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Citations for Bio-Rad :
4401 - 4450 of 7697 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Equal amounts of protein were loaded onto 12% (v/v) resolving SDS-PAGE gels with 4% (v/v) polyacrylamide stacking gels using Precision Plus Protein™ dual colour standards (Bio-Rad, Hercules, CA, USA). The gels were run in a Mini-Protean® Tetra Cell system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Purified proteins were eluted in SDS-PAGE sample loading buffer and separated in 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad# 4561094, 4561096) either for western blot (WB ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates were prepared using RIPA buffer (see lysis and click chemistry methods below) and run on SDS Tris-Glycine gels (Precast 4 - 20% Bio-Rad, or hand-casted 20%). Gels were transferred to low-background PVDF membranes using the BioRad TransBlot Turbo system and blocked with 5% milk powder in TBS + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2020Quote: Twenty-fold concentrated viral stocks or purified rgp120 were separated by SDS-PAGE under reducing conditions using a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc. Hercules, CA), and then transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... and were heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (Bio-Rad, 4–15% Tris/Glycine) and then transferred onto 0.2 µm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified at 13,200 rpm for 15 min at 4°C prior to quantification by Lowry assay (Bio-Rad cat # 5000113 and cat # 5000114). Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Sample preparations were run on a 15% separating gel with a 4% stacking gel under reducing conditions using Tris-glycine running buffer (mini-PROTEAN system, Bio-Rad Laboratories, Hercules, CA, USA). Silver staining using sodium thiosulphate sensitisation and silver nitrate was performed according to established methods (22).
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bioRxiv - Physiology 2020Quote: ... Amplification reactions and disassociation curves of 4-fold dilution series of cDNA were carried out on a CFX384 machine (Bio-Rad, Bio-Rad, Mississauga, ON), for determination of primer efficiencies ...
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bioRxiv - Physiology 2020Quote: ... and an equal amount of total protein (30 μg) per sample was subjected to standard SDS-PAGE using Mini-PROTEAN® TGX™ 4-20% 12-wells gels (Bio-Rad, Hercules, CA). Gels were electroblotted onto PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE (4-15% acrylamide, 150V) and transferred to PVDF membranes using the Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories, CA). Membranes were incubated for 1 hr in 5% milk (in TBS-T ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed using the primer pairs listed in Table 4 and iTaq Universal SYBR Green Master Mix on a CFX384™ Real-Time PCR detection system (Bio-Rad Laboratories, Hercules, CA). Fold changes in gene expression were calculated by the ΔΔCt method using the weighted average of Ct values for the housekeeping genes Pol2ra ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2022Quote: For each lane, ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4-20% gradient gels (Bio-Rad #4561095; Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 20 μg of proteins were analyzed in a polyacrylamide gel (4–20% Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Marnes-la-Coquette, France) and separated by electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels, Bio-Rad Laboratories, CA) and transferred onto PVDF membranes (Amersham Hybond ...
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bioRxiv - Immunology 2024Quote: ... were resolved by SDS-PAGE using 4-20% Mini-PROTEAN® TGX Stain-Free Precast™ Gels gels and transferred onto Trans-Blot® Turbo™ membranes (Bio-Rad). Membranes were blocked with 5% non-fat milk in TBS-T for 1 hour at room temperature and probed with primary antibodies against (Influenza A virus NS1 ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µL of media was reduced and denatured prior to gel electrophoresis on a 4-15% tris-glycine gel (#4561084, Bio-Rad Laboratories, Hercules, CA, USA) for 2 hours @ 175 mV ...
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bioRxiv - Biochemistry 2024Quote: ... 20 to 30 µg of total protein was separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084) followed by an overnight transfer and western blot analysis.
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
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bioRxiv - Cell Biology 2024Quote: ... After gel polymerization was performed pre-electrophoresis of empty gel in 5% acetic acid (running buffer) for 1-1.5h/150 V in reverse polarity of electrophoretic BioRad system (Bio-rad, USA). The running buffer was discarded ...
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bioRxiv - Microbiology 2020Quote: Surface Plasmon Resonance (SPR) binding experiments were carried out on a ProteOn XPR36 6×6 channels protein interaction array system (Bio-Rad). Proteins BRD4-BD1 an BRD4-BD2 were diluted to a final concentration of 50μg/ml in HEPES buffer 25 mM pH 7.0 and immobilized to a GLM chip using amine coupling ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of ion exchange-purified phage was added to 4 µl of cells and after 5 min of incubation mixed 1:2 (v/v) with 6 nm BSA-gold tracers (Aurion) which were transferred to RCV by Micro Bio-Spin® 6 columns (Biorad). Four µl of the mixture were applied onto glow-discharged Quantifoil® R2/1 200 mesh copper grids ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... The libraries were quantified and checked for amplifiable adapters using the Library Quantification DNA standards 1-6 (Kappa Biosystems, Wilmington, USA) with the SsoFast EvaGreen qPCR supermix (Bio-Rad) using 10 μL EvaGreen master mix ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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bioRxiv - Cell Biology 2020Quote: Cells cultured in each well of a 6-well plate at 80% confluency were washed three times with PBS and harvested/resuspended in 150–200 μl of 2× Laemmli buffer (Bio-Rad). The cell lysate was boiled for 10 min and loaded onto an SDS gel ...
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bioRxiv - Cell Biology 2020Quote: ... Relative mRNA levels of the indicated genes were calculated by the 2-DDCT method (Bulletin 5279, Real-Time PCR Applications Guide, Bio-Rad), using GAPDH expression as endogenous control ...
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bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 2-3 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then diluted 1:10 and 2 µL of diluted cDNA were used for RT-qPCR along with 5 µL of iTaq universal SYBR mix (Bio-Rad), 1.5 µL of primers (1.5 µM mix of forward and reverse ...
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bioRxiv - Molecular Biology 2021Quote: ... was used with the primers listed in Supplementary Information Table 2 and samples were run on CFX96 Real-Time PCR Detection System (Bio-rad).
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bioRxiv - Immunology 2022Quote: ... and by linear regression in log10 copies/ml with a standard curve realized from a SARS-CoV-2 positive nasopharyngeal sample quantified by Droplet-Digital PCR (Bio-Rad). IC50s were analyzed by nonlinear regression using a four-parameter dosage-response variable slope model with the GraphPad Prism 8.0.2 software (GraphPad Software ...
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bioRxiv - Immunology 2021Quote: ... Lysates were finally adjusted to a final concentration of 2 µg/µl with 4X Laemmli buffer (Bio-Rad, Hercules, CA, USA) containing 10% β-Mercaptoethanol.
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bioRxiv - Biochemistry 2019Quote: ... MenD mutants were generated using the pYUB28b-menD construct and oligonucleotide primers (Supplementary Table 2, Integrated DNA Technologies) with iProofTM high-fidelity DNA polymerase (Bio-Rad). The PCR products were then treated with DpnI and ligated using T4 DNA ligase (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... was determined for different genes (primers are listed in Table 2) by quantitative PCR (qPCR) using iTaq SYBR Green (Bio-Rad) with 400 pg of cDNA and 0.25 μM each primer in 10 μL reactions ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were then washed three times with DI water and stained with 2 ml hematoxylin (Bio-Rad Laboratories, Hercules, CA, USA) for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We then performed quantitative PCR (qPCR) using 2 ng of cDNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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bioRxiv - Microbiology 2019Quote: T1L virions (2×1012 particles/ml) were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of the reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and indicated primers (2 μL) were added in the PCR reactions (ddPCR Supermix for Probes (No dUTP) mix from Bio-Rad) in a total of 25 μL ...