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Citations for Bio-Rad :
4751 - 4800 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was retro-transcribed using the iScript cDNA Synthesis Kit and polydT primers (Biorad). The cDNA was amplified using the primers listed in S2 Table and the SsoAdvanced universal SYBR Green supermix (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse (#176516; 1:10,000 for Western blot) was from Bio-Rad. Alexa Fluor-conjugated goat antibodies against mouse IgG (1:500 for IF ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: A 1 mL Ni-NTA resin in a glass gravity-flow column (Econo-Column®, BioRad, USA) was set up inside a Coy anaerobic glovebox with an atmosphere of 100% N2 ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at room temperature and developed with Clarity™ Western ECL substrate (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2021Quote: ... Quantitative Real-Time PCR (qRT-PCR) was carried out using SYBR green 1 chemistry (BioRad, Hercules, CA). Samples for qRT-PCR experiments were assayed in triplicate using the BioRad CFX96 Q-PCR machine ...
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bioRxiv - Bioengineering 2020Quote: ... we prepared a 1% w/v agarose solution (molecular biology grade, Bio-Rad Laboratories, Hercules, CA, USA) in PBS under boiling until complete dissolution ...
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bioRxiv - Cell Biology 2020Quote: ... by wet electroblotting for 1 h at 100 V using the Mini-PROTEAN Tetra system (Bio-Rad). Equal protein loading was assessed by amido black staining (0.02% (m/v ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant contained 2.5 mg proteins/ 1 ml (determined using Bio-Rad Protein Assay, Cat. No. 5000006). This protein fraction was used for co-immunoprecipitation (input) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 107 cells were electroporated (Gene Pulser Xcell System, Bio-Rad: 250 V, 500 mF, 0.4cm cuvettes) with the following plasmid vectors ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed before incubation with 1:2,500 goat anti-mouse StarBright™ Blue 700 (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... The αM and β2 subunits of recombinant Mac-1 integrin were resolved on 4-20% polyacrylamide (BioRad), gradient gels by SDS-PAGE and stained with Coomassie brilliant blue R250 ...
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bioRxiv - Biochemistry 2022Quote: ... gels were dried at 80 °C for 1 h in a gel dryer (Model 583, Bio-Rad), exposed to phosphorimager plates and signal acquired in Typhoon Trio™ Variable Mode Imager (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... and then with horseradish peroxidase–conjugated Goat Anti-Rabbit IgG antibodies (1:15000 dilution, Bio-Rad, 1706515) or Goat Anti-Mouse antibody (1:15000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 µg of total RNA was converted to cDNA using iScript Select cDNA Synthesis kit (Bio-Rad). Quantitative RT-PCR was performed in triplicate using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript cDNA synthesis kit (Bio-Rad). Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and specified primers (Table 1) on a CFX96™ Real-time-PCR System (Bio-Rad Laboratories, USA). The thermal profile used for RT-qPCR was 95 °C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... HRP signals were visualised by enhanced chemiluminescence (ECL) (Cytiva) (1:25000) and imaged with Chemidoc (Bio-Rad). Primary antibodies targeting GAPDH (Chemicon ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was prepared from 1 ug of purified RNA using the iScript cDNA Synthesis Kit (Bio-Rad) as stated in the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... we mounted embryos individually in a column of 1% (w/v) low-melt agarose (Bio-Rad 1613112) in distilled water ...
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bioRxiv - Molecular Biology 2020Quote: ... 1/30 cDNA was applied for quantitative PCR reaction using SsoAdvanced Universal SYBR green supermix (Bio-Rad). Primer sequences are listed in the ‘Syber primers for enhancer RNAs (eRNAs ...
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bioRxiv - Physiology 2021Quote: ... 1 µg of total RNA was converted into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad). RT-qPCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA synthesis was performed with 1 μg of total RNA using iScript cDNA Synthesis Kit (Bio-Rad) and diluted 10-fold ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 was concentrated to 1-mL and isocratically eluted from an ENrich SEC650 10×300 column (BioRad) equilibrated with Buffer B ...
