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Citations for Bio-Rad :
4401 - 4450 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Blots were then reprobed using the primary anti-actin 20-33 antibody (MilliporeSigma #A5060; 1:10,000 dilution) and an HRP-linked goat anti-rabbit secondary antibody (Bio-Rad #172-1019).
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bioRxiv - Microbiology 2024Quote: ... the membranes were washed with 1× PBS and visualised using Pierce™ ECL Plus Western Blotting Substrate with a ChemiDocTM MP Imaging System (Bio-Rad).
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bioRxiv - Immunology 2024Quote: ... the SVF cell pellets were resuspended in 1 mL and cells were counted using an automated cell counter (Bio-Rad TC-10).
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were prepared at a concentration of 1 μg/μL and 15 μL of sample was loaded onto 4-15% gradient SDS-polyacrylamide gels (Bio-Rad Laboratories). Following the electrotransfer of proteins onto PVDF membranes ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 6% milk in PBS and subsequently stained with mouse anti-V5 (clone SV5-Pk1, BioRad, 1:10 000) and goat anti-mouse (1:100 000 ...
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bioRxiv - Neuroscience 2024Quote: ... slices were bombarded with biolistic particles created by coating 1.6 μm gold particles (Bio-Rad, #165-2262; ∼1 μg DNA/mg gold) with constructs in mammalian expression plasmids ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesised from 1 μg of total RNA from each sample following the manufacturer’s instructions of the iScript™ cDNA synthesis kit (Biorad, California, USA). For RNA-Seq validation by RT-qPCR ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA was converted into cDNA using an iScript™ gDNA Clear cDNA Synthesis Kit (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: Protein concentration of the cell extracts was determined using the Bradford-Lowry method (Quick Start™ Bradford Protein Assay Kit 1, Bio-Rad). For western blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... BPH-1 cell viability was assessed using Trypan Blue staining quantified on a Biorad TC 20 (Bio-Rad, Marnes-la-Coquette, France) after 4 days of treatment with appropriate drugs.
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bioRxiv - Biochemistry 2024Quote: ... The reactions were initiated by adding ATP to a final concentration of 1 mM and incubated in a thermocycler (Bio-Rad T100) at 30°C for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then washed with TBS-T followed by exposure to the appropriate horseradish peroxidase-conjugated secondary antibody for 1 hr at room temperature and visualized on X-ray film using the enhanced chemiluminescence (ECL) detection system (Bio-Rad Laboratories) or with ChemiDoc Imager followed by acquisition with Image Lab Software (Version 5.2.1).
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bioRxiv - Cell Biology 2024Quote: ... 60°C 1 min]) was then performed using a CFX 384 Touch Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA). Relative quantification was performed using the calibration curve method ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting cDNA was diluted to 20ng/ul and mixed at 1:10 ratio with the SsoAdvanced Universal SYBR Green Supermix (BIO-RAD, 1725271). The qRT-PCR reactions were performed using the CFX96 Real-Time PCR System [C-1000 Touch Thermal Cycler] (BIO-RAD ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with fresh serum-free medium containing 10% CCK-8 for 1 hour at 37°C and then the absorbances at 450 nm were measured using a microplate reader (Bio-Rad, Hercules, USA).
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg DNA from each sample was transferred to a nitrocellulose membrane using the Bio-Dot SF microfiltration apparatus (Bio-Rad, #170-6542). The membrane was blocked in 5% non-fat milk in TBS with 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... The blot was then incubated with HRP-conjugated anti-His monoclonal antibody (1:5000) and detected with supersignalfemto substrate (Thermoscientific, USA) using ChemiDoc imaging system (Bio-Rad laboratories, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.08 of each M13 dye and 1 μL of the DNA template using a Bio-Rad thermocycler T100 (Bio-Rad, Pleasanton, CA, USA). PCR products were analyzed on an ABI 3500 genetic analyzer and sized against the LIZ500 internal standard (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... an affinity purified antibody generated against the SAX-7 cytoplasmic tail [gift of (Chen et al., 2001)] and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). For the loading control ...
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bioRxiv - Microbiology 2022Quote: ... and collecting 1 mL fractions from the top at a pace of 1 mL per minute while continuously measuring A254 using the BioLogic LP system (BioRad, Hercules, California, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
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bioRxiv - Immunology 2021Quote: ... Transcript expression was measured by qRT-PCR with the appropriate primers (Supplemental Table 1) using a Bio-Rad MyiQ™ Real-Time PCR Detection System (Bio-Rad Laboratories) to measure SYBR Green (IQ™ SYBR® Green Supermix ...
