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Citations for Bio-Rad :
3901 - 3950 of 4939 citations for 4 Phenyl 3 buten 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Proteins were separated by SDS-PAGE on 4-15% gradient Mini-Protean TGX Stain-free gels (cat. no. 4568083, Bio-Rad), transferred onto PVDF membranes (cat ...
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bioRxiv - Molecular Biology 2023Quote: 10-20% of the samples containing immunoprecipitated ECTs for mass-spectrometry were subjected to SDS-PAGE in 4-20% CriterionTM TGXTM Precast Midi Protein gels (Bio-Rad) for visual assessment ...
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bioRxiv - Microbiology 2023Quote: ... The serial dilutions and supernatants of auxin- and vehicle-treated parasites of a single strain were loaded onto the same precast 4–15% gel (Bio-Rad). Subsequent immunoblotting steps were performed as described in “Immunoblotting” ...
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bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins ≤ 250 kDa were separated by SDS-PAGE electrophoresis using 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and transferred to Immobilon-P PVDF Membrane (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was quantified by Qubit and 15ug protein was run on a 4-20% Mini-PROTEAN(R) TGX Stain-Free Protein gel (Bio-Rad). Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... and Precision Plus Protein Unstained Standards (#1610363) was resolved by SDS-PAGE using precast 4-20% MiniProtean TGX Stain Free Gels (Bio-Rad). The blot in Fig ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie Blue.
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie blue dye.
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bioRxiv - Biochemistry 2023Quote: ... the clear supernatant was loaded on a 4–20% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad Laboratories, Inc., Hercules, USA) including PageRuler™ Plus Prestained Protein Ladder (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2023Quote: ... 10 µg (for FLN-DD experiment) of protein lysates for each treatment condition was fractionated in 4-15% Mini-Protean TGX protein gels (Bio-Rad) and transferred to nitrocellulose membrane using Bio-Rad Trans-Blot Turbo Transfer System with “Turbo Mixed MW” settings (constant 2.5A for 7 minutes) ...
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bioRxiv - Plant Biology 2023Quote: Protein samples of whole cell extracts (0.5 µg chlorophyll or 10 µg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) and blotted onto nitrocellulose membranes ...
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bioRxiv - Plant Biology 2023Quote: ... and 10 (V/V) % beta-mercaptoethanol for 5 minutes before being loaded into a 4-20% gradient SDS-PAGE gel (Biorad: 4561096) and run according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Mini-Protean TGX gradient gels (4561094, Bio-RAD) and stained with Coomassie Blue ...
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bioRxiv - Plant Biology 2023Quote: ... during 35 minutes at 200 V (Mini-PROTEAN® Tetra Cell, 4-Gel System, Bio-Rad laboratories Inc, Hercules, CA, USA) and were electroblotted onto 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
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bioRxiv - Systems Biology 2023Quote: ... Whole-cell lysates were separated on an SDS-PAGE gradient gel (4–15%) and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad). p21 and Cdk2 were detected in 15 μg of whole-cell lysates ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2022Quote: ... pulldown samples collected as described above were loaded on a 4-12% gradient Criterion XT Bis-Tris precast gel (Bio-Rad). The gel was fixed in 40% methanol and 10% acetic acid and subsequently stained for 1h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Biochemistry 2023Quote: 15µg was loaded on a 4-20% acrylamide gradient gel with 18 or 26-wells (Bio-Rad #5671094 & Bio-Rad #5671095) and separated for 20min at 100v followed by 150V for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were electrophoresed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) precast 4-20 % Mini-PROTEAN TGX gel (Bio-Rad) to separate the proteins.
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Protein (10 ug) from each sample was treated with ß-mercaptoethanol and subsequently electrophoresed on precast 4%–10% gradient gels (Biorad), as previously described (Alcantara et al. ...
