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Citations for Bio-Rad :
4201 - 4250 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... was added to all samples and the products were separated on 15% polyacrylamide denaturing urea gels (19:1 acrylamide-bisacrylamide, Bio-Rad). The gels were fixed in fixing solution (40% methanol ...
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bioRxiv - Microbiology 2024Quote: ... and the RNA profile was measured by absorbance (254 nm) across the gradient in real time using an EM-1 Econo UV Monitor (Bio-Rad). All fractions with large polysomes ...
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bioRxiv - Microbiology 2024Quote: ... was added to the membrane and incubated for 1 minute at room temperature before imaging in the Image Lab software on a ChemiDoc MP Imaging System machine (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... diluted in 1% BSA-TBST (1:10,000) was used in combination with a goat anti-rabbit secondary antibody coupled with horseradish peroxidase (BioRad, 170-6515) diluted in 1% BSA-TBST (1:5,000) ...
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bioRxiv - Neuroscience 2024Quote: Microglia was assessed in brain sections using a monoclonal rat antibody against CD68 (1:200, MCA1957GA, Bio-Rad, Hercules, CA, USA) to label macrophage/microglia and a polyclonal rabbit antibody against Iba-1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed five times with PBS-T and incubated with HRP-coupled secondary antibody for 1 h at room temperature and imaged with the ChemiDoc Imaging System from Bio-Rad. Relative protein levels were quantified with Image Lab (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... Then membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit IgG-HRP, 1:10 000, Bio-Rad, # 170-6515 ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed then incubated for 1 hour at room temperature with secondary antibodies (StarBright blue 520 goat anti-mouse IgG and StarBright blue 700 goat anti-rabbit IgG, each at 1:2500, Bio-Rad) and tubulin (hFAB Rhodamine ...
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bioRxiv - Immunology 2024Quote: ... In the case of purified protein 1 μg protein was loaded per condition and the Precision Plus Dual Color protein marker (Bio-Rad) was used as size standard ...
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bioRxiv - Genetics 2024Quote: ... cDNA was diluted 1:4 with water and 2ul used as template for qPCR using 250nM primers using the SsoFast EvaGreen Supermix (BioRad 1725200) on an Applied Biosystems ViiA 7 Real Time PCR System ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples from the co-IP and were separated on 8% SDS-PAGE gels for 1 h at 120 V and transferred overnight at 20 V on to PVDF membranes (Bio-Rad). Blots were probed with rabbit anti-NMIIB/Myh10 (1:2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Physiology 2024Quote: ... were diluted in 2% BSA and membrane were incubated for overnight at 4°C followed by HRP-conjugated secondary anti-rabbit antibody (1:5000, cat# 1706515, Biorad, USA) incubation for 1hr at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Signal was developed with application of SuperSignal West Dura ECL substrate (Fisher, cat# PIA34075) (1 mL per blot) and visualized using a ChemiDoc XRS+ Imaging System (Bio-rad). Consistent exposure times were acquired for each sample set and probe ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was DNase treated and reverse transcribed using the iScript gDNA clear cDNA Synthesis kit (BioRad, 1725035). CDSs were then amplified with sequence specific primers annealing at the start (forward ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP/Input DNA samples were analysed using primer sets for specific genomic regions (Table 1) using iQ SYBR-Green Super mix (Bio-Rad) on a CFX384 Real-Time PCR system (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... The abundance of PtfoR in the input DNA (diluted 1:100) and output DNA was then quantified using quantitative PCR with iTaq Universal SYBR Green Supermix (Bio-Rad). A fold enrichment value was then generated by measuring the abundance of the tfoR promoter in the output (following immunoprecipitation ...
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bioRxiv - Bioengineering 2024Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Genomics 2024Quote: RT□qPCR was performed with a 1:10 dilution of cDNA using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, US) in a final reaction volume of 10 μL ...
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bioRxiv - Biochemistry 2024Quote: ... After 1-hour incubation at room temperature the reactions were terminated by addition of 4x Laemmli sample buffer (without β-mercaptoethanol) (BioRad), subjected to SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated for 1h with HRP-conjugated goat secondary antibodies against mouse or rabbit immunoglobulin (1:3000, 170-6516, 170-6515, Bio-Rad). Proteins were visualized on an iBright™ CL1000 Imaging System (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... First-strand cDNA was synthesized from 1 µg of total RNA using iScriptTM Reverse Transcription Supermix for RT-qPCR (Bio-Rad) and diluted 1:20 in Milli-Q H2O ...
