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Citations for Bio-Rad :
4501 - 4550 of 7697 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The pellets were resuspended with 2 % SDS and loaded onto a cellulose acetate membrane assembled in a slot blot apparatus (Bio-Rad). The membrane was washed with 0.2% SDS and retained SDS-insoluble mHTT aggregates were detected with mouse anti-polyQ antibody (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral copy numbers were determined using SARS-CoV-2 RdRp gene specific primers and probe (see [28]) and droplet digital PCR (Bio-Rad Laboratories Inc. ...
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bioRxiv - Immunology 2022Quote: Serum samples were obtained from blood collected from the submandibular vein at the indicated timepoints and incubated on Kallestad HEp-2 slides (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were prepared for loading by mixing 30 μg of protein in 5xSDS sample buffer containing 15% 2-Mercaptoethanol (Bio-Rad) and heated at 90 °C for 5 minutes ...
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bioRxiv - Physiology 2022Quote: ... followed by a final melting curve analysis from 70°C to 95°C with 0.2°C increment for each step using the CFX96 Real-Time PCR System and the Precision Melt Analysis software (Bio-Rad). For HMA analysis ...
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bioRxiv - Genomics 2024Quote: ... Samples were then run on a 2% agarose gel with 1x SYBR Safe for 2 hours at 120 V before gel extraction with a Freeze ‘N Squeeze column (Bio-Rad). After extraction ...
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bioRxiv - Genomics 2024Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed with the 2× S6 SYBR Premix EsTaq plus (Scintol) on the CFX Connect Real-Time System (Bio-Rad). The data were analysed using the 2-ΔΔCt method ...
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bioRxiv - Neuroscience 2023Quote: ... and the supernatants were incubated with either 2 µg of anti-PAR antibody (clone #19, custom designed at AbD Serotec, Bio-Rad) or Human IgG F(ab)2 (Rockland ...
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bioRxiv - Genomics 2024Quote: ... This cDNA was then run on a 2% agarose gel in TAE buffer alongside a 100bp-1kb DNA ladder (Bio-Rad). After electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was filtered through a 0.4 μM filter to remove the insoluble fraction and supplemented with 30 mM Imidazole pH 7.0 immediately before loading at a flow rate of 2 mL/min onto a 5 mL Ni-NTA immobilized metal affinity chromatography (IMAC) column (Bio-Rad) equilibrated in Buffer A (50 mM Tris-Cl ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM 2-mercaptoethanol) and protein concentration measured using Bradford method (Bio-Rad Protein Assay Dye Reagent Concentrate; Bio-Rad Laboratories).
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data are expressed as OD595 of selected timepoint relative to OD595 at day 0.
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bioRxiv - Cancer Biology 2023Quote: ... and the fold change was calculated using the 2−ΔΔCT method using Bio-Rad CFX384 Touch Real-Time PCR System and software (Bio-Rad). The results were expressed either as the relative log2 FC (fold change ...
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bioRxiv - Biophysics 2023Quote: ... The subsequent mixture was gently rotated at RT for 20 min prior to the addition of 250 mg SM-2 Bio-Beads (Bio-Rad) per 5 ml of sample ...
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bioRxiv - Cell Biology 2023Quote: ... The total cDNA obtained was diluted 1:10 and 2 µl was used for qRT-PCR by using gene specific primer and SYBR green PCR mix (BIO-RAD) on BIO-RAD CFX96 real time PCR machine ...
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bioRxiv - Immunology 2023Quote: ... Labeled glycans were dissolved in HPLC grade water (FisherScientific) and excess unbound APTS removed using Agencourt CleanSEQ beads (non-antigen specific glycans) and Bio-Gel P-2 (Bio-rad) size exclusion resin (RBD-specific glycans) ...
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bioRxiv - Neuroscience 2023Quote: ... F0 fish were screened by high resolution melt (HRM) analysis using gene-specific primers (Table 2) with Precision melt supermix (Biorad 1725112) according to the manufacturer’s protocol in a BioRad CFX RT-PCR thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR products were run on a 2% agarose gel and images were taken on a Chemidoc MP imaging system (BIO-RAD). Image J was used to analyze the pixel intensity of 3R (288 bp ...
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bioRxiv - Biochemistry 2023Quote: ... for 2 h (for the ERGIC-53 ΔΗ34 mutant) The beads were transferred into a gravity flow column (Econo-column, Bio-Rad) and washed with 20 CV of buffer A supplemented with 0.02% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and transfers of proteins to .45uM nitrocellulose membranes were performed using the Protean 2 or 3 systems (Bio-Rad) and protocols provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... was used for positive signals and the chemiluminescence was detected using a Light Capture AE-6971/2 device (ATTO) or ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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bioRxiv - Cell Biology 2023Quote: ... Protein content was confirmed by mixing 2 μL of each elution step with 200 μL Bradford solution (Protein assay dye reagent (Bio-Rad) diluted 1:5 in H2O) ...
