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Citations for Bio-Rad :
4151 - 4200 of 4939 citations for 4 Phenyl 3 buten 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... different amounts of TTR1st in the range 0.1 1−1.10 μg were loaded in a native 4-15% PAGE gel (Mini-PROTEAN® TGX™ Precast Gel, Bio-Rad). The gel contained colored molecular weight standards (Precision Plus Protein Dual Color Standards ...
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bioRxiv - Cancer Biology 2023Quote: ... Between 40mg and 120mg of each protein sample was boiled in Laemmli buffer with 10% β-Mercaptoethanol and equal percentages of each sample were run on 4-15% polyacrylamide gels (BioRad, 4561086). Resolved proteins were wet-transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained in different cycles overnight at 4°C with the following antibodies: anti-CD3 (clone CD3-12; #MCA1477, Bio-Rad, USA), anti-CD206 (clone E6T5J ...
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bioRxiv - Biochemistry 2023Quote: ... and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678084) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 25 μg of each plasmid construct and 25 μg of donor DNA in 4-mm electroporation cuvettes with three pulses (1500 volts, 25 microfarads) delivered by a Gene Pulser Xcell™ Electroporation System (Bio-Rad). Transfected epimastigotes were cultured in LIT medium supplemented with 20% heat-inactivated FBS until stable cell lines were obtained ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was diluted to a 500 ng/16 μl concentration using water and combined with 4 μl of iScript Reverse Transcription Supermix for RT-qPCR (Biorad, Cat# 1708841) and processed in a thermocycler (MJ Research PTC-200 Peltier Thermal Cycler ...
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bioRxiv - Molecular Biology 2023Quote: ... the sample boiled for five minutes at 95 °C and size-separated by SDS-PAGE using a 4–20% Mini-PROTEAN® TGX™ Gel (Biorad). Proteins were transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Microbiology 2023Quote: Volumes of lysates corresponding to 20 μg of protein were then run on a Criterion XT 4- 12% Bis-Tris Precast Gel (Bio-Rad, 3450124) in MES running buffer (50 mM MES ...
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bioRxiv - Synthetic Biology 2023Quote: ... prestained protein ladder were applied to SDS-PAGE using 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad, Hercules, CA, USA). Western blots were carried out in a Trans-Blot Turbo system using Trans-Blot Turbo Mini 0.2 μm Transfer-Packs (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: 30 to 60 µg of protein obtained from AT2 were separated on 4-20% Criterion TGX Gels (Bio-Rad. Cat. No. 5678093), transferred to PVDF membranes (Trans-Blot Turbo System ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten microliters of sample were pipetted onto SDS gels (4%–15% Criterion TGX Stain-free gels; Bio-Rad Laboratories; Hercules, CA, USA), and proteins were separated by electrophoresis (200 V for approximately 40 minutes) ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Fifteen micrograms total protein extracts were separated by 4–20 % stain-Free SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories). Membranes were blocked using Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2022Quote: ... The same volume of all samples (corresponding to ∼15 µg starting FBS dilution) was separated by SDS-PAGE using 4-20% polyacrylamide Mini-Protean® TGXTM Gels (#456-1094, Bio-Rad) and wet-transferred to Nitrocellulose membranes (#1620112 ...
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bioRxiv - Biochemistry 2022Quote: ... Gel demonstrating protein purity was generated using by loading 1 μg of each protein onto a Mini-PROTEAN TGX 4-20% gradient gel (BIO-RAD, 4561096) and then staining with Coomassie blue.
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bioRxiv - Cancer Biology 2023Quote: ... All samples were mixed with loading buffer containing SDS and β-mercaptoethanol and run on homemade 15% or commercial 4-15% gradient acrylamide gels (Bio-Rad). Semi-dry transfer was performed on a Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled at 100 °C for 5 min and loaded in handcrafted 8% SDS-polyacrylamide gel or 4-12% Criterion™ XT Bis-Tris XT Precast Gels (Bio-Rad) and electrophoresed in 1X Tris/Glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... an aliquot of 25µg of total protein per sample was loaded into 4–20% Mini-PROTEAN® TGX™ precast gels (Bio-Rad) for SDS-PAGE separation ...
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bioRxiv - Genetics 2023Quote: ... 50 µg of whole protein lysate was used for all immunoblots and resolved on 4-20% Mini-PROTEAN TGX Precast Protein gels (BioRad; Cat# 4561094). Protein samples were transferred to 0.2 µm PDVF membranes (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... and equal amounts of protein sample were then loaded onto a 4-20% gradient SDS-PAGE gel (Bio-Rad, cat no. 4561095) and run at 120V before being transferred to a PVDF membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and 10 µl of protein sample was loaded in each well of precast Mini-PROTEAN TGX 4-20% gradient Gel (BIO-RAD 4561096). Following SDS-PAGE ...
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bioRxiv - Bioengineering 2023Quote: ... 15 ug protein were loaded and separated using 4–15% Mini-PROTEAN® TGX™ Precast SDS-PAGE Gels (Bio-rad, #4561086). Samples were then transferred to nitrocellulose membranes for immunoblotting ...
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bioRxiv - Immunology 2023Quote: ... and cell pellets were lysed in 1× Laemmli buffer before proteins were separated on 4-20% Mini- PROTEAN® TGXTM Precast Protein Gels (Bio-Rad) and transferred to a nitrocellulose membrane (Odyssey® nitrocellulose membrane ...
