Labshake search
Citations for Bio-Rad :
4151 - 4200 of 7697 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... were detected as dark spots after a 10-min reaction with 5-bromo-4-chloro-3-idolyl phosphate and nitro blue tetrazolium using an alkaline-phosphatase-conjugate substrate (Bio-Rad, CA, USA). SFUs were counted using the AID ELISpot Reader System (Autoimmun Diagnostika) ...
-
bioRxiv - Cell Biology 2023Quote: ... 20-40 μg protein were separated by SDS-PAGE on 4-15% Mini- PROTEAN® TGX Stain-Free™ Gels (Bio-Rad®). and transferred onto PVDF membranes using Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad®) ...
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was collected and incubated at 95°C for 3 min in SDS sample buffer before separation by SDS–PAGE on 4–15% gradient Mini-PROTEAN TGX precast polyacrylamide gels and transferred to nitrocellulose membranes (Bio-Rad, Hercules CA). Membranes were next blocked with 5% milk in TBS with 0.1% Tween 20 and incubated with primary antibodies and then HRP-coupled secondary antibodies in 5% milk or bovine serum albumin in TBS with 0.1% Tween 20 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Equal quantity of the protein was resolved in a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels and transferred to an Immun-Blot PVDF Membrane (Bio-Rad) for antibody incubation ...
-
bioRxiv - Neuroscience 2023Quote: ... heated for 5 min at 95°C and loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Germany). Proteins were transferred on nitrocellulose membranes and membranes were blocked with 5% BSA in PBS-Tween ...
-
bioRxiv - Molecular Biology 2023Quote: 20-25μg of protein lysate with 4x Laemmli buffer (cat# 1610747; Bio-Rad Laboratories was separated on 4-15% Mini-PROTEAN TGX Precast Protein Gels (cat# 4561086; Bio-Rad Laboratories) and transferred to Immun-Blot PVDF Membranes for Protein Blotting (cat# 1620177 ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 16,000Xg for 30 min at 4°C then protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad #5000205). For each sample ...
-
bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
-
bioRxiv - Genetics 2023Quote: ... Bromophenol blue buffer was loaded on 1.0 mm thick Criterion XT Tris acetate (poly-acrylamide) gels with a linear resolving gel gradient of 4-12% (Biorad; Lunteren, The Netherlands). Gels were run at 75 V (0.07 A ...
-
bioRxiv - Developmental Biology 2023Quote: Proteins from worm lysates or coIP experiments were separated on 10% or 4-20% gradient Mini-PROTEAN TGX Precast Gels (Bio-Rad Laboratories, Inc.) at 300V ...
-
bioRxiv - Biochemistry 2023Quote: Final carboxysome samples and as well as the lysate were analyzed for presence of CsoSCA or sfGFP-fusion protein by SDS-page (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad)) ...
-
bioRxiv - Bioengineering 2023Quote: ... at 100V with a constant current for 60 mins at 4°C in a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) according to the manufacturer’s specifications ...
-
bioRxiv - Plant Biology 2023Quote: ... Fifty micrograms of protein for each treatment was loaded onto a (4-20)% acrylamide SDS-PAGE denaturing protein gel (Bio-Rad, Hercules, USA) and separated at 4°C at 150 constant volts ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal volumes of the purified fractions were analyzed on a polyacrylamide gel (4–20% TGX Precast Gels, Bio-Rad, Marnes-la-Coquette, France). Total actin was then determined by immunoblotting actin for each experimental condition ...
-
bioRxiv - Cell Biology 2024Quote: ... 4 µl of the diluted cDNA was added to a Master mix containing 4 µl SsoAdvanced Universal SYBR® Green Supermix (Bio-rad #1725270) and 0.5 µl of a 10 µM stock of each Forward and Reverse primer (Table 5 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Twenty micrograms of whole cell extracts were resolved on 4−12% SDS-polyacrylamide gels and transferred onto Ready gel blotting sandwiches immune-blot PVDF membranes (Biorad, Hercules, CA, USA) using a XCell II™ Blot Module (Invitrogen ...
-
bioRxiv - Molecular Biology 2024Quote: ... 25 μg protein were subjected to electrophoretic run using a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories), transferred to PVDF membranes using the Trans-Blot® Turbo™ Midi PVDF Transfer Packs (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... were pipetted into wells of a 18-well Criterion™ TGX Stain- Free™ pre-cast gel (4-15%, BioRad Cat. No. 5678084) and separated by SDS-PAGE ...
-
bioRxiv - Genomics 2023Quote: ... 20-25ug of protein lysate was loaded per well and separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gel (Bio-Rad). Proteins were transferred onto an activated PVDF membrane using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... for DpnII digested DNA or primers 3 and 4 (table S3) for NlaIII digested DNA on a thermal cycler (Bio-Rad C1000 Touch) with the block settings shown in table 1 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The centrifuged samples (10 μL sample volume) were loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA, Cat # 4561086) and run at 140 V ...
-
bioRxiv - Biophysics 2023Quote: ... Cleavage of LD650-labelled GPΔTM was verified by in-gel fluorescence imaging of uncleaved and cleaved protein on a 4-20% polyacrylamide using a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA) followed by Coomassie staining ...
