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Citations for Bio-Rad :
201 - 250 of 3840 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Secondary antibodies used were either anti-rabbit (170-6515 Bio-Rad; 1:3000) or ECL anti-mouse (NA931 Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibodies used were: Goat Anti-mouse (ref: 1706516, BIORAD, 1:5000) or Goat Anti-rabbit (ref ...
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bioRxiv - Microbiology 2019Quote: ... Ssp6-HA was detected using anti-HA primary antibody (MRC PPU Reagents, University of Dundee, 1:6,000) and HRP-conjugated anti-mouse secondary (1:10,000; Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... HRP (horseradish peroxidase)-conjugated goat anti-mouse (#176516, 1:10,000 for WB) and anti-rabbit IgG antibodies (#176515, 1:10,000 for WB) were from Bio-Rad. Alexa Fluor conjugated goat anti-mouse (1:500 for IF) ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected with anti-rabbit (1:3,000) and anti-mouse (1:20,000) secondary antibodies coupled to horseradish peroxidase (Bio-Rad), diluted in 3% BSA-PBST ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Genomics 2022Quote: Antibodies and their working concentrations: anti-mouse (1:5000; #7076, Cell Signaling Technology, or 1:20000, #STAR117D800GA, Bio-Rad), anti-rabbit (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011 and 1:5,000 dilution, ref: 170-6515, Bio-Rad) in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed four times with binding buffer containing 0.1% Tween-20 and bound proteins were eluted with 2X Laemmli Sample Buffer (Bio-Rad Laboratories). Eluted proteins were subjected to electrophoresis on a 17% resolving gel at 120V for 2 hrs and transferred to PVDF membrane at 180mA for 2 hrs and immunoblotted with His-Probe-HRP (Pierce ...
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bioRxiv - Biochemistry 2021Quote: Primary screening for nanobody binding was achieved by a small-scale pulldown method in which Affi-gel 10 affinity resin (Bio-Rad) was bound to laforin and subsequently to each purified VHH clone34 ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Immunology 2022Quote: ... were amplified by PCR using 5’ and 3’ primers with overhangs containing T7 binding sites using iProof High-Fidelity Taq (Bio-Rad). PCR reaction products were run on an agarose gel to check for the correct amplicon ...
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bioRxiv - Biochemistry 2023Quote: ... The slurry was gently stirred for 3 h to allow binding and then loaded into a glass Econo-Column (Bio-Rad). The column was washed with 10 Column Volumes (CV ...
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bioRxiv - Biochemistry 2023Quote: ... to saturate the N-terminal metal binding site,10 prior to buffer exchange using a centrifugal buffere exchange device (Bio-Spin, Bio-Rad) into 200 mM ammonium acetate supplemented with 2 x CMC of C10E5 ...
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bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified mouse α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were washed three times for 5 min and incubated with either a 1:5000 (Mouse)/1:10000 (Rabbit) dilution of horseradish peroxidase-conjugated secondary antibodies or with fluorophore-conjugated 1:5000 dilution of Starbright700 (Biorad) for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2000 dilution of BioRad affinity purified goat α-rabbit or goat α-mouse IgG conjugated to alkaline phosphatase ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated secondary antibodies were diluted 1:10000 and 1:2000 for polyclonal Goat-α-Mouse (Bio-Rad, 170-6516) and Goat-α-Rabbit HRP (Dako ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were then treated with HRP-conjugated goat anti-moue secondary antibody (1:10000) diluted in blocking solution for 1 h and developed with enhanced chemiluminescent (ECL) substrate (Cat#1705060, Biorad).
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bioRxiv - Biochemistry 2020Quote: ... the membranes were incubated with goat anti-rabbit antibody conjugated to HRP (1:500 or 1:1000 against MEK or ERK/GST, respectively) (BioRad) for one hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... blots were incubated for 1-2 h at RT with an anti-rabbit HRP-conjugated secondary antibody (diluted 1 : 3,000, Bio-Rad) in TBST with 5% milk ...