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bioRxiv - Neuroscience 2023Quote: ... were incubated in a boiling bath for 10 min and analyzed by SDS-PAGE on 4–20% precast polyacrylamide gradient gel (Bio-Rad, 4561096). Western blot analyses were performed according to standard protocols using antibodies against MBP (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were loaded on a 10% SDS-PAGE gel made in-lab or on a 4-15% pre-cast TGX gel (Bio-Rad, #4568086), along with the molecular marker ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 mg of whole cell extract or 40-80 mg of liver homogenate was loaded on Mini-Protean TGX protein gel (4-20% or 7.5%) (Bio-Rad, Hercules, CA) and transferred to Immobilon-P membranes by electrophoresis (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: ... alum pellets were resuspended in sample loading buffer and boiled at 95 °C for ten minutes before being applied to a precast polyacrylamide gel (4–20% Mini-PROTEAN®, Bio-Rad). Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Migration of 20 μg of lysate supernatants was performed in 4-12 % gradient or in 10 % SDS-PAGE gels (Bio-Rad Laboratories), transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μg of cell lysates were separated on a 4-20% resolving gel (Bio-Rad Mini-PROTEAN TGX Precast Gel #456-1094), transferred to a PVDF membrane for analysis using anti-FLAG (Sigma Aldrich #F3165 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 7.5% polyacrylamide gels or 4-20% gradient gels (Cat # 4561094 Bio-Rad, Hercules, USA). The gel was then transferred to a nitrocellulose membrane with a pore size of 0.45 µm (Cat # 1620115 ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were then separated by 6.5% (w/v) native polyacrylamide gels at 4 °C and the biotin-labeled probes was captured with ChemiDoc™XRS+ (Bio-Rad). The oligonucleotide probes used in this study are listed in Supplemental Data Set S1.
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bioRxiv - Neuroscience 2024Quote: ... were resolved by SDS-PAGE on 4-15% Mini-PROTEAN TGX Stain Free gels (30 μL, 10 well comb, BioRad, Cat# 4568083) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... were used for real-time PCR with gene-specific primers (Supplementary Table 4) and Tbp as a housekeeping gene on a CFX96™ Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled at 95°C for 5 min and loaded into 4-12% Criterion XT-Bis-Tris polyacrylamide gels (Bio-Rad 3450125). Gel electrophoresis was performed in 1X MES running buffer (Bio-Rad 1610789 ...
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bioRxiv - Biochemistry 2023Quote: ... Boiled lysates were loaded on a 4-15% Tris-glycine gradient gel and run at 150 V using Tris/Glycine/SDS buffer (Bio-Rad, 1610732) until loading buffer reached the bottom of the gel ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were separated by SDS-PAGE on 4–15% gradient Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Watford, Hertfordshire, UK), transferred to PVDF membranes (IPVH00010 ...
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bioRxiv - Cell Biology 2023Quote: ... Between 20-30 µg of protein was electrophoresed in 4-15% miniPROTEAN TGX Stain-Free pre-cast gels (BioRad, 4568084/4568085/4568086) using electrophoresis buffer [24.76 mM Tris Base (American Bio AB02000-05000) ...
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bioRxiv - Physiology 2023Quote: ... 0.03% bromophenol blue) containing 9% beta-mercaptoethanol and separated by Mini-Protean TGX gel 4 - 15% (BioRad, stain-free gels #4568084, 4561035) and blotted onto Amersham Hybond-P PVDF membrane ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Equal amounts (10 μL) of the eluted solution from the streptavidin pulldown were loaded in a 4-20% polyacrylamide gel (Bio-RAD 4561096) and separated with SDS-PAGE at 100 V for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: Protein samples of whole cell extracts (5 μg protein) were loaded on 4-20% SDS-PAGE gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, US) and blotted onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at full speed for 10 minutes at 4⁰C and the total protein concentration in the supernatant was quantified using the detergent compatible (DC) protein assay (BioRad, 500-0114). Equal amounts of protein were mixed with anionic detergent (4x LDS loading buffer ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Plant Biology 2024Quote: ... Absorbances were measured at 412 nm at 0 min and 10 min intervals for 4 h using a microplate reader (iMarkTM Microplate Absorbance Reader, Bio-Rad, Japan) (Tables S4 ...
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bioRxiv - Microbiology 2024Quote: ... The samples were run on a low constant voltage at 4°C for 6 to 8 h in Tris Tricine SDS Running Buffer (Bio-Rad, USA). The peptides were detected by Instant Blue (Expedeon Inc.) ...
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bioRxiv - Cell Biology 2024Quote: Equal protein amounts of cell lysates and mitochondria-enriched fractions were separated using SDS-PAGE gels (PreCast Mini-PROTEAN Tris Glycine TGX, 4-15% (Bio-Rad #4561083); PreCast Mini-PROTEAN Tris-Glycine TGX 7,5% (Bio-Rad #4561026 ...
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bioRxiv - Plant Biology 2024Quote: ... The indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-protean® TGX™ precast gels, [Bio-Rad] ...
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bioRxiv - Cell Biology 2024Quote: ... After running the samples by an SDS polyacrylamide gradient gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and transferring them to a PVDF membrane (350mA/1h) ...
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bioRxiv - Microbiology 2024Quote: ... The samples were run on a low constant voltage at 4°C for 6 to 8 h in Tris Tricine SDS Running Buffer (Bio-Rad, USA). The peptides were detected by Instant Blue (Expedeon Inc.) ...
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bioRxiv - Plant Biology 2024Quote: ... Absorbance was measured at 412 nm at 0 min and 10 min intervals for 4 h using a microplate reader (iMarkTM Microplate Absorbance Reader, Bio-Rad, Japan), and AChE activity was calculated as % inhibition ...
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bioRxiv - Plant Biology 2024Quote: ... Seven and a half μl of each super-natant were loaded on a 4-20% TGX Stain-Free precast gel (Bio-Rad, 4568096). Separated proteins were transferred onto PVDF membrane (Bio-Rad ...