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Citations for Bio-Rad :
301 - 350 of 3840 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated for 1 h at RT with goat anti-Lipid A LPS antibodies (diluted 1:1,000 in blocking buffer; Bio-Rad; Cat # OBT1844). Blots were washed three times with TBST (50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931; goat anti-rabbit IgG-HRP, 1:5000, Bio-Rad, 1706515 ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Neuroscience 2023Quote: ... The blot was washed 3 times with TBST for 5 minutes and was incubated with 10 mL of 3% BSA/TBST (w/v) with 1 µL anti-mouse-HRP secondary antibody (1:10,000; Bio-Rad, 170-6516) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Antibody binding was detected with goat antihuman IgG conjugated to phycoerythrin and quantified on a Bio-Plex Suspension Array System (Bio-Rad, Hercules, CA, USA). Weighted median fluorescence intensity (MFI ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 h incubation with the respective secondary antibodies: StarBright700 anti-rabbit (10000068187, 1:5000) or StarBright700 anti-mouse (10000068185, 1:2500) both from BioRad (Hercules, CA, USA). Four washes with TBS-T were performed after incubation with secondary antibodies ...
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bioRxiv - Genetics 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated antirabbit (1: 2500, 170-6515) or antimouse (1: 5000, 170-6516) IgG secondary antibodies (BioRad, Hercules, CA, USA). Proteins were detected using the SuperSignal chemiluminescent substrate kit (Pierce).
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bioRxiv - Molecular Biology 2020Quote: ... We used horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Bio-Rad, 1:2000). Blots were developed using an enhanced chemiluminescence kit (Amersham ...
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bioRxiv - Bioengineering 2022Quote: ... or rat anti-F4/80 (rat anti-F4/80 antibody, 1:50, BioRad, MCA497GA, Hercules, CA). Cells were again washed in DPBS and incubated for 1 h at room temperature with Alexa Fluor® 488-conjugated secondary antibody (goat polyclonal antibody to rabbit IgG ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% Tween 20 (TBST) followed by 1hr incubation with secondary antibody in TBST (1:5000, Biorad). Three washes were given for 10 minutes each after the secondary antibody incubation ...
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bioRxiv - Immunology 2019Quote: ... and a primary rat anti-mouse CD68 antibody (activated microglia/macrophages, 1:200 dilution, MCA1957, Biorad) with secondary goat anti-rat Alexa-Fluor 555 (1:200 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... three sections of tissue were stained with antibodies against proteolipid protein (MCA839G; Bio-Rad; 1:1000) and imaged at a spatial resolution of 0.28 µm/pixel ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: goat anti-rabbit HRP (1:5000, Bio-Rad: 170-5046) and goat anti-mouse HRP (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000 ...
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bioRxiv - Biochemistry 2021Quote: ... they were detected with goat anti-rabbit-HRP antibody (Bio-Rad catalog #170-6515; 1:10,000) diluted in TBST with 2% milk ...
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bioRxiv - Microbiology 2022Quote: ... a 1:20,000 dilution of HRP-conjugated goat anti-rabbit secondary antibody (Bio-Rad, Hercules, CA), was incubated with the membranes at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... The following primary antibodies and concentrations were used: mouse anti-CD44 (1:500, Bio-Rad MCA5762), mouse anti-Laminin (1:500 ...
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bioRxiv - Systems Biology 2020Quote: ... for the primary and 1:10,000 goat anti-rabbit HRP-labeled antibody (170-5046, Bio-Rad) for the secondary.
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bioRxiv - Biochemistry 2021Quote: ... surface CD8 was labeled with 10 µg ml−1 AlexaFluor647-anti-CD8 antibody (Bio-Rad, MCA1226A647) at 37 °C for 5 min ...
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bioRxiv - Genetics 2021Quote: ... tissue sections were stained with F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... secondary horseradish-peroxidase-conjugated goat anti-rabbit antibody (1:2,000; Bio-Rad, Cat# 166-2408EDU, RRID:AB_11125345), secondary horseradish-peroxidase-conjugated goat anti-mouse antibody (1:2,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Western blotting was performed using the following antibodies: anti-vinculin (MCA465GA, Bio-Rad; 1:1,000 dilution), anti-FOSB (2251 ...
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bioRxiv - Molecular Biology 2023Quote: ... Second antibodies were added for another 1 h and detected with Clarity Western ECL Substrate (Biorad). Immunoreactive bands were exposed in an automatic X-ray film processor ...
