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Citations for Bio-Rad :
4101 - 4150 of 4242 citations for 6 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... plates were coated with 100 μl of either 1 μg/ml of rh-TfR1-ECD (purified in-house) or liver purified ferritin (Bio-Rad, 4420-4804) overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4° with primary antibodies diluted in blocking solution as follows: mouse α-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360); mouse α-SP56 (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... or POPC:palmitoyloleoylphosphatidylserine (POPS (85:15) at a 1:200 protein to lipid ratio by dialysis in the presence of Bio-Beads (Bio-Rad, Hercules, CA) into metal free buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... and α-TUBULIN (1:4000) were detected using SuperSignal™ West Pico or West Femto Chemiluminescent Substrate chemiluminescence in ChemiDoc MP imaging system (BioRad, Berkeley, USA). The densitometry analyses were performed using Bio-Lab Software ...
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Cell Biology 2019Quote: ... cells from a 10 cm dish were pelleted and lysed with 30 µl lysis buffer (20 nM Tris-HCl, 150 mM NaCl, 1% Triton X-100 (Bio-Rad, Hercules, CA), 20 mM beta-glycerol phosphate and protease inhibitors cocktail ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
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bioRxiv - Microbiology 2021Quote: ... The quality of extracted DNA samples was also checked by loading one μl of DNA in 1% Agarose Gel (Bio-Rad, California, USA). The extracts were stored at -20 C until use.
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bioRxiv - Developmental Biology 2020Quote: ... protein samples were boiled with β-mercaptol and ran in SDS-PAGE gels casted from 30% Acrylamide/Bis solution 29:1 (Bio-rad, 1610156) using the Mini-PROTEAN® system (Bio-rad) ...
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bioRxiv - Genomics 2019Quote: ... the integrity and quantity of total RNA was confirmed by 1% agarose gel electrophoresis and the Experion™ automated electrophoresis system (Biorad Laboratories, Australia).
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bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The total RNA (1 μg) from each sample was reverse transcribed into cDNA using the iScript cDNA select Synthesis Kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNAs were subjected to PCR analyses using specific primers (The primer sequences are listed in Table 1) on an amplification detection system (Bio-Rad, Hercules, USA). β-actin served as a housekeeping gene ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was diluted 1:40 in water before using 1 μL as template in 10 μL qPCRs with iTaq™ Universal SYBR Green Supermix (Catalog# 1725121, Bio-Rad, USA) and 400 nM each primer ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and OD600 were measured from bacterial cultures diluted 1:10 using a Synergy H1 plate reader (Bio-Tek, Winooski, WI) and a Bio-Rad spectrophotometer (Bio-Rad, Hercules, CA), respectively ...
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bioRxiv - Immunology 2022Quote: ... and 1 µl of respective primer pairs (200 nM final concentration) were mixed and distributed into 96-well qPCR plate (Bio-Rad; cat. #12001925). Droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were boiled 1 min and subjected to SDS-PAGE using a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel (Bio-Rad Ref. 4568085). After SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: 100 microlitres of the secreted fractions and 100 µl of 1:20 diluted (in PBS) lysates from the secretion assays were loaded into a bio-blot microfiltration blotting system (BioRad. Cat. Nº: 1706545). Samples were left to flow through a 0.45 µm nitrocellulose blotting membrane (Amersham Protran ...
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bioRxiv - Cell Biology 2022Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg of RNA was used as input for cDNA synthesis using the iScript cDNA synthesis kit (Bio-rad, cat. no. 1708890) or Superscript III (Invitrogen cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times in TBST prior to incubation with secondary antibody (1/5000 dilution horseradish peroxidase (HRP)-conjugated anti-mouse (Bio-Rad, 170-6516) or anti-rabbit (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA). qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Immunology 2023Quote: ... The expression of immune system markers were mostly studied with inmunohistochemistry using the following antibodies: mouse anti rat CD68 (dilution 1:300 BIORAD, ref. MCA 341R) for monocytes/macrophages ...
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bioRxiv - Microbiology 2023Quote: The resulting amplicons and their corresponding lengths were visualized and documented via gel electrophoresis and analysis (Quantity 1 software, Bio-rad, CA, USA).
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM DTT) to a final KOAc concentration of 75 mM and incubated with hydroxyapatite Bio gel HTP (Bio-Rad #130-0402) for 45 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue) were reverse transcribed to cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891) and stored at −20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... The purified RNA was quantified by NanoDrop and 1 µg of RNA for each sample was used to generate cDNA using the iScript cDNA synthesis kit (Biorad cat. no. 1708890). Real-time PCR was performed with PowerUp SYBR Green Master Mix (Applied Biosystems cat ...
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bioRxiv - Immunology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, United States). The limit of detection was calculated to be 10 copies per reaction.
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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bioRxiv - Molecular Biology 2023Quote: ... and optic density of the bands corresponding to mouse PRM1 and PRM2 was quantified using Quantity One 1-D analysis software (BioRad, Hercules, CA, USA) to calculate the PRM1/PRM2 ratio ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Cell Biology 2022Quote: ... in 1× TBS for 5–10 min at room temperature to visualize DNA and mounted with FluoroGuard anti-fade reagent (Bio-Rad, Hercules, CA).
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1:10 dilution of cDNA was mixed with SsoAdvanced Universal SYBR Green Supermix (cat no 1725271, Bio-Rad, Hercules, CA) and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... total RNA (1 µg) was taken from the cDNA preparation using iScript™ cDNA Synthesis Kit (#1708891, Bio-Rad Laboratories, Hercules, CA, USA). Prior to cDNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Diluted cDNA (1:50) was used as a template for quantitative PCRs using iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Real-time detection of fluorescence emission was performed on a CFX384 Real-Time System (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...