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bioRxiv - Genomics 2023Quote: ... 10ul of PCR product of each replicate for each gene was loaded in separate wells in 2% agarose gels and imaged using a ChemiDocTM MP Imaging system (BioRad, USA). All replicates for each gene were loaded on the same gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 2 ml of 10% sorbitol and 40 μl per transformation mixed with up to 5 μl of plasmids were electroporated in 2 mm cuvettes (Gene Pulser Xcell BIO-RAD). For autoactivation controls ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of RNA template was converted to cDNA using iScript™ cDNA Synthesis Kit (1708891, Bio-Rad, Hercules, CA, USA), according to the manufactures’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixtures contained 100 ng RNA template per sample diluted 2-fold with qPCR solution (iTaq Universal SYBR Green One-Step Kit, Bio-Rad) containing the primers D2S and D2C ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Specific antigens were detected after incubating 2 h at room temperature goat anti-rabbit IgG horseradish peroxidase conjugate (BioRad Laboratories, Inc.). Immunoreactivity was visualized by electro-chemiluminescence in a Chemi-Doc MP Imaging System (Bio Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed from 2 to 5 µg of RNA with iScript Adv cDNA synthesis kit (Bio-Rad #1725038). The cDNA was diluted 5 times and 1 μL of this was used for each reaction in real-time PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Cancer Biology 2023Quote: ... before solubilisation in 2% SDS (Severn Biotech, Worcs, UK) and subsequent OD595 measurements were obtained using an iMark microplate reader (Bio-Rad). Data were normalised by dividing raw OCR/ECAR measurement by OD595 for each well of the culture plate ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Reaction products were visualized on a 2 % agarose gel with 0.05% (v/v) Ethidium Bromide and visualized with a Gel Doc XR+ imager (Bio-Rad Laboratories). PCR amplicons were column purified with the Monarch® PCR & DNA Cleanup Kit and eluted in 25 µL of Monarch® DNA Elution Buffer.
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bioRxiv - Biophysics 2024Quote: ... then diluted below the CMC of C12E8 with reconstitution buffer and added to 0.5 g methanol-activated Biobeads SM-2 (Bio-Rad; 1523920). The mixture was rotated end over end for 18 hours and then subject to room temperature size exclusion chromatography on a Superose 6 10/300 GL column ...
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bioRxiv - Genetics 2024Quote: ... Membranes were were in contact with the luminol-H2O2 solution for 2 minutes and then exposed for imaging using the Chemidoc imaging system (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... 10 µg Capped mRNA transcripts were electroporated into BHK-21 cells in a 0.2 cm cuvette (25 μF, 1.5 kV, and 200 Ω) with the help of Gene Pulser Xcell Electroporation system (Bio-Rad) and resuspended in antibiotic-free DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: Proteins (20 µg per well) were separated in 4–15% Criterion™ TGX™ Precast Midi Protein Gels with 26 wells (Bio-Rad, Hercules, CA, USA) at a constant voltage of 120 V ...
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bioRxiv - Pathology 2019Quote: ... Samples were mixed with SDS-loading buffer without heating and electrophoretically separated on a 4–20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-rad, cat. number 5671094) and transferred to nitrocellulose membrane ...
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bioRxiv - Microbiology 2020Quote: ... 8.4 μL of PCR Probe water and 10 μL of the 2x commercial reaction mixture (SYBR® Green Master Mix, Bio-Rad. A LightCycler96® (Roche) device was used and PCR conditions applied were ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min before loading into a 4 – 20% Criterion TGX precast Midi protein gel (Bio-Rad, Hercules, CA, USA; Cat# 5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2019Quote: ... were separated on 10% or 4-20% gradient SDS–PAGE gels and wet-transferred to PVDF using the mini-PROTEAN® TGX™ system (Bio-Rad, Hercules, CA, USA). Membranes were pre-blocked with 5% low-fat dry milk in TBS-T and incubated with a rabbit polyclonal anti-KHSRP antibody (Sigma-Aldrich Cat# HPA034739 ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were incubated 5 min at 95 °C in this solution and 20 µL of supernatant was analyzed on gradient gels (BioRad 4-12% TGX pre-cast gels). The modified proteins were detected with an infrared imager (Odyssey ...
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bioRxiv - Biochemistry 2021Quote: The proteins extracted from Ni-NTA resin were subjected to SDS-PAGE on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA, USA) using the standard procedure ...
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bioRxiv - Microbiology 2022Quote: ... The lysates were adjusted to 5 µg of total soluble proteins in native sample buffer and then loaded onto a 4-20% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad, Cat. No. 4561094). Protein electrophoreses were performed in TG buffer (60 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... The purification is assessed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; precast 4 to 20 % gradient from Bio-Rad stained with Coomassie Brilliant Blue), but proteins typically elute in the 100 mM fraction ...