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Citations for Bio-Rad :
4051 - 4100 of 4802 citations for 6 Hydroxy 2 2 pyridinyl 4 3H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad®) for separation (200 V ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were subjected to westernblot analysis using precast polyacrylamide gels (10-20% Tris-HCl or 4-12% Bis-Tris, Bio-Rad). For immunoprecipitation (IP ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were washed and boiled in sample buffer and resolved on 10% SDS-PAGE or gradient gels (4 – 20%, Bio-Rad) and electro-transferred to PDVF membrane ...
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bioRxiv - Cancer Biology 2020Quote: ... 30 μg of protein was loaded into each lane of a TGX-stain-free pre-cast 4–20% SDS gel (Biorad, 4568094), total protein was quantified upon stain-free gel imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amount of proteins from each lysate were subjected to polyacrylamide gel (4-10 %) electrophoresis (PAGE) in Mini-PROTEAN Tetra cell apparatus (Bio-Rad). PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad). The column bed was washed with 40 ml of a wash buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 1000xg to pellet the beads and the supernatant was loaded into a Mini-PROTEAN TGX 4-20% (w/v) gradient gel (Bio-Rad) to run for 45 minutes at 150V ...
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bioRxiv - Neuroscience 2021Quote: ... separated on a 4–12 % SDS/PAGE gel and then transferred using the Transblot®-Turbo™ Transfer system (Bio-Rad). After 1 hour blocking in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... and boiled for 5 min prior to loading on 4-20% SDS-PAGE gradient gels (Mini-PROTEAN TGX precast gels; Bio-Rad). Precision Plus Protein Unstained Protein Standards (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... boiled and electrophoresed on 12% vol vol−1 and 4% vol vol−1 acrylamide SDS-PAGE gels (Bio-Rad Laboratories, Canada) on MiniProtean® camera (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of total protein (20-60 µg per lane) was resolved by SDS-PAGE using Mini Protean TGX precast gels 4% to 20% (Bio-Rad). Immunoblotting by semi-dry transfer (Bio-Rad Trans-Blot SD semidry transfer cell ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg per lane of total protein samples were separated on 4-12% Bolt Bis-Tris Plus Gels and blotted onto polyvinylidene difluoride membrane (Bio-Rad) using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... or the eluates were separated on a 4-15% gradient sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pre-casted gel (Bio-Rad). For Mn2+-Phos-tag SDS-PAGE gel ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were eluted in the denaturing mode and run in a 4-20% Mini-Protean TGX stain-free precast protein gel (Bio-Rad) at 200V for 30 min and stained by Imperial protein stain (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... SDS-Page was performed to resolve protein by molecular weight using 10 micrograms of hippocampal lysate from each sample in 4-20% acrylamide pre-cast gels (Biorad, 4568094). Resolved protein was transferred to PVDF membrane and blocked with 5% BSA solution in PBST ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were normalised after Bradford protein quantification and resolved on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad), transferred to nitrocellulose and blocked in 5% milk in PBS-T (PBS + 0.05% Tween 20) ...
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bioRxiv - Microbiology 2021Quote: The eluted IP samples were separated by molecular weight using SDS-PAGE (precast 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels, 10 well, 50 μl; Bio-Rad) at 160 V for 10 min ...
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bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
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bioRxiv - Microbiology 2021Quote: Protein samples were resolved by SDS-PAGE using 4-12% Bis-Tris NuPAGE precast gels with MES running buffer alongside the Precision Plus prestained protein standard (Bio-Rad). Proteins were transferred to nitrocellulose membranes following SDS-PAGE using an iBlot 2 Dry Blotting System (Thermo) ...
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bioRxiv - Microbiology 2020Quote: ... or immunoprecipitation products were denatured by boiling for 10 minutes and run on a 4-12% Gradient Bis-Tris Gel (Bio-Rad) before being transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... resolved on Novex 4–20% tris–glycine gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad). As a loading control ...
