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Citations for Bio-Rad :
4101 - 4150 of 4802 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates (10-50 μg) were subjected to SDS-polyacrylamide gel electrophoresis on a 4-20% precast gradient gels (BioRad, 5678094) and proteins were transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: The source lysates of Brl1 APs 90 min post labeling were adjusted to 50 µL with a protein concentration of 4 µg/µL with lysis buffer as determined by the Bradford method (Bio-Rad). Samples were diluted with 200 µL guanidine chloride (7 M in 100 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: ... 25µg of protein was loaded per lane into an SDS-PAGE gel (Bio-Rad Mini-Protean pre-cast gels 4-20%) and gels were run at 160V for approximately 90 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein samples (cell lysates and DRG fractionized membrane and cytosol) were size separated using 10% or 4-20% SDS-PAGE gels (Bio-Rad), transferred to Immun-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... yield and level of purity were determined by SDS-PAGE analysis using 4–20 % or 12 % Mini-Protean TGX precast gels from Bio-Rad. Gels were stained with QC Colloidal stain (3 ng sensitivity ...
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bioRxiv - Microbiology 2020Quote: ... Cellular protein lysates were resolved on Criterion or 4%–20% Bio-Rad precast SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). Membranes were saturated and proteins were blotted with antibodies in 5% non-fat dry milk ...
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bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... heated for 10 min at 70C then run out on a 4-12% Novex NuPAGE gel in 1X NuPAGE MOPS buffer with a protein ladder(Bio-Rad). The gel was transferred to a 0.2um PVDF membrane for 2 hours at 200mA at 4C ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl of the protein extracts were loaded on a 4-12% gradient 26 well Criterion XT Bis-Tris Protein gel (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were centrifuged at 20000 g for 20 min at 4 °C and then filtered by gravity using Poly-Prep chromatography columns (731-1550, Bio-Rad). Supernatants were filtered by gravity through Poly-Prep chromatography columns (731-1550 ...
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bioRxiv - Molecular Biology 2020Quote: Samples were separated using 10-15% SDS-PAGE gels or 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad, 5678095). Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Equal amounts of protein from (30 μg, mouse cortex was used) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2020Quote: ... 1μg of pKSB vector containing aimed mutation and the bacterial liquid were mixed at 4℃ and electroporated by Micropulser (Bio-Rad) with Ec2 (2.5kV ...
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bioRxiv - Neuroscience 2020Quote: ... After rinses with PBS-0.1% Tween 20 (PBST), sections were incubated overnight at 4°C with rabbit anti-Iba1 (Wako, 019-19741) and anti-BrdU (Biorad, MCA2060). After washes with PBST ...
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bioRxiv - Neuroscience 2021Quote: ... samples were separated by electrophoresis on 4-20% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad Laboratories, NSW, Australia) in running buffer (0.1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: Enriched CCV fractions were resolved via 1D SDS-PAGE on a 4-15% Tris-HCl gradient gel (BioRad cat# 161-1158) at a constant 200V for ∼90 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of each sample were mix with sample buffer loaded and separated using Mini-PROTEAN TGX 4-15% polyacrylamide gels (BioRad: 4561083) and Tris/Glycine/SDS buffer (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini- PROTEAN® TGX Stain-Free™ Gels (BioRad; Cat#17000435).
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bioRxiv - Biochemistry 2021Quote: ... The cell extract was clarified by filtering through 0.4 µm and 0.22 µm filters prior to loading onto ~4 mL of Ni-NTA resin (Profinity™ IMAC resin, Bio-Rad). To allow the His-tag protein to bind the resin it was incubated overnight at 4°C on a rolling shaker ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 µL per reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 µl reactions ...
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bioRxiv - Neuroscience 2021Quote: Equal volumes of ISF were boiled at 98°C for 5 min in 50 mM of DTT and 1X LDS and separated by electrophoresis on 4-12% Bis-Tris Precast Gels (BioRad #3450124). Proteins were transferred onto 0.2 μm PVDF membranes (Biorad #1704157 ...
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bioRxiv - Neuroscience 2022Quote: ... 10µg of protein per sample was loaded into 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and run at 105V for 90 minutes ...
