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Citations for Bio-Rad :
251 - 300 of 3840 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with an anti-F4/80 antibody (1:100 dilution, Bio-Rad, cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti-tubulin Rhodamine antibody (AbD22584, 1:5000, Bio-Rad Laboratories, Hercules, CA, USA) were used for immunofluorescence detection ...
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bioRxiv - Immunology 2023Quote: ... followed by overnight incubation with F4/80 antibody (Clone CI:A3-1, Bio-Rad Laboratories) diluted in DAKO antibody diluent (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibody used was goat anti-rabbit-HRP (1:5000, Bio-Rad, 170-6515). Ponceau-S red staining was used for mESCs samples as a loading control.
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated with 1:5000 HPRT-conjugated anti-mouse secondary antibody (Biorad, 1706516) for 1 h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated in secondary goat anti rabbit antibody (1:10000, Bio-Rad, 1706515) for one hour ...
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bioRxiv - Cell Biology 2024Quote: ... followed by horseradish peroxidase-conjugated Goat- anti-mouse second antibodies (1:10000; BioRad # 1721011) for 1hr at the room temperature.
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bioRxiv - Developmental Biology 2023Quote: ... and secondary antibodies HRP-conjugated goat anti-mouse IgG (1:5000; 1706516, Bio-Rad) or goat anti-rabbit IgG (1:5000 ...
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bioRxiv - Genetics 2024Quote: ... The primary antibodies (NF-kB, #8242 Cell Signaling, 1:1000; CD3, MCA-1477 Biorad, 1:100 ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 10μl of EMSA binding reactions were loaded on 5% nondenaturing polyacrylamide Mini-PROTEAN TBE gels (Catalog #4565015, Bio-Rad Laboratories, Hercules, CA) for electrophoretic analyses ...
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bioRxiv - Cancer Biology 2020Quote: ... To reduce non-specific binding single cell suspensions were incubated for 5 min at 4°C with PBS/10% AB-serum (Bio-Rad, Germany), subsequently stained with fluorescence-labeled or biotinylated antibodies for 15 min at 4°C and washed once with PBS/2% FCS/0.01% NaN3 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and stored at -80 until α5 subunit and its binding partners were immunoprecipitated using SureBeads protein G magnetic beads according to the manufacturer’s protocol (Biorad, Hercules, CA, USA). Western blots (detailed in section 2.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent, Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Microbiology 2022Quote: ... a 1:1000 dilution of α-FLAG M2 monoclonal antibodies and/or 1:10,000 dilution of HRP-labeled α-rabbit secondary antibodies (BioRAD Catalog #170-6515) on nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... for the rapamycin blot or with the fluorescent StarBright Blue 700 anti-mouse (1:5000) and StarBright Blue 700 anti-rabbit (1:5000) secondary antibodies (Bio-Rad) for the MRT68921 blots in TBS-T containing 5% powder milk ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred onto PVDF membrane and probed with primary antibody (Table 1) at a dilution of 1:1000 prepared in 5% blotting grade blocker (Bio-Rad) for 12-16 h at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 1:5000), or donkey anti-goat antibody (sc-2020, 1:5000) and signal was detected with enhanced chemiluminescence substrate (Bio-Rad).
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bioRxiv - Bioengineering 2023Quote: ... probed with a goat anti-mouse or goat anti-rabbit IgG H+ L-HRP conjugated secondary antibody (1:5000-1:10000) (Bio-Rad) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated at RT for 1 h with HRP-linked secondary antibodies (Bio-Rad 170-6515 and 172-1011, 1:5000), imaged via chemiluminescence on a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... Native protein extracts from E.coli or Synechococcus ΔCS were incubated in IMAC HEPES binding buffer supplemented with potential effector molecules and Ni-charged iminodiacetic acid Profinity™ IMAC Resin (Bio-Rad, USA) on a low speed rotator for 1 h at 23°C ...
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bioRxiv - Microbiology 2023Quote: ... The remaining flow through was incubated overnight with 1 mL Strep-Tactin® Sepharose® beads (iba-lifesciences) equilibrated with Binding Buffer and applied to a Poly-Prep chromatography column (Bio-Rad). Flow through was run twice on the column ...
