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Citations for Bio-Rad :
251 - 300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... and total protein content was quantified using DC Protein Assay (Biorad, 5000113). 15 to 30 μg of total protein lysate were resolved on SDS-PAGE gel and transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Biophysics 2024Quote: ... The immunoprecipitated elutions were run on precast 4-20% gradient gels (Midi Criterion™ TGX™, Biorad) and stained with Coomassie blue for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... of the IDH1 R132H mutation were determined in 69 tumor sections from case one and the patient’s blood using the PrimePCR IDH1 R132H mutant assay and the QX100 Droplet Digital PCR system (Bio-Rad Inc., Hercules, CA). An initial serial dilution of a positive control was performed to optimize the input concentration of genomic DNA from each section and to assess the reliability of the assay ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was quantitated by using the Bradford assay (Bio-Rad). Between 10 to 30 µg of protein were prepared in Laemmli buffer boiled for 5 min and proteins size separated by SDS-PAGE using NuPAGE 10% Bis-Tris 1.5 mm gels (Life Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... REVERT total protein stain (BioRad) was used as indicated by manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... Samples (10ug) were separated on 10% SDS-PAGE gel (161-0183, BioRad), then transferred to a membrane ...
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bioRxiv - Cell Biology 2024Quote: ... qRT–PCR was performed using iTaq Universal SYBR Green SuperMix (Bio-Rad, #1725121). SYBR Green real-time PCR was performed in triplicate on the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Key Resources Table ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 1ug RNA using iScript reverse transcription kit (Bio-Rad, #1708891) and diluted 1:3 in water ...
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bioRxiv - Biophysics 2024Quote: ... or for 5 min with Clarity Western ECL Substrate (Biorad, 1705061).
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bioRxiv - Developmental Biology 2024Quote: ... All membranes were blocked in Everyblot blocking buffer (12010020, Bio-Rad) for 1 h and probed overnight with primary antibodies in blocking buffer at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Data were analyzed and 95% Poisson confidence intervals were calculated using QuantaSoft software (Bio-Rad Inc., Hercules, CA).
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bioRxiv - Physiology 2024Quote: ... T3 was extracted from supernatants and purified through solid-phase chromatography using 200 – 400 anion exchange chloride resin (Bio-Rad, 140-1251) in Poly-Prep chromatography columns (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... in Poly-Prep chromatography columns (Bio-Rad, 731-1550). Columns were developed with 70% acetic acid (Spectrum ...
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bioRxiv - Cell Biology 2024Quote: ... 96-well PCR plates were sealed with peelable foil heat seals at the PCR plate sealer machine (PX1TM, Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... or CFX Duet Real-Time PCR System (Bio-Rad, CA, USA) with THUNDERBIRD Next SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Cell Biology 2024Quote: ... Technical replicates of 20 ng (based on RNA concentration) were used to measure Ct on a BioRad CFX96 thermocycler with iTaq UniverSYBR Green SMX (BioRad 1725125) in 20 µl reactions ...
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bioRxiv - Cell Biology 2024Quote: ... and quantified with the DC Protein Assay (BioRad 5000112) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on 12% SDS-polyacrylamide (29:1) or 4-15% precast polyacrylamide mini-gels (Bio-Rad) and electrotransferred on nitrocellulose membranes ...
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bioRxiv - Cell Biology 2024Quote: ... ChIP-qPCR reactions were run on a BioRad CFX96 thermocycler with iTaq UniverSYBR Green SMX (BioRad 1725125) in 20 µl reactions and quantitated relative to input dilutions ...
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bioRxiv - Cell Biology 2024Quote: ... then transferred to the nitrocellulose membrane (0.2 µm, Bio-Rad) by the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... using a turbo transfer system (Bio-Rad). Membranes were blocked for 1 hr in TBST (Tris-buffered saline with 0.1 % Tween 20 ...
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bioRxiv - Neuroscience 2024Quote: ... The peroxidase reaction for AffiniPure antibodies was developed with Clarity™ Western ECL substrate (Bio-Rad, 1705061). Imaging was performed using either an Odyssey Fc imaging system (Li-Cor ...
