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Citations for Bio-Rad :
4101 - 4150 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of each total RNA preparation as template and the iScriptTM Reverse Transcription Supermix for qRT-PCR Kit (Bio-Rad). Primers listed in supplemental table S7 were employed for RT-PCR and qRT-PCR analysis of NtGEF12 transcript levels in comparison to reference gene expression ...
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bioRxiv - Microbiology 2023Quote: ... 35 µg of lysate was loaded on 10% SDS-PAGE after quantifying the protein concentration using Bradford assay kit (Bio-Rad). After transferring bands to nitrocellulose membrane Ponceau S staining was performed for checking equal loading of protein on SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... blots were developed using a SuperSignal West Pico plus chemiluminescence substrate kit (Thermo) and imaged using a ChemiDoc MP imaging system (Bio-Rad). Images were analyzed using Image Lab (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR were performed following the manufacturer’s instructions for the iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad) using 100 ng of total RNA as template ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with QIAquick® PCR purification kit and 1-10 ng of immunoprecipitated or input DNA were amplified with iQ™ SYBR Green Supermix (BioRad) using a Real-Time PCR machine (BioRad IQ™ 5) ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNAs were prepared in a 10μl volume containing 350 ng of plant total RNA that was reverse-transcribed with an iScriptTM cDNA Synthesis Kit using modified MMLV-derived reverse transcriptase (Bio-Rad), a blend of oligo-d(T ...
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bioRxiv - Systems Biology 2023Quote: We performed cDNA synthesis with 0.5-1 μg of total purified RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from PMVECs and Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA) following the manufactureŕs instructions ...
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bioRxiv - Neuroscience 2022Quote: RNA isolation for all tissues were carried out using the Aurum Total RNA isolation kit (BioRad #732-6820; Hercules, CA, USA). Samples were reverse-transcribed to cDNA using qScript cDNA Supermix (Quantabio #101414-106 ...
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bioRxiv - Immunology 2022Quote: ... Separated proteins were transferred to a PVDF membrane using the Trans-Blot Turbo RTA Transfer Kit (Bio-Rad Laboratories, Feldkirchen, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from the purified RNA by both random and oligo(dT) priming using the iScript cDNA synthesis kit (Bio-rad). RNA levels were measured by the SYBR Green relative quantitation method using either a 7300 or Viia 7 RT-PCR machine (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 μg of RNA was used to synthesize cDNA with Bio-RAD iScript cDNA Synthesis Kit (170-8891, Bio-Rad). qRT-PCR was performed using Taqman universal PCR mix and predesigned gene expression assays with best coverage from Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA concentration was measured using a NanoDrop spectrophotometer and 1 µg of RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). Primers sequences were obtained from Harvard primerbank (sequences are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 3-4 μg of total RNA was reverse transcribed using the iScript advanced cDNA synthesis kit (Bio-Rad). Quantitative PCR of target genes (Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed to cDNA using the iScript™ gDNA Clear cDNA synthesis kit (Bio-RAD). qPCR was performed with Applied Biosystems Fast SYBR Green master mix on an Applied Biosystems QuantStudio 6 Flex system ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was carried out for 1 hour at 42°C using the iSCRIPT cDNA synthesis kit (Bio-Rad, Cat #1708841BUN) and 300 ng - 1 μg of RNA per reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were sonicated with a microtip for 5 sec at 20% amplitude and quantitated in DC Protein Assay kit II (BioRad, 5000112) against a BSA standard curve ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Genetics 2022Quote: Approximately 500 ng of total RNA was used to synthesize cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The HRM analysis was done according to the protocol provided by the Precision Melt Supermix Kit (Bio-Rad; Catalog #172-5112). The following oligonucleotides were used for the HRM analysis ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription of 500ng of total RNA was performed using the iScript cDNA synthesis kit (Bio-Rad Laboratories, Inc, California, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1µg of each total RNA sample was used to synthesize cDNA using the i-Script cDNA synthesis kit (Bio-Rad, USA). The real-time PCR was performed using a 20µl reaction volume on 96-well plates by using i-Taq Syber Green (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: Luminex beads used for the serological and purified antibody binding assays were prepared by covalent coupling of SARS-CoV-2 proteins with MagPlex beads using the manufacturer’s protocol with a Bio-Plex Amine Coupling Kit (Bio-Rad, France). Each of the SARS-CoV-2 Spike proteins expressed with different mutations were coupled with different colored MagPlex beads so that tests could be performed with a single protein bead per well or in a multiplexed Luminex binding assay ...
