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Citations for Bio-Rad :
4351 - 4400 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Neuroscience 2024Quote: ... each RNA sample was reverse-transcribed and quantitative real-time PCR was performed simultaneously in a single step using iTaq Universal SYBR Green One-step kit (Bio-Rad, #1725150), in the Lightcycler instrument (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesised from 1 μg of total RNA from each sample following the manufacturer’s instructions of the iScript™ cDNA synthesis kit (Biorad, California, USA). For RNA-Seq validation by RT-qPCR ...
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bioRxiv - Physiology 2024Quote: ... and about 500 ng of total RNA was reverse transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). Real time (RT ...
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bioRxiv - Systems Biology 2024Quote: Protein concentration of the cell extracts was determined using the Bradford-Lowry method (Quick Start™ Bradford Protein Assay Kit 1, Bio-Rad). For western blotting ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was performed with SYBR green fluorescent PCR kit (Cat. # 208054) and ran on a CFX96 RealTime System (Bio-Rad Laboratories). The relative expression level was calculated as 2(-ΔΔCT) ...
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bioRxiv - Cell Biology 2024Quote: ... 1ug of RNA from each sample was then used for cDNA synthesis using the iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Real-time quantitative PCR was then performed in quadruplicate using a [check model] and detected using SYBR Green (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: RT-qPCR reactions of 12.5 ng DNase-treated RNA were prepared using the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) and run on the Bio-Rad CFX Opus 384 Real-Time PCR System ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA was converted into cDNA using an iScript™ gDNA Clear cDNA Synthesis Kit (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The SDS-PAGE gels were blotted onto PVDF membranes using the Trans-Blot Turbo Transfer System in combination with the Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit from Biorad and the SDS PAGE Transfer Buffer according to the manufacturer’s recommendation ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was developed with an ECL chemiluminescence detection kit and visualized with the Gel documentation system (Biorad ChemiDoc MP Imaging System). The protein level on the blot was quantified using ImageJ software.
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bioRxiv - Cancer Biology 2024Quote: ... About 1µg of total RNA was reverse transcribed to cDNA with the iScript cDNA Synthesis kit (Bio-Rad Laboratories, Hercules, CA; #1708891) following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X) on QuantStudio 5 Real-time PCR system (Bio-Rad, CA, USA) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from the cerebellum using the Aurum™ Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad, Hercules, CA, USA). Reverse transcription was performed using the iScript™ cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... DNA contamination in the treated RNA samples was assessed by performing a qPCR cycling with tilapia elongation factor 1α (EF-1α) primers using No-RT master mix (absence of reverse transcriptase enzyme provided in iScript™ Reverse Transcription kit, Bio-Rad, USA). The cDNA synthesis (20 µL reactions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The blood was first separated from the ethanol through gentle centrifugation and embedded in molten 2% agarose plugs (Bio-Rad DNA plug kit). The solidified plugs were submerged in Lysis Buffer solution (Bionano ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA synthesis and qRT-PCR was performed as described earlier (Neogy et al., 2019) using iScript cDNA synthesis kit and iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, India). Rice UBQ5-normalized ΔΔCt was used to calculate log2 fold change ...
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bioRxiv - Plant Biology 2020Quote: ... and the quantitative real-time polymerase chain reaction (qRT-PCR) was performed using the iScript™ One-Step RT-PCR Kit (Bio-Rad®), marking with SYBR® Green (Bio-Rad®) ...
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μg total RNA was reverse transcribed into cDNA in a reaction volume of 20 μl using the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad, 1725038). Real-time PCR reactions were performed on the resulting cDNA using the CFX Connect™ Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNA were used in cDNA synthesis with iScript™ Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, USA) while 700 ng of total RNA from the AT2 callus in LD and LL were used as an input ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the DC protein assay according to standard protocols provided by the kit manufacturer (Bio-Rad, Hercules, CA). Equal volumes of soluble protein supernatants were transferred to new tubes and subjected to in solution digestion ...
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bioRxiv - Cell Biology 2022Quote: ... The total amount of protein isolated from the weighed tissues was determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). Hydroxyproline content data are expressed as μg of collagen per mg of total lung protein (μg/mg).
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was transferred into clean tubes and the protein concentration was determined using the Bradford protein assay kit (Bio-Rad Laboratories, USA). The protein extracts (20 μg ...
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bioRxiv - Biochemistry 2021Quote: Reverse transcription (RT) was carried out with 500 ng of total RNA using the iScript™ cDNA Synthesis kit (Bio-Rad, Hercules, CA). qRT–PCR was carried out with the iQ™SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad, 172-5038). Quantitative real time-PCR was performed using Go-Taq qPCR Master Mix (Promega ...
