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Citations for Bio-Rad :
501 - 550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Protein concentrations were measured using a Protein Assay Dye Reagent Concentrate kit (#5000006, Bio-Rad) with bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: ... and the mixture was treated in MicroPulser Electroporator (Bio-Rad) at the pulse intensity of 1.8 kV and resulting pulse length of 1.3-1.5 ms ...
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bioRxiv - Bioengineering 2024Quote: ... The absorbance of each solution was measured at a wavelength of 450 nm using a UV–vis microplate reader (Bio-Rad, Philadelphia, PA).
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bioRxiv - Bioengineering 2024Quote: ... The working plasmids were added to 80 µL competent cells in a pre-cooled 0.1-cm MicroPulser Electroporation Cuvette (Bio-Rad) and the mixture was treated in MicroPulser Electroporator (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... 1x Dream Taq buffer A and 1 μL sample DNA in a total reaction volume of 10 uL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Bioengineering 2024Quote: ... Transformation of yeast cells by electroporation was carried out as in [27] with a Gene Pulser electroporator (Bio-Rad).
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bioRxiv - Bioengineering 2024Quote: ... separated on a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad), and transferred to a PVDF membrane by using CAPS-based transfer buffer ...
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bioRxiv - Bioengineering 2024Quote: ... and the image was captured by ChemiDoc Imaging System (BioRad). The following primary antibodies were used ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR reactions were carried out with the SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) in a Bio-Rad CFX96 apparatus and run by duplicate in two independent amplifications.
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bioRxiv - Bioengineering 2024Quote: ... Target genes and transcripts were quantified by qPCR using a CFX Opus 96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA) in 20 µL reaction mixtures containing 10 µL of SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... in 20 µL reaction mixtures containing 10 µL of SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), 300 nM of each primer ...
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bioRxiv - Biophysics 2024Quote: ... Mpro was exchanged into the buffer surrogate by applying two cycles of centrifugal gel filtration (Biospin mini columns, 6000 MWCO, Bio-Rad). nsp16 and nsp7-11 were exchanged by six rounds of dilution and concentration in centrifugal filter units (Amicon Ultra ...
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bioRxiv - Biophysics 2024Quote: SDS-Page was performed with a 4%-12% gradient acrylamide Bis-tris gel with XT MES running buffer (Bio-Rad Laboratories). Both constructs nsp7-11C and nsp7-11N were mixed at 36 µM with 14 µM Mpro and incubated at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Liposomes were also prepared as described previously (76) and were incubated with 10% (v/v) Chelex beads (Bio-Rad, Hercules, CA) overnight at 4 °C to remove residual Ca2+ ...
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bioRxiv - Biophysics 2024Quote: ... The mixtures were incubated for 1 h at RT and then BioBeads (BioRad) were added to each sample and incubated for 2 more hours under constant agitation to remove the detergents ...
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bioRxiv - Biophysics 2024Quote: ... containing 20 mM MgCl2 and exposed to a thermal annealing ramp (65 °C, 15’; 58-53 °C, 1 h/°C; stored at 20 °C) in a Tetrad2 (Bio-Rad) thermocycler ...
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bioRxiv - Biophysics 2024Quote: ... The coverslips were incubated with the primary antibody [anti-tyrosinated tubulin 1:150 (MCA77G, Bio-Rad)] in blocking solution (10% FBS with 0.05% Tween 20 diluted in cytoskeleton buffer overnight at 4ºC ...
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bioRxiv - Biochemistry 2024Quote: ... Transient transfections were performed with Transfectin reagent (Biorad, 1703352). HeLa cells were obtained from ATCC (CCL-2) ...
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bioRxiv - Biochemistry 2024Quote: ... mother liquor and fluorinated oil (QX200™, BioRad) which acts as the carrier phase ...
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bioRxiv - Biochemistry 2024Quote: ... were transiently over-expressed with TransFectin reagent (Bio-Rad, 1703352) in a 24-well plate format ...
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bioRxiv - Biophysics 2024Quote: ... Bio-Beads SM-2 resin (Bio-Rad) was added into the mixture ...
