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Citations for Bio-Rad :
4001 - 4050 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and resolved for 4 hours at 120V for LapA and 3 hours at 115V for MapA in Mini-PROTEAN Tetra Vertical Electrophoresis Cells (Bio-Rad) with 1x Tris/Glycine/SDS buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell lysates and eluates from immunoprecipitations were run on 10% SDS-polyacrylamide gels and transferred to supported nitrocellulose membrane (Bio-Rad) by standard methods ...
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bioRxiv - Bioengineering 2023Quote: ... followed by an additional wash and resuspension in Assay Buffer for readout by high-throughput cytometry (Bio-Rad ZE5 cell analyzer). Results were decoded using Nomic software ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5×106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The protein samples were separated in 4 −15% acrylamide gels by SDS-PAGE using a Bio-Rad Mini-Protean Tera cell system (Bio-Rad). At the same time ...
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bioRxiv - Microbiology 2023Quote: ... the ratio of plasmid to chromosome copy number in Synechocystis 6803 or Synechococcus 7942 cells was estimated by droplet digital PCR using the QX200 system (Bio-Rad). Primer sets (F12/F13 and F14/R15 ...
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was extracted with RIPA buffer and analysed by SDS-PAGE followed by wet transfer using a Mini Trans-Blot® Cell (BioRad). Antibodies used for Western blots were ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from ∼10 x 106 cells of each experiment using PureZOL RNA Isolation reagent following the manufacturer (BIO-RAD) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... For Western blotting equal amounts (up to 80 μg) of total cell lysates were loaded on SDS–polyacrylamide gel electrophoresis system (Bio-Rad) and transferred on nitrocellulose membrane which was proceeded for immunoblotting to detect specific proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50 μl of competent cells were mixed with 500 ng of the PCR fragment in an ice-cold 0.2 cm cuvette (Bio-Rad Inc.). Cells were electroporated at 2.5 kV with 25 mF and 200 Ω ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
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bioRxiv - Plant Biology 2024Quote: Samples were loaded onto 7.5% or 15% SDS-PAGE gels and electro-transferred with Trans-Blot® SD Semi-Dry Transfer Cell (BIO-RAD) at 18 V for 45 minutes ...
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bioRxiv - Physiology 2024Quote: ... Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps (4°C overnight) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purity and size of the proteins were validated on an SDS–PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20 °C.
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from cells using GeneJet RNA Purification kit (Thermo Fischer) and cDNA was synthesized using iScript Reverse Transcription Supermix (Bio-Rad), as previously described (27) ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections of HEK293 cells were conducted via electroporation using the Bio-Rad Gene Pulser Xcell system (Bio-Rad, Hercules, CA, USA) in 4 mm cuvettes with OPTI-MEM medium ...
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bioRxiv - Cancer Biology 2024Quote: Pellets of 1×106 treated cells were incorporated in 1% agarose at 50°C and cast into molds (Biorad, Grabels, France) avoiding air bubbles ...
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bioRxiv - Cell Biology 2024Quote: Cell growth in liquid media was monitored in a 96-well flat-bottomed plate using a microplate reader (iMark, Bio-Rad). Cells were grown to the mid-log phase and then diluted to OD600 = 0.1 ...
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bioRxiv - Biochemistry 2024Quote: ... or a combination of both was added for 6 hours prior to imaging with either a ZOE Fluorescent Cell Imager (Bio-Rad) or EVOS FL Cell Imaging System (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cell-DNA mixture was transferred to a chilled electrocuvette and pulsed using a Gene Pulser Xcell Electroporation System (Bio-Rad) set to 1800 V ...
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bioRxiv - Biochemistry 2024Quote: ... using gels with a concentration of 8%, 10%, 14% or 15% acrylamide (Carl Roth, 3029.1) in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell system (Bio-Rad, 1658029FC) with running buffer containing 0.2 M glycine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... pestis whole cell lysates were prepared by lysing bacteria (grown at 37°C in LB-MOX) in Laemli buffer (Bio-Rad) containing ß-mercaptoethanol at 95°C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: Organoids and Mode-K cells were incubated at 37°C with 5% CO₂ in culture media supplemented with 5 µg/mL PureBlu Hoechst (Bio-Rad) and 5 µg/mL Propidium Iodide ...
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bioRxiv - Immunology 2024Quote: Cell samples were analyzed for the cell number and viability either with Nucleocounter NC-100 (Chemometec) or with trypan blue and TC-20 (Bio-Rad) automated cell counter ...
