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Citations for Bio-Rad :
4251 - 4300 of 7697 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... samples were solubilized in Laemmli sample buffer and heated at 95°C for 10 min to denature proteins and separated via SDS-PAGE for 40–60 min at 270 V using 4–20% stain-free gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Thereafter ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein lysates (25 µg) were separated in equal volume on 4 – 20% Mini-PROTEAN TGX™ Precast Protein Gels (Bio-Rad, cat. # 4561096) and transferred to an Immun-Blot polyvinylidene difluoride membrane (PVDF cat ...
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bioRxiv - Molecular Biology 2019Quote: ... unbound and eluted proteins were precipitated by TCA extraction before running on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad #4561096) and western blotting was performed as described.
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bioRxiv - Neuroscience 2021Quote: ... These samples were run on a 4–20% SDS-PAGE and electroblotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories, Hercules, CA, USA) from the gel by a semidry blotting apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Bioengineering 2021Quote: ... incubated for 5 min at 95°C and used as template in the qPCR reaction in a final 10−4 dilution with the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The qPCR was run in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Immunology 2020Quote: ... of protein were incubated in 1X Laemmli buffer with 0.1 M dithiothreitol at 65°C for 15 min and electrophoresed on 4-15% gradient TGX gels and transferred to PVDF membranes (Bio-Rad Laboratories, Mississauga, Canada). The membranes were blocked with Tris-buffered saline with 0.05% Tween 20 (TBST) ...
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bioRxiv - Biophysics 2020Quote: ... Pure ClyA-AS type-I (12-mer) nanopores were obtained using native PAGE on a 4-15% gradient gel (Bio-Rad, Veenendaal, The Netherlands) and subsequent excision of the correct oligomer band.
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from recovered fractions were analysed by Western blotting (4-15% Mini PROTEAN® TGX Stain-Free™ Gels & Trans-blot® Turbo™ Nitrocellulose Transfer Pack, BioRad). The bait proteins were probed using a His Tag HRP-conjugated antibody (Ozyme ...
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins or whole cell lysates were separated by SDS-polyacrylamide gel electrophoresis on a 4-20% gradient gel (Bio-Rad Laboratories, Hercules, CA) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts (50 μg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Biochemistry 2021Quote: ... Premade 4-20% TGX gels with either the 10-well 50 μL or 15-well 15 μL formats (Bio-Rad cat. # 4561094 and 4561096) were used in all instances ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 µg of each protein mixture was separated by SDS-PAGE (polyacrylamide gel electrophoresis) on a Criterion XT 4-12% Bis-Tris gel (Biorad Laboratories cat. No. 3450124). Proteins were transferred to a PVDF membrane (Merck Millipore cat ...
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad Mini-PROTEAN® TGX™ 4-15% Gel) according to manufacturer’s protocols for electrophoresis at 110 V for 70 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Bead pellets were resuspended and boiled in β-mercaptoethanol containing Laemmli sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2022Quote: ... The radio-labelled proteins were then separated with SDS-PAGE on a 4-12% Criterion XT Bis-Tris gels (Bio-Rad, Hercules, CA, USA) in MES buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins in the lysates were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis using gradient (4–20 %) PAGE gels (Bio-Rad, Mini-PROTEAN TGX), with a molecular mass marker (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... the indicated ESA supernatant was boiled in SDS-PAGE sample buffer and 3 μg of each sample was run with a 4-15% TGX gradient gel (Bio-Rad cat. no. 4561086), then transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2022Quote: ... 30μg protein for iPSC-derived optic cups or 15μg for iPSC-LESCs were loaded onto 4-15% Mini-PROTEAN ® TGX ™ gels (BioRad Inc., CA, USA) and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded onto 4-12% Criterion XT precast gels and proteins were separated by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electroblotted onto a 0.45 μm nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... by tank blotting at 250mA for 90 min in a cold room (4 °C) in blotting buffer (catalog#1610734, Bio-Rad, Hercules, CA, USA). The membrane was blocked for 2 h in TBS-T (tris buffered saline supplemented with 0.1 % Tween ...
