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Citations for Bio-Rad :
351 - 400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... ice-cold purification buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl) in a small gravity column (Mini bio spin, Biorad). 8 to 10 mL apoplastic fluid containing the His-tagged proteins was loaded twice onto the resin ...
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bioRxiv - Physiology 2024Quote: ... All primers used in this study were mRNA specific and designed for qPCR (Bio-Rad CFX Manager, USA) using ChamQ Universal SYBR qPCR Master Mix (Q711-02 ...
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bioRxiv - Plant Biology 2024Quote: ... α-StrepTag antibodies (Biorad). After transferring to nitrocellulose membrane ...
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bioRxiv - Plant Biology 2024Quote: ... one μg total RNA was reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... in a CFX Connect™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). The cDNA samples were normalized with b-actin as the reference gene using the primers SlActinF 5’-GTCCCTATTTACGAGGGTTATGCT-3’ ...
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bioRxiv - Plant Biology 2024Quote: ... After membrane imaging using GelDoc Go Imaging System (Bio-Rad, USA), the measurement of the colour intensity of the dots was carried out using Image Lab Software v ...
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bioRxiv - Plant Biology 2024Quote: ... visualisation of bands was performed using BCiP and NBT at a concentration described above in the GelDoc Go Imaging System (Bio-Rad, USA). For qualitative and quantitative analysis ...
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bioRxiv - Plant Biology 2024Quote: ... according to the manufacturer’s instructions using either a ChemiDoc Imaging System (Bio-Rad) or conventional X-ray films.
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bioRxiv - Plant Biology 2024Quote: ... Horseradish peroxidase activity was detected with a Clarity western ECL substrate reaction kit (Bio-Rad) and a ChemiDoc Imagers (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated in 10% (w/v) SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Bio-Rad). Membranes were blocked in 5% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad, 39 cycles).
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bioRxiv - Plant Biology 2024Quote: ... proteins were visualized using the Bio-Rad Clarity™ Western ECL kit and the ChemiDoc Imaging System (Biorad).
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bioRxiv - Plant Biology 2024Quote: ... gels were electro-blotted onto a nitrocellulose membrane for 30 min at 200 mA using a semidry transfer apparatus (Bio-Rad, California, USA). Membranes were blocked with 3% non-fat dry milk in PBS for 2 h and then incubated with ToBRFV-specific laboratory-produced primary antibodies (1:4000 ...
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bioRxiv - Plant Biology 2024Quote: ... An AP substrate (NBT/BCIP, Bio-Rad, California, USA) was used to detect ToBRFV CP ...
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bioRxiv - Plant Biology 2024Quote: ... 10 ng of cDNA was used for qPCR with the CFX384 Touch real-time PCR detection system (Bio-Rad) and ONEGreen® Fast qPCR Premix (Ozyme) ...
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bioRxiv - Plant Biology 2024Quote: ... the ORF was PCR amplified from Arabidopsis thaliana (ecotype Columbia-0) cDNA using iProof DNA polymerase (Bio-Rad), specific primers (ORF-MAP3K19-F ...
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bioRxiv - Plant Biology 2024Quote: ... digital photos of infected seedlings were taken with ChemiDoc XRS + Imager (BioRad). Relative bioluminescence was quantified as described previously (Furci et al. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Proteins (10-50 µg) were loaded onto 10% sodium dodecyl sulfate polyacrylamide gel (BioRad), separated by electrophoresis (SDS-PAGE) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The colour intensity (absorbance) was determined using a microplate reader (model 550, BioRad) at 450 nm ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and chemiluminescence was detected with a digital imaging system (ChemiDoc XRS+ system with image lab software, BioRad). Quantification was performed by Image Lab software version 6.0 (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Synthetic Biology 2024Quote: The samples (1 µL) were subjected to SDS-PAGE and transferred to a nitro cellulose membrane (Bio-Rad Laboratories, CA, USA). The membrane was blocked using 3% skim milk in PBST (137 mM NaCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... Electrocompetent cells were transformed using electroporation cuvette (2mm gap, Bio-rad) and an electroporator set to 2500 V (Eporator ...
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bioRxiv - Plant Biology 2024Quote: ... 6.1 (Bio-Rad, USA). The heat map was prepared using Microsoft tools in which colour intensity is proportional to a numerical value ...
