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Citations for Bio-Rad :
5101 - 5150 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The content of each well was then drawn up into the Bio-Plex 100 System array reader (Bio-Rad Laboratories Bio-Rad Laboratories, Hercules California, USA), which identifies and quantifies each specific reaction based on bead color and fluorescent signal intensity ...
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bioRxiv - Developmental Biology 2020Quote: ... Upon removal of genomic DNA (TURBO DNA-free™ Kit or RNase-free DNase Set) cDNA was synthesized using the iScript™ cDNA Synthesis Kit (Bio-Rad). PCR reactions were run in triplicate with 6.25-12.5 ng cDNA per reaction using the CFX96 Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Physiology 2023Quote: ... protein content in the soluble fraction of homogenates was measured using a RC-PC protein assay kit (DC Protein Assay Kit, Bio-Rad, Hercules, CA), and then dissolved in Laemmli buffer.
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Immunology 2022Quote: ... Complementary DNA was synthesized using the reverse transcription kit (PrimeScript RT reagent Kit) and quantitative (q)PCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The housekeeping gene GAPDH was used for reference ...
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Cell Biology 2020Quote: ... were harvested and resuspended in 1X PBS-NaN3 (0.2%) and embedded in 2% low-melt agarose (Mb grade, BioRad). Agarose plugs were chilled at 4°C for 30 min and then ejected into Proteinase K digestion buffer (1 mg/mL proteinase K ...
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bioRxiv - Biophysics 2022Quote: ... Nanodisc reconstitution was induced by removing detergent with 0.8 mg ml-1 pre-washed Biobeads SM-2 (Bio-Rad) for 2 hours with gentle agitation at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated in 2-mm cuvettes (600 V, 50 μF, 200 Ω) by using Gene Pulser Xcell (Biorad). Immediately after electroporation ...
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bioRxiv - Molecular Biology 2021Quote: ... resolved by size on a 2% agarose gel and imaged on Gel Doc XR+ and ChemiDoc XRS+ systems (Biorad).
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bioRxiv - Cell Biology 2022Quote: ... The reaction mixture (total volume 22 μl) contained 11 μl of 2× ddPCR Super Mix for Probes (BioRad, 1863024), 1.1 μl of 4 primers mixture 18 μM each ...
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bioRxiv - Pathology 2019Quote: ... by SDS-PAGE at 100 V for 2 hours in a Mini-PROTEAN® Tetra cell apparatus (Bio-Rad). The Kaleidoscope commercial standard Precision Plus Protein Standard (Bio-Rad ...
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bioRxiv - Pathology 2019Quote: ... The cDNA was then diluted 10x with water and 2 µl was mixed with iQ SYBR Green Supermix (BioRad) containing 100 nM of forward and reverse primers ...
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bioRxiv - Genetics 2020Quote: ... 2 mM ATP and 1 mM DTT and the reactions were stopped by addition of Laemli sample buffer (BioRad). Samples were run on precast 4-20% gradient SDS-PAGE gels (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed in 20 μL reactions in triplicate containing 10 μL 2 × SsoFast EvaGreen Supermix (Bio-Rad,), 1 μL of primers (3 μM F and 3 μM R) ...
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bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 150 mM KCl and 2 mM MgCl2) at 37°C for 16 hours in thermal cycler (Bio-Rad, USA). The samples were separated using 10% non-denaturing polyacrylamide gel and tris/boric acid/EDTA buffer (TBE ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were run on 2% agarose gel and visualized in a Gel Doc EZ System (Bio-Rad, USA). GAPDH served as an endogenous control.
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and 30μg of total protein per sample was mixed with Laemmli buffer supplemented with 2-mercaptoethanol (10%) (Bio-rad) and boiled at 99°C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... culture supernatants were collected and cells were directly lysed with 150 μl of 2× laemmli buffer (Bio-rad, 1610737). Proteins in culture supernatants and cell lysates were separated by SDS-PAGE and transferred onto PVDF membranes by using the Trans-Blot Turbo system (Bio-rad) ...
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bioRxiv - Cell Biology 2020Quote: ... differentiated organoids and differentiated monolayers) and was calculated using the 2-ΔΔCt method using CFX Maestro software (Bio-Rad). Transcription was normalised to the expression levels of the housekeeping gene (18S ribosomal RNA ...
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bioRxiv - Plant Biology 2020Quote: ... Each qRT-PCR reaction of CZ and ZC was performed by the manufacturer’s instructions of EvaGreen Express 2×qPCR MasterMix (abm, Cat. No.MasterMix-ES, http://www.abmGood.com/) and BioRad®CFX96 Real-Time PCR system (Bio-Rad). Relative expression was quantified with the geometric mean of internal reference genes ETIFI (eukaryotic translation initiationfactor 1) ...
