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Citations for Bio-Rad :
4351 - 4400 of 4802 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
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bioRxiv - Biochemistry 2024Quote: ... iSAT reactions started after addition of plasmid encoding rrnB under T7 promoter at 4 nM and the reaction was immediately incubated on pre-warmed CFX Connect Real-time System (Bio-Rad) at 37 °C for 90 mins in 96 well plates (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... Each well was then blocked with 150 µL of blocking buffer consisting of 1×PBS + 4% (w/v) nonfat milk (Bio-Rad). The plates were blocked for 1 h at room temperature and then washed five times with PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... SDS-PAGE of protein extracts was performed with a BioRad Mini-Protean gel system using Mini-PROTEAN TGX Precast 4-15% polyacrylamide gels (BioRad 4561083) and blotted to Biorad Immun-Blot LF PVDF membrane (BioRad 1620174) ...
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bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... Oligomer formation was induced by incubating the peptide solution for 24 h at 4 °C and confirmed by polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining.
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then washed 4 times with PBST and then incubated in the dark for 5 min with Clarity Western ECL substrate (1705060, Bio-Rad), and imaged using the Amersham Imager 600 (GE Industries ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μL of whole tissue lysate preps) were applied to 4−15% SDS-polyacrylamide gels (Bio-Rad; Hercules, CA, USA) and subjected to electrophoresis at 180 volts for 50 minutes in a preformulated 1× SDS-PAGE buffer (VWR) ...
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bioRxiv - Immunology 2024Quote: ... 10 µL of each protein sample was then loaded and run on precast protein gels (4-20% Mini-PROTEAN® TGXTX, BioRad) before staining with Coomassie blue dye ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.4 μM final concentrations of the gene-specific PCR primers on a CFX Connect Real-Time System thermal cycler (Biorad, Hercules, California). The qPCR conditions were 95°C for 3’ ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... The monolayer was fixed with 4% paraformaldehyde and permeabilized with Triton-X100 and stained with mouse anti-NP IgG1 (clone AA5H, Bio-Rad Antibodies) followed by goat anti-mouse HRP (DAKO ...
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bioRxiv - Genetics 2021Quote: ... Forty micrograms of total protein from hippocampal nuclear lysates was boiled for 5-minutes to dissociate complexes before separation in a 4-20% gradient gel (Bio-Rad 4561096) for 1-hour at 150V in 1x Tris/Glycine/SDS (Bio-Rad 1610732) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Cell Biology 2022Quote: Protein homogenates (5 or 15 μg/lane) were size fractionated on 4–15% or 15% Criterion TGX gels (Biorad Laboratories, Oslo, Norway) and transferred to 0.45 μM PVDF-membranes (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... for the experiments done on 12-well plate) for each sample on SDS-PAGE using 4–20% Mini-PROTEAN® TGX gels (Bio-Rad), and immunoblotted as described (36) ...
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bioRxiv - Cell Biology 2019Quote: ... and densitometric analysis was performed using the Gel Doc 2000 Gel Documentation System and Quantity One version 4 software (BioRad, CA, USA). The β-tubulin signal was used to normalize protein levels.
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bioRxiv - Physiology 2020Quote: ... tissue biopsies were transferred into the assay medium for a 4h incubation (pre-incubation medium supplemented with 4 mg/ml glucose and 4.3% Alamar Blue solution (Bio-rad, Hercules, CA)) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and electroporated in a 4-mm cuvette using an exponential pulse at 250 V and 950 mF utilizing a Gene Pulser electroporation device (Bio-Rad Laboratories). We used a plasmid encoding a CMV-mCherry-Cas9 expression cassette and a human SAMHD1 gene specific gRNA driven by the U6 promoter ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured proteins (20-40 µg) were electrophoresed in 9% SDS-PAGE gels or MiniPROTEAN® TGX™ 4-15% Precast gels (BIORAD), transferred onto a nitrocellulose membrane and probed with specific antibodies ...
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bioRxiv - Zoology 2019Quote: An equal amount of protein from each one of three liver or spleen samples (each tissue was harvested from a different bird) was loaded on a Criterion XT 4-12 % gradient polyacrylamide gel (Bio-Rad, USA) and proteins were separated by SDS-PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... The blot was washed 4 times with TBS-T and subsequently incubated with a goat anti-mouse-HRP antibody (Bio-Rad #1706516) for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed with 100 μL Laemmli buffer 2x and 20 μL of each sample (including -CL) was loaded in Criterion™ TGX Stain-Free™ Gels (4-20%, BioRad). SDS-PAGE and Western blots were used to reveal the proteins HuR ...
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bioRxiv - Cancer Biology 2019Quote: ... 40µg of protein from each sample was loaded onto the 4– 15% Mini-PROTEAN® TGX™ Gel (BioRad, Catalog number: 4561083) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Physiology 2019Quote: ... Diluted proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient Criterion TGX Precast Midi Protein gel (Bio-Rad, CA), and subsequently transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... The samples collected before and after UV-crosslinking were subsequently analysed by SDS-PAGE in 4-20% gradient gels (Bio-Rad TGX). Gels were subsequently transferred onto a PVDF membrane and FLAG epitopes were detected by Sigma M2 antibodies followed by ECL imaging.