-
bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
-
bioRxiv - Physiology 2024Quote: ... Thirty µg of total protein from each sample was loaded into a 4–15% gradient Criterion Tris-Glycine extended (TGX) Stain Free gel (#5678085, Bio-Rad, Gladesville, Australia), separated via electrophoresis at 200 V ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were placed on a heat block (+90°C) for 10 minutes and separated by SDS–polyacrylamide gel electrophoresis (4–20% Mini-PROTEAN TGX gels, Bio-Rad, 456-1096). Proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
-
bioRxiv - Bioengineering 2024Quote: ... were separated on a 4–10% gradient sodium dodecyl sulfate-polyacrylamide gel at 120 V for 90 min using a PowerPac Basic setup (Bio-Rad Laboratories, Inc.). Next ...
-
bioRxiv - Neuroscience 2024Quote: ... was then added and the samples were boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... boiled for 3 minutes at 95°C and directly loaded into a 10 well Mini-Protean TGX Precast Gel with a 4-15% polyacrylamide gel gradient with 50 µl volume per well (Biorad, Cat#456-1084). The gel was run in running buffer (composition below ...
-
bioRxiv - Bioengineering 2024Quote: ... and 8ug of total protein from each lysate was separated on a 4-15% SDS-polyacrylamide gel (Mini-PROTEAN® TGX™ Precast Protein Gels (BIO-RAD). After the proteins were transferred to nitrocellulose membrane (BIO-RAD) ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were boiled at 95°C for 5 min and resolved in 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed overnight at 4°C with primary antibodies ...
-
bioRxiv - Cell Biology 2024Quote: ... Twenty micrograms of protein per sample were loaded into each lane of 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were transferred to nitrocellulose membrane using semidry Trans- Blot® Turbo™ Transfer system (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
-
bioRxiv - Cancer Biology 2024Quote: ... 30 ug of protein was loaded in each lane of a 4– 15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad 4561083). After SDS-PAGE ...
-
bioRxiv - Biophysics 2019Quote: ... Gel precursor solutions were prepared with 6% v/v acrylamide and 0.2% v/v bisacrylamide (30% acrylamide/bisacrylamide stock 29:1, BioRad), 2 mM LAP photoinitiator (Sigma ...
-
bioRxiv - Genetics 2023Quote: ... followed by purification and size selection on 1% agarose gel with .6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
-
bioRxiv - Microbiology 2021Quote: ... pulse times 6-36 s at 6 V/cm on a Bio-Rad CHEF MAPPER apparatus (Bio-Rad Laboratories). Restriction profiles were analyzed using the BioNumerics version 7.10 finger-printing software.
-
bioRxiv - Cell Biology 2020Quote: ... were harvested and resuspended in 1X PBS-NaN3 (0.2%) and embedded in 2% low-melt agarose (Mb grade, BioRad). Agarose plugs were chilled at 4°C for 30 min and then ejected into Proteinase K digestion buffer (1 mg/mL proteinase K ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated in 2-mm cuvettes (600 V, 50 μF, 200 Ω) by using Gene Pulser Xcell (Biorad). Immediately after electroporation ...
-
bioRxiv - Molecular Biology 2021Quote: ... resolved by size on a 2% agarose gel and imaged on Gel Doc XR+ and ChemiDoc XRS+ systems (Biorad).
-
bioRxiv - Cell Biology 2022Quote: ... The reaction mixture (total volume 22 μl) contained 11 μl of 2× ddPCR Super Mix for Probes (BioRad, 1863024), 1.1 μl of 4 primers mixture 18 μM each ...
-
bioRxiv - Pathology 2019Quote: ... by SDS-PAGE at 100 V for 2 hours in a Mini-PROTEAN® Tetra cell apparatus (Bio-Rad). The Kaleidoscope commercial standard Precision Plus Protein Standard (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: ... Reverse Transcription cDNA synthesis reactions were performed on 0.2 μg −2 μg total RNA with iScript cDNA synthesis kit (BioRad) according to manufacturer’s instructions ...
-
bioRxiv - Pathology 2019Quote: ... The cDNA was then diluted 10x with water and 2 µl was mixed with iQ SYBR Green Supermix (BioRad) containing 100 nM of forward and reverse primers ...
-
bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed in 20 μL reactions in triplicate containing 10 μL 2 × SsoFast EvaGreen Supermix (Bio-Rad,), 1 μL of primers (3 μM F and 3 μM R) ...
-
bioRxiv - Microbiology 2021Quote: ... the plates were sealed and incubated at 37°C for 2 h in a T100 Thermal Cycler (Bio-Rad).
-
bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
-
bioRxiv - Cancer Biology 2021Quote: Protein lysates from SARS-CoV-2 infected Vero E6 cells were harvested in 2x Laemmli sample buffer (Bio-Rad) containing 2-Mercaptoethanol (Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 150 mM KCl and 2 mM MgCl2) at 37°C for 16 hours in thermal cycler (Bio-Rad, USA). The samples were separated using 10% non-denaturing polyacrylamide gel and tris/boric acid/EDTA buffer (TBE ...
-
bioRxiv - Cancer Biology 2022Quote: ... PCR products were run on 2% agarose gel and visualized in a Gel Doc EZ System (Bio-Rad, USA). GAPDH served as an endogenous control.
-
bioRxiv - Microbiology 2020Quote: ... and 30μg of total protein per sample was mixed with Laemmli buffer supplemented with 2-mercaptoethanol (10%) (Bio-rad) and boiled at 99°C for 10 minutes ...