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bioRxiv - Microbiology 2019Quote: ... samples were boiled for 10 minutes and loaded on 4–20% Mini-PROTEAN TGX gradient gels (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Molecular Biology 2021Quote: ... prepared samples (15 μL) were pipetted onto gradient SDS-polyacrylamide gels (4%–15% Criterion TGX Stain-free gels; Bio-Rad Laboratories), and electrophoresis commenced at 180 V for 50 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The proteins of an 0.1 OD600 equivalent quantity of cells were separated by SDS-polyacrylamide denaturing gel electrophoresis using precast TGX 4-15% gradient gels (Bio-Rad). The proteins were then transferred to a 0,2µm nitrocellulose membrane using Trans-Blot Turbo RTA Transfer Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Ref: 4568085) at 160V ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 50 and 100 µg of total protein was loaded onto a gradient (4-15%) polyacrylamide gel (BioRad, Hercules, CA, USA). Proteins were then transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amounts of protein were then separated by SDS-PAGE on 4-20% polyacrylamide gels (Bio-Rad Laboratories, Richmond, VA, USA) at 200V for 40min ...
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bioRxiv - Neuroscience 2020Quote: ... Equivalent total protein amounts from BH and EVs were separated on 4−15% stain-free pre-cast SDS-PAGE gradient gels (Bio-Rad) under non-reducing conditions and transferred onto PVDF membranes (Sigma Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... Electrophoresis was performed at 200 V for 30 min on a 4–20% polyacrylamide gel (Mini-PROTEAN® TGX™, BioRad) in SDS running buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2020Quote: Organoids fixed in 4% w/v PFA and immunostained were embedded in 1% w/v low melting agarose (Bio-Rad Laboratories) solution and placed in an Eppendorf tube ...
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bioRxiv - Biophysics 2021Quote: ... fPanx1ΔC purity was assessed by SDS–polyacrylamide gel electrophoresis (PAGE) using 4-20% pre-cast gradient Tris-glycine gel (Bio-Rad) stained with Simply Blue (SimpleBlue Safe Stain ...
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bioRxiv - Microbiology 2021Quote: ... The mCherry-fusion lysate (input) and proteins eluted from the beads were separated on a 4–20% TGX SDS-PAGE gel (Bio-Rad) and transferred to a PVDF membrane for immunoblot analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was established using BCA kits (Pierce) and 10µg of protein was loaded into each well of a 4-20% pre-cast gel (BioRad, MiniProtean TGX), flanked with ladder at either end of the gel (PageRuler ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled for 5 min and the proteins were separated in 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-RAD) and electroblotted to nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2020Quote: ... They were lysed in 2x Laemmly buffer (4% SDS, 20% glycerol, 120mM Tris) and resolved by SDS-PAGE gel (BioRad, Country). Proteins were transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... were separated via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast 4-20% gradient polyacrylamide gels (Mini-Protean TGX, Bio-Rad). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... size-separated by SDS-PAGE in 4–20% Criterion TGX Precast gels and transferred using Trans-Blot Turbo Midi Nitrocellulose Transfer Packs (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline/0.5 % Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were then clarified through centrifugation (14’000 g, 20 minutes, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of total protein were resolved in 4-20% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo transfer system (Bio-rad). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were resolved by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) using 4-20% precast gel (Biorad, Hercules, CA). Proteins were transferred by semi-dry transfer onto a PVDF (polyvinylidene fluoride ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were incubated overnight at 4°C with primary antibodies and then probed with secondary antibodies before ECL development and imaging (Bio-Rad). Primary antibodies used for immunoblotting are as follows ...
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bioRxiv - Biochemistry 2022Quote: ... cell density was between 4-5 × 106 cells/mL with >95% viability as determined using a TC20 automated cell counter (Bio-Rad). Cells were diluted with warm Expi293 Expression Media to 2 × 106 cells/mL in 42.5 mL ...
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bioRxiv - Bioengineering 2022Quote: ... Approximately 20 μL of the denatured phage was loaded in a 4-20 % gradient gel (Mini-PROTEAN® TGX Stain-Free gel, Biorad) and separated by electrophoresis at 120 V ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated on ExpressPlus™ PAGE Ready gels 4 - 20% (A2S, M42015) and transferred on the cellulose membrane (300 mA, 90 min) using a BioRad blotting device (BioRad, USA). After overnight blocking in 5% milk in TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary and HRP-secondary antibodies in TBST buffer with 5% milk (RT for 1 hour or overnight at 4 °C) and revealed with an ECL solution (BIO-Rad) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...