Labshake search
Citations for Bio-Rad :
4151 - 4200 of 4536 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... All lysates were normalized using bicinchoninic acid (BCA; Pierce BCA Protein Assay Kit) and combined with 4x Laemmli buffer (Bio-Rad). We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad ...
-
bioRxiv - Physiology 2023Quote: ... diluted to 1 ng/ μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
-
bioRxiv - Plant Biology 2024Quote: ... graminicola genomic DNA was quantified by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for the histone 3 (CgH3 ...
-
bioRxiv - Plant Biology 2024Quote: ... The rRNA depleted was used immediately to prepare the metatranscriptome libraries using the SEQuoia complete RNAseq kit (Bio-Rad cat# 17005726) following the manufacturer’s procedures without any modifications ...
-
bioRxiv - Plant Biology 2024Quote: ... higginsianum genomic DNA quantitation was performed by quantitative PCR using Biozym Blue S’Green qPCR Kit (Biozym Scientific, Germany) in a CFX RT-PCR detection system (Bio-Rad, USA) and specific primers for ChTrpC gene (Supplementary Table S1) ...
-
bioRxiv - Biophysics 2024Quote: ... A luminol-based chemiluminescence assay kit (Chemi-Lumi One L, Nacalai tesque) and chemiluminescence detection system (ChemiDoc Touch MP imaging system, Bio-Rad) were used to detect chemiluminescence signals.
-
Dynamic regions allosterically connect the USP14 active site with the proteasome interaction surfacebioRxiv - Biophysics 2024Quote: ... and the units were converted to molar ellipticity based on protein concentration measured in the sample by the DC protein assay kit (Bio-Rad) using WT USP14 as standard ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were then blotted onto polyvinylidene difluoride (PVDF) membranes using Trans-Blot Turbo™ Transfer Starter System and Midi PVDF kit (Both are from Biorad). After transfer ...
-
bioRxiv - Neuroscience 2024Quote: ... the gel was transferred to a LF PVDF membrane using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (BIO-RAD, 1704275). After blocking in 5% skim milk powder (Sigma Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: ... Lysates were heat at 95°C for 10 min and then separated by SDS-PAGE electrophoresis on 10% acrylamide gels (FastCast™ Acrylamide kit, BioRad) at 110V for 60 min ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
-
bioRxiv - Microbiology 2024Quote: RNA extracts were reverse transcribed into cDNA using an iScript™ Select cDNA Synthesis Kit (Bio-Rad, Marnes-la-Coquette, France) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCRs (qRT-PCRs) were carried out in triplicate with three technical replicates with the SsoAdvanced Universal SYBR Green kit (Bio-Rad) on a CFX384 Touch Real-Time PCR Detection System (BioRad) ...
-
bioRxiv - Microbiology 2024Quote: We carried out reverse transcription with RNA prepared for transcriptome analysis using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA product was diluted 10-fold with water and we used 1Lμl per technical replicate for quantitative PCR analysis ...
-
bioRxiv - Immunology 2024Quote: ... The following cytokines and chemokines were measured using a Bioplex Express Kit according to the manufacturer’s directions (Biorad, Hercules, CA, USA): GM-CSF ...
-
bioRxiv - Microbiology 2024Quote: ... A total of 500 ng of resulting total RNA was used to synthesize cDNA via random priming utilizing the iScript™ cDNA Synthesis Kit (Biorad). Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems ...
-
bioRxiv - Genetics 2024Quote: 500 ng of RNA was converted to cDNA using the iScript cDNA synthesis kit (#1708891, Bio-Rad, Hercules, CA, United States) containing random hexamers according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... RNA concentrations were measured with the Little Lunatic (Unchained Labs) and cDNA was synthesized using the iScript cDNA synthesis kit (Bio-rad). Primers for skeletal marker genes were designed using NCBI Primer-BLAST (Supplementary Table 3 ...
