Labshake search
Citations for Bio-Rad :
4951 - 5000 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: Reaction products were analysed using 20% denaturing polyacrylamide gel electrophoresis (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) containing 7 M urea (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: 1 μg TURBO DNase-treated RNA was reverse transcribed with the iScript cDNA reverse transcription kit (Biorad, 1708890) or 5 U AMV-RT (NEB ...
-
bioRxiv - Genetics 2023Quote: ... cDNA was synthesized from 1 µg RNA with random hexamers using the iScript Reverse Transcription Supermix (Bio-Rad). Quantitative PCR was performed on a MyiQ (Bio-Rad ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The washed plugs were loaded in 1% Certified Megabase Agarose (2.4g agarose/240mL 0.5x TBE) (Bio-Rad, #1613108). CHEF-gel electrophoresis was performed on a CHEF Mapper System (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 μg of extracted RNA was transcribed into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad, 1708840). qPCR was performed using the PerfeCTa SYBR Green SuperMix (Quantabio ...
-
bioRxiv - Developmental Biology 2023Quote: ... Immunostaining was performed overnight at 4° C using primary antibodies for PTH (1:400, Bio-Rad, Hercules, CA), Cleaved caspase 3 (1:200 ...
-
bioRxiv - Microbiology 2023Quote: ... intermedius overnight cultures were pelleted and resuspended in a 10:1 ratio of culture to InstaGene Matrix (BioRad) after which the extraction protocol as per manufacturer instructions was followed ...
-
bioRxiv - Immunology 2023Quote: ... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
-
bioRxiv - Developmental Biology 2022Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000 dilution, Bio-Rad #1706515) or anti-mouse IgG-HRP conjugate (1:3000 dilution ...
-
bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated with secondary goat-anti-rabbit IgG (Bio-Rad Laboratories, CA, USA; dilution 1:3000) or goat anti-mouse IgG (Bio-Rad ...
-
bioRxiv - Genomics 2023Quote: ... and appropriate volumes of the hepatocyte suspension were embedded in 1% agarose blocks using disposable plug molds (BioRad). Hepatocytes were thoroughly lysed in-agarose using 1% lithium dodecyl sulfate in notothenioid ringer (100 mM sodium phosphate buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA was reversely transcribed from 1 µg of RNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891BUN) according to the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2023Quote: ... The following secondary antibodies were used: goat anti-rabbit IgG HRP conjugate (Bio-Rad, 170-6515, 1:5000) and goat anti-mouse IgG HRP conjugate (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Gels were incubated in 10% EtOH for 1 hr before imaging fluorescence on a ChemiDoc instrument (Bio-Rad).
-
bioRxiv - Microbiology 2023Quote: ... which included 1 x QX200™ ddPCR™ Evagreen® Supermix (Bio-Rad, CA, USA; catalogue number 1864033), 100 nM of the forward and reverse primers ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
-
bioRxiv - Neuroscience 2023Quote: ... and 1 μg of RNA was reverse-transcribed (RT) to cDNA using iScript cDNA synthesis kit (Bio-Rad) with oligo-dT primers for poly-A mRNA selection ...
-
bioRxiv - Genetics 2023Quote: ... 1 µg of total RNA was reverse transcribed to cDNA by using an iScript kit (#1708891, Bio-Rad) on a Mastercycler (Eppendorf) ...
-
bioRxiv - Immunology 2023Quote: ... by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf) on a real time thermal cycler CFX96 (Biorad).
-
bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Neuroscience 2023Quote: ... and Bio-Plex Pro Human Inflammation Panel 1 (37-plex) (Cat. No. 171AL001M) from Bio-Rad (Hercules, CA).
-
bioRxiv - Immunology 2023Quote: ... protein samples in assay-specific buffers were prepared with 1-2X final concentration of Laemmli buffer (Bio-Rad) with 2.5% β-ME and immunoblotted as described below ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR products were run on a 1% agarose gel and imaged using a Gel Doc XR+ (Bio-Rad).
-
bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 µg of RNA per sample using the iScript cDNA Synthesis Kit (Bio-Rad) or iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... and transformed by electroporation using the Gene Pulser Xcell (1700 V, 25 µF, 200 Ω, 1 pulse, Biorad) in C2989 5alpha electrocompetent bacteria (NEB ...