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bioRxiv - Plant Biology 2023Quote: ... extracted proteins were diluted in SDS loading dye and incubated at 72°C for 10 min and were loaded to 4-20% Mini-PROTEAN TGX gels (Bio-Rad). Immunoblotting was performed with antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µl of input and 5 µl of IP samples were loaded and protein samples were separated on 4-20% Tris-glycine polyacrylamide gels (Bio-Rad) at 170 V for 1 hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 min in the DTT-containing sample buffer and separated by 4–20% Mini-PROTEIN TGX gels (Bio-Rad) and then transferred to the 0.2 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... resin with immunoprecipitated proteins were resuspended in SDS-loading buffer and electrophoresed on a 4-20% TGX stain-free gel (Bio-Rad) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: Protein samples were electrophoresed on home-made 4-16% Tris-glycine SDS-polyacrylamide gels and transferred to nitrocellulose membranes (0.45 µm, Bio-Rad, 1620115). Membranes were blocked in 5% non-fat milk ...
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bioRxiv - Biophysics 2023Quote: ... Samples were heated at 95 °C for 5 minutes to denature and reduce the proteins and were then separated by electrophoresis through 4-15% Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) with Tris/Glycine/SDS running buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 94°C before loading on polyacrylamide gels (4-15% Bis-Tris Gel, Bio-Rad). Proteins were separated by gel electrophoreses at 80 V and transferred at 90 V for 2 h on nitrocellulose membranes (Precast Protein Gels ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 95 ℃ for 10 minutes and resolved by SDS-PAGE using 4-20 % or 10 % precast gels (BioRad, 4561093), depending on the molecular weight of the desired protein ...
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bioRxiv - Cell Biology 2023Quote: ... The denatured protein samples (20 µg) were then separated on 4-20 % SDS-polyacrylamide gels using a Tris-glycine running buffer (Bio-Rad) and electroblotted onto nitrocellulose membranes (0.2 µm pore size ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein was denatured at 95°C for 5 min in 1x Laemmli buffer containing 5% β-mercaptoethanol and separated in 4-15% mini-PROTEAN TGX Precast Protein Gels (Biorad) by electrophoresis and blotted onto Nitrocellulose membrane (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were subjected to electrophoresis under reducing conditions on 4–15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and subsequently transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then clarified through centrifugation (14′000g, 20 min, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... For each protein of interest 10 µg of sample was loaded in a 4-15% Tris-Glycine gel (Bio-Rad, 4568083). The gel was transfered onto PVDF membrane (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl of lysate was separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), and transferred to a PVDF membrane for analysis using α-FLAG antibody (Sigma Aldrich #F3165 ...
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bioRxiv - Developmental Biology 2023Quote: ... 4% SDS and 0.005% Bromophenol Blue) for 5min prior to loading on a 4%-20% Mini-PROTEAN TGX gels (BioRad, #456-1094) and blotted using Trans-Blot Turbo Transfer Pack 0.2μm nitrocellulose (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR was employed to find the levels of quantification of mucin 1 and 4 mRNA transcripts according to protocol of the Real-Time PCR system (BioRad./ USA)[39] ...
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bioRxiv - Microbiology 2023Quote: ... The protein samples were separated in 4 −15% acrylamide gels by SDS-PAGE using a Bio-Rad Mini-Protean Tera cell system (Bio-Rad). At the same time ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
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bioRxiv - Biochemistry 2023Quote: 15µg was loaded on a 4-20% acrylamide gradient gel with 18 or 26-wells (Bio-Rad #5671094 & Bio-Rad #5671095) and separated for 20min at 100v followed by 150V for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... SDS-PAGE was performed using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (#4561084, Bio-Rad Laboratories, Inc, USA). Before and after the laser exposure ...
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Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... and heated at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE for 4 minutes (or until all the sample had entered the gel) at 200V (two wells per sample; 4-20% Mini-PROTEAN TGX precast protein gel, BIO-RAD), then stained with Coomassie blue for ten minutes before being washed 4 X with ddH2O (10 minutes each ...
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bioRxiv - Genetics 2024Quote: ... cDNA was diluted 1:4 with water and 2ul used as template for qPCR using 250nM primers using the SsoFast EvaGreen Supermix (BioRad 1725200) on an Applied Biosystems ViiA 7 Real Time PCR System ...