Labshake search
Citations for Bio-Rad :
4901 - 4950 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature before signal development with Western ECL Substrate (Bio-Rad Laboratories, #1705061) and imaging under ChemiDoc XRS+ System (Bio-Rad Laboratories).
-
bioRxiv - Biochemistry 2022Quote: ... 2 μl of diluted vRNP mixtures was loaded onto a 5% polyacrylamide native TBE gel (Bio-Rad) and run at 125 V for 80 min at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... boiled for 2 min and then analyzed on a 4–20 % SDS-PAGE gradient gels (Bio-Rad). The protein bands were then transferred to a nitrocellulose membrane ...
-
bioRxiv - Immunology 2020Quote: ... and detergent removed by adsorption to polystyrene BioBeads SM-2 (3 mg; Bio-Rad; overnight, 4°C). Proteoliposomes were collected by ultracentrifugation (180,000 xg ...
-
bioRxiv - Immunology 2019Quote: ... fluorescence images were acquired before and after a 2 min incubation after trypan blue (0.1%; Bio-Rad), which was added carefully to minimize sample movement ...
-
bioRxiv - Cell Biology 2019Quote: ... RT-qPCR was performed on 2 μl of 1/25 diluted cDNA with a CFX-96 (Biorad) thermocycler ...
-
bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad) using the following oligonucleotides ...
-
bioRxiv - Molecular Biology 2019Quote: ... 0.009% H2O2) for 2 min and images were taken with a ChemiDoc Imaging System fitted (Bio-Rad). Densitometry was performed with Image Lab 5.0 software (Bio-Rad) ...
-
bioRxiv - Genomics 2021Quote: ... RT-PCR amplicons were electrophoresed on 2 % agarose gels and imaged on a ChemiDoc MP (Bio-Rad). The resulting images were analyzed using ImageJ (v1.52a) ...
-
bioRxiv - Microbiology 2020Quote: ... or 500 ng to 3 µg for integrative plasmids (MicroPulserTM electroporator Biorad in 2 mm cuvettes (Eurogentec) at 25 µF ...
-
bioRxiv - Immunology 2021Quote: ... 2-ul of cDNA was added to qPCR reaction containing iQ SYBR green master mix (Bio-Rad) and 10-pmol of gene-specific primers ...
-
bioRxiv - Microbiology 2022Quote: ... 400 Ω) was performed in a 2-mm cuvette using the Gene Pulser Xcell Electroporation System (BioRad). After electroporation ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 2 minutes at 72°C using the C1000 Touch Thermal Cycler (Bio-Rad, Los Angeles, CA). Primer sequences are listed in the Supplemental Methods.
-
bioRxiv - Cell Biology 2022Quote: ... The detergent was then removed by four successive additions of Bio-Beads SM-2 Resin (Bio-Rad) over the course of ∼24 h at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Supernatant was mixed with sample loading buffer supplemented with 1% v/v 2-mercaptoethanol (BioRad, Cat #1610710). Sample was not heated to prevent aggregation of the GPCR ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 μL of the diluted samples were used as input in a ddPCR supermix (Bio-Rad, 1863023). Primers and probes ...
-
bioRxiv - Biochemistry 2024Quote: ... A hydroxyapatite column was prepared by resuspending 2 g Bio-Gel HTP Hydroxyapatite (Bio-Rad, 130-0420) in 12 ml CDC6 HTP-wash buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4-15% Tris-Glycine gel (Bio-Rad Cat#5678084) using Tris-Glycine running buffer (0.2M Tris-HCl ...
-
bioRxiv - Microbiology 2024Quote: ... 1.5 or 2 % agarose gel solutions (50 mL total volumes) were prepared with agarose powder (Bio-Rad) using 0.5× TBE buffer (45 mM Tris ...
-
bioRxiv - Neuroscience 2024Quote: ... or at 100 V for 2 h at 4 °C using the wet transfer method (Bio-Rad Mini Trans-Blot Electrophoretic Cell 170-3930) ...
-
bioRxiv - Biochemistry 2022Quote: ... with integration time set to 2 s and imaged using a Gel Doc XR system (Bio-Rad).
-
bioRxiv - Cell Biology 2023Quote: ... Lysis was performed by directly adding 2x Laemmli sample buffer (supplemented by 2-mercaptoethanol, Bio-Rad Laboratories) at a ratio of 1 µL per 0.1 mg of cell pellets and pipetting up and down ...
-
bioRxiv - Microbiology 2023Quote: ... The relative gene expression was analysed by the comparative 2-ΔΔCt method using CFX 96 software (BioRad). Data were normalized with the endogenous control ...
-
bioRxiv - Biophysics 2022Quote: ... DDM was removed by addition of a 100-fold excess (w/w) of biobeads SM-2 (Biorad) compared to protein for 4 h at 4 °C under gentle agitation ...
-
bioRxiv - Systems Biology 2023Quote: ... Time points were measured every 2-3 days by flow cytometry analysis of >10,000 cells (BioRad ZE5). For experiment with BRM014 (MedChemExpress #HY-119374) ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were lysed in sample buffer (2× Laemmli sample buffer, No. 1610737, Bio-Rad, Hercules, California, USA). Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Neuroscience 2023Quote: ... or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution, clone R73, Bio-Rad #MCA453GA) from day 9 to day 14 to deplete DRG T cells as previously described 66 ...
