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Citations for Bio-Rad :
4001 - 4050 of 4802 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Ten to twenty micrograms of denatured protein was loaded onto a 4-20% TGX stain-free polyacrylamide gel (Bio-Rad, USA) or NuPAGE 3-8% Tris acetate gel and subsequently transferred onto pre-cut nitrocellulose membranes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... SDS PAGE was performed using 7.5% or 4-20% Mini-PROTEAN® TGX™ precast protein gels (BIO-RAD, 4561023/4561094) run in running buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... 15µg of each sample was loaded per lane of a MIDI 4-15% Criterion Precast Midi SDS page gel (BioRad #5671085) and electrophoresis was performed at 120mV for 75 minutes ...
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bioRxiv - Developmental Biology 2020Quote: Equal amounts of protein were resolved on either a 15% or a 4-20% Tris-glycine gradient SDS-PAGE gel and transferred to a polyvinylidene difluoride Immobilon-FL membrane (PVDF; Bio-rad). Membranes were blocked for 30 min at room temperature with 5% BSA in TBST (50 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-25 μg of proteins were loaded onto 4-12% Mini-PROTEAN Tris-Tricine Precast Gels or Criterion Tris-Tricine Precast Gels (Bio-Rad), and electro-blotted on nitrocellulose membranes (GE Healthcare Life Science) ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was then added and the solution was mixed at 4°C for 10 minutes before addition of 100 mg of Biobeads SM2 (Bio-Rad). Biobeads were washed into methanol ...
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bioRxiv - Molecular Biology 2020Quote: ... aliquots of the supernatant and resuspended pellet fractions were separated by SDS-PAGE using 4-15% gradient TGX gels (Bio-Rad) and analyzed by densitometry using Image Lab software (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: 10-40 µg of denatured protein extracts were separated by SDS-PAGE using 10% acrylamide gels or 4-15% Mini-Protean precast gels (Bio-Rad) for 2 h at 120 V and transferred onto methanol-activated PVDF membranes at 350 mA for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: Samples in 1x Laemmli loading buffer (generally equivalent to 1 µL replication reaction or chromatin recovered from 8 µL plasmid pull-down) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to PVDF membranes (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the equivalent of 50 μg protein input (elution) were analyzed on a 4%-12% gradient XT precast Criterion gel using XT-MOPS buffer (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins associated with half of the Dynabeads were eluted with 22 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) by boiling at 95 °C for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... The α-FN-EDA Fab was verified as >90% pure by SDS-PAGE (performed on 4-20% gradient gels (Bio-Rad)).
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... were separated by SDS PAGE in 4-12% Bolt Bis-Tris Plus gels (ThermoFischer Scientific) and transferred to 0.2 µm Immun-Blot® PVDF membrane (BIO-RAD). After blocking with 5% (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... The denatured samples were resolved on a 4-20% Mini-PROTEAN TGX Stain-Free precast protein gel (Bio-Rad, cat# 4568094) and transferred to a nitrocellulose membrane (0.45 μm ...
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bioRxiv - Neuroscience 2019Quote: ... Protein samples were electrophoresed on NovexTM 4-20% Tris-Glycine gels (Thermo-Fisher) and transferred onto nitrocellulose membrane using the CriterionTM Blotter transfer system (Bio-Rad). Membranes were blocked in 5% milk in .1% Tween/Tris-buffered saline (TBST ...
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bioRxiv - Genetics 2019Quote: ... and 1x phosphatase inhibitors and run as previously reported (Blauwendraat et al. 2018a) on 4-20% Criterion TGX gels (Bio-Rad) in Tris-glycine running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... paraffin-embedded sections were incubated at 4°C overnight with antibodies for F4/80 (MCA497G, Bio-Rad Laboratories, Hercules, CA, USA), HILPDA (sc-137518 HIG2 Antibody (C-14) ...
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bioRxiv - Pathology 2019Quote: ... 20% methanol and MilliQ water) overnight at 4°C in a wet transfer apparatus at 70 mA constant voltage (Bio-Rad). Detection of the chemiluminescence immunochemical reaction was performed on a ChemiDoc ™ XRS + Molecular Imager® (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins and/or immunocomplexes were separated by electrophoresis on a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (Bio-Rad). Proteins were transferred to a nitrocellulose membrane and probed with anti-HA-HRP (3F10 ...
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bioRxiv - Microbiology 2019Quote: ... Alternatively broken cyst walls either before or after digestion with trypsin were reconstituted in 1× reducing SDS/PAGE loading buffer and run on a 4–20% precast polyacrylamide TGX gel (Bio-Rad). Bands stained by colloidal Coomassie blue were excised and washed with 50 mM NH4HCO3/acetonitrile (ACN) ...
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bioRxiv - Biophysics 2019Quote: ... Temperature was increased at a rate of 1°C per minute from 4°C to 100°C and fluorescence was measured every minute with a qPCR thermocycler in FRET mode (Bio-Rad). Aggregation temperature was determined by locating the global minimum of the second derivative of the raw data ...