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bioRxiv - Cell Biology 2020Quote: ... Total numbers of viable cells were determined after 2 and 4 days using the TC10 cell counter (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR reactions were conducted using 10 µL 2× SYBR-Green Universal Master Mix (Bio-Rad, Hercules, CA, USA), 2 µL of forward primer (9 mM) ...
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bioRxiv - Immunology 2021Quote: ... Bound PRRSV-2-specific IgG was detected using goat anti-pig IgG conjugated with horseradish peroxidase (HRP; Bio-Rad) diluted at 1:10,000 in blocking buffer for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: Frozen PBMCs were thawed, washed in FACS buffer (2% FBS, 1mM EDTA in PBS) and counted on TC20 (Biorad) before surface staining using the following panel ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of cDNA was mixed with 2 μL of forward and reverse primers (2.5 μM each, Table S1,) and 1x SYBR Green Supermix (BioRad) for a final reaction volume of 20 μL ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein levels were quantified using BCA Assay (Pierce) and supplemented with 4x Laemmli Buffer with 2-mercaptoethanol (Biorad). Samples were boiled and sonicated ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells:DNA mixture was transferred to an ice-cold 2 mm gap electroporation cuvette (Molecular Bioproducts) and electroporated at 2.5 kV with a MicroPulser (BIORAD). One ml of Super Optimal broth with Catabolite repression (SOC ...
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bioRxiv - Plant Biology 2022Quote: ... Each RT-qPCR reaction (10 μl) included 2 × iTaq™ Universal SYBR®Green Supermix (Bio-Rad, CA, USA), 500 nM forward and reverse primers (Supplementary File 1C) ...
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bioRxiv - Molecular Biology 2022Quote: ... The SARS-COV-2 NTD expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences) ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 2% w/v CA and treated with (7% w/v) Chelex100 resin (Bio-Rad, Mississauga, ON, Canada) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitants were then gently washed three times with KPBS and eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis.
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of cDNA was added to 10 µL of Bio-Rad ddPCR Supermix for Probes (1863024; Bio-Rad), followed by the addition of 1 µL of TaqMan miR-146a-5p 20X primer mix (4427975 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT reactions were diluted 2-fold and qPCR was performed in duplicate with SsoAdvanced SYBR Green Supermix (Bio-Rad) according to manufacturer’s protocols with qPCR_FWD and qPCR_REV primers from IDT (see below) ...
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bioRxiv - Developmental Biology 2024Quote: ... centrifuged at 14,000rpm at RT for 2 minutes and separated on precast 10% SDS-PAGE gels (4561036, Bio-Rad) at 100V for 70 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 2 % fetal bovine serum and 8 % chelated fetal bovine serum using Chelex® 100 Resin (BioRad, Ref. 142-1253). To differentiate HaCaT cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1×108 concentrated fungal protoplasts (37 μL) (64) were mixed with 2% CleanCut Agarose (63 μL, Bio-Rad, #1703594). The mixture was then loaded into a plug mold (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: Diluted mouse serum (1:100 in 0.1% BSA in PBS) was incubated on HEp-2 slides (MBL or BioRad) for 1 hour in the dark at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... The heart tissue samples were then combined with 2 x Laemelli buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Protein samples were then separated on 7.5 to 20% v/v sodium dodecyl sulfate (SDS ...
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bioRxiv - Microbiology 2023Quote: ... using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Plasmid sequences were confirmed by sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... Each 100 μl aliquot was transformed with 0.1 pmol of EZ-Tn5
Tnp Transposome using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Each reaction was allowed to recover in 500 μl SOC (2% tryptone ... -
bioRxiv - Microbiology 2023Quote: ... 2 min at 72°C for final extension in a T100 Thermal Cycler (BIO-RAD Lab. Inc., Hercules, CA). The sizes of PCR products were checked by running agarose gel electrophoresis ...
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bioRxiv - Biophysics 2023Quote: ... slides were prepared with unlabeled microtubules attached to the slide surface via an anti-tubulin antibody (BioRad YL1/2). Kinesin-liposome complexes were added to the flow cell and imaged in TIRF ...
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bioRxiv - Biophysics 2023Quote: ... Bio-Beads SM-2 (#152-3920) and the enhanced chemiluminescence (ECL) western blotting reagent (#170-50001) from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... MEC samples were directly homogenized using 2x Laemmli Sample Buffer and supplemented with 2-mercaptoethanol (β-mercaptoethanol) (BioRad Hercules). Proteins were separated by electrophoresis on a polyacrylamide gradient gel (BioRad Hercules ...
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bioRxiv - Biochemistry 2024Quote: A 22ul reaction mixture was prepared comprising 11ul 2×ddPCR Supermix for probes (no dUTP) (Bio-Rad, CA, USA), 0.55 ul of TRECs primers (36uM stock ...