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Citations for Bio-Rad :
5101 - 5150 of 5853 citations for 6 Chloro 5 6 dihydroimidazo 1 5 a pyrido 3 2 e pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesized from 1 µg total input RNA using iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad 1725035). In a qPCR plate (Bio-Rad MLL9601) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed with TBST and incubated with StarBright Blue 700 Goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 hour ...
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bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was normalized to 1 mg/mL using 0.1% Azo buffer by the Bradford assay (Bio-Rad). Samples were reduced with 30 mM DTT for 1 h and subsequently alkylated with 30 mM iodoacetamide for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used were the following: HRP-conjugated goat anti-mouse IgG (1:10000, Bio-Rad, 170-6516); HRP-conjugated goat anti-rabbit IgG (1:10000 ...
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bioRxiv - Microbiology 2023Quote: ... in 1×TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo Transfer System (BioRad). Blocking was performed in a 5% solution of dry milk and 0.05% Tween 1×PBS (1×PBST ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug of RNA was diluted into 15 uL reverse transcription reactions using the iScript RT kit (Bio-Rad) and reactions were carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Axonal staining and myelination in the SLM were assessed with rat anti MBP (BIO-RAD, cat # MCA409S 1:500) and mouse anti-Neurofilament H (NF-H) ...
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bioRxiv - Physiology 2023Quote: After washing with TBS-T 0.1% membranes were incubated with goat anti-mouse (1:1000, Bio-Rad, Madrid, Spain), goat anti-rabbit (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized from 1 µg of RNA using the iScript™ Reverse Transcription kit (Bio-Rad, USA). qRT-PCR was performed using gene-specific primers for eef1a1l1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative Real rime PCR (RT-qPCR) analysis was performed on genes of interest (Supplementary Table 1) using SYBRGreen (BioRad). Sso Fast EvaGreen Supermix was performed to analyze gene expression on a BioRad CFX96 Real-Time System (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was diluted 1 in 10 and 4µl of diluted supernatant was added to 200µl of Bradford reagent (Bio-Rad) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Complementary DNA was then synthesized from 1 μg of total RNA using iScript cDNA Synthesis Kit (1708890, Bio-Rad) and Bio-Rad T100 thermocycler ...
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bioRxiv - Genomics 2023Quote: ... and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, #1708890). We used RT-qPCR to quantify MYC expression (forward primer ...
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bioRxiv - Genomics 2023Quote: ... For quantitative real-time PCR 20 ng cDNA and 1 pmol/μl primers were mixed with 2x SYBR (BioRad) in a total volume of 13 μl for each well ...
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bioRxiv - Microbiology 2023Quote: ... 1 minute extensions and a final hold temperature of 12 C using a C1000 Touch Thermal Cycler (BioRad #1851197). Samples were subsequently loaded into the QX200 Droplet Reader for quantification ...
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bioRxiv - Biochemistry 2023Quote: ... CCT was immunoprecipitated from the lysate by adding 4 µg of a CCT5 antibody (BioRAD MCA2178, 1:100 IP) and incubating for 30 minutes at 4°C ...
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bioRxiv - Physiology 2023Quote: ... and total RNA (1 μg) was reverse transcribed to cDNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, Mississauga, Canada). SYBR Green (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies (IgG(H+L)-HRP) were used at 1:10000 dilution for detection using a Versadoc system (Biorad).
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bioRxiv - Biophysics 2023Quote: ... 50 mM Tris pH 7.4 was added to quench the reaction and the samples were buffer exchanged into BLI buffer (20mM Tris pH 7.4, 100mM NaCl, 1% glycerol, and 0.03% DDM) using PD-10 columns (Bio-Rad). For enterotoxins ...
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bioRxiv - Developmental Biology 2023Quote: ... and aliquots were 1:50 in water prior to Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were then normalized to 1 mg/mL protein in 0.1% Azo prior to reduction with 30 mM DTT at 37C for 1 h and alkylation with 30 mM chloroacetamide for 45 min ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
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bioRxiv - Microbiology 2023Quote: ... diluted to 20 ng/µL in molecular grade water and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). Relative expression was calculated by ΔΔCt method to GapDH 66 ...
