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Citations for Bio-Rad :
4851 - 4900 of 9194 citations for Glutathione Fluorescent 384 Well Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: iScript Advanced cDNA Synthesis Kit for PCR (BioRad) was used for Reverse Transcription (RT ...
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bioRxiv - Genomics 2024Quote: ... iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) was used to reverse transcribe one βg total RNA according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The iScript Reverse Transcription Supermix kit (Bio-Rad) was used to synthesize cDNAs from 200 ng of RNA according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Experiments were run using 26-well gels (Criterion TGX Precast Gels, 15 μl, 1.0 mm, 4-20%, Bio-Rad Laboratories, Inc. Cat No 5671095). 13 wells were loaded by one researcher and the other set of 13 wells were loaded by another researcher for each set of experiments ...
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bioRxiv - Physiology 2020Quote: ... and equal amounts of total protein (15 or 20 μg) were loaded in different wells on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad, Australia). A stain-free system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a cellulose acetate membrane with pores with a 0.2 μm diameter (Whatman ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Twenty microlitres of the mastermix + template DNA were pipetted into the very bottom of the central row of (8) wells of the Droplet Generator DG8™ Cartridge (Bio-Rad 186-4008). Sixty-five microlitres of droplet generation oil for probes (BioRad 186-3005 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100 μl of dimethylsulfoxide was added to each well and the absorbance was measured at 570 nm using a microplate reader (Bio-Rad, Hercules, CA, USA). According to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a 0.2 μM cellulose acetate membrane (Whatman ...
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The effect of exercise on the protein profile of rat knee joint intra- and extra-articular ligamentsbioRxiv - Molecular Biology 2020Quote: ... 10 μg of ACL control and exercise samples were electrophoresed and separated on a pre-cast 12 well gel (Bio-Rad Criterion 10% TGX). Separated proteins were then transferred to a nitrocellulose membrane and blocked with 10 ml LICOR Odyssey® blocking buffer (LI-COR ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and combined with 50 μM of forward and reverse primers (for primer sequences, please see Supplementary Table 1) as well as iQ™ SYBR® Green Supermix (Bio-Rad Laboratories). Expression values were normalized to the internal control Beta-actin (Actb ...
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bioRxiv - Cell Biology 2019Quote: ... Resulting cDNA is used in PCR reactions (15 μl) performed in 96-well format in triplicate contained 1X SYBR green reagent (BioRad iQ SYBR Green Supermix), 0.4 μM gene specific primers and 0.5 μl of first strand reaction product (diluted 1:2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the absorbance of each well was measured at a wavelength of 450 nm by a Model 680 microplate reader (Bio-Rad; Richmond, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... dimethyl sulfoxide (DMSO) was added and the absorbance of each well was measured at 490 nm using an ELISA microplate reader (Bio-Rad, Hercules, CA, USA) (33).
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bioRxiv - Cancer Biology 2024Quote: ... real-time qPCR was performed on a Bio-Rad CFX96 Touch Deep Well Real-Time PCR System using iTaq™ Universal SYBR® Green Supermix (Bio-Rad #1725125) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... such that a total of 2 μg protein in 20 μL of buffer was loaded into wells of a Mini-PROTEAN TGX Stain-Free gel (5-14%) (Bio-Rad, Mississauga, ON, Canada). Protein ladder (5 μL of Precision Plus Protein™ All Blue Prestained Protein Standards ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Forty µg denatured protein per well was resolved by 8-12% SDS- PAGE homemade gels in parallel with multi-colored molecular weight marker (Bio-Rad Laboratories, Cat.No.1610395). Gels were run at 100V for 1-2 h and transferred overnight at 8- 12V ...
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bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
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bioRxiv - Molecular Biology 2024Quote: ... The immunoprecipitated and input DNA were analyzed by Agarose gel electrophoresis as well as subjected to real-time PCR using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad cat#172-5271) in CFX connect Real-Time instrument (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... and transfected with Fluorescence Multiplex-Host Cell Reactivation (FM-HCR) reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad, #165-2670) using conditions 260 V ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Absorption was read at 450 nm with wavelength correction set to 540 nm using an ELISA plate reader (Bio-Rad, Hercules, CA, USA). Intra- and inter-assay coefficients of variation were <10% for all EIAs.