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bioRxiv - Genetics 2022Quote: ... The conidia were electroporated using 1 mm gap cuvettes (BIO-RAD Gene Pulser ®/ MicroPulser™ Cuvette) (Bio-rad ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated using 1 μg of RNA and the iScript cDNA Synthesis Kit (Bio-Rad, #1708891). qPCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μl of the protein extract was used to carry out a Bradford (Bio-Rad, 500-0205) assay ...
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bioRxiv - Genetics 2019Quote: ... at 400 mA for 1 hr on a Trans-blot SD semi-dry transfer cell (Bio-Rad). The HSF-1-HSE DNA complexes were visualized by autoradiography using LightShift Chemiluminescent EMSA kit (Thermo Fisher).
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bioRxiv - Biochemistry 2019Quote: ... and for AAC1 the primary antibody was 1:5000 dilution of rabbit IgG anti-AAC1 (Bio-Rad). The secondary antibody was 1:5000 dilution of horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2019Quote: ... Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891). Intron-spanning primers and probes for cep290 and ef1a1l1 were designed with Primer3Plus (http://primer3plus.com) ...
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bioRxiv - Pathology 2020Quote: ... and specific primers (Table 1) were performed using a CFX Connect Real-Time PCR Detection System (BioRad). Relative expression of mRNAs was determined using the software CFX Maestro (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was generated from 1 μg total RNA using the iScript cDNA Synthesis Kit (Bio-Rad). Assays were performed with PerfeCTa SYBR Green SuperMix ...
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bioRxiv - Plant Biology 2021Quote: ... 1-2μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis kit (BioRad) or StarScript II First-strand cDNA Synthesis Kit (GeneStar) ...
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bioRxiv - Genomics 2021Quote: ... before in situ reverse transcription and annealing of barcode 1+linker on a thermocycler (Bio-Rad T100). After RT ...
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bioRxiv - Immunology 2021Quote: ... cDNA was diluted 1 μg / 100 ul and qPCR was performed on a CFX96 Instrument (Bio-Rad) using SYBR Green incorporation ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 1 μg RNA using the iScript Advanced cDNA Synthesis Kit (Bio-Rad, #1725037). For the 3-12 mo trial ...
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bioRxiv - Physiology 2020Quote: ... but on a 10% Mini-Protean® TGX™ Precast Protein Gel (Supplemental Figure 1, BioRad, 4561036). Because of its limited use ...
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bioRxiv - Cell Biology 2021Quote: Mouse monoclonal anti-Viral V5-TAG antibody (1:5000; clone SV5-Pk1) was purchased from Bio-Rad/AbD Serotec ...
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bioRxiv - Molecular Biology 2021Quote: 1 μg Turbo DNase-treated RNA was reverse transcribed with the iScript cDNA synthesis kit (BioRad, 1708890), treated with 0.5 μL RNase cocktail (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... followed by streptavidin-alkaline phosphatase (1 µg/mL) and development with AP conjugate substrate kit (Bio-Rad). For analysis of intracellular cytokine production ...
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bioRxiv - Immunology 2021Quote: ... Expression of target and housekeeping genes (see Table 1) was analysed on CFXTM Manager (Bio-Rad Laboratories) using the “Gene Study” function ...
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bioRxiv - Microbiology 2021Quote: ... each cDNA was combined with segment-specific primers (Supplementary Table 1) and Precision melt supermix (Bio-Rad) and analysed by qPCR using a CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and transferred for 1 hour at 15V using Trans-Blot® SD Semi-Dry Transfer Cell (BioRad). The PVDF membrane was blocked with 5% milk in Tris-buffered saline with 0.1% tween-20 (TBST ...
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bioRxiv - Developmental Biology 2022Quote: 1 µg of RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Cat# 1708891, Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using iScript reverse transcription supermix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 ug of RNA using the iScript cDNA Synthesis Kit (BioRad #170-8891). Reverse Transcriptase Quantitative PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... Each column then received 200 µL of ProteOn 1 M Ethanolamine hydrochloride pH 8.0 (BioRad, Cat. # 1762450) and was incubated for 4 hours to quench any remaining active site on the resin ...
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bioRxiv - Genomics 2022Quote: ... 20 µg total protein extract per sample were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 1 h at 80 °C on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...