon tissues using the NucleoSpin® Tissue kit (Macherey-Nagel, no. 740741) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from a single F1 mosquito using the NucleoSpin Tissue kit (Macherey-Nagel Inc., 740952.50). A diagnostic PCR was then performed to fully amplify the Act5C-ECFP marker using the primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total mRNA was extracted from leaf tissue with a NucleoSpin RNA/Protein kit (REF740933, Macherey-Nagel GmbH & Co.), then treated with DNase before transcription into cDNA using a Superscript III First-Strand Synthesis System (18080-051 ...
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bioRxiv - Microbiology 2023Quote: ... The gDNA was isolated from the transgenic parasite lines using the NucleoSpin Blood Kit (Macherey-Nagel; Dueren, DE) following the manufacturer’s protocol and used as template to verify the successful vector integration by diagnostic PCR (for primer sequence ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... total RNA after McIdas RNAi was isolated from U2OS cells using the Nucleospin RNA II kit (Macherey-Nagel). 1 μg RNA was converted to cDNA using M-MLV transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: Fosmid DNA of the hit clones was extracted with the NucleoBond Xtra Midi kit from Macherey-Nagel (France) following supplier recommendations ...
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bioRxiv - Immunology 2024Quote: Serum samples from all experimental groups were extracted using the NucleoSpin RNA isolation kit (Macherey-Nagel, Allentown, PA). Following viral RNA extraction ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction was performed using a combination protocol between the NucleoSpin® Plant II kit from Macherey-Nagel and the DNeasy plant mini kit from QIAGEN ...
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bioRxiv - Genomics 2023Quote: High molecular weight DNA was extracted from blood cells using a NucleoBond AXG column (Macherey-Nagel, Düren, Germany), which was followed by purification with phenol-chloroform ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from fresh bacterial cultures using the NucleoSpin® 96 Tissue Core Kit (Macherey-Nagel, Germany). All DNA samples were sequenced at the Max Planck-Genome-Centre Cologne using the Illumina paired-end technology with read lengths of 150 bp ...