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bioRxiv - Microbiology 2022Quote: ... Commercial antibodies were used for β-tubulin (clone YL1/2, Bio-Rad, Watford, UK; 1:5000), β-actin (Abcam ab8227 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This was followed by incubation with secondary HRP-conjugated anti-mouse antibody (1:2000; Bio-Rad). Proteins were visualized using the Pierce ECL system (Genetica/Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies were used: goat α-mouse (1:10 000, Bio-Rad, 170-6515), donkey α-goat (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were probed with either secondary antibodies goat anti-mouse-HRP (Bio-Rad, 1706516, 1:5000) or goat anti-rabbit-HRP (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... P-ATG16L1-S278 antibody was diluted at 1:4000 in EveryBlot blocking buffer (Bio-Rad, 12010020) and incubated overnight ...
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bioRxiv - Neuroscience 2024Quote: ... blots were incubated for 1 hour at RT with appropriate HRP-conjugated secondary antibodies (Bio-Rad) diluted 1 in 3,000 in TBST with 5% milk ...
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bioRxiv - Biophysics 2024Quote: ... The coverslips were incubated with the primary antibody [anti-tyrosinated tubulin 1:150 (MCA77G, Bio-Rad)] in blocking solution (10% FBS with 0.05% Tween 20 diluted in cytoskeleton buffer overnight at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... and secondary antibody goat anti-rabbit IgG (H+L)-HRP-conjugate (1:10,000 dilution, Bio-Rad). Image lab (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used in this study: sheep polyclonal anti-TGN46 (#AHP500G, BioRad, 1:2000), rabbit polyclonal LRBA antibody (#HPA023567 ...
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bioRxiv - Plant Biology 2021Quote: ... Protein concentrations were quantified using the Coomassie Blue dye binding method (Bradford, 1976) using a concentrated dye purchased from BioRad (500-0006, Hercules, CA, USA). AsA and DHA concentrations were determined by an ascorbate oxidase based method according to Lin et al ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... the beads were then washed five times with fresh binding buffer before being extracted with 30 µL of 2X Laemmli Buffer (Bio-Rad Laboratories, Hercules, CA, USA). 10 μL of the samples were separated via SDS-PAGE and stained with SimplyBlue safe stain while 1 μL of the samples were separated via SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then washed five times with fresh binding buffer before being extracted with 30 µL of 2X Laemmli Buffer (Bio-Rad Laboratories, Hercules, CA, USA). For Figure 5 E ...
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bioRxiv - Microbiology 2020Quote: ... Detection via secondary antibody was done with goat α-rabbit mAb (1:5000) conjugated to HRP (Biorad). Immunoblots were developed using the SuperSignal West Femto (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500, Bio-Rad, Cat# 12005870, RRID: AB_2884949) and hFAB Rhodamine Anti-Tubulin Antibody (1:2500 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used for western blot and IF include: goat anti-rabbit HRP (1:10,000, BioRad), goat anti-mouse HRP (1:10,000 ...
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bioRxiv - Genetics 2021Quote: ... or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706516). Membranes were incubated in ClarityTM western ECL substrate (Bio-Rad laboratories ...
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bioRxiv - Genetics 2019Quote: ... incubated in goat anti-rabbit IgG horseradish peroxidase secondary antibody (1:3000; Bio-Rad, Hercules, CA, USA) for 1 hour at room temperature ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a one hour incubation in the appropriate secondary antibodies coupled to peroxidase (1:1000, Biorad). Proteins were detected by enhanced chemiluminescence detection (ECL ...
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bioRxiv - Plant Biology 2022Quote: ... and then with horseradish peroxidase–conjugated Goat Anti-Rabbit IgG antibodies (1:15000 dilution, Bio-Rad, 1706515) or Goat Anti-Mouse antibody (1:15000 dilution ...
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bioRxiv - Biochemistry 2019Quote: ... and for AAC1 the primary antibody was 1:5000 dilution of rabbit IgG anti-AAC1 (Bio-Rad). The secondary antibody was 1:5000 dilution of horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2021Quote: Mouse monoclonal anti-Viral V5-TAG antibody (1:5000; clone SV5-Pk1) was purchased from Bio-Rad/AbD Serotec ...
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bioRxiv - Pathology 2023Quote: ... at a dilution of 1:100 and fibrinogen using the monoclonal anti-fibrinogen antibody (#4440-8004, BioRad) at a dilution of 1:200 incubating over night at 4° C in a humidified chamber ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted 1:1000 in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...