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Citations for Macherey-Nagel GmbH :
401 - 450 of 804 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated from each sample using the NucleoSpin Plant RNA extraction kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions and in combination with Fruit-mate for RNA Purification solution (Takara Bio Europe SAS ...
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bioRxiv - Biochemistry 2022Quote: ... and viral RNA from cell lysate was extracted using the Nucleospin RNA plus mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA from 32 MPM cell lines was extracted with Nucleospin Blood kit (Macherey-Nagel, Düren, Germany) and 500 ng were hybridized to Affymetrix CytoScanHD Arrays (Affymetrix ...
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bioRxiv - Microbiology 2021Quote: ... The intracellular and extracellular RNA from cells was isolated using respective kits (MACHEREY-NAGEL GmbH & Co. KG) and the SARS-CoV-2 RNA was quantified using a commercial kit (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 5×106 B16F10 tumor cell lines using NucleoSpin® Tissue (MACHEREY-NAGEL), according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... BACs were extracted from bacterial culture using the NucleoBond® Xtra Midi plasmid extraction kit (Macherey-Nagel) according to the manufacturer’s provided instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from 24h treated samples using the NucleoSpin® RNA kit (Macherey-Nagel, UK) and cDNA was synthesised using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µl from each sample was spotted onto a POLYGRAM CEL 300 PEI-TLC plate (Macherey-Nagel), and developed with 0.5 M LiCl (Sigma) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we extracted DNA from liver tissue of a single mouse using a Nucleospin Tissue Kit (Macherey-Nagel) and quantified the DNA with a QuantiFluor dsDNA System (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Protino® Ni-NTA Agarose and NucleoBond® Midi Plasmid DNA Purification Kit were from Macherey-Nagel, Germany ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs from harvested cells were extracted using the NucleoSpin RNA extraction kit (Cat. # 740955.250, MACHEREY-NAGEL), and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat ...
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bioRxiv - Immunology 2021Quote: ... RNA from cultured ILC2s was extracted using a NucleoSpin RNA Kit according to manufacturer’s instructions (MACHEREY-NAGEL). The concentration and purity of extracted RNA was measured using a Spectrophotometer NanoDrop 1000 (Thermo Fisher Scientific) ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: RNA was isolated from MEFs or NIH3T3 cells using the Nucleospin RNA plus kit (Macherey-Nagel, 740984.5). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from whole blood samples using the NucleoSpin 96 RNA tissue kit protocol (Macherey-Nagel) with some modifications as previously described15.
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from cell culture fluids using the NucleoSpin RNA Virus Kit (Macherey-Nagel, Germany). Total RNA from cells was isolated using TRIreagent (Sigma ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from the infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel) and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies ...
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bioRxiv - Immunology 2021Quote: Total cellular RNA from IFN-treated hAEC cultures was extracted with the NucleoMag RNA kit (Macherey-Nagel) according to the manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Genomics 2021Quote: ... The resulting cultures were recovered from the LB-agar plates and a maxipreps were performed (Macherey-Nagel) to purify the plasmid DNA.
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from iPSCs and EBs using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) with the manufacturer’s instructions and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: Total RNA from cultured cells was extracted using the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, Germany). RNA was retrotranscribed using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA from thirty-three clones and WT tachyzoites extracted using NucleoSpin DNA RapidLyse kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Viral RNA from the supernatant was extracted using the NucleoSpin RNA Virus Isolation Kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse tissues using the Nucleospin RNA Plus kit (Macherey-Nagel, cat# 740984.50), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA from viral supernatants was isolated using Nucleospin Viral RNA isolation kit (Macherey-Nagel GmbH & Co. KG). qRT-PCR was carried out using nCOV-19 RT-PCR detection kit from Q-line Molecular to quantify SARS-CoV-2 RNA following manufacturer’s protocol on Roche LightCycler 480.