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bioRxiv - Zoology 2022Quote: ... sfRNA and 3’UTR were quantified together by RT-qPCR using the iTaq Universal Sybr green one-step kit (Bio-Rad) with primers previously designed [26] ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were separated in 10% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane using a Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit (Bio-Rad). Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Microbiology 2022Quote: Cell pellets from 2 mL overnight cultures were used to create gel plugs using the CHEF Bacterial Genomic DNA Plug Kit (Bio-rad). DNA was digested with I-SceI ...
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bioRxiv - Microbiology 2022Quote: ... β-lactamase activities were normalized relative to total protein concentration measured using Pierce Coomassie Plus Assay Kit (Bio-Rad, CA, USA) to obtain the enzyme activity in units pr ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We then performed quantitative polymerase chain reaction (qPCR) using 2 ng of complementary DNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quantity and quality was assessed using a NanoDrop spectrophotometer and cDNA was synthesized using an iScript cDNA Synthesis Kit (Biorad, 1708891) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (1 μg) was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170±8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated and purified from cultured cell lines using the Aurum Total RNA Mini kit (Bio-Rad, Cat# 7326820). RNA was converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: Whole cell and tissue protein was extracted using the Active Motif protein extraction Kit and quantified with the Bradford assay (Bio-Rad). Snap-frozen cardiac apical tissues were homogenized using Dounce tissue grinder (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... in SDS running buffer then blotted onto nitrocellulose membranes through Trans-Blot Turbo RTA Mini 0.2 µm Nitrocellulose Transfer Kit (Bio-Rad #1704270). Membranes were hybridized with 1:1000 dilution of the anti-Arm primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription to create complementary DNA (cDNA) with the iScript cDNA Synthesis Kit (Bio-Rad) according to manufacturer’s instructions in a total volume of 20 μl ...
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bioRxiv - Cell Biology 2023Quote: ... and cell death was performed using Annexin V-FITC/PI apoptosis detection kit following the protocol described by the manufacturer (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... The concentration of the isolated RNA was determined using Nanodrop and 250ng RNA was converted into cDNA using the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were developed using the ECL kit (Seven, Beijing, China) and detected using the ChemiDoc imaging system (Bio-Rad, California, USA).
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bioRxiv - Microbiology 2022Quote: ... The blots were then washed three times by PBST and then visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) by a ChemiDoc MP Imaging System (Bio-Rad).
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Complementary DNA (cDNA) was synthesized from RNA according to the iScript cDNA Synthesis Kit protocol (Bio-Rad Laboratories Inc.; Hercules, USA), and the concentration was determined using a Qubit flex fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... The following qRT-PCR thermocycling protocol was used with the iTaq™ universal SYBR® Green One-Step kit (Bio-Rad): 95 ºC for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 4995 µL of cell lysis buffer and 20 µL of PMSF 500 mM (Bio-Plex® Cell Lysis Kit, Biorad, #171304011). The lysate was frozen at −80°C for 2 minutes and then ...
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bioRxiv - Immunology 2023Quote: ... we developed a digital droplet RT-PCR (RT-ddPCR) employing the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad) and the QX200 Droplet Digital PCR System (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from whole Drosophila animals and converted to cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad, cat#1708891). Primers for qPCR were designed using IDT PrimerQuest (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and the protein concentrations in each tube were measured by Bradford assay using Quick Start Bradford protein assay kit (Bio-Rad). An equal amount of each sample (30-35 μl) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...