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bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
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bioRxiv - Microbiology 2024Quote: ... The cell mixture was transferred to a 1 mm electroporation cuvette and pulsed using a Gene Pulser Xcell Electroporation System (Bio-Rad) using 2.1 kV/cm ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were immediately lysed in ice cold Radioimmunoprecipitation Assay (RIPA) buffer and protein was quantified using a Bradford Assay (Bio-Rad), per manufacturer’s instructions.
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Treated whole cell extracts (5 µg per lane) were resolved on a 12% TGX Stain-free polyacrylamide gel (Bio-Rad, #1610185) by applying 180V until loading dye ran off the gel ...
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bioRxiv - Cell Biology 2022Quote: ... the number and purity of nuclei as assessed by size was determined using an automated cell counter (TC20; Bio-Rad, Hercules, CA). If drug treated ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid 86613-RIOK2-gRNA and single-stranded donor templates for introduction of ouabain resistance and either RIOK2S483A or RIOK2S483D point mutations were electroporated into eHAP1 cells at 300 V with a Gene Pulser System (Bio-Rad Laboratories) in cuvettes with a 4-mm inter-electrode distance (Eurogentec) ...
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were resolved by SDS-PAGE 12% self-cast gel and transferred onto polyvinylidene difluoride (PVDF) membrane using the Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) in transfer buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2019Quote: ... Klf4 (a marker of stemness [72]) and Hes1 (another known stem cell marker [73]) were measured using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories) according to the manufacturer’s protocols in a Bio-Rad CFX96 Real-Time PCR detection system (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in cell free culture medium or serum samples were measured using the Bio-Plex Pro Assay System (Bio-Rad Laboratories) [23] ...
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: eGFP fluorescence was analysed by flow cytometry measurements for bioreactor and shake flask cultivation applying S3e™ Cell Sorter (Bio-Rad, Germany) applying a 488 nm laser in combination with a 525/30 nm filter ...
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bioRxiv - Cancer Biology 2020Quote: ... To reduce non-specific binding single cell suspensions were incubated for 5 min at 4°C with PBS/10% AB-serum (Bio-Rad, Germany), subsequently stained with fluorescence-labeled or biotinylated antibodies for 15 min at 4°C and washed once with PBS/2% FCS/0.01% NaN3 ...
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bioRxiv - Biochemistry 2020Quote: ... the resulting dinucleosomes were purified from aberrant or non-ligated mononucleosomes by a second preparative native gel system (Biorad 491 prep cell). In contrast to (Poepsel et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were blotted on 0.45 µm nitrocellulose membrane (Amersham) overnight at a constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA). Membranes were decorated using anti-mCherry-1C51 (Abcam) ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmids were coated on tungsten beads and used for biolistic transformation of the cells with the PDS-100/He system (Bio-Rad, 1672257). DNA from zeocin (100 µg/mL final ...
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bioRxiv - Plant Biology 2021Quote: Protein bands were run in a 15% polyacrylamide gel and transferred to a PVDF membrane using a mini trans-blot cell (Bio-Rad, USA). The membrane was stained with Coomassie blue R-250 and the bands were cut with a sterilized scalpel ...
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bioRxiv - Microbiology 2019Quote: ... the samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using the mini-PROTEAN II cell electrophoresis system (Bio-Rad, USA). The proteins were denatured in 2× loading buffer at 100°C for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: The cell harvesting and extraction of proteins followed the method described previously.10 A protein assay kit (Bio-Rad, Hercules, CA, USA) was used to determine protein concentrations ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from freshly isolated or cultured cells using the Aurum Total RNA Mini Kit (732-6820, Bio-Rad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were loaded on 4-12% Bis-Tris gel and separated by SDS-PAGE on the Criterion system (Bio-Rad Laboratories), and electrophoretically transferred to nitrocellulose membrane ...
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bioRxiv - Microbiology 2022Quote: ... or in-house prepared 15% polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories). The membranes were incubated in blocking buffer [5% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... in transfer buffer (50 mM Tris, 40 mM glycine, and 20% methanol) using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) at 25 V for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... Electrophoresis was carried out for 1 hour at 90 V using an electrophoresis system (mini–DNA SUB CELL, Bio-Rad, Mississauga, Canada). The DNA fragments were visualized in a Gel Doc XR+ Gel Documentation System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Equal total protein was loaded from each of the indicated cell lysates and run on a Criterion™ XT 4-12% Bis-Tris protein gel (Bio-Rad). Protein was transferred at 4°C to nitrocellulose membrane for 2 hours at 50V ...