-
bioRxiv - Biochemistry 2023Quote: ... 20 mM imidazole and 1 mM DTT) and were then transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
-
bioRxiv - Microbiology 2023Quote: ... diluted 1:5000 in TBE for 30 minutes while shaking and imaged on Gel Doc XR+(Bio-Rad). Full-length bands for at each time point were calculated by densitometry using Image Lab software (Bio-Rad) ...
-
bioRxiv - Microbiology 2024Quote: ... 10 or 100 ng of plasmid DNA was transferred to a 1 mm MicroPulser electroporation cuvette (Bio-Rad) and cells were then electroporated at 1.8 kV ...
-
bioRxiv - Neuroscience 2024Quote: ... and 1 µg of RNA was used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s guidelines ...
-
bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reversibly transcribed to cDNA through reverse transcriptase PCR.
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed thrice and incubated with appropriate secondary antibodies conjugated with HRP (Bio-rad; 1:3,000). Bands were detected by Western-Lightning Plus Enhanced Chemiluminescence (Perkin Elmer) ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight with the following primary antibodies: αWIPI2 1:3000 (mouse, Bio-Rad, Hercules, CA, MCA5780GA), αWIPI4/WDR45 1:2000 (rabbit ...
-
bioRxiv - Neuroscience 2024Quote: ... HRP detection was performed by adding 1 ml Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
-
bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reverse transcribed to cDNA ...
-
bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
-
bioRxiv - Plant Biology 2020Quote: ... Real-time RT-PCR was performed using gene specific primers (Supplementary Table 1) and SYBR Green Supermix (Bio-Rad) in a CFX96 Real-Time PCR system (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: ... Synthesis of complementary DNA (cDNA) was completed using 1 μg of RNA and the iScript cDNA synthesis kit (BioRad). Each qRT-PCR was performed as triplicate reactions with the following reaction mixture ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunostaining for the vitronectin receptor (VNR) was with anti-CD51/61 (clone 23C6, 1:400; Bio-Rad, Oxford, UK) and for cathepsin K with a rabbit polyclonal antibody (3368-100 ...
-
bioRxiv - Cell Biology 2020Quote: All Rab34 used in steady-state assays was treated with AG 1-X8 anion exchange resin (Bio-Rad, 1401441) immediately before use to remove bound GDP ...
-
bioRxiv - Genetics 2021Quote: ... Hydrolysis probe ddPCRs (20 μl volumes) consisted of 1 x ddPCR Supermix for probes without dUTP (Bio-Rad Laboratories), virus-specific primers (HHV-6B POL F and HHV-6B POL R ...
-
bioRxiv - Microbiology 2021Quote: ... and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat. # 1708890, Bio-Rad). Real-time qPCR was conducted using the iTaq™ Universal SYBR® GreenSupermix (Cat ...
-
bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
-
bioRxiv - Genetics 2022Quote: ... then 1-5ng added to a PCR mix containing 11 μl 2x ddPCR Supermix for Probe (no dUTP, BioRad), 1.1 μl 20x DMBT1 probe assay (FAM-labelled) ...
-
bioRxiv - Molecular Biology 2020Quote: ... at 100 V for 1 hr in 4°C pre-chilled 1X Tris-Glycine buffer (Bio-Rad 161-0734). Membranes were blocked for 1 hr at room temperature in blocking buffer (PBS (Gibco 14190-144) ...
-
bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the appropriate resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively with buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... Plugs were loaded on 0.8% agarose gel in 1×TAE buffer (Certified Megabase agarose, Bio-Rad, ref# 161-3109) and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: ... Transformation was done chemically with heat shock at 42 °C for 30 s or by electroporation with 0.1 cm gap cuvettes at 1.8 kV for 1 s (Gene Pulser, Bio-Rad), depending on the competent cells required ...
-
bioRxiv - Microbiology 2019Quote: ... The integrity of the purified DNA template was assessed by conventional agarose gel (1% [wt/vol] electrophoresis (Bio-Rad), and the purified DNA samples were stored at 4 °C until further use.
-
bioRxiv - Biochemistry 2020Quote: ... The reconstitution sample was nutated for 1 h at 4°C before addition of 0.2 g/mL SM2-BioBeads (BioRad), and the reconstitution sample was further nutated overnight at 4°C before removal of the biobeads ...