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 °C) and fragments from the supernatant were purified by PCR clean-up spin column (Macherey-Nagel).
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Small RNA was isolated from frozen and ground flower samples using a NucleoSpin miRNA kit (Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... The cssDNA released from the phage were then extracted with NucleoBond Xtra Midi EF kit (Macherey-Nagel) following the manufacturer’ s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and excised from a 0.8% agarose gel for purification using the NucleoSpin Gel Cleanup Kit (Macherey-Nagel). The insert pool and digested backbone were then ligated overnight at 16°C using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from sorted Nkp46+ or CD8+ cells with the NucleoSpin RNA XS kit (Macherey-Nagel) and cDNA was prepared using the SMARTer Ultra Low RNA kit (Clontech Laboratories ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from heart tissue was extracted with the NucleoSpin RNA Mini Kit with DNase (Macherey-Nagel). cDNA was synthesized using oligo dT with the Flex Script cDNA Synthesis kit (Quanta bio) ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted from cells corresponding to 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Molecular Biology 2023Quote: ... RNA of progeny virions was extracted from supernatants using the NucleoSpin RNA virus kit (Macherey-Nagel, #740956) according to the manufacturer’s instructions and stored at −80 °C until PCR-based analysis.
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was isolated from rosette leaves using the NucleoBond HMW DNA Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Large RNA above 200 nucleotides were isolated from macrophage samples using the NucleoSpin miRNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... around 20 mg was digested in 200 µL of lysis buffer (NucleoMag™ Tissue from Macherey-Nagel) and 25 µL of proteinase K at 56°C ...
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bioRxiv - Microbiology 2023Quote: ... and total RNAs were extracted from the samples using a NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). cDNAs were synthesized from the extracted RNAs using a PrimeScript Double Strand cDNA Synthesis Kit (Takara Bio ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from the isolated organs and conserved in 1ml NucleoProtect RNA (Macherey-Nagel, Germany). Samples were later processed for total RNA isolation with NucleoSpin RNA (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from the resulting pellets using the NucleoMag DNA Kit (Macherey-Nagel, Allentown, PA, USA) with the aid of the AutoPure96 robot for the purification step ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted from wild-type worms using the Nucleospin RNA® kit (Macherey-Nagel – 740955) or Trizol Reagent™ (Ambion® ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from liver pieces (30 mg) using a NucleoSpin kit (Macherey-Nagel cat# 740955.25) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from the primary CEC lines using a NucleoSpin RNA XS kit (Macherey-Nagel), in accordance with the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: RNA purification and reverse transcription were performed using the Nucleospin RNA kit from Macherey-Nagel (Düren, Germany) and the High capacity cDNA reverse transcription kit from Applied Biosystems (Woolston ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We extracted total genomic DNA from each whole individual sample using NucleoMag® Tissue Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: DNA samples extracted from mouse fecal pellets using the NucleoSpin Soil DNA Extraction Kit (Macherey-Nagel, Germany), underwent 16S rRNA gene amplicon sequencing ...
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bioRxiv - Microbiology 2024Quote: DNA plasmids were amplified from DH5α cultures and purified using endotoxin-free DNA extraction kits (Macherey-Nagel) as previously described (28) ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted from the bacterial strains using the NucleoSpin@ Microbial DNA kit (Macherey-Nagel, Germany). Whole-genome sequence analyses were performed at Bioengineering Lab ...
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bioRxiv - Pathology 2024Quote: ... total RNA was isolated from cultured muscle cells using NucleoSpin RNA Virus (Macherey-Nagel SAS, Hoerdt, France) and from pooled muscle sections of animal biopsies using TRIZOL-reagent (Life Technologies ...
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bioRxiv - Physiology 2024Quote: Total RNA from liver samples was extracted and purified using the Nucleospin RNA kit (Macherey-Nagel, 740955.250). RNA quality and quantity was assessed using Nano Drop 2000 (Thermo Fisher Scientific ...