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bioRxiv - Bioengineering 2024Quote: ... into Econo-Pac® Chromatography Columns (BIO-RAD) to bed volume 10 mL ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane with the blotted proteins was blocked with EveryBlot Blocking Buffer (BioRad) for 2 h and then incubated with a primary antibody at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... gene and the HIV-1 Psi vector sequence were performed using respectively custom qPCR HEX assay and custom qPCR FAM assay (Bio-Rad, Marnes-la-Coquette, France) with previously published primer sequences.48 qPCR reactions were performed using 200 ng of gDNA and the Master Mix Taqman Universal II (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... All reactions were performed using the CFX Opus 96 Dx System (Bio-Rad). A standard curve was used to convert Ct values to copy numbers by amplifying serial dilutions of a unique plasmid containing equimolar ratios of the ALB and PSI target sequences.
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bioRxiv - Bioengineering 2024Quote: ... The proteins were transferred onto a 0.45 µm nitrocellulose membrane at 75 V for 90 min using a transfer assembly (BioRad Laboratories Inc.). The membrane was washed with 0.1% Tween 20 containing Tris-buffered saline pH 7.4 (T-TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein for each sample was determined using the DC Protein Assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Colonies that were identified as expressing mCherry based on fluorescence at 670 nm (Cy5 channel) using a Chemidoc MP Imaging System (Bio-Rad, Mississauga, Ontario, Canada) were streaked onto M9 glucose plates supplemented with 50 μg/mL gentamycin and 100 μg/mL ampicillin until pure cultures were obtained.
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bioRxiv - Bioengineering 2024Quote: Protein concentration was measured (BioRad, BCA kit) prior to quantifying expression of myelination and attachment markers using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was reverse transcribed to cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). qPCR was conducted using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR was conducted using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Hercules, CA) and the following primers (IDT ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were exposed after addition of ECL (170-5060, Bio-Rad, Hercules, CA). Tubulin was detected with hFAB™ Rhodamine Anti-Tubulin Primary Antibody (12004166 ...
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bioRxiv - Bioengineering 2024Quote: ... in 1X Tris Buffered Saline with 1% Casein (Bio-Rad, Hercules, CA). Secondary α-mouse HRP (A6154 ...
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bioRxiv - Bioengineering 2024Quote: ... Tubulin was detected with hFAB™ Rhodamine Anti-Tubulin Primary Antibody (12004166, Bio-Rad, Hercules, CA) at 1:3000 dilution.
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bioRxiv - Neuroscience 2024Quote: ... Precision Plus ProteinTM KaleidoscopeTM Prestained Protein Ladder (Bio-Rad) was used as a molecular weight marker ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with appropriate secondary antibodies before developing with Perkin-Elmer ECL Western Lightning Plus solution and visualized using a Chemidoc imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... Images were acquired with ChemiDoc Imaging Systems (Bio-Rad Laboratories, Inc).
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bioRxiv - Cancer Biology 2024Quote: ... Optical density was measured at 450 nm using a microplate spectrophotometer (Bio-Rad). Experiments were performed in triplicate.
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR was performed using manufacturers’ instructions for SSO advanced SYBR green kit (Biorad) on ABI-qPCR system using equal amount of template (50–100 ng cDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to PVDF membranes in 1X transfer buffer (Biorad) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR program was run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (#1725121, Bio-Rad, Hercules, CA,) using human Il6 and mouse B2m ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, 1725121). Each reaction proceeded at 50 °C for 2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were prepared for SDS-PAGE in Laemmli sample buffer (BIO-RAD) and heated to 70 °C for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were blocked with 5% nonfat dry milk (NFDM, BIO-RAD) TBS with Tween-20 (TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... and then transferred onto a nitrocellulose membrane (Bio-Rad Laboratories, Inc.). The membrane was blocked with SuperBlock™ Blocking Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein was visualized using an ECL substrate kit (BIO-RAD) and imaged with a ChemiDoc-It2 system.
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bioRxiv - Cancer Biology 2024Quote: ... nonfat dry milk (NFDM) and ECL substrate kit were purchased from Bio-rad (CA, USA). Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA was prepared using Superscript First Strand Synthesis System for RT-PCR Kit (Biorad) following manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and ChemiDoc MP Imaging System (Bio-Rad) CCD camera ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins bound to Dynabeads were eluted with 2 x Laemmli sample buffer (Bio-Rad) and subjected to immunoblot analysis ...