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Citations for Bio-Rad :
3851 - 3900 of 4939 citations for 4 Phenyl 3 buten 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: SDS-Page was performed with a 4%-12% gradient acrylamide Bis-tris gel with XT MES running buffer (Bio-Rad Laboratories). Both constructs nsp7-11C and nsp7-11N were mixed at 36 µM with 14 µM Mpro and incubated at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µg of nuclear extract in SDS loading buffer were loaded on a 4-15% precast polyacrylamide gel (Bio-Rad #4561083EDU) and Color-coded Prestained Protein Marker ...
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bioRxiv - Biochemistry 2024Quote: ... the beads were mixed with 1.5 × loading buffer and then loaded on a 4-15% pre-casted protein gels (BioRad; #456-1084) prior to transfer on PVDF membrane for western blotting using anti-Flag ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
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bioRxiv - Molecular Biology 2024Quote: Samples in 1X Laemmli loading buffer (generally representing 1-2 µL of replication reaction or 9 µL of a plasmid pulldown sample) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysates were separated by Bis-Tris sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4–20% precast polyacrylamide gel (BioRad, #4561096). The proteins were transferred to low fluorescence polyvinylidene difluoride (PVDF ...
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bioRxiv - Synthetic Biology 2024Quote: ... the maximum amount of protein that could be loaded to keep protein mass constant across all samples on a gel) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Physiology 2024Quote: ... Protein obtained was run on SDS-PAGE on precast polyacrylamide gel (Mini-PROTEAN TGX, 4%-20%; Bio-Rad Laboratories, Hercules, CA) and transferred to a polyvinylidene difluoride membrane via a semi-wet transfer equipment (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2024Quote: ... They were resolved by SDS–PAGE using a Bio-rad system and 4–15% Mini-PROTEAN TGX Precast Protein Gels (#4561086, Bio-rad) and transferred to nitrocellulose membranes (pore size 0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 min and run on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad). Resolved protein was transferred to nitrocellulose using Trans-Blot turbo transfer packs ...
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bioRxiv - Microbiology 2024Quote: ... 20µg of protein samples were heated in 1X Laemmli buffer at 95°C for 5 min and loaded on pre-cast 4-20% SDS-polyacrylamide electrophoresis gel (Bio-Rad). For the RIP experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were heated at 95°C for 5 minutes and then separated on a SDS-PAGE on TGX-stain free precast gel (4-15% of acrylamide gradient) and transferred on a nitrocellulose filter using a semi-dry transfer apparatus (TransBlot SD; Bio-Rad). Then ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for either semi-dry or wet transfer (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: Samples were prepared according to the western blot protocol and run through a 4-15% Mini-Protean Precast TGX Gel (Bio-Rad). The gels were then stained using the Pierce Silver Stain Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Samples were incubated at 95°C for 10 minutes and loaded onto SDS-polyacrylamide gels (Mini-Protean TGX gels 4-20%, Bio-Rad). Following gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and 30 µg of total protein extract were loaded on 10% acrylamide-bis-acrylamide gels or 4-20% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Proteins were separated by migration at 135 V in 1X Tris-Glycine-SDS buffer (Euromedex) ...
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bioRxiv - Molecular Biology 2023Quote: ... resin with immunoprecipitated proteins were resuspended in SDS-loading buffer and electrophoresed on a 4-20% TGX stain-free gel (Bio-Rad) for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... For each protein of interest 10 µg of sample was loaded in a 4-15% Tris-Glycine gel (Bio-Rad, 4568083). The gel was transfered onto PVDF membrane (GenScript ...
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bioRxiv - Biophysics 2024Quote: ... Econo-Pac 10DG Desalting Columns and 4-20% gradient Mini-PROTEAN®TGX™ Precast Gels were purchased from Bio-Rad Laboratories (USA) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved on 4–20% FastPAGE precast gels (Tsingke Biotechnology) and transferred to nitrocellulose membranes in a Trans-Blot Turbo machine (Bio-Rad). Membranes were blocked with 5% nonfat milk dissolved in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of extracts (quantified using Lowry assay) were subjected to SDS-PAGE analysis on 4-15% Criterion TX gels (Bio-Rad), transferred to nitrocellulose membranes using semi-dry transfer apparatus and Tans-Blot Turbo RTA Midi Nitrocellulose Transfer kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... This was accomplished using electroporation (260 mV for 30 ms; 4 mm-gap cuvette) via a Gene Pulser XCell system (Bio-Rad). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... boiled for 5 minutes at 95°C and loaded onto Mini-Protean TGX Stain Free Gels 4-20% (Bio-Rad, P4568096). The gels were transferred onto 0.45 μm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Pathology 2023Quote: ... and DNA fragment (amplification products containing homologous DNA fragments) in a 4-mm Gene Pulser and electrotransformed by Gene Pulser Xcell system (Bio-Rad). Transgenic parasites were selected with pyrimidine (1μM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... before being loaded on a SDS-PAGE gel (4–20% Criterion™ TGX Stain-Free™ Protein Gel, Bio-Rad #5678095). Western blot was then performed as described in the previous section.