-
bioRxiv - Developmental Biology 2024Quote: 100 ng of enriched small RNA fraction was used for generated cDNA following iScript Select cDNA Synthesis Kit (#708896, Bio-Rad) following manufacture’s protocol and the specific primer 5′-GCAGGTCCAGTTTTTTTTTTTTTTTCTACCCC-3′ (Supp ...
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA for measuring primary-miR430 was generated using 200 ng of total purified RNA following iScript cDNA synthesis kit (#1708890, Bio-Rad) manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... Biotin signal was detected and quantified by chemiluminescence according to standard LightShift Chemiluminescent RNA EMSA Kit protocol using a ChemiDoc™ Touch Imaging System (BioRad).
-
bioRxiv - Genetics 2024Quote: ... and cDNA was generated from 0.5 micrograms of purified RNA from each sample using an iScript cDNA Synthesis Kit (Bio-Rad #1708890). qPCR was conducted using iTaq Universal SYBR Green Supermix (Bio-Rad #1725151) ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from cells using GeneJet RNA Purification kit (Thermo Fischer) and cDNA was synthesized using iScript Reverse Transcription Supermix (Bio-Rad), as previously described (27) ...
-
bioRxiv - Bioengineering 2024Quote: ... RNA was purified from the lysates and immediately converted into complementary DNA (cDNA) using a reverse transcription kit (iScript Reverse Transcription Supermix, Bio-Rad). The resulting cDNA samples were stored at -80°C until PCR analysis ...
-
bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was used for a reverse transcriptase reaction using iScript cDNA Synthesis Kit (Catalog #170-8891; Bio-Rad). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
-
bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µg of each RNA sample was used in a 20-µl reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S6 ...
-
bioRxiv - Microbiology 2024Quote: ... blots were developed using a SuperSignal Weser Pico plus chemiluminescence substrate kit (Thermo) and imaged on a ChemiDoc MP imaging system (Bio-Rad). Images were analyzed and bands quantified using Image Lab (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... isolated mitochondria either bound to HA-beads or extracted from whole tissues homogenates were evaluated for protein concentration using the DC Protein Assay kit (Bio-Rad). Specific amounts of mitochondria based on assayed substrates were then resuspended in 20 μL of mitochondrial isolation buffer and plated onto a 96-well plate ...
-
bioRxiv - Neuroscience 2024Quote: ... The cDNA was then amplified using the SYBR Premix (iScript Advanced cDNA Synthesis Kit) using the CFX96 Touch Real-Time PCR Detection System StepOnePlus (Bio-Rad). Briefly ...
-
bioRxiv - Microbiology 2024Quote: ... was performed using the diastarTM onestep multiplex qRT-PCR kit (Solgent, South Korea) and Bio-Rad CFX Maestro 2.3 system (Bio-Rad, USA) with the following specific primers ...
-
bioRxiv - Plant Biology 2024Quote: ... The biotin-labelled DNA probes were detected using the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoScientificTM; USA) and pictured under BioRad Gel DocTM XR (BioRad; USA).
-
Synchronous 3D patterning of diverse CNS progenitors generates motor neurons of broad axial identitybioRxiv - Neuroscience 2024Quote: ... 250- 500ng of RNA was reverse transcribed using the Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit in a C1000 Touch Thermal Cycler (Biorad), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... One or one-half microgram of total RNA was reverse-transcribed using iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad 1725035). If RNA was treated with TURBO DNase (Thermo Fisher AM2238) ...
-
bioRxiv - Microbiology 2024Quote: ... Binding to CHO cells was determined by digital droplet PCR (ddPCR) using the 2X ddPCR Supermix for Probes (No dUTP) kit (Bio-Rad) with primers targeting SINV nsp2 and Mmadhc47 (Supp ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membranes using the Trans-Blot Turbo RTA Midi 0.2 µm PVDF Transfer Kit (Bio-Rad, 1704273) and a Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Microbiology 2024Quote: ... Four hundred nanograms of purified RNA was reverse transcribed into cDNA following the manufacturer’s instructions for the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, #1725035). qRT-PCR was performed using the SsoAdvanced Universal SYBR Green Supermix (BioRad ...