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bioRxiv - Immunology 2022Quote: The purity and quality of the proteins were characterized at all stages of the purification process using 4%-20% SDS–PAGE (Bio-Rad, Catalog number 4561096) and 3%-12% Bis-Tris gel (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins (40 μg/lane) were resolved on a 4–20% Mini-PROTEAN® TGX Stain-Free™ precast polyacrylamide gel (Cat.#4568094; Bio-Rad Laboratories) and transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: Approximately 15 μg of synaptosome protein was separated on a precast gradient gel (4-15% Mini-PROTEAN® TGX™ Precast Protein Gels, 4561086, BioRad, United Kingdom) and transferred to nitrocellulose membrane (Amersham™ ProtranÒ Western Blotting Membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples containing 30 μg of total protein were separated by SDS-PAGE for 30 min at 270 V using 4–20% gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of total protein for both NT and Rab siRNA-transfected samples were resolved in 4-20% gradient SDS-PAGE (Mini-PROTEAN Gel, Cat. 4561094, Bio-Rad Laboratories, Hercules, CA) and subsequently transferred to a PVDF membrane for immunoblotting (Immun-Blot PVDF Membrane ...
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bioRxiv - Microbiology 2023Quote: ... Samples were boiled in Laemmli sample buffer before separation by SDS-PAGE on 4-20% TGX™ precast gels (Bio-Rad, Hercules, CA, USA). Gels were transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: Protein lysates (40 µg per lane) from uteri or oviducts were loaded on a 4-15% SDS-PAGE gel (Bio-Rad, Hercules, CA, USA), separated in 1X Tris-Glycine Buffer (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–20% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Developmental Biology 2023Quote: Samples were run on 10% or 4-15% TGX pre-cast gels with 10 wells with 50 μL well volume (Bio-Rad, #4561034 and #4561084). Proteins were transferred overnight onto PVDF membrane (wetted briefly with methanol ...
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bioRxiv - Biophysics 2023Quote: ... the protein concentrations were determined by image analysis of SDS-PAGE with 4-20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad, 4568096) followed by Coomassie Brilliant Blue staining using the following concentration standard ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Physiology 2023Quote: ... 5 μg of protein homogenate was separated on Criterion™ XT Bis-Tris (RyR, SERCA, Actin) or 4–15% Mini-PROTEAN® TGX™ Precast stain-free (CaMKIIδ) gels (Bio-rad) and transferred at 4°C for 16 hrs at 30 V onto 0.45 µm supported nitrocellulose membrane (Bio-rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 25ug of protein was used for Western blot run in 4-20% gradient gel (Criterion TGX Stain-free, #5678094, Bio-Rad, Hercules, California, USA). Proteins were transferred to nitrocellulose membrane in Turboblotter (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting proteins were separated by polyacrylamide gel electrophoresis (4%–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, Bio-Rad, California, USA) and then transferred to a nitrocellulose membrane (Reference ...
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bioRxiv - Genetics 2023Quote: ... The purity of viral preparations was determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis on a 4– 15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Hercules, CA, USA) in Tris-glycine- SDS buffer (Figure S3).
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bioRxiv - Bioengineering 2023Quote: ... was subjected to SDS-PAGE using a 4-20% Mini-PROTEAN TGX acrylamide gel on a Mini-PROTEAN Electrophoresis cell (Bio-Rad Laboratories, CA, USA). Electrophoretically separated proteins were transferred to Immobilon-P PVDF membrane (25V/RT/overnight) ...
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bioRxiv - Neuroscience 2024Quote: ... 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad, Hercules, CA, Criterion XT 3450125) in XT MES buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently subjected to SDS-polyacrylamide gel electrophoresis (PAGE) on a 4–15% Mini Protean TGX precast gel (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Animal Behavior and Cognition 2024Quote: Whole cell lysates containing SDS buffer were homogenized with a 27-G syringe and resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels and transferred to nitrocellulose membranes (Fisher Scientific ...