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bioRxiv - Neuroscience 2021Quote: ... PAI-1 and insulin) from plasma samples were analyzed by duplicate using the Bio-PlexPro mouse Diabetes group from Bio-Rad (Ref. 171F7001M) by Luminex® 200TM technology as previously described (Ortega Moreno et al. ...
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bioRxiv - Neuroscience 2020Quote: ... cys free or AAA SNAREs were reconstituted at a 1:400 protein:lipid ratio of the same lipid compositions by dialysis in the presence of Bio-Beads (Bio-Rad Laboratories, Hercules, CA) into metal-free buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
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bioRxiv - Plant Biology 2021Quote: ... The binary vector pMGE599 was introduced into Agrobacterium tumefaciens AGL-1 strain (62) through electroporation (E. coli Pulser; Bio-Rad, http://www.bio-rad.com). The scutella tissue of barley caryopsis was used for Agrobacterium-mediated transformation as described previously (63) ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription (RT) was performed with 1 μg of total RNA using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, 172-5272). cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% skimmed milk in TBST for 30 min at room temperature then incubated with primary antibodies overnight at 4°C and secondary antibody conjugated with horseradish peroxidase (HRP) for 1 h at room temperature before visualization by ECL Plus Western blotting detection reagent (Bio-Rad, Hercules, CA). Semi-quantification of the bands were performed using NIH Image J software.
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bioRxiv - Plant Biology 2022Quote: ... CLso mature effectors (without signal peptide) were amplified from haplotype A and B infected tomatoes genomic DNA under standard PCR conditions using iProof (Biorad, Supplementary Table 1). Annealing temperatures of primer pairs were calculated using FastPCR (Kalendar et al. ...
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bioRxiv - Immunology 2022Quote: ... The affinity matrix was resuspended in 150 μl PB for the unbound and full plasma fraction and in 40 μl PB with a 1% blocking buffer background (Bio-Rad, The Netherlands) for the bound fractions ...
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bioRxiv - Microbiology 2022Quote: ... were used as secondary antibodies for 1 hour at the room temperature and revealed with the addition of Clarity Western ECL reagent (Bio-Rad Laboratories Ltd). ChemiDoc MP Imaging System (Bio-Rad Laboratories Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... A second polyclonal antibody conjugated with alkaline phosphatase was used for immunodetection (1:1000) at wavelength 405 nm (BioRad Microplate Reader model 680) via the substrate p-nitrophenyl-phosphate ...
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bioRxiv - Neuroscience 2020Quote: ... plates were coated with 100 μl of either 1 μg/ml of rh-TfR1-ECD (purified in-house) or liver purified ferritin (Bio-Rad, 4420-4804) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and α-TUBULIN (1:4000) were detected using SuperSignal™ West Pico or West Femto Chemiluminescent Substrate chemiluminescence in ChemiDoc MP imaging system (BioRad, Berkeley, USA). The densitometry analyses were performed using Bio-Lab Software ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
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bioRxiv - Microbiology 2021Quote: ... The quality of extracted DNA samples was also checked by loading one μl of DNA in 1% Agarose Gel (Bio-Rad, California, USA). The extracts were stored at -20 C until use.
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bioRxiv - Developmental Biology 2020Quote: ... protein samples were boiled with β-mercaptol and ran in SDS-PAGE gels casted from 30% Acrylamide/Bis solution 29:1 (Bio-rad, 1610156) using the Mini-PROTEAN® system (Bio-rad) ...
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bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The total RNA (1 μg) from each sample was reverse transcribed into cDNA using the iScript cDNA select Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNAs were subjected to PCR analyses using specific primers (The primer sequences are listed in Table 1) on an amplification detection system (Bio-Rad, Hercules, USA). β-actin served as a housekeeping gene ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was diluted 1:40 in water before using 1 μL as template in 10 μL qPCRs with iTaq™ Universal SYBR Green Supermix (Catalog# 1725121, Bio-Rad, USA) and 400 nM each primer ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and OD600 were measured from bacterial cultures diluted 1:10 using a Synergy H1 plate reader (Bio-Tek, Winooski, WI) and a Bio-Rad spectrophotometer (Bio-Rad, Hercules, CA), respectively ...
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bioRxiv - Microbiology 2023Quote: The resulting amplicons and their corresponding lengths were visualized and documented via gel electrophoresis and analysis (Quantity 1 software, Bio-rad, CA, USA).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM DTT) to a final KOAc concentration of 75 mM and incubated with hydroxyapatite Bio gel HTP (Bio-Rad #130-0402) for 45 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue) were reverse transcribed to cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891) and stored at −20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...