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Citations for Bio-Rad :
4851 - 4900 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of total RNA was converted into cDNA with iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and 50 ng of cDNA was used in each reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% agarose TBE gels and detected by trans-UV illumination using the Gel Doc XR+ (Bio-Rad) and Image Lab software (version 5.1) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from ∼ 1 μg of extracted RNA using an iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed for IFNB and IL-1β on the Applied Biosystems PRISM 7500 qPCR machine ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated with a 1:5,000 dilution of StrepTactin-horseradish peroxidase (HRP) conjugate (Bio-Rad), and 1:500 dilution of custom polyclonal antibodies (Cocalico Biologicals ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and qPCR was performed using the iTaqTM Universal SYBR® Green 1-Step Kit (BioRad 1725151) on a Bio-Rad CFX Connect Real-Time System ...
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bioRxiv - Cell Biology 2019Quote: ... blots were incubated with 1: 5000 dilution of HRP-conjugated anti-mouse/ rabbit IgG secondary antibodies (Bio-Rad) in 1% milk-TBST for another hour ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) (Bio-Rad, Hercules, CA) or anti-mouse IgG-HRP conjugate (1:3000 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was used to make cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). All primer pairs were designed and validated in-house for efficiency and specificity ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were rinsed and incubated with the fluorescent secondary antibodies StarBright Blue 700 anti-mouse (1:5.000, BioRad) and StarBright Blue 700 anti-rabbit (1:5.000 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of extracted RNA was transcribed into cDNA using the iScript Reverse Transcription Supermix kit (Biorad, 1708840). Quantitative RT-PCR was performed using the PerfeCTa SYBR Green SuperMix (Quantabio ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Tween 20) at room temperature for 1 hour and stained with 0.3 μg/mL streptavidin-HRP (BioRad) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Secondary antibody used were an HRP-conjugated goat anti-mouse or rabbit at 1:25,000 (BioRad, Hercules, CA).
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bioRxiv - Genetics 2019Quote: ... followed by an anti-mouse (Abliance; 1/1,000) secondary antibody and then the Clarity Western ECL reagent (BioRad). Densitometric analysis was performed on non-saturated signals using the Image Lab™ software (BioRad).
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bioRxiv - Physiology 2019Quote: ... containing the pre-formed Cas9/sgRNA ribonucleoproteins and loaded into a 1-mm electroporation cuvette (BioRad, 165-2089). Electroporation was performed using the GenePulser Xcell (BioRad ...
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bioRxiv - Bioengineering 2019Quote: ... PAGE separation was carried out in 1 mm vertical mini gel format (Bio-Rad Mini-PROTEAN Tetra Cell) with a discontinuous buffer system under denaturing conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was generated from up to 1 μg of RNA with the iScript cDNA Synthesis kit (Bio-Rad). Quantitative PCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µL input and the entire IP fractions were reverse transcribed using the iScript cDNA synthesis kit (BioRad) and subjected to RT-qPCR ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were resolved on 1% agarose gels and imaged by ChemiDoc XRS+ system (Bio-Rad Laboratories). Image Lab™ Software 6.0.1 (BioRad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (SYBR® Premix Ex Taq™ ...
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bioRxiv - Physiology 2019Quote: ... Samples were then diluted to a final concentration of 1 μg/μl in 4x Laemmli buffer (Bio-Rad) and ultra-pure water and boiled for 10 minutes to denature proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 hr using a Trans–Blot device for semi-dry transfer (Bio-Rad Laboratories, Hercules, CA, USA). Membranes were then blocked for 1 hr at RT with 5% skim milk in 0,1 % PBS-Tween and incubated overnight at 4o with the following primary antibodies diluted in 5% milk ...