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bioRxiv - Developmental Biology 2020Quote: ... secondary antibody diluted in TBST containing 0.5% BSA with streptavidin-HRP (Bio-Rad, 1:10,000) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected using horseradish peroxidase-conjugated goat anti-rabbit IgG (1:10,000, BioRad) and Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... We used horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Biorad, 1:2,000). Blots were developed using an enhanced chemiluminescence kit (Amersham ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were developed with a 1:5000 dilution of the HRP-conjugated secondary antibody (BioRad). Proteins were visualized ...
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bioRxiv - Cell Biology 2021Quote: ... StarBright® B700 labeled goat anti-mouse IgG secondary antibody (Bio-Rad, 12004158, 1:5000), HRP conjugated goat anti-mouse IgG (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... and secondary antibody Goat Anti-Mouse (H+L) HRP Conjugate (1:250; BioRad #170-6516). The samples were then incubated with peroxidase (HRP ...
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bioRxiv - Plant Biology 2022Quote: ... followed by a goat anti-rabbit StarBright Blue 700 antibody (Bio-Rad, 1:2500 dilution). The signal was detected using the multi-plex function of the ChemiDoc MP imager (Bio-Rad)
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bioRxiv - Bioengineering 2021Quote: ... followed by 1:4000 dilutions of HRP-conjugated immunoglobulin G antibodies (Bio-Rad, Hercules, CA). Detection was performed using an enhanced chemiluminescent substrate (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... Immune cell infiltrates were stained using the following antibodies: anti-CD3 (1:150; Bio-Rad) and anti-F4/80 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were detected using Goat anti-Rabbit IgG-HRP conjugate (1:10000, #1706515, Bio-Rad) or Goat anti-Mouse IgG-HRP conjugate (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... TBS-T) before incubation with HRP-conjugated secondary antibody for 2 hours (Biorad, 1:1000). After 3 times washes with 5 min intervals with TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Bio-Rad, 1:10000) were visualized using ECL Plus Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins were probed with secondary antibodies goat anti-mouse-HRP (Bio-Rad, 1706516, 1:5000) or goat anti-rabbit-HRP (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... secondary horseradish-peroxidase-conjugated goat anti-mouse antibody (1:2,000; Bio-Rad, Cat# STAR105P, RRID:AB_323002).
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bioRxiv - Neuroscience 2022Quote: ... The tertiary antibody solution contained DyLight 550 mouse anti-V5 (1:500, Bio-Rad, #MCA1360D550GA).
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bioRxiv - Cell Biology 2023Quote: ... and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP) (BioRad, 1:5000). The detection of HRP was accomplished using the PierceTM ECL Western Blotting Substrate (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP) (BioRad, 1:4000) were used ...
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bioRxiv - Microbiology 2023Quote: ... goat anti-rabbit IgG HRP-conjugated secondary antibody (1: 3,000 dilution, BIO-RAD 170-6515). Proteins were detected with Chemi-Lumi One (Nacalai Tesque ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1 hour at room temperature with HRP-conjugated secondary antibodies (BioRad). Three washing steps of 5 minutes in PBS–Tween20 (0.1–0.3% ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were utilized: Rat Anti-Myelin Basic Protein (MBP; 1:500, Biorad) and Rabbit Anti-Oligodendrocyte transcription factor 2 (Olig2 ...
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bioRxiv - Physiology 2023Quote: ... followed by overnight staining with pig specific CD45 antibody (clone K252.1E4, Bio-Rad, 1:200). After secondary ab and DAPI applications ...
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bioRxiv - Microbiology 2023Quote: ... followed by secondary rabbit antibodies at a concentration of 1:3000 (Biorad, Cat. No. 1705046). Both primary and secondary antibody were suspended in 1x TBS-T with 5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies for Westering blotting were goat anti-mouse IgG HRP (Bio-Rad; 1:3000) and polyclonal goat anti-rabbit IgG HRP (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated in anti-rabbit HPR conjugate antibody (1:5000, 170-6515, Bio-Rad) in PBS for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and incubated overnight at 4°C in primary antibodies: anti-human actin-β primary monoclonal antibody produced in mouse (1:100; Bio-Rad Laboratories Inc. MCA57766A) and non-muscle myosin II produced in rabbit (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in 10% normal donkey serum, and then stained in antibodies overnight (rabbit anti-Iba1, 1:500, Wako and CD68 (Biorad 1:100)) ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained immunofluorescence labelling of microglia using an Iba-1 antibody (Wako, Richmond, USA) with a dilution of 1:200 co-stained with CD68 (BioRad, California, USA) with a dilution of 1:100 ...