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bioRxiv - Genetics 2022Quote: ... and cDNA was synthesized by with iScript™ cDNA synthesis kit according to the directions of the manufacturer (Bio-Rad, Hercules, CA, USA). Quantitative PCR was performed using Power SYBR™ Green PCR Master Mix kit on a StepOne Plus real time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Quality and quantity of retrieved RNA was assessed using the Experion automated electrophoresis system and a RNA StdSens Analysis kit (Bio-Rad Ltd., UK).
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bioRxiv - Microbiology 2020Quote: ... was used for quantification and all PCR reactions were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad Cat# 1864022). Primers used were previously described in (25) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Reverse transcription was performed with Onescript® cDNA synthesis kit (Abm) and qRT-PCR was performed using SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) and a CFX96™ Real-Time System device (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNase treatment was performed prior to cDNA synthesis with the iScript™ gDNA Clear cDNA Synthesis Kit (#1725035, Bio-Rad, Mississauga, ON, Canada). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... and 200 ng DNA were used to analyze KRAS codon 12/13 and 61 mutations with ddPCR KRAS G12/G13 and KRAS G61 Screening Kits and QuantaSoft Analysis Pro software (Bio-Rad, Hercules, CA) as described elsewhere63 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and visualised with Silver Stain Plus Kit (Bio-Rad Laboratories AB, Sweden) according to manufacturer’s modified silver stain protocol.
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were normalized for protein concentration using a BCA assay kit (Thermo 23225) and mixed with Laemmli buffer (Bio-Rad 161-0737). 50 µg of the protein mix were run through a 4-20% Criterion TGX gel (Bio-Rad 5671093 ...
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bioRxiv - Plant Biology 2021Quote: ... The protein concentration of each of the five biological replicates was estimated using a Bradford protein assay kit (Bio-Rad Laboratories, Hercules, USA).
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bioRxiv - Microbiology 2020Quote: ... the outer membrane control proteins OmpA (α-OmpA was kindly provided by Prasadaro Nemani (45)) or MOMP (α-MOMP, MONOFLUO™ Legionella pneumophila IFA Test Kit, BioRad), the inner membrane control protein LepB (antibody kindly provided by Gunnar von Heinje (46) ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration in supernatant will be determined by Bradford assay using the Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of total protein are separated on 8∼12% SDS–PAGE gels ...
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bioRxiv - Cell Biology 2021Quote: ... The total RNA (1 μg) from each sample was reverse transcribed into cDNA using the iScript cDNA select Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Thirty nanograms of cDNA per reaction were analyzed for semi-qPCR using the SsoAdvanced™ Universal SYBR® Green Supermix Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was used for various assays and the protein content of the homogenate was measured using DC protein assay kit (Bio-Rad, Hercules, CA). Lactase activity was assessed using a Lactase Assay Kit (Cat# E-BC-K131-S ...
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bioRxiv - Immunology 2021Quote: ... First-strand cDNA were synthesized from total RNA using an iScript gDNA Clear cDNA synthesis Kit according to the manufacturer’s instruction (Bio-Rad, Hercules, CA, USA). Real-time PCR was performed using the StepOnePlus Real-time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Total AGT concentration in each sample was normalized by total protein concentrations measured using a DC™ protein assay kit (Bio-Rad 5000112).
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bioRxiv - Immunology 2020Quote: ... 30-50µg of protein per lane alongside a prestained molecular weight protein marker was separated on an 8% SDS PAGE gel prepared from SDS-PAGE kit (Beyotime, China) and electrotransferred to immunoblot PVDF membrane (BIO-RAD, CA, USA) for protein blotting ...
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bioRxiv - Neuroscience 2021Quote: ... was used from each sample for One-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad) in the StepOnePlus™ Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... Results were validated using qPCR experiments where mRNA was reverse transcribed into cDNA using the iScript cDNA synthesis kit (cat #1708891, Bio-Rad, Hercules, CA). SYBR Green based qPCR was performed on a CFX-Connect Real Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed by using a HiScript II one-step qRT-PCR SYBR green kit (Vazyme) on a CFX96 real-time PCR detection system (Bio-Rad Laboratories, Inc.). The following primers were used ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. no. 1708890) or Superscript III (Invitrogen cat ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 0.2-1 µg of RNA was pre-treated with DNAse and used for cDNA synthesis using the iScript cDNA synthesis kit (Bio-Rad, Irvine, CA). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad, Cat. #1704275). Gels were activated by UV excitation using the Bio-Rad GelDoc XR+ System prior to transfer ...