-
bioRxiv - Physiology 2023Quote: ... Captured complexes were dissociated from the beads in the ×2 Laemmli buffer (Bio-Rad Laboratories, Hercules, CA) and analyzed by SDS-PAGE/western blotting using chemiluminescent substrate (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining was done in 2% milk-TBST (+ 0.01% sodium azide) and detected using ChemiDoc (Biorad).
-
bioRxiv - Biochemistry 2024Quote: ... Detergent removal was carried out at 4°C via incubation with Bio-Beads SM-2 (Bio-Rad) at a concentration of 200 mg ml-1 for three times with two hours for each time ...
-
bioRxiv - Plant Biology 2024Quote: ... centrifuged for 2 minutes before running through 4-20% mini Protean TGX Stain Free Gel (BioRad, USA). The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore ...
-
bioRxiv - Systems Biology 2024Quote: ... containing 2 μl of cDNA template was prepared and analyzed using CFX Connect Real-Time instrument (BioRad) with the following thermocycling conditions ...
-
bioRxiv - Immunology 2024Quote: ... cells were stimulated with 5 μg/ml anti-Igκ plus anti-Igλ F(ab’)2 antibodies (BioRad). After 5 min of data acquisition ...
-
bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended to OD 10 in 1X Laemmeli Buffer supplemented with 2-mercaptoethanol (10%) (Bio-Rad) and boiled at 99°C for 10 minutes ...
-
bioRxiv - Physiology 2019Quote: ... Assays in 50 μL volume were performed in 96-well microplates with absorbance measurements at 560 nm on an iMark™ Microplate Reader (Bio-Rad, Hercules, CA, USA). Total catalase activity was determined using the Amplex® Red Catalase Assay Kit (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... samples were rinsed again three times with wash buffer and resuspended in 100 μL per well prior to the read-out on a Bio-Plex® 200 multiplex array reader (Bio-Rad Laboratories, Hercules, USA). Using the Bio-Plex Manager™ software a standard curve was created for each analyte by performing a five-parameter logistic curve-fit ...
-
bioRxiv - Physiology 2020Quote: ... and an equal amount of total protein (30 μg) per sample was subjected to standard SDS-PAGE using Mini-PROTEAN® TGX™ 4-20% 12-wells gels (Bio-Rad, Hercules, CA). Gels were electroblotted onto PVDF membranes (Bio-Rad ...
-
Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... from ART or ART + GYY4137 treated groups were harvested to isolate total RNA using Qiagen RNAeasy isolation kit and 200 ng of total RNA was reverse transcribed (iScript™cDNA synthesis kit, Bio-Rad). Reverse transcribed cDNA was diluted 10-fold and amplified using primers against HIV LTRs and seminested – PCR was performed using primers and probe listed in Table S6 ...
-
bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
-
bioRxiv - Physiology 2020Quote: ... RNA of treated cells was isolated using EZ-RNA isolation kit (Biological Industries Beit Haemek, Israel) and cDNA was synthesised using iScript cDNA synthesis kit (Bio-Rad, UK). Real-time qPCR analyses were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Genetics 2021Quote: ... we purified total RNA using the Qiagen RNeasy Mini kit (Cat#74106) and made cDNA using iscript cDNA synthesis kit from Biorad (Cat#1725036), followed by a Taqman assay to detect AHR transcription (Hs00169233-m1 for AHR expression and Hs0442063-g1 for GAPDH ...
-
bioRxiv - Biochemistry 2021Quote: RT-qPCR was performed using the 2019-nCoV RUO Kit (Integrated DNA Technologies, #10006713) in combination with the iTaq™ Universal SYBR® Green One-Step Kit (BioRad). Each 20 μl reaction contained 10 μl 2X iTaq universal SYBR® Green reaction mix ...
-
bioRxiv - Evolutionary Biology 2021Quote: RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Cat# 74104) and cDNA was prepared using the iScript cDNA Synthesis Kit (Biorad, Cat# 1708890). Assays were done in triplicate using Express SYBR GreenER master mix (Invitrogen ...
-
Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... Whole-larvae cDNA was generated by isolating total RNA from 3–5 dpf zebrafish larvae using the E.Z.N.A Total RNA kit (Omega, Bio-Tek INC, Cat# R6834-01) and reverse transcribing using the iScript cDNA synthesis kit (BioRad, Cat# 1708891). Fragments of coding sequences of these genes were amplified by PCR using gene-specific primers as follows:
-
bioRxiv - Bioengineering 2024Quote: ... RNA was extracted using a total RNA purification kit (NORGEN, Auburn, WA) followed by cDNA synthesis utilizing iScript cDNA kit (BIORAD, Hercules, CA). Quantitative real time-PCR using iTaq Universal SYBR GREEN Supermix (BIORAD ...
-
bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
-
bioRxiv - Plant Biology 2021Quote: ... were placed on 0.8% water-agar plates and transformed using biolistic bombardment with the PDS-1000/He™ system (Bio-Rad, Hercules, California, USA) as described above ...
-
bioRxiv - Plant Biology 2021Quote: ... three primary leaves of 7 d old wildtype barley plants (cultivar Golden Promise) were placed on 0.8% water-agar plates and transformed using the PDS-1000/He™ system (Bio-Rad, Hercules, California, USA). For one transformation reaction ...
-
bioRxiv - Bioengineering 2023Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 seconds at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 10°C during the LC-MS measurements ...
-
bioRxiv - Microbiology 2023Quote: ... United States) with adjusted concentrations of divalent cations Ca2+ and Mg2+ or on Mueller-Hinton Agar (MHA) plates (Bio-Rad, Marnes-la-Coquette, France). In conjugation experiments ...