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bioRxiv - Cell Biology 2023Quote: ... Thawed lysates were run on 7.5% Acrylamide gels with a 29:1 ratio Acrylamide:Bis using a Protean Tetra system (Bio-Rad) and Precision Plus markers (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... An aliquot of the DNA was resolved on 1% agrose gel and visualized on ChemiDoc Touch Imaging System (Biorad) to determine the quality of the tagmented libraries ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked for 1hr in PBS with 1mg/ml and 0.1% triton and then stained overnight with anti-alpha Tubulin antibody (1:100, MCA78G, BioRad). Cells were then washed ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:10,000, 52200336 and 52200341, Kirkegaard & Perry Laboratories) and detected by enhanced chemiluminescence using a Chemidoc (BioRad) gel imaging system ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein concentration das measured of each eluate sample in a 1:20 dilution by Bradford (BioRad protein assay). Per sample ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with the primary antibodies and developed with a horseradish peroxidase-conjugated goat anti-mouse (BioRad, 1706516, 1:5,000), anti-rabbit (BioRad ...
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bioRxiv - Plant Biology 2024Quote: ... The amplicons were separated in a 1% TAE agarose gel containing GelRed dye and imaged with ChemiDoc (Bio-Rad).
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bioRxiv - Developmental Biology 2024Quote: Table 1 for sequences) and qRT-PCR was done in duplicate using BioRad iQ SYBR Green Supermix (BioRad, 1708882) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s ...
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bioRxiv - Bioengineering 2024Quote: ... Sample concentrations were normalized to 1 ng/µL during cDNA preparation using the iScript Synthesis Kit (Bio-Rad, 1708841), following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes ...
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bioRxiv - Synthetic Biology 2024Quote: ... Signal intensities were quantified using the Quantity One 1-D Analysis Software (Version 4.6.6) (Bio-Rad, Hercules, CA, USA). sRNA signal intensities were normalized to the corresponding 5S rRNA signals.
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bioRxiv - Cell Biology 2024Quote: ... 1 tablet EDTA-free protease inhibitors) and protein content was determined using a Bradford protein assay (Bio-Rad, #5000001). 50-100 µg protein was run on an SDS-PAGE and blotted using a wet-transfer system (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg of total RNA was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories). Standard curves were run for all primer pairs to ensure high efficiencies (>97% ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then resuspended at a density of 1 × 107 cells/ml in Gene Pulser Electroporation Buffer (Bio-Rad). Aliquots of the cell/electroporation buffer mix were transferred into 1.5 ml microcentrifuge tubes (100 µl per condition) ...
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bioRxiv - Neuroscience 2024Quote: ... then 1 ml of “retentate” and “filtrate” were dot-blotted onto a nitrocellulose membrane (Bio-Rad Labs, Hercules, CA). Dot blots in which biotinylated Lec-bs was detected were performed as described below ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was prepared for western blotting by diluting 20 μg of protein with 1 × Laemmli buffer (Bio-Rad #1610747) and ultrapure water (Invitrogen #10977015 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined at indicated time points by adding 1/10 volume AlamarBlue solution (Biorad, Hercules, CA, United States) to each well ...
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bioRxiv - Cell Biology 2020Quote: ... and equal amounts (1–2µg) were used for reverse transcriptase reaction using an iScript™ Reverse Transcription Supermix (Bio-Rad). qPCR was run using the SYBR® Green Master Mix on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-encapsulated with Drop-Seq beads (ChemGenes: lot 083117) in 1 nL droplets using 15000 mL/hr oil (QX200, Biorad), 4000 mL/hr cell suspension and 4000 mL/hr bead flow rates (droplet generation frequency 2 kHz) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Cell Biology 2020Quote: ... incubating with HRP conjugated secondary antibodies (1:5000) and developing with Clarity™ Western ECL blotting substrate (Bio-Rad, 1705061) and imaging with X-ray film ...
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bioRxiv - Genetics 2021Quote: ... Proteins were visualized using horseradish peroxidase-conjugated goat anti-rabbit secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706515) or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated with ECL Western Blotting Substrate (Thermo, 32106) for 1 minute prior to imaging with the ChemiDoc system (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...