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Citations for Bio-Rad :
4051 - 4100 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... were resolved by SDS-PAGE in a 10 % v/v homemade polyacrylamide gel under reducing conditions and transferred to a nitrocellulose membrane by semi-dry transfer (kit #1704272, Bio-Rad). Membranes were saturated in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... One microgram of RNA per sample was reverse transcribed using the iScript™ Advanced cDNA Synthesis Kit (Bio-Rad, Hercules, CA) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: cDNA was synthesized by conversion of 1 µg of the DNAse-I treated RNA with iScript Selected cDNA Synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was first synthesised from the extracted RNA using the Bio-Rad iScript kit and a T100 Thermal Cycler (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was transferred to a clean new tube and protein concentration was quantified with the DC Protein Assay kit (Bio-Rad). SDS-PAGE and western blot analysis were conducted as described (Tseng et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 µg RNA (sorted cells) or 1 µg RNA (tissues) was used to prepare cDNA with the iScript™ cDNA synthesis kit (Biorad) with the following protocol ...
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bioRxiv - Neuroscience 2023Quote: ... analysis was performed by reverse transcribing 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). iQ quantitative PCR was performed on the diluted cDNA following the manufacturer’s protocol (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized in duplicate using 500 ng RNA in 10 uL iScript Advanced cDNA Synthesis Kit (Bio-Rad, 172-5038). Reactions were diluted 1:5 in water ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was collected and quantified using the Bio-Rad Protein Assay Kit and the Quick Start Bovine Serum Albumin Standard Set (Bio-Rad) and stored at −80°C before use.
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bioRxiv - Microbiology 2023Quote: ... The membranes were then rinsed with TBST as above before chemiluminescence detection using the Clarity Western ECL substrate kit (cat. no. 1705060, Bio-Rad). Gels and blots were recorded with a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... diluted to 1 ng/μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Pathology 2023Quote: ... The immune complexes were detected using a chemiluminescence kit and visualized via the ChemiDoc XRS Gel documentation system (Bio-rad, CA). The gray intensity was measured by the software of Quantity One (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 ng RNA (RIN>8) was transcribed to cDNA using the iScript first strand cDNA synthesis kit (Bio-Rad 1708891). qPCR was carried out on a 384-well Quantstudio Flex system (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using oligo-d(T) primers and avian reverse transcriptase (iScript cDNA Synthesis Kit, Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... mRNA levels of SASPs (Figure 1e) were measured by RT-qPCR using iTaq Universal One-Step RT-qPCR Kit (Bio-Rad) with primer pairs previously described 67 ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of 1000 ng of RNA was used for cDNA synthesis using a High Capacity cDNA Reverse Transcription Kit (BioRad, #1708891). To detect the contaminated DNA we used the samples processed without the transcriptase reverse enzyme as negative controls ...
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bioRxiv - Zoology 2022Quote: ... RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad). Primers for five genes—Clock ...
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bioRxiv - Plant Biology 2022Quote: ... The chemiluminescent signals were developed by WesternBright ECL kit (LPS Cat# K-12045-D20) and documented on the ChemiDoc MP system (Bio-rad).
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bioRxiv - Plant Biology 2022Quote: ... WesternBright ECL kit was used to detect the chemiluminescent signals (LPS Cat# K-12045-D20) and ChemiDoc MP system (Bio-rad) was used to capture the image.
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bioRxiv - Microbiology 2022Quote: ... 600 ng in 15 μL was used for reverse transcription using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). The reverse transcription program was run as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the cDNA was used with the KAPA SYBR FAST Universal qPCR Kit (KAPA) and run on a CFX96 real-time PCR detection system (Bio-Rad). The qPCR primers are listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1-1.5 µg RNA was used a template to make cDNA with the iScriptTM cDNA synthesis kit (Bio-rad #1708890), as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression of TRAF6 was determined relative to GAPDH using iTaq Universal SYBR Green One step RT-qPCR kit (Bio-Rad) employing the Ct method as described in Bio-Rad RT-qPCR user manual.