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Equal volumes of protein were loaded onto pre-cast BioRad Mini-Protean TGX precast Gel 4-20% (15-wells BioRad cat# 4568096). The gels were run according to the manufacturer’s guidelines (110Volts for 3 minutes ...
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bioRxiv - Immunology 2021Quote: ... The cells were centrifuged at 300G for 7 minutes at 4°C and the cell pellet was resuspended then counted with the BioRad cell counter (Bio-Rad, TC20).
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Electrophoretic analyses of viral proteins and equine formulations were performed in 4-20% SDS-PAGE gel Mini-PROTEAN TGX (BioRad; California, USA) and 7.5% SDS- polyacrylamide gels (SDS-PAGE) ...
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bioRxiv - Bioengineering 2020Quote: ... 4-20% Mini-PROTEAN TGX Precast Protein Gels and Precision Plus Protein Dual Color Standards were purchased from Bio-Rad (Hercules, CA). Maleimide PEG Hydroxyl (MW=5,000 g/mol ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... Samples were diluted four-fold with water before being loaded onto a 4-20% tris-glycine polyacrylamide gel (BioRad, Hercules, CA, USA), approximately ∼10µg of protein (i.e. ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of proteins were separated by denaturing electrophoresis on a 4– 15% Criterion™ TGX™ Precast Midi Protein Gel (Biorad) and then electrotransferred to nitrocellulose membranes using the Trans-blot Turbo Transfer System (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of the cleared lysate were separated by SDS-PAGE using 4%-15% Mini-PROTEAN TGX Precast Protein gels (Bio-Rad, #4561085) and blotted onto a Amersham Protran Premium Western blotting nitrocellulose membrane (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... To reduce non-specific binding single cell suspensions were incubated for 5 min at 4°C with PBS/10% AB-serum (Bio-Rad, Germany), subsequently stained with fluorescence-labeled or biotinylated antibodies for 15 min at 4°C and washed once with PBS/2% FCS/0.01% NaN3 ...
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bioRxiv - Cell Biology 2019Quote: ... The protein extract was first separated by loading equal amounts of protein on 4-20 % Mini-PROTEAN® TGX™ Gel SDS–PAGE gradient gels (456-1096, Biorad) and then transferred to the nitrocellulose membrane with Trans-Blot Turbo Transfer Pack (170-4159 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples prepared in 4X Laemmli buffer were run on a 4-20% Mini-protean TGX precast gel (Bio-Rad Laboratories, Hercules, CA) and transferred to nitrocellulose using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... 20μg of protein were loaded into each lane of a Criterion™ XT Bis-Tris Precast Gel 4-12% (Bio-Rad, UK) for SDS-Page separation ...
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bioRxiv - Plant Biology 2020Quote: ... The mixture was kept for 5 min at 95°C and 5 min on ice before loading on a TGX 4-20% Strainfree (Bio-Rad US) gel immersed in TGS 1X buffer ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad) and visualized with GelCode Blue stain (ThermoFisher).
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bioRxiv - Neuroscience 2019Quote: Samples were then prepared for western blotting in Laemmli buffer heated at 60C for 10mins and 20µg were run on a 4-20% Acrylamide gel (Biorad.cat no 456-1093). Wet western blotting was carried out using 0.2µm Nitrocellulose membranes (GE Healthcare Amersham ...
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bioRxiv - Molecular Biology 2020Quote: ... A total of 25 μg of protein was loaded onto a 4–20% Mini-PROTEAN® TGX™ Precast Gel (Bio-Rad) and transferred onto a PVDF membrane ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples (n = 80 embryos / lane) were run on a precast SDS-PAGE gel (4-20% MP TGX Stain-Free Gel, Bio-Rad 4568093), with a Precision Plus Protein WesternC Stds ladder (Bio-Rad 1610376) ...
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bioRxiv - Neuroscience 2021Quote: ... samples were transferred directly to ice-cold A4P0 solution consisting of: 4% acrylamide monomer (40% acrylamide solution, cat. 161-0140, Bio-Rad, UK), 0.25% VA-044 (thermoinitiator ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng total RNA was reverse-transcribed to cDNA in a 20 μl reaction with 4 μl iScript reverse transcription supermix (Bio-Rad #1708841) at 25 °C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... a part of each sample was run on a 4-15% Criterion TGX Stain-Free Protein Gel (26 wells, Bio-Rad, #5678085) in 1x SDS running buffer at 200 V for 32 min ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were loaded on 10% or 4-15% sodium dodecyl sulfate polyacrylamide gels (Fast Cast TGX Stain-Free or 4-15% Mini-Protean TGX Stain-Free gels, BioRad, Hercules, CA). Blotting was done on PVDF membranes using a BioRad Transblot system ...