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bioRxiv - Cell Biology 2024Quote: ... Electroporation was performed twice (1-minute interval) using 10 μg of each plasmid DNA and a Gene Pulser X cell electroporator (Bio-Rad) with a 2-mm gap cuvette ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected using Pierce ECL Western Blotting Substrate for 1 min before being imaged on a molecular imager (ChemiDoc XRS+, Bio-Rad) and analyzed by Image Lab.
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bioRxiv - Microbiology 2020Quote: ... This was followed by electroporation with 5 ms pulses of 1,350 V using the MicroPulserTM (BioRad). Cells were then resuspended in LB liquid (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Individual DNA was extracted in 200 μL 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA) [36] ...
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bioRxiv - Neuroscience 2021Quote: Probed cDNA amplification was conducted using 5 μL of SYBR Green Supermix (BioRad, Hercules, CA, USA), 0.5 μL primer ...
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bioRxiv - Molecular Biology 2020Quote: ... in which case it was blocked in 1XTBS-T with 5% milk (Bio-Rad 170-6404). The αMyc antibody was used at 1:3000 (Santa Cruz 9E10 c-myc) ...
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bioRxiv - Immunology 2021Quote: ... and within 5 minutes the plates were measured with a microplate reader (model 680; Bio-Rad) at 450 nm
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted in 250 μL 5% Chelex® 100 Resin (Bio-Rad Laboratories, Hercules, CA) and 3 μL of Proteinase K (20 mg/ mL ...
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bioRxiv - Cancer Biology 2021Quote: ... denatured at 95°C for 5 min and loaded onto Mini-PROTEAN TGX Gels (Bio-Rad). Samples were transferred to nitrocellulose membranes using the TransBlot Tubro system (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... then 5 µL was loaded onto an SDS-PAGE gel (Either 12% isocratic (Bio-Rad 4561046) or 4-15 % gradient acrylamide (Bio-Rad 4561086DC) ...
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bioRxiv - Plant Biology 2021Quote: ... for 5 ms was performed on the electroporation chamber using the MicropulserTM (Bio-Rad, #165-2100). 1 mL of liquid LB media was added and the bacteria were placed into a new tube and incubated at 29°C for 2-3h ...
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bioRxiv - Immunology 2020Quote: ... and proliferating cells stimulated for 5 days with α-CD3 (1µg/mL; clone UCHT1; Bio-Rad) and α-CD28/CD49d (each at 1 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... then heated at 90°C for 5 min before loading onto 4-20% gel (Bio-Rad). Proteins were separated using running buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with gentle rocking in 5% (w/v) Blocking-Grade Blocker (milk, Bio-Rad) in 1X Tris-buffered saline ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The total reaction volume contained 5 μl of iTaq Universal SYBR® Green supermix (BIO-RAD), 0.05 μl each of 10 μM of forward and reverse primers ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding sites were blocked with 5% fat-free dry milk (Bio-Rad Hercules, California) in PBS containing 0.1% Tween-20 (PBST ...
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bioRxiv - Immunology 2021Quote: ... at 5 μg/mL were incubated and coupled with Affi-gel Hz Hydrazide (Biorad #156-0016). Plasma 1:100 diluted from a patient with critical COVID-19 (Val 51 ...
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bioRxiv - Genetics 2022Quote: ... boiled for 5 minutes and resolved on 4 to 20% TGX mini protean gels (Bio-Rad). The proteins were transferred to PVDF membranes using BioRad Trans-Blot semi-dry transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were blocked with 5% nonfat dry milk (HA blot) or EveryBlot Blocking Buffer (Bio-Rad) (SREBP1 blot ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were heated at 95°C for 5 minutes and loaded on a 12% gel (BioRad). Gels were stained with Coomassie Blue ...
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bioRxiv - Physiology 2021Quote: ... into the lanes of a 5% stacking-and 10% separating polyacrylamide gel (Biorad, MiniProtean Tetra cell). The proteins were separated at 60 V for 30 min and 150 V until the Hb fraction (∼16 kDa ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol) 3 times and moved into a 25 mL Econo-Column Chromatography Column (Bio-Rad), where they were further washed with a minimum of 200 mL of wash buffer ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted using 250 μL of 5% Chelex 100 resin (Bio-Rad oratories, Hercules, CA) according to methods described previously (Hoffmann et al. ...
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bioRxiv - Cell Biology 2020Quote: ... boiled for 5 minutes and separated on precast 12% SDS-PAGE TGX gels (BioRad, 465-1044). Protein gels were transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... for 5 min and the signal was detected using ChemiDoc MP (Cat. # 170–8280, Bio-Rad). Quantification of band intensities was calculated using Image Lab 5.0 software (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reactions consisted of 5 μL of SsoAdvanced Universal SYBR® Green Supermix (BioRad, Hercules, CA, USA), 0.25 μL of forward and 0.25 μL of reverse primer at 10 μM ...