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bioRxiv - Microbiology 2021Quote: ... The bound dye in the wells was eluted with 33% acetic acid and the absorbance of the solution was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). To assess the individual bacterial population during growth ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated for 24 h in an incubator and the optical density was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). Growth inhibition percentage was calculated by using the formula:
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bioRxiv - Genomics 2022Quote: ... The new plate with droplets was sealed with a pierceable PCR foil at 180 °C for 5 seconds using PX1 PCR Plate Sealer (Bio-Rad, Cat. 1814000). The PCR reaction was then carried out using the C1000 Touch™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad, Hercules, CA). The result is the representative from one individual’s CD14+ samples and a total of 2 healthy individuals’ CD14+ monocytes were tested ...
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bioRxiv - Microbiology 2021Quote: ... 12 – 16 individual colonies from each condition were selected from the m-TSAYE plates and DNA was extracted using Bio-Rad InstaGeneTM matrix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol for DNA extraction from bacterial cells ...
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bioRxiv - Immunology 2021Quote: ... according to the instructions provided by the manufacturer and the plate were then read using a Luminex-based Bio-plex Multiplex system (Bio-Rad, Hercules, CA). Samples were diluted to 1:3 for PEG− 2 and LTB4 analysis and 1:20 for IL-8 measurements.
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bioRxiv - Microbiology 2020Quote: ... and OD600 were measured from bacterial cultures diluted 1:10 using a Synergy H1 plate reader (Bio-Tek, Winooski, WI) and a Bio-Rad spectrophotometer (Bio-Rad, Hercules, CA), respectively ...
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bioRxiv - Synthetic Biology 2022Quote: ... the plates were analyzed for luminescence signals using a ChemiDoc Imaging System and Image Lab 6.0.1 software (Bio-Rad Laboratories Inc., Hercules, USA). Exposure time was set to 5 sec ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB2 was detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted with the Nucleospin RNA Isolation Kit (Machery-Nagel) and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). TaqMan real-time qPCR assays were used for quantification of human IFNB1 (Hs01077958_s1) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted using the PicoPure RNA Isolation kit and cDNA synthesis was performed using the iScript cDNA synthesis kit (Bio-Rad). cDNA samples were amplified with SYBR Select mastermix (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined by the Bradford method using a Bio-Rad protein assay kit or Lowry’s assay with a DC protein assay kit (Bio-Rad Laboratories). TonB1 and DdvT were detected using the ECL Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced™ - Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated either from homogenized mosquitoes or infected cell supernatant using a Multisource RNA Miniprep Kit (AP-MN-MS-RNA-250, Axygen) and reverse transcribed to cDNAs using an iScript cDNA Synthesis Kit (1708890, Bio-Rad). Viral genomes were quantified by qPCR using iTaq Universal SYBR Green Supermix (1725121 ...
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bioRxiv - Microbiology 2021Quote: ... using the primer-probe mix from the 2019-nCoV RUO kit (IDT) with iTaq Universal Probes One-Step kit (Bio-Rad). The 2019-nCoV_N_Positive Control plasmid (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was reversely transcribed with 0.5–1 μg RNA using the iScript cDNA synthesis kit as described in the kit (Biorad, Hercules, CA). The gene expression of Il-6 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted using the RNeasy plus micro RNA extraction kit followed by reverse transcription using iScript cDNA synthesis Kit (Bio-Rad). Equal amounts of DNA and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using the NucleoSpin RNA Plus Kit (Machery-Nagel) followed by reverse-transcription with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1μg of total RNA was reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (iScript cDNA Synthesis Kit, Bio-Rad) according to the provided protocol ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) was synthesized using a Qscript kit (QuantaBio, MA) as per kit instructions in a T-100 (Bio-Rad) thermocycler.
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bioRxiv - Microbiology 2019Quote: ... Relative fluorescence was measured at 5-minute intervals for 2 hours in a CFX96 system (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... containing 2 ml of anion exchange resin AGMP-1M (100-200 mesh, chloride form) (BIO-RAD, 1411841). The resin was first cleansed three times with 10 ml 18.2Ω water followed by 7 ml 0.5N HNO3 ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 μl of gene-specific primer pair and 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad). The relative transcript abundance of each gene (normalized to rpl4 ...
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bioRxiv - Microbiology 2021Quote: ... fixed with methanol supplemented with 2% H2O2 and incubated with a biotinylated anti-RSV antibody (Bio-Rad) in PBS containing 1% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... ramp rate set at 2 °C/sec] was performed in a thermal cycler (Bio-Rad C1000 Touch). Droplets were quantified in a QX200 Droplet Reader (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: Cells were mounted on a 2% agarose pad containing resuspension medium using a gene frame (Bio-Rad). Cells were concentrated by centrifugation (3300g for 30 s ...