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat # 5000111; BioRAD, Inc, Hercules CA) with bovine serum albumin as a standard according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: RNA was extracted from about 2000 L4 animals and reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was then analyzed on the CFX384 thermocycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from mouse tissues using the Aurum Total RNA Fatty and Fibrous Tissue Extraction kit (Biorad: #732-6870). RNA from cells was extracted using Zymo Research R1055 Quick-RNA MiniPrep Kit ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by chemiluminescence using the Clarity Western ECL substrate detection kit (Bio- Rad) and a Chemidoc imaging system (Bio-rad). Whole proteins were detected on gel using the stain-free technology.
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of RNA was reverse transcribed to cDNA with the iScript cDNA Synthesis Kit following the manufacturer’s protocol (Bio-Rad, #1708891).
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bioRxiv - Genomics 2024Quote: cDNA corresponding to rRNA in both the large and small size cDNA fractions were removed using the SEQuoia kit (Biorad, 17006487) according to the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA synthesis was performed using 900 ng of total RNA for hPSCs and 1000 ng of total RNA for EBs and the iScript Reverse Transcription Kit (BIO-RAD) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Only RNA with RIN value above eight was used for cDNA synthesis using the iScript cDNA synthesis kit (Biorad, Cat.No.1708891). RT-qPCR was then performed in a 96-well plate using the PowerUp SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from about 3000 L4 animals and reverse transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). cDNA was then analyzed on the CFX384 thermocycler (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... blots were visualized using the LI-COR Odyssey CLx or the Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) and a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Distinct MagPlex microsphere sets (Luminex Corp, Austin, TX, USA) were respectively coupled to viral antigens using the amine coupling kit (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was collected and protein quantification was performed using the BCA assay (BioRad DC Protein assay kit, Bio-Rad 5000112). 1 mg of protein per condition was used for co-immunoprecipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The digestion products were analyzed by 12-15% SDS-PAGE and stained with Coomassie blue or silver (Silver stain plus kit, Bio-rad). Images were taken using a Chemidoc imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then exposed to the ECL substrate kit (Pierce 34850 and 34095) for 3 minutes before imaging with the ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total RNA was used for reverse transcription with the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR analysis was performed using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 1 μg of total RNA using the Reverse Transcription system (iScriptTM cDNA synthesis kit, Bio-Rad). Quantitative Real time PCR was performed using Sybr Green Master Mix (SYBR Green master mix ...
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bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... Custom primers were designed to meet several criteria using NCBI Primer Blast followed by running of the complementary DNA (cDNA) synthesis product made using RT-PCR (iScript kit, Cat no. 1708890, Biorad, USA) on an electrophoresis gel ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoreactivity signals were detected with the SuperSignal West Femto Maximum Sensitivity Substrate kit (Pierce) and a Chemico image system (Bio-Rad). Densitometry quantification of protein band intensities was carried out using ImageJ.
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bioRxiv - Physiology 2023Quote: ... diluted to 1 ng/ μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Cell Biology 2024Quote: ... RNA isolation was succeeded by cDNA preparation from 1µg of total RNA using iScript™ cDNA Synthesis Kit (#1708891; Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... All mRNAs were transcribed using the SP6 mMessage mMachine kit (Fisher Sci #AM1340) and purified using Biorad Microbiospin columns (Biorad #7326250). Two guide RNAs targeting chordin and Three guide RNAs targeting tyrosinase were transcribed using T7 RNA polymerase (NEB #M0251s ...
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bioRxiv - Cancer Biology 2024Quote: scATAC-seq libraries were performed with the Bio-Rad’s SureCell ATAC-Seq Library Prep Kit according to the manufacturer’s instructions (Bio-Rad, Cat# 17004620). Library concentration and fragment size were checked by Agilent 2100 Bioanalyzer ...