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of diluted cDNAs were added to each PCR reaction containing the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725271) and primers at a final concentration of 300 nM each ...
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bioRxiv - Neuroscience 2023Quote: ... Ten µg of total proteins quantified by PierceTM BCA protein assay kit (ThermoScientific) were loaded on 4-15% gradient SDS–PAGE gels and transferred to nitrocellulose membranes (Trans-Blot turbo, Bio-Rad). Immunoblotting of rodent cultures was detected by chemiluminescence with clarity Western ECL on ChemiDoc Touch system with Image Lab software (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD) and then transferred to the 0.2 μm PVDF membrane (BIO-RAD) ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were electrophoresed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) precast 4-20 % Mini-PROTEAN TGX gel (Bio-Rad) to separate the proteins.
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of proteins in Laemmli buffer and protein standard were loaded on 4%-15% Criterion TGX Stain-Free protein gel in running buffer TGS 1XL(all from Bio-Rad) and transferred to a 0.2Lµm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... then 20 μg of protein was resolved on a 4-12% Tris gel and transferred to a polyvinylidene difluoride (PVDF) membrane using the Trans-Blot Turbo system (Bio-Rad) according to standard protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 5-10μg of total proteins were separated on bis-tris polyacrylamide gradient gels (NuPAGE Novex 4-12% Bis-Tris Gel, Life tech, #NW04120BOX) or TGX stain-free gels (BioRad, #4568126) and transferred to PVDF membranes ...
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bioRxiv - Biochemistry 2023Quote: ... The total volume of samples was applied to precast gel (Mini-Protean TGX Gels, 4-15% gel, Bio-Rad, CA, USA). SDS-PAGE was carried out following manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the prepared samples were loaded onto the lanes of a 4-15% Mini-PROTEAN Tris PAGE gel (Bio-rad #4561086). Electrophoresis was carried out at 100 V for about 1.5-2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... 30μg of total protein from each sample was placed into Mini-PROTEAN (R symbol) TGX (™) 4-20% gels (Bio-Rad) and subjected to SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: 10-20% of the samples containing immunoprecipitated ECTs for mass-spectrometry were subjected to SDS-PAGE in 4-20% CriterionTM TGXTM Precast Midi Protein gels (Bio-Rad) for visual assessment ...
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bioRxiv - Microbiology 2023Quote: Proteins were separated by SDS-PAGE on 4-15% gradient Mini-Protean TGX Stain-free gels (cat. no. 4568083, Bio-Rad), transferred onto PVDF membranes (cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 μg of protein extracts were run on a 4%–15% polyacrylamide gels (Mini Protein TGX Stain-Free gels, Bio-Rad) and transferred to PVDF (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Purified CreS controls and equivalent OD600 nm units of cleared cell lysates by centrifugation were resolved on a 4-20% SDS-polyacrylamide gel (Bio-Rad). In this step ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were blocked with 4% bovine serum albumin (BSA) (Gemini Bio, 700-109P) dissolved in Tris-buffered saline (Bio-Rad #1706435) supplemented with 0.025% Triton X-100 (TBST) ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were centrifuged at 18,000 x g for 30 minutes at 4 °C and the supernatant was incubated with SurebeadsTM Protein A Magnetic Bead (Bio-rad) conjugated with rabbit monoclonal DYKDDDDK antibody at RT for two hours ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were mixed with an equal volume of 2X Laemmli sample buffer and incubated for 5 min at 95°C prior to separation by SDS PAGE on Criterion TGX stain-free 4-15% gradient gels (Bio-Rad). Following SDS-PAGE ...
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bioRxiv - Developmental Biology 2023Quote: Cell lysates from ITFG1-Myc-expressing MDA-MB-231 and non-expressing control MDA-MB-231 cells were separated in a 4-20% SDS-PAGE gel (Bio-Rad). The gels were stained with Imperial Protein Stain (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) was carried out using 4 μl cDNA (diluted 1:20) with iQ SYBR Green Supermix (BioRad, CA, USA) and specific primers (Biolegio ...