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bioRxiv - Plant Biology 2024Quote: pTRV1 and pTRV2-Del/Ros-1 harboring the SlATG7- or SlATG2-derived fragments were introduced into Agrobacterium strain GV3101 using electroporation (BIO-RAD). A 5 mL culture was grown overnight at 28°C in a medium containing kanamycin ...
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bioRxiv - Plant Biology 2024Quote: ... 6.1 (Bio-Rad, USA). Three independent analyses were carried out.
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bioRxiv - Plant Biology 2024Quote: ... Goat anti-mouse Kappa Light Chain antibody (Bio-Rad, #105001G) and Goat Anti-Rabbit (IgG (H + L)-HRP Conjugate #1706515 ...
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bioRxiv - Plant Biology 2024Quote: ... followed by a 1 h incubation with anti-mouse IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized from total RNA using the iScript Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA) with 1 µg of RNA in a 20 µL reaction volume ...
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bioRxiv - Physiology 2024Quote: ... Fluorescent signals were detected using a ChemiDoc MP imaging system (Biorad).
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bioRxiv - Physiology 2024Quote: ... proteins were visualized using ClarityTM Western ECL Substrate (BioRad) and imaged with a ChemiDocTM XRS+ Molecular Imager (BioRad) ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined by Bradford assay (Bio-Rad). Twenty micrograms protein of tissue homogenates were mixed with 4× LDS sample buffer and subjected to SDS-PAGE gel electrophoresis ...
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bioRxiv - Physiology 2024Quote: ... 5 ug (BAT) or 10 ug (ingWAT) of protein was separated on a 12% polyacrylamide gel (Mini-PROTEAN® TGXTM, BioRad). Proteins were then transferred to a PVDF membrane (BioRad ...
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bioRxiv - Physiology 2024Quote: ... and imaged with a ChemiDocTM XRS+ Molecular Imager (BioRad). The obtained images were analyzed with Image LabTM software (BioRad) ...
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bioRxiv - Physiology 2024Quote: ... Using a wet transfer system (Bio-Rad). The membranes were blocked with 5% nonfat dry milk for 1 h and then incubated with the following antibodies ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined by Bradford assay (500-0006, Bio-Rad, Hercules, California, US).
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bioRxiv - Plant Biology 2024Quote: ... or observing the autofluorescence using the BioRad Gel Imager (Bio-Rad, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA) following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and the reaction was performed using a CFX96 Real time system (Bio-Rad, USA). Primer sequences are shown in Table S2.
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bioRxiv - Plant Biology 2024Quote: ... consisting of 7 μL Sso Advanced Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA, USA), 0.28 μL of each primer (10 μM) ...
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bioRxiv - Plant Biology 2024Quote: ... and a PCR (C1000 TouchTM Thermal Cycler, Biorad Laboratories, Inc.). First-strand cDNA synthesis of 500 ng of RNA in a final volume of 20 μL was performed using iScript cDNA synthesis kit (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2024Quote: qPCR was conducted in a 20 µl reaction of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), dilute cDNA ...
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bioRxiv - Plant Biology 2024Quote: ... After mixing with 4x Laemmli sample buffer (Bio-Rad 1610747) containing 2-mercaptoethanol and boiling at 70°C for 10 min to stop the proteolytic reaction ...
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bioRxiv - Plant Biology 2024Quote: ... Thermal denaturation temperatures were obtained in a CFX96 touch real-time thermal cycler (Bio-Rad) with an optical reaction module by ramping the temperature between 20 and 95 °C at 1 °C per minute ...
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bioRxiv - Plant Biology 2024Quote: ... Data were analysed with CFX Manager Software V3.0 (Bio-Rad) and plotted using PRISM version 9.0 (www.graphpad.com).
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bioRxiv - Plant Biology 2024Quote: ... anti-rabbit (Bio-Rad, #1706515, dilution 1:10000), and anti-goat (Agrisera ...
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bioRxiv - Plant Biology 2024Quote: ... and a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Plant Biology 2024Quote: ... according to the manufacturer’s instructions with either ChemiDoc Imaging System (Bio-Rad) or conventional X-ray films.
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bioRxiv - Plant Biology 2024Quote: ... Gels were stained with the Bio-Safe™ Coomassie Stain solution (Bio-Rad 1610786) according to the manufacturer recommendations ...
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bioRxiv - Plant Biology 2024Quote: About 15 µg of proteins were separated on SDS-PAGE gels (Bio-Rad 3450009) at a constant voltage of 200V for 12 min ...