-
bioRxiv - Microbiology 2024Quote: ... the blots were washed four times again by PBST and visualized using an Omni-ECL Femto Light Chemiluminescence Kit (EpiZyme, SQ201) through a ChemiDoc MP Imaging System (Bio-Rad). To examine the S glycoprotein packaging efficiency ...
-
bioRxiv - Plant Biology 2024Quote: ... 5 μl supernatant was loaded onto 10% SDS polyacrylamide gels and the proteins were transferred onto PVDF membranes using the Trans-Blot Turbo RTA Transfer Kit (1704275, Bio-rad). Membranes were blocked in PBS-T (tablets 524650-1EA ...
-
bioRxiv - Physiology 2024Quote: ... First-strand cDNA was synthesized with oligo (dT) primers and the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories, München, Germany). Gene expression was measured using the SsoFast EvaGreen Supermix on a CFX96 Real-Time PCR Detection System (both Bio-Rad Laboratories) ...
-
bioRxiv - Physiology 2024Quote: ... Protein concentration in the homogenates was determined using a commercial assay kit (Bio-Rad Protein Assay, Bio-Rad, Hercules, CA, USA). Na+/K+-ATPase and H+-ATPase activities were expressed as µmol ADP mg protein-1 h-1 and carbonic anhydrase activity was expressed as µmol H+ mg protein-1 min-1.
-
bioRxiv - Physiology 2024Quote: ... Detection used anti-rabbit or anti-mouse secondary antibodies linked to alkaline phosphatase (Jakson ImmunoResearch) and the alkaline phosphatase conjugate substrate kit (Bio-Rad). The ImageJ sofware analysis program (NIH ...
-
bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was carried out via an iProof SYBR Green Supermix kit and CFX96 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Reverse-transcription products of samples were amplified by the One-step gDNA Removal and cDNA Synthesis kit (TransGen Biotech) using the SYBR Green SuperMix (Bio-Rad). Primers used for RT-PCR assays are listed in Table S1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was DNase treated and reverse transcribed using the iScript gDNA clear cDNA Synthesis kit (BioRad, 1725035). CDSs were then amplified with sequence specific primers annealing at the start (forward ...
-
bioRxiv - Molecular Biology 2024Quote: ... The blots were visualized using an enhanced chemiluminescence detection kit and followed by ChemiDoc Touch Imaging System (Image Lab, Bio-Rad).
-
bioRxiv - Microbiology 2024Quote: ... RT-qPCR was conducted by the HiScript® II One Step qRT-PCR SYBR Green Kit (Q221-01, Vazyme, Nanjing, China) using the C1000 Touch Thermal Cycler/CFX96 Real-time system (Bio-Rad) and the data were analyzed using Bio-Rad CFX Maestro software (version 4.1.2433.1219) ...
-
bioRxiv - Physiology 2024Quote: ... Complementary DNA was synthesized from 0.5 μg of RNA using the iScript complementary DNA synthesis kit (Bio-Rad; catalog no.: 1708891). Real-time quantitative PCR was performed on a Bio-Rad CFX96 using SsoAdvanced Universal SYBR Green SuperMix (Bio-Rad ...
-
bioRxiv - Immunology 2024Quote: ... RT-qPCR was performed using a LightCycler® 96 and iTaq Universal Probes One-Step RT-qPCR kit (BioRad, Temse, Belgium), with purified RNA and primers and probes specific to SARS-CoV-2 as reported by Boudewijns et al ...
-
bioRxiv - Immunology 2024Quote: ... for two hours at RT with mild rotation and proteins in FhNEJ-Som were acetone-precipitated using the ReadyPrep 2-D Cleanup kit (BioRad, #1632130) according to the manufacturer’s instructions ...