Labshake search
Citations for Bio-Rad :
3701 - 3750 of 4939 citations for 4 Phenyl 3 buten 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2020Quote: Organoids fixed in 4% w/v PFA and immunostained were embedded in 1% w/v low melting agarose (Bio-Rad Laboratories) solution and placed in an Eppendorf tube ...
-
bioRxiv - Biophysics 2021Quote: ... fPanx1ΔC purity was assessed by SDS–polyacrylamide gel electrophoresis (PAGE) using 4-20% pre-cast gradient Tris-glycine gel (Bio-Rad) stained with Simply Blue (SimpleBlue Safe Stain ...
-
bioRxiv - Microbiology 2021Quote: ... The mCherry-fusion lysate (input) and proteins eluted from the beads were separated on a 4–20% TGX SDS-PAGE gel (Bio-Rad) and transferred to a PVDF membrane for immunoblot analysis ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein concentration was established using BCA kits (Pierce) and 10µg of protein was loaded into each well of a 4-20% pre-cast gel (BioRad, MiniProtean TGX), flanked with ladder at either end of the gel (PageRuler ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were boiled for 5 min and the proteins were separated in 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-RAD) and electroblotted to nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2020Quote: ... They were lysed in 2x Laemmly buffer (4% SDS, 20% glycerol, 120mM Tris) and resolved by SDS-PAGE gel (BioRad, Country). Proteins were transferred to nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2022Quote: ... were separated via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast 4-20% gradient polyacrylamide gels (Mini-Protean TGX, Bio-Rad). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
-
bioRxiv - Neuroscience 2022Quote: ... size-separated by SDS-PAGE in 4–20% Criterion TGX Precast gels and transferred using Trans-Blot Turbo Midi Nitrocellulose Transfer Packs (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline/0.5 % Tween-20 (TBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... lysates were then clarified through centrifugation (14’000 g, 20 minutes, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
-
bioRxiv - Neuroscience 2022Quote: ... 20 μg of total protein were resolved in 4-20% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo transfer system (Bio-rad). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were resolved by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) using 4-20% precast gel (Biorad, Hercules, CA). Proteins were transferred by semi-dry transfer onto a PVDF (polyvinylidene fluoride ...
-
bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Blots were incubated overnight at 4°C with primary antibodies and then probed with secondary antibodies before ECL development and imaging (Bio-Rad). Primary antibodies used for immunoblotting are as follows ...
-
bioRxiv - Biochemistry 2022Quote: ... cell density was between 4-5 × 106 cells/mL with >95% viability as determined using a TC20 automated cell counter (Bio-Rad). Cells were diluted with warm Expi293 Expression Media to 2 × 106 cells/mL in 42.5 mL ...
-
bioRxiv - Bioengineering 2022Quote: ... Approximately 20 μL of the denatured phage was loaded in a 4-20 % gradient gel (Mini-PROTEAN® TGX Stain-Free gel, Biorad) and separated by electrophoresis at 120 V ...
-
bioRxiv - Bioengineering 2022Quote: ... Proteins were separated on ExpressPlus™ PAGE Ready gels 4 - 20% (A2S, M42015) and transferred on the cellulose membrane (300 mA, 90 min) using a BioRad blotting device (BioRad, USA). After overnight blocking in 5% milk in TBST ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary and HRP-secondary antibodies in TBST buffer with 5% milk (RT for 1 hour or overnight at 4 °C) and revealed with an ECL solution (BIO-Rad) following manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell lysates (10-50 μg) were subjected to SDS-polyacrylamide gel electrophoresis on a 4-20% precast gradient gels (BioRad, 5678094) and proteins were transferred to nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: The source lysates of Brl1 APs 90 min post labeling were adjusted to 50 µL with a protein concentration of 4 µg/µL with lysis buffer as determined by the Bradford method (Bio-Rad). Samples were diluted with 200 µL guanidine chloride (7 M in 100 mM ammonium bicarbonate ...
-
bioRxiv - Cell Biology 2022Quote: ... 25µg of protein was loaded per lane into an SDS-PAGE gel (Bio-Rad Mini-Protean pre-cast gels 4-20%) and gels were run at 160V for approximately 90 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... Equivalent protein samples (cell lysates and DRG fractionized membrane and cytosol) were size separated using 10% or 4-20% SDS-PAGE gels (Bio-Rad), transferred to Immun-Blot PVDF membranes (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... yield and level of purity were determined by SDS-PAGE analysis using 4–20 % or 12 % Mini-Protean TGX precast gels from Bio-Rad. Gels were stained with QC Colloidal stain (3 ng sensitivity ...
-
bioRxiv - Microbiology 2020Quote: ... Cellular protein lysates were resolved on Criterion or 4%–20% Bio-Rad precast SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). Membranes were saturated and proteins were blotted with antibodies in 5% non-fat dry milk ...