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bioRxiv - Pathology 2019Quote: ... After incubation of the blots with HRP-conjugated secondary antibodies (1:3000 for goat anti-rabbit or 1:10,000 for goat anti-mouse, Bio-Rad), specific protein bands on the blots were visualized by applying enhanced chemiluminescence reagent Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... antibody at 4°C overnight and in goat anti-rabbit IgG horseradish peroxidase conjugated secondary (1:5000) antibody for 1 hour before detection using ECL detection (BioRad) and ChemiDoc Imaging (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... Corresponding mouse or rabbit horseradish peroxidase-conjugated secondary antibodies (diluted 1:10,000 in 1x TBST) were added for 1 hr at room temperature (Bio-Rad). Chemiluminescence was detected by adding Clarity Western ECL Substrate (1:1 of luminol and peroxide solutions ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for immunoblotting: Talin-1 (1:5000 dilution, Mouse monoclonal, Clone 97H6; Bio-Rad, Cat# MCA4770), YAP (1:1000 dilution ...
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bioRxiv - Immunology 2023Quote: ... and secondary antibodies (Anti-Rabbit or Anti-Mouse, CST, 1:5000 and 1:2000) and revealed using Clarity Western ECL (BioRad). Image J was used to quantify the intensity of western blot bands ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were washed in 0.1% TBS-Tween and incubated with the secondary antibody (1:10000) for 1 h at room temperature (RT) and revealed by chemiluminescence (Clarity ECL western blotting substrates, BioRad) using ImageQuant LAS 500 chemiluminescence CCD camera (GE Healthcare Bioscience AB) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with primary antibodies (mouse anti-human Aβ 82E1 [1:500, IBL 27725], rat anti-CD68 [1:250, BioRad MCA1957] ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Microbiology 2020Quote: ... incubated with goat α-rabbit 2° antibody conjugated to HRP (1:5000) (Bio-Rad) in TBS-T at room temperature (1 h) ...
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bioRxiv - Cell Biology 2020Quote: ... and hFAB Rhodamine Anti-Tubulin Antibody (1:2500, Bio-Rad, Cat# 12004165, RRID: AB_2884950) for 1 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... both with horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (1:10,000; Bio-Rad). Anti-OmpA antibody54 was used at 1:20,000 and a custom anti-TssL antibody (Eurogentec ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated 1h with HRP-conjugated secondary antibodies at 1:5,000 (Bio-Rad). After 4 more washes ...
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bioRxiv - Bioengineering 2022Quote: ... fibrosis was identified with an anti-collagen antibody (Bio-Rad, Hercules, CA, 1:500) with an Alexa Fluor™ 488 secondary (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies against CD68 (1:500, mouse-anti-rat, Bio-Rad/Serotec, MCA341R) and Iba1 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... 1:3,000 and secondary antibody goat anti-mouse IgG StarBright Blue 700 (Bio-Rad) 1:2500 ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad; 1:2000) and goat anti-rabbit IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... and monoclonal anti mouse secondary antibodies conjugated to horseradish peroxidase (1:5000, Bio-Rad). After incubation with ECL substrate (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... We immunopanned RGCs from retinal suspension using Thy1.2 antibody (CD90, 1:800, Bio-RAD) and 0.02% BSA (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... Secondary antibody was goat anti-mouse IgG horseradish peroxidase conjugate (1:5,000; Bio-Rad). Blots were developed using the Luminata Forte Western HRP substrate reagent (Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... Revelation was performed using corresponding antibody (Table 1) with ECL Clarity Max (Biorad, 1705062). Images were acquired with ChemiDoc camera (Biorad ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibodies: goat anti-rabbit HRP (BioRad 1706515 or Invitrogen 65-6120, 1:5,000), anti-mouse HRP (Santa Cruz sc-525409 ...
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bioRxiv - Microbiology 2022Quote: ... either HRP-conjugated goat anti-rabbit or an anti-mouse antibody (1:5,000, BioRad). Antibodies were detected using the enhanced chemiluminescence system (Immobilon Forte Western HRP substrate ...
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bioRxiv - Systems Biology 2023Quote: ... The secondary antibody used was 1:5000 HRP goat anti-rabbit (Bio-Rad #1706515).
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: rat anti-CD11b (Bio-Rad, #MCA711G, 1:500), and rabbit anti-Iba1 (Wako ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with an anti-F4/80 antibody (1:100 dilution, Bio-Rad, cat ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: rat anti-CD11B (1:100; #MCA711G Bio-Rad), rabbit anti-IBA-1 (1:100 ...