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bioRxiv - Physiology 2022Quote: ... we ran similar amounts of protein per lane (~10-20 μg, depending on target) on a 4-20% Criterion TGX stain-free gel (Bio-Rad) at 200 V for 50 min with the cassette surrounded by ice ...
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bioRxiv - Immunology 2022Quote: ... boiled for 10 min and subjected to protein separation by SDS-PAGE in 4 – 20% Mini-PROTEAN TGX precast gels (Bio-Rad) before transferred to nitrocellulose membranes (Wako) ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 10% neutral buffered formalin at 4°C overnight and then mCherry-positive plaques were visualized using a ChemiDoc MP imaging system (Bio-Rad). Afterwards ...
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bioRxiv - Biochemistry 2022Quote: ... Both input and IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed by Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... Input and MOV10/IgG IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed through Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
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bioRxiv - Developmental Biology 2022Quote: ... The suspended eggs and siRNA (5 μg/μl stock solution, in MilliQ) were transferred to a 4 mm cuvette (Bio-Rad) with a final volume of 200 μl and were gently mixed ...
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bioRxiv - Biochemistry 2019Quote: ... The input and IP samples were run on 4-12 % SDS-PAGE and transferred to PVDF membranes (Bio-Rad, 162-0177) using Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell (BioRad ...
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bioRxiv - Genomics 2019Quote: ... PCR products were separated on a 4% agarose gel and the length of the products was analysed using the Image Lab version 5.0 software (Bio-Rad Laboratories). PCR products were purified with QIAquick Gel Extraction kit (QIAgen ...
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bioRxiv - Neuroscience 2019Quote: ... Final purification was performed by size-exclusion chromatography and the purity of the proteins was assessed by 4%-15% SDS-PAGE gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... About 10 µg of the exosomal protein was separated on gradient (4-12%) SDS-PAGE gel and followed by transfer to nitrocellulose membrane (Bio-Rad). After blocking with 5% skim milk powder in Tris-buffered saline (TBS)-Tween for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts (15 µg) of protein lysates were heat denatured and separated on a 4–10% Mini-Protean Precast Gel (Bio-Rad), followed by transfer on a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 uL/reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 uL reactions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The boiled samples were centrifuged at 14,000 rpm for 10 minutes and 20 μg of total protein per sample was then loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA) and ran at 140 V ...
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bioRxiv - Cell Biology 2019Quote: ... 3.5% β-mercaptoethanol) and samples were analysed by SDS-PAGE using 4-20% gradient Mini Protean TGX Precast Gel (Bio-Rad). Membrane was visualized for total protein with Ponceau S (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... The efficiency of labeling the oligonucleotides to α-actinin was estimated by a gel-shift assay (4%-15% gradient gel, Biorad) and determined as >95%.
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bioRxiv - Pathology 2020Quote: ... The correct size of the purified RBD-scFv protein (52.4 kDa → ∼57 kDa with glycosylation) was confirmed by protein gel electrophoresis using Mini-PROTEAN TGX Precast Gels 4-20% (Bio-RAD).
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bioRxiv - Biochemistry 2019Quote: ... 1.2 μL aliquots at various time points were quenched in 2X SDS-PAGE loading buffer (5 μL) and electrophoresed on 4 – 20 % TGX SDS-PAGE gels (Bio-Rad). Gels were imaged on a Typhoon variable mode scanner (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifugation at 14000 rpm for 8min at 4° C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). The proteins were run on 7%-15% SDS-PAGE gels (50μg per lane ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad). Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 to 25 μg for experiments that were imaged digitally) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run either at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Pathology 2020Quote: ... 5 microliters of urine were run on 4-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie Blue Stain Solution (Bio-Rad) to detect proteinuria ...
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bioRxiv - Biochemistry 2020Quote: ... in 1X TBE buffer at 220V for ∼13 min at 4°C and were imaged with a Gel Doc XR+ (Bio-RAD) and Quantity One 4.6.9 software (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: All protein isolation steps were performed at 4 °C or on ice and protein concentrations were assessed with the Bradford assay (Bio-Rad). Protein from total pancreas lysates was isolated from a small fragment of mouse pancreas and minced in lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The second-dimension gel electrophoresis was performed in 1× TBE buffer containing 0.3 μg/ml of ethidium bromide at 6.0 V/cm for 5 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was transferred to Hybond-XL (GE Healthcare).