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bioRxiv - Microbiology 2019Quote: ... in a 4 mm cuvette and then electroporated at 960 μF and 250 V with a Gene Pulser Xcell system (Bio-Rad). Electroporated cells were plated into 10 cm plates at 2 × 105 ...
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bioRxiv - Physiology 2019Quote: ... protein samples (20 µg of total protein) were separated at 300 V for 30 min using 4-20% gels (Criterion TGX, Bio-Rad), followed by wet transfer to PVDF membranes (0.2 µm Immun-Blot ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of protein per sample (5 or 10ug) was loaded into a 4-20% gradient precast mini-PROTEAN TGX gel (Bio-Rad) and proteins were separated with 200V in 1x running buffer (24.76 mM Tris base ...
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bioRxiv - Physiology 2020Quote: ... equal amounts (30 μg) of protein were resolved on 4–20% TGX stain-free gels (Bio-Rad Laboratories GmbH, Munich, Germany) and transferred onto PVDF membranes ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% CriterionTM TGXTM precast Midi protein gel (Bio-Rad) for SDS-PAGE followed by wet transfer to nitrocellulose membrane ...
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bioRxiv - Developmental Biology 2020Quote: ... excess antibody subsequently removed by a further 4 washes in TBSTw for 5 minutes before detection with ECL Western blotting reagent (Bio-Rad) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093, Bio-Rad) and transferred to PVDF membrane (1620177 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Protein concentration was quantified using a BCA assay and loaded at 25- 50ug in 1x Lamelli Buffer with B-mercaptoethanol onto a 4-15% TGX protean gel (Bio-Rad). In some experiments to achieve greater separation to detect the N-truncation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: 40-60μg of protein was electrophoresed through 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hertfordshire, UK) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Genetics 2020Quote: ... Fractions of 200 μl were collected and analyzed in a precast 4-20% gradient SDS-PAGE gel and visualized using ImageLab™ (BioRad).
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bioRxiv - Genetics 2020Quote: ... Fractions of 250 μl were collected and analyzed on a precast 4-20% gradient SDS-PAGE gel and visualized using ImageLab™ (BioRad). A size calibration curve was previously prepared using thyroglobulin (670 kDa) ...
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bioRxiv - Cancer Biology 2020Quote: ... 20µg total protein in βmercaptoethanol-containing loading buffer was added per well onto 4-12% SDS-PAGE gels (NuPAGE) and transferred to PVDF (Bio-Rad) or nitrocellulose (Amersham ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of total protein was run on a 4–15% SDS-PAGE gel (Mini-PROTEAN TGX Precast Protein Gel; Bio-Rad). Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... of each sample was loaded on an 8-16% (for TSPO, 3b-HSD, and StAR) or 4-20% (for mERα, caveolin-1, and PKA) SDS precast gels (Bio-Rad) and separated by electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... The purified proteins were separated by SDS/4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA), then transferred to an Immobilon-FL PVDF membrane (Merck Millipore Ltd. ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated using SDS-polyacrylamide gel electrophoresis (SDS-PAGE-4-12%) and transblotted onto nitrocellulose membranes (Bio-rad, California, USA). The membranes were blocked with 5% dry milk (overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were heat denatured and equal concentrations of protein were electrophoresed on 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad) and transferred onto 0.2 µm PVDF membrane in Tris-Glycine transfer buffer containing 20 % methanol ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Bioengineering 2020Quote: ... Equal amounts of protein lysates (10-20 μg total protein) were subjected to 4–20% SDS-PAGE (Bio-Rad, Hercules, CA). Western analysis was carried out with primary antibodies against phosphorylated Smad1/5 (p-Smad1/5) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg of lysate was heat denatured for 3 minutes at 95°C for 3 minutes and resolved on a pre-cast 4-15 % SDS-PAGE gels (Bio-Rad) at 200V for 45 minutes in 1X running buffer (25mM Tris pH 8.3 ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of the protein samples were separated on 4-20% SDS-PAGE (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) at 20 mA and 250 V as the limiting voltage.
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer B + 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer D ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer C + 400 mM NaCl ...
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bioRxiv - Pathology 2021Quote: ... maximum power with 30 second intervals and the lysate obtained by ultracentrifugation at 100,000 g for 60 minutes, 4°C (CS 100FNX, HITACHI) resuspended in PBS and the concentration was measured by Bio-Rad protein assay dye reagent concentrate (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... quantified by micro-BCA assay (Pierce) in duplicate extracts) on 4-20% gradient Bis-Tris polyacrylamide stain-free pre-cast gels (Bio-Rad) in denaturing conditions ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of neuronal lysate and corresponding mHTTex1 seed were boiled for 5 min with sample loading buffer and loaded into a 4-20 % polyacrylamide gel (SDS-PAGE, Criterion Bio-Rad) to detect monomeric endogenous HTT or by semi-denaturing detergent agarose (1.5% ...