-
bioRxiv - Neuroscience 2020Quote: ... heated for 10 min at 70C then run out on a 4-12% Novex NuPAGE gel in 1X NuPAGE MOPS buffer with a protein ladder(Bio-Rad). The gel was transferred to a 0.2um PVDF membrane for 2 hours at 200mA at 4C ...
-
bioRxiv - Molecular Biology 2020Quote: ... 15 μl of the protein extracts were loaded on a 4-12% gradient 26 well Criterion XT Bis-Tris Protein gel (Bio-Rad).
-
bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were centrifuged at 20000 g for 20 min at 4 °C and then filtered by gravity using Poly-Prep chromatography columns (731-1550, Bio-Rad). Supernatants were filtered by gravity through Poly-Prep chromatography columns (731-1550 ...
-
bioRxiv - Molecular Biology 2020Quote: Samples were separated using 10-15% SDS-PAGE gels or 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad, 5678095). Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: Equal amounts of protein from (30 μg, mouse cortex was used) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to nitrocellulose membranes ...
-
bioRxiv - Microbiology 2020Quote: ... 1μg of pKSB vector containing aimed mutation and the bacterial liquid were mixed at 4℃ and electroporated by Micropulser (Bio-Rad) with Ec2 (2.5kV ...
-
bioRxiv - Neuroscience 2020Quote: ... After rinses with PBS-0.1% Tween 20 (PBST), sections were incubated overnight at 4°C with rabbit anti-Iba1 (Wako, 019-19741) and anti-BrdU (Biorad, MCA2060). After washes with PBST ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were separated by electrophoresis on 4-20% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad Laboratories, NSW, Australia) in running buffer (0.1% (w/v ...
-
bioRxiv - Cell Biology 2021Quote: Enriched CCV fractions were resolved via 1D SDS-PAGE on a 4-15% Tris-HCl gradient gel (BioRad cat# 161-1158) at a constant 200V for ∼90 minutes ...
-
bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of each sample were mix with sample buffer loaded and separated using Mini-PROTEAN TGX 4-15% polyacrylamide gels (BioRad: 4561083) and Tris/Glycine/SDS buffer (BioRad ...
-
bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
-
bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini- PROTEAN® TGX Stain-Free™ Gels (BioRad; Cat#17000435).
-
bioRxiv - Biochemistry 2021Quote: ... The cell extract was clarified by filtering through 0.4 µm and 0.22 µm filters prior to loading onto ~4 mL of Ni-NTA resin (Profinity™ IMAC resin, Bio-Rad). To allow the His-tag protein to bind the resin it was incubated overnight at 4°C on a rolling shaker ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 µL per reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 µl reactions ...
-
bioRxiv - Neuroscience 2021Quote: Equal volumes of ISF were boiled at 98°C for 5 min in 50 mM of DTT and 1X LDS and separated by electrophoresis on 4-12% Bis-Tris Precast Gels (BioRad #3450124). Proteins were transferred onto 0.2 μm PVDF membranes (Biorad #1704157 ...
-
bioRxiv - Neuroscience 2022Quote: ... 10µg of protein per sample was loaded into 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and run at 105V for 90 minutes ...
-
bioRxiv - Physiology 2022Quote: ... we ran similar amounts of protein per lane (~10-20 μg, depending on target) on a 4-20% Criterion TGX stain-free gel (Bio-Rad) at 200 V for 50 min with the cassette surrounded by ice ...
-
bioRxiv - Immunology 2022Quote: ... boiled for 10 min and subjected to protein separation by SDS-PAGE in 4 – 20% Mini-PROTEAN TGX precast gels (Bio-Rad) before transferred to nitrocellulose membranes (Wako) ...
-
bioRxiv - Microbiology 2022Quote: ... cells were fixed in 10% neutral buffered formalin at 4°C overnight and then mCherry-positive plaques were visualized using a ChemiDoc MP imaging system (Bio-Rad). Afterwards ...
-
bioRxiv - Biochemistry 2022Quote: ... Both input and IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed by Western blot using specific antibodies.
-
bioRxiv - Biochemistry 2022Quote: ... Input and MOV10/IgG IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed through Western blot using specific antibodies.
-
bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
-
bioRxiv - Developmental Biology 2022Quote: ... The suspended eggs and siRNA (5 μg/μl stock solution, in MilliQ) were transferred to a 4 mm cuvette (Bio-Rad) with a final volume of 200 μl and were gently mixed ...
-
bioRxiv - Biochemistry 2019Quote: ... The input and IP samples were run on 4-12 % SDS-PAGE and transferred to PVDF membranes (Bio-Rad, 162-0177) using Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell (BioRad ...
-
bioRxiv - Genomics 2019Quote: ... PCR products were separated on a 4% agarose gel and the length of the products was analysed using the Image Lab version 5.0 software (Bio-Rad Laboratories). PCR products were purified with QIAquick Gel Extraction kit (QIAgen ...
-
bioRxiv - Microbiology 2019Quote: ... 4 µl of the diluted DNA sample was added to 6 µl of a mixture containing SYBR Green Supermix (Bio-Rad) and gene-specific primers (0·4 µM total ...