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bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was performed in triplicate on a CFX384 Real Time System C1000 Thermal Cycler (Bio-rad) using Sso Fast EvaGreen Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were boiled for 5 min in Laemmli sample buffer before being separated at 200 V for 40 min using TGX stain-free precast gels (Bio-Rad). Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Imaging was performed using either an Odyssey Fc imaging system (Li-Cor) (quantified using Image StudioTM software) or a Molecular ChemiDocTM Touch Imaging System (Bio-Rad) (quantified using Image Lab 6.0.1 software).
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bioRxiv - Plant Biology 2024Quote: ... using iTaq Universal SYBR Green Supermix (BioRad) with at least three biological replicates each with three technical repeats ...
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bioRxiv - Physiology 2024Quote: ... using a CFX Opus Real-Time PCR System (Bio-Rad Laboratories). Primers used for qRT-PCR are listed in Supplemental table 1.
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR reactions were carried out in a CFX Connect Real-time PCR detection system (BioRad, Hercules, California, USA) using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... Plates were left to dry on top of a piece of Whatman paper before imaging on a Bio-Rad Gel Doc XR+ imaging system (Bio-Rad, 1708195EDU). hTERT-RPE1 cells are highly mobile and do not form distinct colonies ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were measured by Bradford assay using Protein Assay Dye Reagent Concentrate (Bio-Rad, 5000006). Samples were normalised to 0.5-1 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... using a Trans-blot Turbo system (Bio-Rad #1704150). After transfer ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred onto nitrocellulose membranes (Bio-Rad #1704158, #1704159) using a Trans-blot Turbo system (Bio-Rad #1704150) ...
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bioRxiv - Cell Biology 2024Quote: ... and then dried in a gel dryer (BioRad, model #583). Detection of the incorporated label was performed by autoradiography ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by SDS-PAGE (Bio-Rad, Mini-PROTEAN® Tetra cell, #1658000) and transferred onto nitrocellulose membranes (Bio-Rad #1704158 ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was conducted with iTaq-SYBR Green (Bio-Rad) and primers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for chemiluminescence detection with a Bio-Rad Molecular Imager Gel Doc XR System (Bio-Rad). Primary and secondary antibodies were diluted according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2024Quote: ... and imaged on GelDoc DOCTM XR+ Gel Documentation system (Bio-Rad). PCR oligonucleotide primers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and primers (Integrated DNA Technologies) using CFX 384 Touch Real-Time PCR detection system (Bio-Rad). We used following primers ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies conjugated to IR dyes (BioRad) were used for Western blots ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for developing the signal and membranes were imaged and analyzed using the ChemiDoc Imaging System with ImageLab software (Bio-Rad). Cell lysates from the p53 mutant Eµ-Myc lymphoma cell line (EMRK1172 ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins were separated by SDS-PAGE on 4-15% gradient gels (Biorad) and transferred on to PVDF membranes using the iBlot Dry Blotting system (Thermofisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments was separated on 20% denaturing acrylamide gel (5% crosslinker, 7 M urea, 1X TBE buffer) using Criterion™ cell apparatus (Bio-Rad) at 300 V for 40 to 60 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The resolved proteins were transferred onto nitrocellulose membranes (Amersham) through a Trans-Blot semi-dry transfer cell (Bio-Rad). Membranes were subsequently blocked in Tris-buffered saline plus 0.1% Tween (TBS-T ...
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bioRxiv - Immunology 2024Quote: ... 72°C for 5 minutes (BioRad, Hercules, CA). Genomic PCR was performed using the following primers ...
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bioRxiv - Bioengineering 2024Quote: ... Thermocycling and cycle threshold (ct) measurements was conducted on the CFX96™ qPCR instrument (Biorad). Gene expression levels were analyzed from ct values of the target genes and relative expression was quantified using the double-delta ct method.92 All primers used for qPCR are listed under Table S3.
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed with SsoFast™ EvaGreen® Supermix (Biorad) following manufacturer recommendations for both reaction setup and cycling conditions ...
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bioRxiv - Bioengineering 2024Quote: ... The 5nM dilution was further quantitated by qPCR on a BioRad CFX Connect Real-Time System (Bio-Rad Laboratories, Inc. CA).
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bioRxiv - Bioengineering 2024Quote: ... and run on flow cytometry (Bio-Rad Ze5). Data was plotted using Prism (GraphPad).