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bioRxiv - Genetics 2020Quote: ... The samples were then transferred at 0.25 A for 1 hour to a PVDF Membrane (Bio-Rad 1620177). Following protein transfer ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was prepared from 1 μg of RNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). cDNA samples were diluted 1:10 and real-time PCR was performed using IQ SYBR Green supermix and gene-specific primers in the iCycler real-time PCR detection system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using iScript Reverse Transcription Supermix (1708840, Bio-Rad). Quantitative PCR was performed with 100 ng of cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was obtained by retrotranscription of 1 μg of total mRNA with iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Reactions were then analysed by 20% denaturing polyacrylamide gel electrophoresis (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Systems Biology 2021Quote: ... Denatured cellular extracts equivalent to 1/120 OD600 were loaded onto a 7.5% precast Mini-Protean TGX (BioRad) and resolved by SDS-PAGE ...
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bioRxiv - Physiology 2020Quote: ... exposed to UV for 1 min and total protein patterns were visualized using ChemiDoc MP Imager (Bio-Rad). The stain-free gel contains a trihalo compound which reacts with proteins during separation ...
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bioRxiv - Molecular Biology 2020Quote: First-strand cDNA was synthesized using 1 μg of RNA with the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of total RNA was used to synthesize cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad). Real-time RT-PCR was performed using gene specific primers (Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... and an extension at 1 °C in a Bio-RAD T100™ Thermal Cycler (Bio-RAD, Hercules, CA). PCR amplicons from each reaction were purified using a QIAquick PCR Purification kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.2-1 µg of RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad). Real-time PCR assay was conducted using the SYBR Premix ExTaq II (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription and qPCR were performed using the iTaq Universal SYBR Green 1-Step Reaction Mix (BioRad 1725151) on a Bio-Rad CFX Connect Real-Time System ...
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bioRxiv - Plant Biology 2022Quote: ... 10-day-old seedlings true leaves of Arabidopsis lines were bombarded with 1 nm gold particles (Bio-Rad) coated with plasmid DNA ...
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bioRxiv - Genomics 2022Quote: ... Obtained cDNA was diluted 1:10 and subjected to qPCR analysis in triplicates using iQ™ SuperMix (BioRad). Viral mRNA expression was measured with HIV-1 gag primer probe mix (Supplementary Table 9 ...
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bioRxiv - Microbiology 2022Quote: ... for 1 hr and then counted using either an easyCyte 12HT or a ZE5 flow cytometer (Bio-Rad). Prochlorococcus cell sizes were normalized to 2 µm beads (Polysciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was generated from 1 µg of RNA using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad). Real-time quantitative PCR was performed on a StepOnePlus real-time PCT Machine (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein pellet was allowed to dry on air and was dissolved in 1 × Laemmli sample buffer (Bio-rad). Samples were heated at 70 °C for 10 min and 20 μg of protein for each sample was loaded onto a 1.5 mm 12% polyacrylamide gel ...
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bioRxiv - Neuroscience 2021Quote: ... HRP detection was performed by adding 1 mL Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
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bioRxiv - Biophysics 2020Quote: ... a kind gift from Julian Eaton-Rye) with anti-rabbit HRP-conjugated goat antibody (1:3000, Bio-Rad) as a secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... was used to purify the C-terminal 6X-His EfgA with 1 mL Ni-NTA columns (Bio-Rad). Columns were equilibrated with 10 mL of Buffer A at 1 mL/min prior to loading lysates ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:15000 in blocking buffer) followed by a goat anti-rabbit-HRP conjugate (170-6515, Bio-Rad, Hercules ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) (Bio-Rad, Hercules, CA) or anti-mouse IgG-HRP conjugate (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal total protein concentration were heated at 90°C in 1 × loading buffer (Bio-Rad 1610747) for ten minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: Western Blot samples of unbound fractions and IP were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Neuroscience 2022Quote: ... horseradish peroxidase (HRP) conjugated secondary antibodies (1: 5000) and signal detected using ClarityTM Western ECL Substrate solution (BioRad). Blots were visualised on a ChermiDOC XRS+ system (BioRad ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Immunoreactive bands were visualized using horseradish peroxidase-conjugated goat anti-rabbit IgG (Bio-Rad #170-6515; 1:3000), followed by ECL detection (GE Life Sciences) ...